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Research Journal of Life Science

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Published by Universitas Brawijaya

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Welcome to the Research Journal of Life Science, published by the Institute of Research and Community Service UB. For further information please contact our editorial office.

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Articles 187 Documents

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Komposisi Serangga Kanopi di Kebun Apel di Poncokusumo, Malang dan Bumiaji, Batu Amin Setyo Leksono; Bagyo Yanuwiadi; M Asmuni Hasyim; Frank Leonardo Apituley
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This research abjective is to analyze the difference of abundance, diversity and composition of canopy insect in Poncokusumo and Bumiaji on blossoming and fruiting seasons. Insect compostions were observed by regular sampling using blue and yellow water pan trap. Water pan traps were suspended on apple stands at 2 m height. Sampling efforts was done by block system on 5 trees with 4 times collection, resulting 20 replications. Difference of mean was tested using general linear model analysis of variance. The canopy insect compositions between location were compared using Bray-Curtis similarity index. Overall, canopy insect diversity in two study sites was low. The abundance and diversity of canopy insect in Poncokusumo was higher than that in Bumiaji. The abundance and diversity of canopy insect in yellow traps was higher than that in blue traps. The effect of flowering phenology was significant on the overall abundance but not for the diversity. The effect of study locations on the abundance interacted with season. This meant there was a seasonal variation afecting location.

Adiposopathy In Vitro Study The Effect of Toxoplasma Gondii Profilin Induction To The Expression of IL-6 and TNF-a as A Predictor Candidate of Adipocyte Dysfunction on Subcutan Adipocyte Culture Sudjari Sudjari; Hendra Susanto; Rasjad Indra
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Metabolic syndrome is a group of several risk factors consist of hypertension, dyslipidemia, glucose intolerance disorder, obesity, and has a relationship with CVD pathomechanism. WHO was report in 2003 more than 300 million adult in the world suffered from obesity. The role of adipose tissue in infectious diseases and adipocyte quality changes after exposure to pathogen has not been revealed. On the other hand, the prevalence of infectious diseases in developing country is still high. Previous studies proved a relationship between infection of Toxoplasma gondii and tissue dysfunction. Profilin is a membrane component of Toxoplasma gondii that able to stimulate expression of proinflammation cytokines through TLR-11 upregulation, and predicted able to trigger the adipocyte dysfunction. The aim of this study are to knows the effect of profilin exposure in vitro on subcutaneous adipocyte to the expression of IL-6 and TNF-α. The measurement of IL-6 and TNF-a levels was done by ELISA technique. The result showed profilin exposure doses 5, 15 and 25 ng / ml increasing IL-6. Profilin exposure cause adipocyte dysfunction through the increasing proinflammatory adipocytokine levels as pathomechanism of metabolic syndrome associated with adipose tissue.

Genetic Polymorphism at Acaca Locus and Its Relationship With Productive Performances in Ettawa Crossbred Goat Sucik Maylinda; Tri Eko Susilorini; Puguh Surjowardojo
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Research with aim to estimate genetic polymorphism at ACACA (Acetyl-coenzyme A carboxylase ) locus in Ettawa Crossbred goat wan its relationship with production traits was done at goat population in Batu, Lawang and Ampel Gading. 46 female goats were taken it’s blood sample to isolate the DNA and continue with PCR (Polymerase Chain Reaction) and RFLP (Restricted Fragment Length Polymorphism). PCR was used to amplify ACACA gene fragment in intron 3’ about 200 bp with primer F : 5’ – AGT GTA GAA GGG ACA GCC CAG C – 3’ and R : 5’ – GTG GAA TGA CAC ATG GAG AGG G – 3’; RFLP was used to test mutation of that fragment in particular place (point) using restriction enzyme RSA1. Variables were alelles and genotypes composition in population, milk and fat content, and birth weight of kid. Result showed that (a) genetic polymorphism at locus ACACA in three location was high that is 44,22 %, with allele frequency of G (p) = 33 % and allele T (q) = 67 %; (b) no relationship between the high polymorphism with productive performance of goat in fat and protein content, and birth weight of kid. It was concluded that in goat population there was a high polymorphism at ACACA gene, and that polymorphism was not related to production.

Respon Proliferasi, Diferensiasi dan Ekspresi C/EBPa Akibat Paparan Quercetin pada Kultur Preadiposit Tikus (Rattus Norvegicus) Strain Wistar Secara In Vitro Retty Ratnawati; Satuman Satuman; Tinny Endang Hernowati
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Introduction. Obesity is a metabolic syndrome as risk factor to the cardiovascular disease. Obesity occurs caused by adipocyte massive that is started by proliferation and differentiation of preadipocytes that involves transcription factors such as C/EBPα ,PPARγ and SREBP-1. Is known the TNF α and [Ca2+]i Increase when adiponectin decrease at the end of adipocyte differentiation. Therefore it is a deemed treat the obesity through the inhibition of adipocyte proliferation and differentiation using natural resources. Quercetin in due of the flavonoid found in apple,vegetables etc. The aim of this study is to prove the affectivity of quercetin to inhibit proliferation and differentiation of preadipocyte through inhibited of C/EBPα in rat preadipocytes culture method.Methods. Their study was a laboratory experimental. Quercetin was exposed to the preadipocytes human culture, after it was induced by differentiation stimulator the doses quercetin was grouped in: control, 50 µM (Q50), 125 µM (Q125) and 625 µM (Q625) groups. The amount of proliferation and differentiation was descriptive analyzed. The expression of C/EBPα, were identified by Immunocytochemistry and western blotting.Result. Quercetin 100 inhibited the rate of proliferation cell, while quersetin 625 inhibited differentiation in preadipocytes culture. There was a dose dependent to quersetin towards the decrease of PPARγ expression and C/EBPα expression in this preadipocytes culture.Conclusion. It is conclusion that quersetin inhibits proliferation and differentiation preadipocytes culture through the decrease of the expresion of C/EBP α.

Hubungan Kadar Vitamin D Dengan Jumlah Set T Regulator pada Pasien Lupus Eritematosus Sistemik Handono Kalim; Kusworini Handono; Dian Hasanah
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Latar Belakang. Lupus Eritematosus Sistemik (LES) merupakan penyakit autoimun yang semakin sering dijumpai dengan angka kematian tinggi. Etiopatogenesis penyakit ini belum begitu jelas, meskipun telah ditunjukkan adanya abnormalitas berbagai sel-sel imun termasuk hiper-reaktivitas sel T, sel B, sel dendrit; dan menurunnya fungsi sel T regulator (Treg). Vitamin D memiliki peran dalam pengaturan sistem imun. Penelitian pada ras Kaukasia menunjukkan bahwa kadar vitamin D yang rendah berkaitan dengan timbulnya penyakit autoimun termasuk LES. Peran vitamin D pada sistem imun pasien LES masih banyak kontroversi. Penelitian ini bertujuan untuk mengetahui hubungan kadar vitamin D dengan jumlah sel Treg.Metoda Penelitian. Penelitian ini adalah penelitian kasus-kontrol yang membandingkan jumlah sel Treg pada pasien LES dan kontrol sehat dengan melihat status vitamin D-nya. Kadar vitamin D pasien LES diukur dengan metoda ELISA, jumlah sel Treg (CD4+,CD25+,FoxP3+) ditentukan dengan metoda flowcytometri.Hasil. Kadar vitamin D pada pasien LES lebih rendah daripada kontrol sehat (p=0,000) dan didapatkan korelasi negatif antara status vitamin D dengan persentase sel Treg (koefisien korelasi -0,358 dan p=0,006). Hal ini mungkin merupakan umpan balik positif untuk menanggulangi hiperreaktifitas dari sel T dan sel B.Kesimpulan. Status vitamin D berkorelasi negatif dengan persentase sel Treg.

Estimasi Distribusi Klorofil-A di Perairan Selat Madura Menggunakan Data Citra Satelit Modis dan Pengukuran In Situ Pada Musim Timur Bambang Semedi; Nur Maulida Safitri
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Penelitian ini bertujuan untuk menganalisis pola konsentrasi klorofil-a dari citra satelit Aqua MODIS pada musim timur, menganalisis berbagai parameter yang memberikan dampak dominan terhadap nilai klorofil-a serta melakukan validasi nilai konsentrasi klorofil-a dari citra satelit Aqua MODIS terhadap nilai konsentrasi klorofil-a data in situ. Distribusi klorofil-a di perairan Selat Madura pada Musim Timur diselidiki berdasarkan data citra satelit Aqua MODIS bulan Juni-September 2013 serta pengukuran klorofil-a in situ pada bulan September 2013 menggunakan metode spektrofotometri.Hasil penelitian menunjukkan bahwa parameter oseanografi yang berkaitan dengan persebaran klorofil-a di perairan Selat Madura diantaranya adalah suhu permukaan laut, kecerahan, oksigen terlarut dan kecepatan arus. Sebaran klorofil-a pada musim timur berkisar antara 0,022317 – 1,561958 mg/L dengan puncak sebaran tertinggi di bulan Juli yaitu berkisar antara 0,102566 – 1,561958 mg/L. Setelah dilakukan uji validasi terhadap hasil analisa citra dan in situ menggunakan uji regresi, diperoleh nilai R square sebesar 80,14% sehingga dapat disimpulkan hasil analisa citra satelit berpengaruh besar terhadap hasil analisa in situ sebesar 80% dengan rata-rata konsentrasi klorofil-a hasil analisa citra 1,127181 mg/L dan rata-rata konsentrasi klorofil-a in situ 1,195664 mg/L. Kedua data memiliki nilai rata-rata pada rentang 1-1,5 mg/L sehingga dapat disimpulkan perairan Selat Madura memiliki kandungan klorofil-a yang tinggi.

Kualitas Lingkungan Tambak Insentif Litapenaeus Vannamei Dalam Kaitannya Dengan Prevalensi Penyakit White Spot Syndrome Virus Maimunah, Yunita; Kilawati, Yuni
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Increasing number of vannamei shrimp (Litapenaeus vannamei) ponds are switching from traditional to intensive farming systems, the more impact resulting among other potential environmental pollution. Pollution of the environment can directly degrade water quality cultivation and facilitate access of pathogens to infect the host. In this study examines how the quality of the environment, population and genetic characteristics of shrimp that live in some intensive pond associated with a disease that often affects farmed shrimp is White Spot Syndrome Virus (WSSV). Acquisition and primary data collection is done by conducting interviews and direct observation in the measurement of water quality parameters of both physics and chemistry and morphology observation of shrimp as well as the ICP11 gene expression detection of WSSV disease in vannamei shrimp DNA in the laboratory.

Kelayakan Kualitas Perairan Sekitar Mangrove Center Tuban Untuk Aplikasi Alat Pengumpul Kerang Hijau (Perna viridis L.) Syarifah Hikmah Julinda Sari; Ledhyane Ika Harlyan
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The coastal waters around the Mangrove Center Tuban, East Java is a potential habitat for the green mussel (Perna viridis L.). Therefore, the water quality of this area need to be assessment. The study was aimed to determine the feasibility of water quality in coastal water f Mangrove Center Tuban for application collecting gear of green mussels. The sampling was conducted by set up three stations randomly in studied area. Parameters was measured including temperature, pH, salinity, DO, depth, turbidity, BOD and TSS. The results showed that the coastal waters around the Mangrove Center Tuban, owned temperature that ranged from 29.10 to 30.67 0C, the pH ranged from 8.23 to 8.37, salinity ranged from 26.87 to 30.30 ‰, DO fall in the range of 6,63- 6.87 mg / L, the water depth at the time of measurement ranges from 40-85 cm. TSS ranged from 206.2 mg / L to 353.7 mg / L, BOD value between 4.05 to 12.2 mg / L, while the turbidity ranged from 134 to 400 NTU. Parameters that below the standard namely temperature, pH, DO, salinity and BOD, while TSS and turbidity were exceed the threshold quality standards set by the government.

The Effect Of Solid Fertilizers And Biourine Application On Plants Rice CV Ciherang at Ngujung, Batu, East Java Mudji Santosa; M. Dawam Maghfoer; Sisca Fajriani
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Ciherang rice is one of the national rice seed production with an average production and potential yield 6 and 8.5 tons/ha respectively. In case of farmers in Batu, the actual production was lower than the average production of Ciherang rice, however there were many natural resources which can support production. Research was aimed to determine the influence of application of synthetic fertilizers and biourine in rice cv Ciherang on farm. It was conducted on Andisol soil, Ngujung, Batu, ca. 24 km from the city of Malang to the northwest at 900 m above sea level (m asl), and average temperature of 22°C. Research was conducted from June to October 2012, by a factorial randomized block design with 2 factors. First factor was application of biourine with 0 (TB) and 1000 l/ha (B). The last factor was application of 6 combinations of fertilizers i.e.: 1) 1st combination of NPK, 150 kg N/ha (urea): 50 kg P2O5/ha ( SP36 ): 70 kg K2O/ha (KCl), 2) 2nd combination of N(50 kg N /ha ( urea ), 25 kg P2O5 /ha (SP36) and 35 kg K2O/ha (KCl), 3) 600 kg/ha of Phonska (NPK; 15 :15 :15), 4) 300 kg/ha of Phonska (NPK; 15 :15 :15), 5) 20 tons/ha of cow manure and, 6 ) 10 tons/ha of cow manure. This experiment was consisted of 12 combinations of treatments and repeated three times. Results showed that averages of plant height, Leaf Area Index (LAI), time of flowering, time of harvesting, the number of tillers plant, number of panicles per culm, number of grains and 1000 grain weight were 88.8 cm, 4.39, ca. 60 days after planting (DAP), 125 DAP, 23.9 plants, 21.6, 152.7 grains/panicle and 26.8 g, respectively. The average highest yield of rice was 1351.7 kg/m2 (by 1st combination of NPK, 150 kgN/ha (urea), 50 kg P2O5/ha (SP36) and 70 kg K2O/ha (KCl) plus 1000 liters of biourine application and the average lowest yield was 796.7 kg/m2 which treated by10 tons/ha cow manure.

Mutual Partnership Model between Plant Breeder and Gapoktan-Ponpes (A Traditional Farmer Group) in Pre-releasing New Hybrid and Composite Varieties of Sweet Corn Arifin Noor Sugiharto; Agus Suryanto
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

We have been genotyping the Filius (F6) sweet corn lines that currently ready to be evaluated by multi-location and heterosis test.Normally, in the step of breeding program, releasing varieties followed by seed production and distribution would be done after such tests that costly needed and time consuming. Traditional institution of farmer group called in Indonesia as “Gapoktan” and the Religious teaching stage called as “Ponpes” have been known as non-formal institution which have high potency and unique networking access to surrounding public citizen and other informal institutions. As so did, they can be as a producer and virtual guarantor or good marketing agent of many agricultural products as well. Therefore, a mutual partnership model established between breeder and them is very important and to be a prospective idea to short cut breeding process and hybrid seed production. Scientific accountability and efficiency of whole breeding – seed production would be kept into main consideration. The concept was “Gapoktan” and “Ponpes” firstly would be trained to adopt technology for crossing to set F1 Hybrid seed, then the results would be evaluated and compared with those done by breeder to determine whether they eligible or not. If they did so, partnership would be mutully continued and they would be encouraged to distribute F1 seeds to other “gapoktan” or public citizens. Three nominated Gapoktan and Ponpes from separated regencies, i.e., Pasuruan, Malang and Batu were determined as the model.The results showed that F1 seed production variable as indicator achievement of them were not significantly different from those of breeder in any place. Even though in between the places they did differ in several vegetative and productive factors each other. The differences were probably caused by ecological factors, not by their capability in agriculture practicing. Based on standard of common farming of corn or sweet corn in East Java, benefit gain increment by farmer caused by such partnership in F1seed production reached about 150% to 247%. So that, partnership model for producing multi-location test as well as producing hybrid seeds between Breeder-Gapoktan-Ponpes was economically prospective and scientifically eligible to do so. On the benefit consideration gained by farmers, Pasuruan was the place where the most attractive to be done partnership model followed by Malang and Batu.

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