American rope leaves (Mikania micrantha Kunth.) contains secondary metabolites such as flavonoids, tannins, saponins and steroids. This study aimed to observe antifungal activity of ethanol extract 96% of american rope leaves were 200 mg/mL, 400 mg/mL, and 600 mg/mL concentration against Trichophyton mentagrophytes by disc diffusion method on Sabouraud Dextrose Agar (SDA) medium. Ketoconazole were positive control. The results showed that the 96% ethanol extract of american rope leaves had antifungal activity against Trichophyton mentagrophytes. The minimum inhibitory concentration of 96% ethanol extract of american rope leaves against Trichophyton mentagrophytes at 100 mg/mL.
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