Indonesian Biodiversity Journal
Vol. 2 No. 2 (2021): Agustus 2021

ANTAGONIST, ANTIBACTERIAL ACTIVITY OF Stapylocossus aureus AND ISOLATE OF ORAL BACTERIA FROM THE ENDOGENOUS FUNGUS Apis dorsata BINGHAMI NEST

Dina Rombot (Medical Education Study Program, Faculty of Medicine, Sam Ratulangi University)
Ellen Adil (Biology Department, Faculty of Mathematics and Natural Sciences, Manado State University)
Mario Walean (Pharmacology Laboratory, Pharmacy Study Program, Prisma University Manado)
Virjinia Bastian (Students of Biology Study Program, Faculty of Mathematics and Natural Sciences, Manado State University)

Article Info

Publish Date
15 Aug 2022

Abstract

Apis dorsata Binghami is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The study consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six fungal isolates from Apis dorsata Binghami's nest, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB1, FAB2, FAB3, FAB4 and FAB5 were antagonistic. The results of the antibacterial test showed that all isolates produced an average bacterial growth inhibition of 17-18 mm on Staphylococcus aureus and isolates of oral bacteria. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.

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Journal Info
Indonesian Biodiversity Journal
Website

Abbrev

ibj

Publisher

Universitas Negeri Manado

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

Description

Indonesian Biodiversity Journal (IBJ) (ISSN: 2722-2659) is an international peer-reviewed and open access journal that publishes significant and important research from all areas of biological science fields such as biodiversity, biopharma, DNA barcoding, and molecular biology, ecology, physiology, ...