Jurnal Indonesia Sosial Sains
Vol. 5 No. 07 (2024): Jurnal Indonesia Sosial Sains

Pengukuran suhu, pH dan kadar substrat terhadap aktivitas enzim Galactose-1-Phosphate Uridyltransferase

Sisca Sisca (Universitas Trisakti)
Edy Parwanto (Universitas Trisakti)
Monica Dwi Hartanti (Universitas Trisakti)
David Tjahyadi (Universitas Trisakti)

Article Info

Publish Date
25 Jul 2024

Abstract

Galactose-1-phosphate uridyltransferase (GALT) is an important enzyme involved in galactose metabolism. Understanding the factors influencing GALT activity is critical to elucidate its physiological role and potential therapeutic implications in galactosemia. In developing new drugs, chicken intestine/liver powder can be used as an enzyme source, GALT, to treat galactosemia. Therefore, it is necessary to research the characterization of the GALT enzyme in chicken intestine and liver powder. In this study, we investigated the influence of temperature, pH, and substrate level on GALT enzyme activity using an experimental approach in vitro. The optimum pH extraction results show that the optimum pH for the extraction of the chicken intestine and liver GALT is pH 7, with activity values ​​of 0.47 units/mL and 0.3953 units/mL, respectively. The optimum temperature for the extraction of chicken intestine and liver GALT is 37℃ with substrate hydrolysis capabilities of 0.48 U/mL and 0.57 U/mL, respectively. Meanwhile, the optimum substrate content is 400x. These insights provide a valuable foundation for further research aimed at comprehensively understanding GALT function, developing targeted interventions for disorders of galactose metabolism, and possible application in the development of new drugs for galactosemia

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Journal Info
Jurnal Indonesia Sosial Sains
Website

Abbrev

jiss

Publisher

CV. Publikasi Indonesia

Subject

Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Neuroscience Physics Social Sciences

Description

The Jurnal Indonesia Sosial Sains is a scientific journal in the form of research and can be accessed openly. This journal is published once a month by CV. Publikasi Indonesia. The Jurnal Indonesia Sosial Sains provides a means for ongoing discussion of relevant issues that fall within the focus and ...