Indonesian Journal of Chemical Science
Vol. 13 No. 1 (2024)

Optimization of Microplate Type Uv-vis  Spectrophotometer Performance as an Antioxidant Activity Testing Instrument

Martin Sulistyani (Universitas Negeri Semarang)
F. Widhi Mahatmanti (Unknown)
Nuril Huda (Unknown)
Ridho Prasetyo (Unknown)



Article Info

Publish Date
28 May 2024

Abstract

Eliminating a compound's capacity to impede and neutralize free radical-induced oxidation processes is the goal of antioxidant activity analysis. The DPPH (pikrilhidrazil-2,2-difenil-1) method was the approach taken in this investigation. Simple, sensitive, stable, quick, straightforward, and suitable for usage with tiny sample volumes are some of the benefits of DPPH procedures. In this investigation, some plant extracts were employed as a comparison, with ascorbic acid serving as the control. In order to evaluate a microplate-type uv-vis  Spectrophotometer effectiveness as an antioxidant activity test tool, the study will determine its maximum wave, linearity, accuracy, and IC50 values. Wavelength selectivity is found in 517 nm. The results validation of the DPPH method for analyzing the activity of antioxidants. Measurements of the ascorbic acid solution as a control obtained R2 values of 0.9781 compared to the LoD and LoQ limits of 0.961 and 3.053 respectively. The IC50 of ascorbic acid, ginger and turmeric, respectively, was 14.7218 (strong), 29.9194 (very strong), and 52.4943 (strong). 

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Journal Info

Abbrev

ijcs

Publisher

Subject

Chemistry Physics

Description

Indonesian Journal of Chemical Science publishes basic research articles and conceptual of Chemistry, issued three (3) times a year, overall 10 articles per issued. To commemorate important events and agenda, may be issued a special edition that will include 10 articles in each ...