Al-Kimia
Vol 4 No 1 (2016): June

Sintesis Heptapeptida Linear (H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Oh) dengan Menggunakan Dic/Oksima sebagai Reagen Pengkopling

Rani Maharani (Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran)
Eka Fitri Yanti (Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran)

Article Info

Publish Date
03 Jan 2016

Abstract

A tetrapeptide, an analog of  trypsin-modulating oostatic factor (TMOF), has been synthesised by using a method of solid-phase peptide synthesis on 2-chlorotrityl chloride resin. Fmoc strategy was applied on the synthesis. The formation of peptide bond was facilitated by DIC/oxime as coupling reagent. After all of amino acids were attached on the resin, tetrapeptidyl resin was added by a mixture of TFA:water:EDT(90:5:5) in dichloromethane to cleave the peptide.Crude peptide was purified by reverse-phase column chromatography and the purified peptide was analysed by TOF ES-MS spectroscopy.

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Journal Info
Al-Kimia
Website

Abbrev

al-kimia

Publisher

Universitas Islam Negeri Alauddin Makassar

Subject

Chemistry

Description

Al-Kimia is a journal of chemistry that published by Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar. The journal is published twice a year on January-June and July-December. This journal encompasses original research articles in all aspects of chemistry and related ...