Indonesian Journal of Chemistry
Vol 12, No 3 (2012)

ADSORPTION OF AFLATOXIN B1 IN CORN ON NATURAL ZEOLITE AND BENTONITE

Nuryono Nuryono (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281)
Ali Agus (Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta)
Sri Wedhastri (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)
Y.M.S. Maryudhani (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)
Deni Pranowo (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281)
Yunianto Yunianto (Animal Husbandry Academy Brahma Putra, Yogyakarta)
Ebrahim Razzazi-Fazeli (Proteomic Unit, VetCore Facility for Research, University of Veterinary Medicine, Vienna)



Article Info

Publish Date
28 Dec 2012

Abstract

A study on adsorption of AFB1 in corn (kernel and grained) on natural zeolite and bentonite has been investigated. The first work was adsorption in a batch system of standard AFB1 solution on adsorbents. Some factors such as contact time, concentration of AFB1 and particle size of adsorbent were evaluated. The amount of AFB1 adsorbed was calculated based on the difference of AFB1 concentration before and after adsorption determined by high performance liquid chromatography (HPLC) method. Adsorption of AFB1 in corn sample was emphasized by mixing aqueous suspension of sample with adsorbent. Concentration of AFB1 in suspension was analyzed by enzyme-linked immuno-sorbent assay (ELISA) method. Result shows that adsorption of AFB1 on adsorbents of natural zeolite and bentonite is very fast. Within 15 min 99% of AFB1 (200 ng/mL) has been adsorbed by 25 mg of bentonite and 96% by zeolite. The particle size higher than 200 mesh did not give significant effect on the AFB1 adsorption capability. Effectiveness of zeolite in adsorbing AFB1 is lower than that of bentonite. Capability in reducing AFB1 contamination in corn samples (kernel and meal) for both adsorbents is lower than that in standard solution.

Copyrights © 2012






Journal Info

Abbrev

ijc

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...