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All Journal EKSAKTA: Journal of Sciences and Data Analysis Jurnal Perlindungan Tanaman Indonesia Indonesian Journal of Chemistry

Deni Pranowo

Chemistry Department, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada, Yogyakarta

Author-ID : 344796

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology

Published : 11 Documents Claim Missing Document

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Green Chemistry: Effect of Microwave Irradiationon Synthesis of Chitosan for Biomedical Grade Applications of Biodegradable Materials Amri Setyawati; Deni Pranowo; Indriana Kartini
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 16, ISSUE 2, August 2016
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol16.iss2.art8

Microwave assisted chitosan synthesis as biodegradable material for biomedical application has been done. The purpose of this research is to synthesis of chitosan with high DD and low molecular weight using microwave energy, the study of reaction conditions include parameters of power and reaction time. Chitosan was prepared by deacetylation of chitin with 60% NaOH solution. Conventional method has been done by reflux for 90minutes, resulting chitosan with DD of 79.5%, 72.6% yields and molecular weight 6051 g/mol. Green chemistry method using microwave radiation at 800 Watts for 5 minutes has produced chitosan with highest DD, yield and molecular weight of 86%, 75% and 3797 g/mole respectively. Synthesis of Chitosan by microwave radiation method can save 10x electrical energy for the reaction, also rapidly and effectively to produce chitosan with low molecular weight compared to conventional methods

Laporan Baru: Spesies Lalat Buah Terpikat 4-(4-Hidroksi-Fenil)-2-Butanon Deni Pranowo; Edhi Martono; Ahmad Taufiq Arminuddin; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Acetylation of 4-(4-hydroxy-penyl)-3-butene-2-one by anhydride of acetic acid catalyzed by sulphuric acid results in 4-(4-acetoxy-phenyl)-3-butene-2-one compound, and 4-(4-hydroxy-phenyl)-2-butanone compound as a by product. The first compound alone doesn’t attract fruit flies, but a mixture of the two products attracts male fruit flies. This mixture was tested for its attractancy to the fruit flies in Bantul, Sleman and Kulon Progo regencies, Yogyakarta Special Province; along with Cue and Methyl eugenol lures as comparison. The result showed that the mixture was able to attract four fruit flies species, i.e. Bactrocera albistrigata, B. caudata, B. cibodase, and Bactrocera sp1. One genus found has not been able to be identified to species level. Based on its wing morphometric measures, this species is put under Bactrocera dorsalis complex group.

Oviposition Preference of Bactrocera dorsalis Hendel (Diptera: Tephritidae) on Different Fruit in Snake Fruit Orchard Rahmi Fitrah; Deni Pranowo; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.52825

The oriental fruit fly Bactocera dorsalis (Hendel) is an important pest of snake fruit (Salacca zalacca) in Sleman District. Due to the high level of damage by the fruit flies, it is necessary to do. The aim of this research was to find out suitable fruit traps, by testing the oviposition preferences of the fruit flies in the orchard to lay eggs on several types of fruit. The research was done in snake fruit orchard located at Sleman Yogyakarta and owned by farmers, while the Lab works were done at the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. All researches were done between April-June 2019. Guava (Psidium guajava), watery rose apples (Syzygium aqueum), starfruit (Averrhoa carambolae), and snake fruit (Salacca zalacca) were used as trap crops. Each fruit, with the same maturity level, was hung 1.5 m above the ground for 4 days with 12 days total trapping at intervals of 3 times. After the test, each fruit was taken and the insects in it were reared in the laboratory. The number of pupae and flies that emerged from each fruit was counted and compared. The results showed that in the orchard 1 as well as 2, of guava fruit produced the highest number of (151 pupae) followed by salak (94 pupae), star fruit (83 pupae), and water guava (2 pupae). The finding of seeds shows that guava fruit is the most suitable host for the fruit flies to be used in the trapping, followed by star fruit and watery guava.

ADSORPTION OF AFLATOXIN B1 IN CORN ON NATURAL ZEOLITE AND BENTONITE Nuryono Nuryono; Ali Agus; Sri Wedhastri; Y.M.S. Maryudhani; Deni Pranowo; Yunianto Yunianto; Ebrahim Razzazi-Fazeli
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

A study on adsorption of AFB1 in corn (kernel and grained) on natural zeolite and bentonite has been investigated. The first work was adsorption in a batch system of standard AFB1 solution on adsorbents. Some factors such as contact time, concentration of AFB1 and particle size of adsorbent were evaluated. The amount of AFB1 adsorbed was calculated based on the difference of AFB1 concentration before and after adsorption determined by high performance liquid chromatography (HPLC) method. Adsorption of AFB1 in corn sample was emphasized by mixing aqueous suspension of sample with adsorbent. Concentration of AFB1 in suspension was analyzed by enzyme-linked immuno-sorbent assay (ELISA) method. Result shows that adsorption of AFB1 on adsorbents of natural zeolite and bentonite is very fast. Within 15 min 99% of AFB1 (200 ng/mL) has been adsorbed by 25 mg of bentonite and 96% by zeolite. The particle size higher than 200 mesh did not give significant effect on the AFB1 adsorption capability. Effectiveness of zeolite in adsorbing AFB1 is lower than that of bentonite. Capability in reducing AFB1 contamination in corn samples (kernel and meal) for both adsorbents is lower than that in standard solution.

VALIDATION OF PCR-RFLP TESTING METHOD TO DETECT PORCINE CONTAMINATION IN CHICKEN NUGGET Tri Joko Raharjo; Winda Cahyaningtyas; Surajiman Surajiman; Istini Istini; Deni Pranowo
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

PCR-RFLP technique to detect porcine contamination in chicken nugget has been developed and validated in this research. Various concentrations of pork were fortified during preparation of the nugget. DNA was then isolated from the nugget followed by PCR employed primers which targeted a 359 bp cytB gene fragment of mitochondrial DNA. For RFLP, the PCR product was digested by means of BamHI and BseDI enzymes. Cutting DNA fragments from nugget containing pork using BseDI enzyme produced DNA fragment with size 228 and 131 bp, while cutting with BamHI enzyme produce DNA fragments with sizes 244 and 115 bp. All of these fragments were not present in RFLP analysis of pork-free nugget. The method shows good specificity and precision and could detect porcine contamination in the nugget up to 5%. The method has been applied to test commercial nugget. Four brand of Halal-labeled commercial nugget as well as four brand of non labeled one gave negative porcine contamination.

SYNTHESIS OF 4-(4-METHOXY-PHENYL)-3-BUTENE-2-ON AND THE ACTIVITY TEST AS A FRUIT FLIES ATRACTANT Deni Pranowo; Edhi Martono; Suputa Suputa; Muchalal Muchalal; Tutik D. Wahyuningsih; M. Yusuf Afandi
Indonesian Journal of Chemistry Vol 8, No 2 (2008)
Publisher : Universitas Gadjah Mada

4-(4-methoxyphenyl)-3-buten-2-on has been synthesized from p-anisaldehyde and acetone via aldol condensation. The reaction was performed at room temperature under basic condition for 12 hours to give brown solid of product (m.p 64-65 oC) in 66.19 % yield. p-anisaldehyde itself was produced from oxidation of anetol major component of anise oil by the use of potassium permanganate as a oxidator. The structure of the products was analyzed by FTIR, 1H NMR and GC-MS. Activity test of 4-(4-methoxyphenyl)-3-buten-2-on as an attractant was carried out in Sleman with methyl eugenol as a reference. The result showed that 4-(4-methoxyphenyl)-3-buten-2-on was inactive compound as a fruit flies attractant and some of fruit flies, i.e. Bactrocera papayae, B. carambolae, B. umbrosa and B. abdolonginqua was found on the test area.

THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM Tri Joko Raharjo; Rusmiati Suprihatin; Deni Pranowo
Indonesian Journal of Chemistry Vol 7, No 3 (2007)
Publisher : Universitas Gadjah Mada

A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (<10%) acetonitrile, methanol and ethanol followed by SDS-PAGE analysis of the supernatant in order to investigate the protein profile. There were no linear relationship between solvent concentration and the number of protein bands. However, an optimal condition of precipitation was found which is by methanol 0.1%.

CYTOTOXICITY OF Phaleria macrocarpa (Scheff.) Boerl. FRUIT MEAT AND SEED ETHANOL EXTRACT TO MONONUCLEAR PERIFER NORMAL CELL OF HUMAN BODY Endang Astuti; Deni Pranowo; Santi Dwi Puspitasari
Indonesian Journal of Chemistry Vol 6, No 2 (2006)
Publisher : Universitas Gadjah Mada

There were many research on Phaleria macrocarpa (Scheff.) Boerl. fruit for its activity as possible anticancer. However, there wasn't investigation that P. macrocarpa seed and fruit meat ethanol extract effect to normal cell. The research was conducted to identify the ethanol extract of P. macrocarpa for cytotoxic activity against mononuclear perifer normal cell of human blood. The research comprised several sections including P. macrocarpa seed and fruit meat maceration with ethanol, and the cytotoxic activity test against mononuclear normal cell. The results showed that the ethanol extract of P. macrocarpa fruit meat and seed was slightly toxic against mononuclear normal cell with the LC50 of 3817.54 μg/mL and 1349.29 μg/mL respectively. Tamoxifen and 5-fluorourasil, anticancer drugs were extremely toxic against mononuclear normal cell giving LC50 of 3.52 μg/mL and 4.05 μg/mL. The ANOVA f-test (P<0,05) showed that seed of ethanol has higher cytotoxic activity than fruit meat extract. Extract no cytotoxic activity difference between tamoxifen and 5-fluorourasil.

SYNTHESIS OF 4-(3,4-DIMETOXY-PHENYL)-3-BUTENE-2-ON AND ACTIVITY IT’S TEST AS A FRUIT FLIES ATRACTANT Deni Pranowo; Suputa Suputa; Tutik Dwi Wahyuningsih
Indonesian Journal of Chemistry Vol 6, No 1 (2006)
Publisher : Universitas Gadjah Mada

4-(3,4-dimethoxyphenyl)-3-buten-2-on has been synthesized from veratraldehyde and acetone via aldol condensation. The reaction was performed at room temperature under basic condition for 24 hours to give brown solid of product (m.p 64-6 5oC) in 73.7% yield. Veratraldehyde itself was produced from alkylation of vanillin by the use of dimethylsulfate. The structure of the products was analyzed by FTIR, 1H NMR and GC-MS. Activity test of 4-(3,4-dimethoxyphenyl)-3-buten-2-on as an attractant was carried out in Sleman, Bantul and Kulon Progo with methyl eugenol as a reference. The result showed that 4-(3,4-dimethoxyphenyl)-3-buten-2-on was inactive compound as a fruit flies attractant and some of fruit flies, i.e. Bactrocera papayae, B. carambolae, B. umbrosa and B. abdolonginqua was found on the test area.

CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL Endang Astuti; Sabirin Matsjeh; Winarto Haryadi; Deni Pranowo; Nuning Sri Mulatsih
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantz's supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.

Co-Authors Ahmad Taufiq Arminuddin Ali Agus Amri Setyawati Ebrahim Razzazi-Fazeli Edhi Martono Edhi Martono Endang Astuti Indriana Kartini Istini Istini M Muchalal M. Yusuf Afandi Muchalal Muchalal Nuning Sri Mulatsih Nuryono Nuryono Rahmi Fitrah Rusmiati Suprihatin Sabirin Matsjeh Santi Dwi Puspitasari Sri Wedhastri Suputa Suputa Surajiman Surajiman Tri Joko Raharjo Tutik D. Wahyuningsih Tutik Dwi Wahyuningsih Winarto Haryadi Winda Cahyaningtyas Y.M.S. Maryudhani Yunianta Yunianta

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