Indonesian Journal of Chemistry
Vol 7, No 3 (2007)

THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM

Tri Joko Raharjo (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta 55281)
Rusmiati Suprihatin (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta 55281)
Deni Pranowo (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta 55281)

Article Info

Publish Date
20 Jun 2010

Abstract

A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (<10%) acetonitrile, methanol and ethanol followed by SDS-PAGE analysis of the supernatant in order to investigate the protein profile. There were no linear relationship between solvent concentration and the number of protein bands. However, an optimal condition of precipitation was found which is by methanol 0.1%.

Copyrights © 2007




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Full PDF
280.948 KB
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Indonesian Journal of Chemistry
Website

Abbrev

ijc

Publisher

Universitas Gadjah Mada

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...