Indonesian Journal of Chemistry
Vol 9, No 2 (2009)

ANTI-MALARIAL COMPOUND FROM THE STEM BARK OF Erythrina variegata

Tati Herlina (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia)
Unang Supratman (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia)
M. S. Soedjanaatmadja (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia)
Anas Subarnas (Faculty of Pharmacy, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia)
Supriyatna Sutardjo (Faculty of Pharmacy, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia)
Noor Rain Abdullah (Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang 50588,Kuala Lumpur, Malaysia)
Hideo Hayashi (Laboratory of Natural Products Chemistry, Division of Applied Biological Chemistry, Graduated School of Agriculture and Life Sciences,Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan)



Article Info

Publish Date
22 Jun 2010

Abstract

During the course of our continuing search for novel anti-malarial compounds from Indonesian plants, the methanol extract of the bark of E. variegata showed significant anti-malarial activity toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The methanol extract of the bark of E. variegata  was separated by using bioassay-guide fractionation. The ethyl acetate fraction showed the most activity, exhibiting equipotency against both strains of parasite with IC50 of 23.8 µg/mL against 3D7 and 9.3 µg/mL against K1. Furthermore, by using the anti-malarial activity to follow separation, the ethyl acetate fraction was separated by combination of column chromatography to yield an active compound. The chemical structure of active compound was determined on the basis of spectroscopic evidences and comparison with those previously reported and identified as an isoflavonoid, warangalone. The warangalone showed anti-malarial activity against both strains of parasite used with IC50 of 4.8 µg/mL against 3D7 and 3.7 µg/mL against K1.

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Journal Info

Abbrev

ijc

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...