Indonesian Journal of Chemistry
Vol 8, No 1 (2008)

FOUR-FACTOR RESPONSE SURFACE OPTIMIZATION OF THE ENZYMATIC SYNTHESIS OF WAX ESTER FROM PALM KERNEL OIL

Erin Ryantin Gunawan (Department of Chemistry, FMIPA Universitas Mataram, Jl. Majapahit No. 62 Mataram 83125)
Dedy Suhendra (Department of Chemistry, FMIPA Universitas Mataram, Jl. Majapahit No. 62 Mataram 83125)

Article Info

Publish Date
17 Jun 2010

Abstract

The synthesis of wax ester using refined, bleached and deodorized (RBD) palm kernel oil (PKO) and oley alcohol catalyzed by Lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-factor central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (5-20 h), temperature (20-50 oC), amount of enzyme (0.1-0.2 g) and substrate molar ratio (palm kernel oil to oleyl alcohol, 1:1-1:5) on the percentage yield of wax esters. The optimum condition conditions derived via RSM were reaction time 8.46 h, temperature 44.4 oC, amount of enzyme 0.182 g, substrate molar ratio 1 to 3.7. The actual experimental yield was 92.9 % under optimum condition, which good accordance to the maximum predicted value of 92.4 %.

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Journal Info
Indonesian Journal of Chemistry
Website

Abbrev

ijc

Publisher

Universitas Gadjah Mada

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...