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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 13, No 1 (2018): May 2018" : 8 Documents clear
Bacterial Diversity of a Microbial Mat from Hot Spring at Wartawan Beach, Lampung and Its Potential as a Source of Hydrogenases Gintung Patantis; Ekowati Chasanah; Yusro Nuri Fawzya; He Pe Qing; Zhang Xue Lei
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.323

Abstract

Biohydrogen produced from thermophilic hydrogenases is an ideal and clean energy sources. As the biggest tectonic area in the world, Indonesia is potential for thermophile isolation. The aims of this study were to analyze the bacterial diversity of a microbial mat from hot spring at Wartawan beach, Lampung and to analyze the potency of microbial mat for hydrogenases, using clone library method. The diversity of 16S rRNA showed that the microbial mat sample contained 9 phyla of bacteria, and dominated by Cyanobacteria and Proteobacteria. These phyla indicate that the bacterial community of the microbial mat consisted of phototrophic and heterotrophic groups. In addition, a microbial mat of Wartawan beach environment might be influenced by marine environment and hydrothermal vent which was indicated by detection of both associated bacteria. The diversity of hydrogenase genes using NiFe hydrogenase (NiFe) and FeFe hydrogenase (FeFe) genes showed that Cyanobacteria was specifically related to NiFe, while Firmicutes was associated with FeFe. Proteobacteria and Bacteroidetes, however, were detected for both genes. The detected hydrogenase genes indicate that the microbial mat from hot spring at Wartawan beach is a promising source for hydrogenases isolation and further applications for biohydrogen production as a renewable energy. 
Antioxidant and Ace Inhibitor Potential of Stripe Trevally Fish (Selaroides leptolepis) Hydrolysate Reinal Putalan; Ifah Munifah; Tati Nurhayati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.319

Abstract

This study aimed to investigate the potency of fish protein hydrolysates (FPH) of stripe trevally fish (Selaroides leptolepis) as antioxidant and ACE inhibitor. The FPH was produced through enzymatically hydrolysis using protease produced by Bacillus licheniformis, a collection of Research and Development Center for Marine and Fisheries Product Processing and Biotechnology (RDCMFPPB). The FPH was fractionated using ultrafiltration membranes with molecular weight cut off (MWCO) of 10, 5 and 3 kDa. The hydrolysis degree, protein content, peptide content, antioxidant activity and Angiotensin-converting enzyme (ACE) inhibitor were observed. The result showed that FPH had maximum hydrolysis degree of 63.91% reached after 6 hours hydrolysis with protein content of 27.43 mg/mL and peptide content of 223.32 mg/mL. That FPH showed antioxidant activity (IC50) of 1941.06 ppm and ACE inhibitor of 87.82% at test concentration of 10 mg/mL. In the ultrafiltration step, the higher molecular cut off used, the higher protein content and peptide content. The lower molecular weight of the hydrolysate, the better antioxidant  and ACE inhibitor activity. The best fractionation that produce potential product to be used as anti-hypertension was in mixture peptides between 3-5 kDa. The IC50 antioxidant activity was 1336.96 ppm and percentage of ACE inhibitor was 97.15% % (with a concentration of 10 mg/mL). From the results, protein hydrolysate of stripe trevally fish produced by enzymatic hydrolysis using local protease was potential as a functional ingredient, particularly as antihypertensive agent.
CARAGEENAN OLIGOSACCHARIDES : BIOLOGICAL ACTIVITY AND ITS DEVELOPMENT OPPORTUNITIES IN INDONESIA Subaryono, Subaryono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.347

Abstract

Oligosaccharides from hydrocolloids especially carrageen now get a lot of attention because of the abundant raw material availability and various biological activities so it is potentially used in various fields both in the field of food and non food. Like oligosaccharides of terrestrial materials, carageenan oligosaccharides have many biological activities such as antibacterial, antiviral, antioxidant, immunomodulatory, antiinflamatory, anticancer, and other biological activity. Oligosaccharide carrageen can be produced either physically, chemically or enzymatically. This paper reviews articles on oligosaccharide carageenan, how it is produced, biological activity and development opportunities in Indonesia.
Back Cover Squalen Bulletin Vol. 13 No. 1 Tahun 2018 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.349

Abstract

Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts Nurrahmi Dewi Fajarningsih; Ifah Munifah; Dewi Seswita Zilda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.294

Abstract

Marine environment continuously produces pharmacologically active compounds. To screen marine natural products as a source of potential antibiotics, it is very important to select an accurate and efficient antibacterial assay. This study aims to find out the best assay for screening of antibacterial activity of marine invertebrate extracts by comparing the performance of 3 methods, i.e. the colorimetric resazurin microtiter assay (REMA), disc diffusion assay and spectrophotometric microdilution assay (SMA). Five marine invertebrate extracts, i.e. Stylissa sp., Theonella sp., Lobophytum sp., Sarcophyton sp., and Aaptos sp., were tested using those 3 different methods. The best method obtained was then further tested for its performance to screen 126 marine invertebrate extracts against 2 bacterial strains i.e. Escherichia coli (ATCC®25922TM) and Staphylococcus aureus (ATCC®25923TM). The study showed that, resazurin microtiter assay (REMA) was simpler, produced quicker result and able to generate reliable data for antimicrobial activity screening compared to the other methods. Moreover, REMA assay is regarded as a suitable assay platform to be implemented for screening of marine invertebrate extracts antibacterial activity since it requires only a small amount of extracts. Based on the MIC values, amongst 126 marine invertebrate extracts screened, 59; 36; 25 and 6 extracts tested against E. coli and 69; 36; 18; and 3 extracts tested against S. aureus were respectively categorized as not active, moderately potential, potential and very potential sample worth to be analyzed further. The ability of the REMA to generate accurate MIC value, which is comparable to the existing antibiotic drug MIC value, will empower researchers to decide whether the extracts worth to be examined further or not. 
Amino Acid and Fatty Acid Compositions of Sea Cucumber Stichopus Vastus from Salemo Island Waters, Indonesia Abdullah Rasyid
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.163 KB) | DOI: 10.15578/squalen.v13i1.285

Abstract

Coastal waters of Indonesia have considerable biodiversity of sea cucumbers. In the present study the amino acid and fatty acid contents in sea cucumber Stichopus vastus collected from Salemo Island waters Indonesia were determined. Results showed that all essential and non-essential amino acids were found in S. vastus. The major essential amino acid content was arginine (28651.62 mg/Kg). Whereas the major non-essential amino acid content was glycine (60907.24 mg/Kg). The total fatty acids were determined in which finding suggested that saturated fatty acid was more than polyunsaturated fatty acid and monounsaturated fatty acid. The higher saturated fatty acid, polyunsaturated fatty acid and monounsaturated fatty acid were palmitic acid (0.07%), arachidonic acid (0.13%) and palmitoleic acid (0.03%) respectively.  
Front Cover Squalen Bulletin Vol. 13 No. 1 Tahun 2018 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.95 KB) | DOI: 10.15578/squalen.v13i1.348

Abstract

Preface Squalen Bulletin Vol. 13 No. 1 Tahun 2018 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.568 KB) | DOI: 10.15578/squalen.v13i1.350

Abstract

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