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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 18 Documents
 1   2 
Search results for , issue "Vol 11, No 1 (2007): Annales Bogorienses" : 18 Documents clear
Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Sukara, Endang
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/21

Abstract

food security is  one of the essential needs which can never be  ignored by any society,  including in particular Indonesia. as the world population is expected to rise to around  10 billion marked by 2025. The problem  is that the agriculture in developed and developing countries that  follow the path of developed countries experiences create · a  range of  serious environmental degradation. An  intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources  for the agricultural purposes are not sustainable practice  .  In  consequences,  degradation  of ecosystem,  oil  erosion.  and  low  productivity  of land,  poor water quality and lost of biological  diversity  (including microbial  and  genetic  resources) occurred  at alarming  stale. Meanwhile,  the efforts  in  addressing  the above  situation  is  traditionally  focused on plant phenotypes (breeding. plant genetic engineering etc .) but the important role of microbial communities that interact with plants to influence plant health and productivity has been  largely ignored. The  interaction of microbes with plants and  its  relevant to soil  fertility and productivity, the bio-control properties of microbes and challenges toward sustainable agriculture is highlighted.
Construction of Binary Vector With Wound Inducible Promoter for Hbsag Expression: Development of Plant-Based Edible Hepatitis B Vaccine from Indonesian Isolate Suhandono, Sony; Rachman, Ernawati A.Giri; Zainuddin, Ima M.; Utari, Putri Dwi; Supraba, Apsari; Estiati, Amy
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/22

Abstract

Hepatitis B is a serious infectious disease In the third world countries including Indonesia . Vaccination is  the most effective way to prevent the spread of the disease;  therefore the demand for HBV vaccine is high.  In order to produce more vaccine at lower cost, transgenic plant can be chosen to express the vaccine with the above criteria. Several researches were successfully producing transgenic plants expressing HBsAg that formed virus-like particles and  induced  immune response  in  human.  However. HBsAg expression  in  transgenic plant needs to be  improved especially on gene  expression control system. Here, we describe the construction of HBsAg .  structural gene under the control of wound  inducible promoter, MeEFl promoter from  manihot esculenta Crantz. The HBsAg gene was amplified using PCR from HBV genome isolated from an  Indonesian patient. The gene was subsequently fused with VSPaS signal peptide, which targeted the reticulum endoplasm of plant cell . The construct was cloned into binary expression vector for Agrobacterium plant Transformation  in  near future.Keywords: HBsAg. VSPaS signal peptide and MeEFI promoter
Agrobacterium-Mediated Transformation of Javanica Rice Plants With A Cry1b Gene Under The Control of Wound-Inducible Gene Promoter Estiati, Amy; Rahmawati, Syamsidah; Astuti, Dwi; Loedin, Inez H.S
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/18

Abstract

A  cry1B synthetic gene of Bacillus thuringiensis has been used  for  the transformation of the javanica rice plants  cv.  Rojolele  confer resistance  to  an  important  pest,  yellow  stem  borer  (Scirpophaga  incertulas). Embryogenic callus were co-cultivated with  the EHA 105 strain of Agrobacterium tumefaciens harbouring binary vector CAMB  IA 130 I containing cry  / B gene under  the control of wound inducible gene promoter  (mpi), hygromycin resistance gene (hpt)  as a selectable marker and intron-containing β-glucuronidase (gus - intron) gene al) a reporter gene  driven  by  CaMV35S  promoter.  Previously.  our  histochemical  assay  and  P  R  analyses.  had  proved  th integration of  cry1B gene  into  the genome of rice plants at first generation .  However,  the existence of the gene should remain  table  throughout generation.  In  this study,  the presence of the cry1B transgene in  rice  transgenic plants at  second generation was confirmed by Polymerase Chain Reaction (PCR) .  Insertion of  the cry1B gene in the genome of PCR positive plants was  verified by Southern blot analysis is and showed that integration of cry1  B  into the genomic DNA of  javanica rice plants cv. Rojolele.  An effective resistance of transgenic  plants against  stem borer was verified in  bioassays.
Motif Ii of Japanese Encephalitis Vius Ns3 Pro is Not Essential for Rna Binding Activit Utama, Andi; Shimizu, Hiroyuki
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2930.744 KB) | DOI: 10.1234/19

Abstract

The role of motif n  (DEAR AsPw-GlulK,,-Ala.,.7-Hi  1~~  )  of Japanese encephalitis  virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to  the motif and  the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL2l(DE3) pLysS and purified by metal affinity resin. Asp-285 - and Glu-286 was respectively substituted with Ala, AJa-287 was replaced by Cys.,Gly.  or ser. His-288 was mutated to other 19 amino acids. In total. 24 mutant proteins were produced and analyzed. AL results, all mutants showed quite similar RNA binding activity.  Indicating that motif II of JEY NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C virus c (Hey) NS3 protein, suggesting the similar structure of NS3 protein in flavivirus .Keywords: Japanese encephalitis, R.binding, motif
Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV.Rojolele And Japonica Rice CV. Gajahmungkur Nugroho, Satya; Loedin, Inez HS; Ouwerkerk, Pieter BF
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2649.41 KB) | DOI: 10.1234/20

Abstract

Retrotransposons arc  mobile  genetic  elements  that  transpose  via  an  RNA intermediate  that  is  reverse transcribed  before  integration  into  a  new  location  within  the  host  genome. They  are  ubiquitous  in  eukaryotic organism.  and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants .Tos17 is a  rice  endogenous  retrotransposon  on  that  has  been  studied  thoroughly. Tos17 has  been  shown  to  be  an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonicarice cv  Nipponbare.  In  lavanica rice however, the presence and activity of  Tos17 has not been described thus far. while in some Indica rice Tos17 has  been found  to be  inactive. Javanica rice, also known as tropical  Japonica rice,has many cultivars which may serve as pontetial genetic resources of great interest for breeding programmes. Here  , the presence and activities of retrotransposon Tos17 in  Javanica rice  cv Rojolele was described and compared  to those of Japonica rice and Japonica rice cv Gajahmungkur We  identified  five and theree copies of  Tos17 in Rojolele and Gajahmungkur, respeclively, with different activities.Keywords: Oryza  sativa, rice, javanica, retrotransposon, Tos17
Editorial Boards AB Vol 11 No 1 (2007) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.21 KB) | DOI: 10.1234/124

Abstract

Front Cover AB Vol 11 No 1 (2007) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.453 KB) | DOI: 10.1234/123

Abstract

Guide for Authors AB Vol 11 No 1 (2007) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/126

Abstract

Back Cover AB Vol 11 No 1 (2007) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.001 KB) | DOI: 10.1234/127

Abstract

Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Endang Sukara
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4484.648 KB) | DOI: 10.14203/ann.bogor.2007.v11.n1.31-39

Abstract

food security is  one of the essential needs which can never be  ignored by any society,  including in particular Indonesia. as the world population is expected to rise to around  10 billion marked by 2025. The problem  is that the agriculture in developed and developing countries that  follow the path of developed countries experiences create · a  range of  serious environmental degradation. An  intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources  for the agricultural purposes are not sustainable practice  .  In  consequences,  degradation  of ecosystem,  oil  erosion.  and  low  productivity  of land,  poor water quality and lost of biological  diversity  (including microbial  and  genetic  resources) occurred  at alarming  stale. Meanwhile,  the efforts  in  addressing  the above  situation  is  traditionally  focused on plant phenotypes (breeding. plant genetic engineering etc .) but the important role of microbial communities that interact with plants to influence plant health and productivity has been  largely ignored. The  interaction of microbes with plants and  its  relevant to soil  fertility and productivity, the bio-control properties of microbes and challenges toward sustainable agriculture is highlighted.

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