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Jurnal Ilmu Alam dan Lingkungan

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Published by Universitas Hasanuddin

ISSN : 20864604 EISSN : 25498819 DOI : -

Core Subject : Health, Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Veterinary

Jurnal yang memuat hasil - hasil penelitian terkait ilmu alam dan lingkungan termasuk review meliputi lingkungan, kelautan, konservasi, mikrobiologi, bioaktif, dan yang relevan.

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Search results for , issue "Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan" : 10 Documents clear

PRODUKSI DAN KARAKTERISASI ENZIM KITINASE DARI BAKTERI KITINOLITIK ASAL KERANG Anadara granosa Nur Haedar; Fahruddin Fahruddin; Wilda Aryanti; Hasnah Natsir
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

This research aims to investigate the maximal generation time and the characterization of chitinase enzyme of Chitinolytic bacteria from Anadara granosa shell in various pH, temperature and substrate concentration. The result of the maximal time of Chitinolytic bacteria was showed at 120 hours. The result of Chitinolytic bacteria characteristic in various pH shows that the highest activity of chitinase is on pH 6 and in various temperature shows that the highest activity at 350C while in various substrate concentration shows that the highest activity value of chitinase is on substrate concentration 0.5%. Keywords: Characterization, Chitinolytics Bacteria, Chitinase Enzyme

Struktur Komunitas Padang Lamun di Perairan Kepulauan Waisai Kabupaten Raja Ampat Papua Barat Muh Haidir Ansal
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

The research about Community structure of seagrass was conducted in the waters of Waisai, South Waigeo District, Raja Ampat, West Papua. The aim of this study was to determine the community structure of seagrass beds found in the waters of the Waisai archipelago, Raja Ampat, West Papua using quadrant transect method. Data of community structure were obtained at 3 stations that were station I in the countryside of Waisai (Post AL), station II in the Resort Waiwo (Waiwo Dive Center) and Station III in Saporkren village near residential areas. The results of this study discovered seven species of seagrass; Enhalus acoroides, Halophila decipiens, H. ovalis, Thalassia hemprichii, Cymodocea rotundata, Halodule pinifolia, Syringodium isoetifolium in all stations. Cymodocea rotundata had the highest importance value index (IV) which amounted to 79.22%, 161.15% and 134.68% in each station, respectively. While Syringodium isoetifolium had the lowest importance value index, respectively 00,00%, 6.91% and 7.80% in each station. Seagrass of Waisai waters had clumped distribution patterns. The seagrass Cymodocea rotundata is the most dominant seagrass species and influential in the community and the results of environmental parameters, pH, temperature, salinity, substrate, measured in research location are still feasible and great for the growth of seagra

Keragaman Jenis Tumbuhan Obat Indigenous Di Sulawesi Selatan Elis Tambaru
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Penelitian keragaman jenis tumbuhan obat indigenous berasal dari tumbuhan liar di alam. Penelitian ini bertujuan untuk mengidentifkasi keragaman jenis-jenis tumbuhan indigenous, organ tumbuhan yang digunakan, dan cara pengolahannya untuk penanggulangan beberapa macam penyakit pada masyarakat di Sulawesi Selatan. Metode penelitian ini adalah deskriptif yang bersifat eksploratif. Pengumpulan data kualitatif dilakukan dengan metode jelajah Cruise Method. Hasil penelitian menunjukkan, bahwa tumbuhan indigenous berkhasiat obat ditemukan sebanyak 9 (sembilan) jenis yaitu: bandotan Ageratum conyzoides L.; kirinyu Eupatorium odoratum L.; insulin Tithonia diversifolia (Hemsl.) A. Gray.; hiptis Hyptis capitata Mart. & Gal.; patikan kebo Euphorbia hirta L.; tembelekan Lantana camara L.; senggani Melastoma malabathricum L.; sirih-sirihan Peperomia pellucida (L.) Kunth, dan ketepeng cina Cassia alata L. Organ tumbuhan yang banyak dimanfaatkan sebagai bahan baku obat yaitu: organ daun dan proses pengolahan dengan cara direbus. Kata kunci: keragaman , tumbuhan obat, indigenous

Aktivitas Antimikroba Ekstrak Dietil Eter Rimpang Lempuyang Wangi (Zingiber aromaticum Vahl.) Terhadap Bakteri Patogen Secara Klt-Bioautografi Zaraswati Dwyana
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Antimicrobial Activity of Dietil Eter extract of Lempuyang Wangi Rimpang (Zingiber aromaticum Vahl.) to pathogen bacterial through TLC-Bioautography. Research has done by screening test using Streptococcus mutans, Vibrio sp, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi, Escherichia coli and Candida albicans by methanol extract, diethyl ether extract and nbuthanol extract which were use 1 mg/ml. Result which obtained exhibit diethyl ether extract inhibit the growth of Bacillus subtilis, Salmonella typhi, Staphylococcus epidermidis, and Vibrio sp. Antimicrobial potential assay of diethyl ether extract of Zingiber aromaticum Vahl. has done by liquid dilution method to get the MIC value in concentration of 0,05 %, 0,1 %, 0,2 %, 0,4%, and 0,8 % which continued by smearing on solidified medium to get the MLC value. The result shown that MKC could not be determined due to turbidity of the test solution mean while the MLC of the extract as much as 0,2 % on Bacillus subtilis and Salmonella typhi and 0,4 % on Staphylococcus epidermidis and Vibrio sp. TLC-bioautography test has done to get the compound which had antimicrobial activity. The best result was obtained from separation through TLCbioautography by means of eluent n-hexan : ethyl acetate (8 : 2). TLC-bioautography test result shown that the spot in Rf 0,07 has antimicrobial activity on Staphylococcus epidermidis, Rf 0,2 has antimicrobial activity on Vibrio sp., Rf 0,07, 0,45, 0,56 and 0,69 has antimicrobial activity on Bacillus subtilis, and Rf 0,2 has antimicrobial activity on Salmonella typhi. Identification result of the chemical component shown that the active compound which given positif result on spot viewer were in Rf 0,07; 0,2; 0,45; 0,56; and 0,69.

Respon Pertumbuhan Tanaman Jengger Ayam Merah Celosia plumosa (Voss) Burv. Pada Tanah Tercemar Logam Berat Kadmium (Cd) Juhriah Juhriah; Sri Suhadiyah; Reski Mandasari
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Research on the growth response of Red Cockscomb Plant Celosia plumosa (Voss) Burv. on contaminated soils heavy metal Cadmium (Cd) has been carried out from March to July 2016 in Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. The aims of this research is to find out the growth response of red Cockscomb Celosia plumosa (Voss) Burv. on the ground of cadmium heavy metals (Cd) and its potential as phytoremediation agent of Cd metal on soil. The study was arranged in a Randomized Block Design, 4 treatments (source of sewage sludge land, garden soils, rice field and landfill or final waste disposal) and 3 groups. The cadmium content analysis was performed at Centerfor Health Laboratory of Makassar, South Sulawesi, using Atomic Absorption Spectrophotometer (AAS). The data were tested by F test, the different significant result was followed by the Smallest Differential Test (SDT). The results of the analysis showed that the growth of Red Cockscomb Plant Celosia plumosa (Voss) Burv. plants able to live better (higher plants, more leaves and wet or dry weight higher) on the land from landfills (final waste disposal ) is compared to the other three sources of land.

Analisa Kadar Kalsium (Ca) Pada Daun Kelor (Moringa oleifera) Mardiah Mardiah
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Moringa leaves (Moringa oleifera) is one part of the Moringa plant which can be consumed as a vegetable and mineral content in the form of calcium which is quite a lot. Calcium is a mineral needed by the human body, it has an important role in various stages of metabolism. Calcium is useful to prevent osteoporosis, blood clotting and building bones stronger teeth. Lack of calcium in the body can lead to calcium deficiency, which affects the various complaints in the bones, teeth, blood, nerves, and metabolism. The purpose of this study to analyze the levels of calcium (Ca) in the leaves of Moringa (Moringa oleifera). Type of research is an observational laboratory with Quantitative Analysis technique. The sample used in this study were 5 samples. Sampling technique is an accidental sampling. Furthermore, each sample in dry digestion is then examined at an atomic absorption spectrophotometer, at a wavelength of 422.7 nm. Based on the research results obtained by the levels of calcium in the leaves of Moringa is 7059.2 mg/L, 4652.5 mg/L, 3.180 mg/L, 2078.9 mg/L, and 9268.7 mg/L indicates that in the leaves of Moringa contains a lot of calcium. From this study, it can be concluded in the presence of calcium that can meet the needs of the mineral in the human body

Pengaruh Tegakan Lamtoro Gung Leucaena leucocephala L. Terhadap Kesuburan Tanah di Kawasan Hutan Ko’mara Kabupaten Takalar Budirman Bachtiar; Resti Ura’
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Lamtoro gung Leucaena leucocephala L. merupakan tanaman sela kerena mampu memelihara dan meningkatkan produktivitas tanah. Penelitian ini bertujuan mempelajari dan membandingkan sifat-sifat tanah dibawah tegakan lamtoro gung dengan semak belukar. Hasil penelitian menunjukkan bahwa kesuburan fisik atau kimia tanah (berat volume, volume total pori tanah, nilai kapasitas tukar kation, kandungan bahan organik, nitrogen, fosfor,kalium, kalsium dan magnesium) memperlihatkan nilai yang lebih baik di bawah tegakan lamtoro gung yang berumur 2 tahun dibanding pada sifat kesuburan tersebut pada semak belukar yang didominasi oleh semak dan rumputrumputan. Meningkatkan kesuburan tanah di bawah tegakan lamtoro gung diduga sebagai pengaruh dari kandungan bahann organik di bawah tegakan tersebut lebih tinggi (9,2%) dibanding dengan kandungan bahan organik pada tanah semak belukar (5,1%), dimana bahan organik ini merupakan sumber pokok beberapa unsur hara dalam tanah.

Bioaktivitas Ekstrak Daun Avicenia Marina Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Eva Johanes
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Avicenia marina yang dikenal dengan nama api-api, merupakan salah satu spesies mangrove, memiliki manfaat dalam bidang pangan dan kesehatan, yang berpotensi untuk dikembangkan. Penelitian ini bertujuan mengetahui aktivitas senyawa bioaktif yang terdapat pada ekstrak daun A. marina dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Sampel A. marina diperoleh dari Karst Ramang-Ramang Kabupaten Maros Propinsi Sulawesi Selatan. Uji antibakteri dilakukan di laboratorium mikrobiologi Departemen Biologi fakultas MIPA Unhas. Hasil uji antibakteri menunjukkan A. Marina, bersifat bakteriostatik terhadap bakteri Staphylococcus aureus

Ligasi Gen Rv 1980c Pengkode Protein MPT 64 KE pGEM-T Mycobacterium tuberculosis Sebagai Antigen untuk Immunodiagnostik Tuberkulosis Laten Rosana Agus; Fahruddin Fahruddin
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Penyakit tuberkulosis yang disebabkan oleh Mycobacterium tuberculosis merupakan penyakit infeksi penyebab kematian manusia terbesar di dunia. Tantangan utama dalam pengendalian TB adalah mendiagnosis secara cepat dan tepat penyakit TB khususnya TB laten. Deteksi TB laten tidak memiliki standar baku, namun saat ini dilakukan dengan uji tuberculin skin test (TST). Prinsip uji tuberkulin adalah timbulnya hipersensitivitas pada seseorang yang terinfeksi M. tuberculosis terhadap komponen tuberkulin yaitu purified protein derivative (PPD). PPD mengandung kurang lebih 200 antigen mikobakteri, sehingga uji tuberkulin mempunyai keterbatasan antara lain terjadi reaksi positif palsu karena adanya reaksi silang antara PPD dan antibodi yang dihasilkan oleh vaksinasi BCG atau infeksi dengan mikobakteria bukan TB. Dengan demikian, ketersediaan reagen diagnostik untuk TB sangat diperlukan yang secara ideal dapat mengidentifikasi individu terinfeksi baru dan laten dengan resiko tinggi untuk berkembang menjadi tuberkulosis aktif. Beberapa protein yang dikode pada daerah RD 2 Mycobacterium tuberculosis telah dikembangkan menjadi vaksin dan reagen diagnostik, antara lain protein MPT 64. Protein ini merupakan kandidat yang sesuai untuk pengujian diagnostik yang berbasis sel T. Protein ini dapat pula membedakan penderita TB aktif, TB laten dan orang sehat yang telah divaksin BCG. Tujuan penelitian ini adalah untuk meligasi gen MPT 64 Mycobacterium tuberculosis ke sel host Escherichia coli JM 109 sebagai imunodiagnostik TB laten. Metode yang digunakan adalah mengkultur M.tuberculosis dalam medium Lowenstein Jensen, mengisolasi DNA kromosom, mengamplifikasi gen Rv 1980c (MPT 64) dengan PCR. Selanjutnya meligasi gen tersebut ke vektor kloning dan transformasi ke sel host E.coli. Karakterisasi klon rekombinan dilakukan dengan PCR. Hasil penelitian diperoleh beberapa koloni putih dari pGEM-T-MPT64. Setelah dilakukan karakterisasi klon diperoleh bahwa DNA sisipan yang diligasi ke vektor pGEM-T dan transformasi ke sel host E.coli adalah benar MPT 64.

Induksi Kalus Pisang Barangan Merah Musa acuminata Colla dengan Kombinasi Hormon 2,4-D dan Bap Secara In Vitro Andi Ilham Latunra
Jurnal Ilmu Alam dan Lingkungan Vol. 8 No. 1 (2017): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Research on callus induction of red banana Musa acuminata Colla with combination hormone 2,4-D and BAP in vitro aims to find a combination of hormones BAP and 2,4D were effective in inducing callus red banana. This study was conducted from February until April 2016 at Tissue Culture Laboratory Research and Development Center of Biotechnology, Hasanuuddin University, Makassar. Callus was induced from leaf explants red banana from the in vitro culture of a 5 month old, the basic medium MS + 2,4-D + BAP. This designs uses in completely randomized design with 4 treatments with 3 repetitions. The combinations were : MS medium + 1 ppm 2,4-D + 4 ppm BAP (W1), MS medium + 2 ppm 2,4-D + 3 ppm BAP + (W2), MS medium +3 ppm 2,4 -D + 2 ppm BAP (W3) and MS medium + 1 ppm 2,4-D + 4 ppm BAP (W4). Parameters observate in this study were the emergence of callus and percentage appears callus, callus color, texture callus, and callus wet weight. The results showed that the combination of MS medium + 2 ppm 2,4D + 3 ppm BAP (W2) is the best medium to induce callus, marked by green callus, compact callus (friable), and the highest wet weight of callus 0.0203 g.

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Fahruddin

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jurnalalam84@gmail.com

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fahruddin65@gmail.com

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Kota makassar,

Sulawesi selatan

INDONESIA