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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 17 Documents
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Search results for , issue "Vol 14, No 2 (2022): August 2022" : 17 Documents clear
Electroencephalogram Detection for Insomnia Patients: A Preliminary Study Inosensia Lionetta Pricillia; Ahmad Azhari
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.36255

Abstract

Measurement of insomnia is currently generally carried out by medical practitioners by looking at the patient's condition accompanied by symptoms that refer to insomnia. In contrast, minimal quantitative measurements were found. This study proposes an alternative measurement with the acquisition of brainwave activity through electroencephalogram (EEG) in identifying sleep disorders. Insomnia is a common sleep disorder that can make it difficult to fall asleep difficult to stay asleep, or cause waking up too early and not being able to go back to sleep. Insomnia not only weakens energy levels and moods, but also a person's health, performance, and quality of life. This sleep disorder appears due to several factors, such as anxiety, stress, depression, bipolar disorder, or trauma. Photic stimulation is given as an attempt to find a person's body's response to light. Late adolescents with insomnia symptoms with an age range of 17-25 years were included as respondents, had previously been given a simulation test related to the treatment of sleep disorders, and identified severe, moderate, and mild insomnia. Acquisition using Narosky Mindwave Mobile 2 with the electrode in forehead position, Fp1. This study compares several types of insomnia data acquisition from previous studies and obtains patterns of insomniacs based on photic stimulation.
Liver Histopathology of Rats Induced by High-Fat Feed After Giving Neem Leaf Ethanol Extract Sri Isdadiyanto; Anggih Retno Pratiwi; Siti Muflichatun Mardiati
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.34383

Abstract

Foods that contain high levels of fat can cause hyperlipidemia, which is one of the triggering factors for non-alcoholic fatty liver disease. Neem leaves contain flavonoids, alkaloids and tannins which have the ability to act as hepatoprotectors. This study aimed to determine the Liver Histopathology of Rats Induced by High-Fat Feed After Giving Neem Leaf Ethanol Extract. Twenty-four the male white rats (Rattus norvegicus L.) were divided into 6 treatment groups, namely: normal control (P0); negative control (P1: given high-fat diet); P2 treatment (P1+ 8 mg/200gBW simvastatin); and P1+ the dose of neem leaf ethanolic extract of  75; 100; and 125 mg/200gBW (P3; P4; and P5). Fixation process with 10% Neutral Formalin Buffer (NFB) solution. Liver histopathological preparations were made by paraffin method and Hematoxylin-Eosin staining, histopathological observations with a 400x magnification microscope. Liver histopathology was analyzed descriptively, homogeneous and normally distributed data of liver weight and hepatocyte diameter were analyzed statistically using ANOVA followed by Duncan's test with a significance level of 5% using SPSS 16.0 software. The results showed that the administration of ethanolic extract of neem leaves could improve the liver histology structure. From this study it was concluded that the ethanolic extract of neem leaves can be used as an alternative hepatoprotector.
Application Of Unmanned Aerial Vehicle (UAV) Remote Sensing Technology for Estimation of Tree Height in Heterogeneous Forest Francine Hematang; Agustinus Murdjoko; Hendri Hendri; Max Tokede
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35637

Abstract

Tree height is an important piece of information in forest management. Cost, time, and effort are some of the limiting factors in extracting tree height values on a large scale. The canopy height model approach through aerial photography using UAV can be used to quickly estimate the height of large-scale trees combined with field measurements. CHM analysis was carried out using spatial statistics to get the maximum tree height value based on the tree canopy. Evaluation of accuracy in the form of statistical tests is used to assess the level of accuracy of the estimation. Photogrammetry results show that the obtained CHM has a resolution of 11.8 cm/pixel with the results of the evaluation of tree height accuracy having an RMSE of 2.4 m, MAE 2.0 m, SDE 3.8 m. The chi-square statistical test shows that the results of the tree height estimation accept H0 and there is a strong relationship between the observed tree height and the estimation through linear regression with an R2 value of 0.67. The broad estimation of height shows that Mansinam Island has a tree height in the range of 7 – 66 m. The dominant tree height is in the 19-30 m class with the number of individuals reaching 1,877 trees. This study shows that CHM obtained from aerial photography using low-cost UAVs is still able to estimate tree height well. For future studies, it is necessary to use a ground control point (GCP) to increase the accuracy of the elevation model and orthophoto.
Water Management Practices and Environmental Attitudes of Riparian Communities in Sapangdaku River, Cebu Island, Philippines Joje Mar Perino Sanchez; Reginald Raymund A. Caturza; Marchee T. Picardal; Judelynn M. Librinca; Ruby L. Armada; Hedeliza A. Pineda; Marnan T. Libres; Ma. Lourdes B. Paloma; Sherry P. Ramayla; Jay P. Picardal
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.36185

Abstract

Understanding the social-ecological dynamics of freshwater ecosystem is critical for effective planning and sustainable use of the resource. The study aimed to determine the socio-demographic profile, anthropogenic activities, water utilization of the residents, and waste disposal practices along the Sapangdaku River. Here, we examined water utilization, waste disposal practices and their corresponding impact to health, and environmental attitudes of riverside dwellers (n=120) in Sapangdaku River by using a standardized social health and environmental attitudes survey, respectively. Results show that while river water is still largely used for bathing and backyard farming, its known function as (a) disposal area of human and animal waste, (b) quarry site for pebbles and sand and (c) laundry area significantly decreased in recent years. Responses revealed that occurrence of very common ailments such as cough, gastroenteric problems and skin problems are partly associated to poor river water quality. In terms of people’s environmental attitude, ecocentrism is both positively correlated to environmental movement activism (r=0.445, p=0.000) and human utilization of nature (r=-0.275, p=0.006), putting prime value on the sustainable use of river water that provides benefit to the community. Similarly, environmental movement activism and human utilization of nature (r=-0.327, p=0.001) indicates willingness to participate in environmental activities, hence supported by their decision not to use their environment unfavorably. These findings suggest that community’s concern for the river water resulted to increased participation in various strategies to better maximize the use of the river for various purposes. However, efficient management and restoration of river quality require a holistic view of the problem.
Antibacterial Activity of Melaleuca alternifolia Extract from Different Extraction Method Yuli Widiyastuti; Husnul Khotimah Matoha; Fitriana Fitriana
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.36223

Abstract

Melaleuca alternifolia (Tea tree/MA) is a powerful antimicrobial agent that could be one of the solutions to antimicrobial resistance. The important benefit of this plant comes from its volatile oil compounds named tea tree oil (TTO). On the other hand, studies related to the use of tea tree leaf extract as antimicrobial were still limited.  Therefore, an evaluation of the active compound content and antimicrobial activity of tea tree extract obtained from different extraction methods will be carried out. Thin-layer chromatography (TLC) was performed to analysis the chemical profile,  the antibacterial activity of the extracts was evaluated using the Kirby Bauer method against Staphylococcus aureus and Escherichia coli, whereas the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined by broth dilution method. Based on the chromatographic profile of the extract indicated that cardiac glycosides and terpenoids could be present in maceration and soxhletation extracts. This study suggests that maceration and soxhletation yielded different bioactive compounds from  MA. Extracts of MA from both method have an excellent activity to inhibit the S. aureus, and E. coli in a dose-dependent manner. Maceration extract of MA has a stronger effect against E. coli meanwhile the soxhletation extract of MA reveals to have stronger antimicrobial activity against S. aureus. The both extract even obtained from different extraction method yielded the same MIC and MBC values namely = 0.1875% against E. coli. In contrast MBC of S. aureus range from two to fourfold of the MIC, and the maceration seem to have the highest MBC value (MBC = 12%).
The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro Irma Suryani; Kartini Eriani; Suhartono Suhartono
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35722

Abstract

Physalis minimahas been used as herbal medicine because it is believed by the community to cure neurodegenerative and cardiovascular diseases. This study aims to determine the effectiveness of P.minima extract in increasing the proliferation of mesenchymal stem cells (MSC) from mouse (Musmusculus) bone marrow (BM). BM from the femur and tibia were isolated using a flushing method. BM-MSC primary culture was conducted in mesenPROÒ medium at 37ᵒC in a 5% CO2 incubator until it reached a 70% confluence.BM-MSCs were sub-cultured overnight in Dulbecco's Modified Eagle's Media (mDMEM). The mDMEM was replaced with a treatment medium on the second day of subculture. The treatment medium was changed every three days and evaluated under an inverted microscope by counting the number of cells at the beginning and the end of the incubation period. The proliferation rate is expressed as PDT, which was statistically analyzed using ANOVA at a significance level of 0.05% and followed-up with Duncan's test. Statistically, P. minima leaf extract could significantly reduce the PDT value. The optimum dose of P. minima leaf extract that can increase the proliferation of BM-MSC was 0.8 mg/ml. It is concluded that P. minima leaf extract was effective as an inducer of BM-MSC proliferation. The data obtained is the preliminary data on the use of P.minimaextracts in stem cell-based therapy. The results of this study provide important information in scientifically proving the potential of P. minima extract on stem cell proliferation.
The Effect Of Moringa Leaf Extract On Hyperglycemic Rat Liver Function Wulan Christijanti; Aditya Marianti; R. Susanti; Senda Kartika Rakainsa
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35431

Abstract

One of the management of diabetics is to maintain stable glucose levels. Often diabetes treatment combines chemical drugs with medicinal plants. People have consumed a lot of Moringa leaves which are believed to be able to maintain body condition. One of the benefits of Moringa leaves with phytochemical components in it is as a hepatoprotector. The aim of this study was to analyze the role of Moringa leaf extract on liver function parameters of hyperglycemic rats. There were 4 groups, normal control (K0), hyperglycemic rats with induction of alloxan 125 mg/kg bw and divided into three groups treated with moringa leaf extract at a dose of 0 mg (K1), 200 mg (T1) and 400 mg/kg bw (T2 ) for 21 days. The variables measured were aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, total cholesterol. Data were analyzed with Anova P0.05 and Least Significance Different (LSD) follow-up test. The results showed that the levels of AST and ALT were highest in the K1 group which then decreased significantly in the group that received Moringa extract (T1 and T2). Statistically there was a significant difference (p0.05) between the control and treatment groups. Cholesterol levels in the control group were significantly lower than the diabetes and treatment groups, but there was no significant difference between the treatment groups. The conclusion is that Moringa leaf extract has an effect on reducing liver enzyme levels and cholesterol in hyperglycemic rats. This research adds to the study of the hepatoprotective potential of Moringa leaf extract in hyperglycemic rats.
Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine Megananda Aprilia; Nintya Setiari; Yulita Nurchayati
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35703

Abstract

Potato has the potential for food diversification.The propagation method in a short period is needed. One of the methods used is plant tissue culture. Benzyl Amino Purine (BAP), determine the success of propagation by tissue culture method. This research aimed to study the use of different BAP concentrations for callus development from stem explants. The explants put on culture medium added by 0, 1, 2, 3 ppm BAP. The development of the stem explant observed every week for a month. The results showed that callus formed in all media. Without BAP treatment, callus were formed after 2 weeks and got browning, then stopped growing. Callus grew and showed differentiation by application of all the BAP concentration. Callus greowth was optimally at 2 ppm BAP treatment. The callus from 1 ppm BAP produced the most number of roots, shoots and leaves than another concentration.  This experiment showed that different BAP concentrations affected callus development of S. tuberosum from stem explant. The conclusion was callus growth has obtained by the treatment of 2 ppm BAP, while the development of callus has obtained on addition of 1 ppm BAP. The novelty of this research is the callus induction method from potato sprout stems grown from potato seeds with plant growth regulators Benzyl Amino Purine. Callus induction method from potato sprout stems grown from potato seeds can be a guide for embryogenic callus induction.
Microbiological and Biochemical Tests on Tempe Production Using Tempe Mold Innovation Siti Harnina Bintari; Dhimas Fajar Eka Purnama; Danang Dwi Saputro; Sunyoto Sunyoto; Pramesti Dewi; Ibnul Mubarok
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.37700

Abstract

Tempe is a popular fermented food in Indonesia, one of the important things in the process of making tempeh is the tempe incubation container. The purpose of this study was to prove the quality of tempe products made using the tempe mold innovation suitable for production, to analyze the comparison between the quality of tempe products made using a tempe incubation tool and without using the tool. Experimental research with Completely Randomized Design (CRD), applying a tempe incubator and without using a tool as a control. Test methods used include testing water content, hygiene, number of mold colonies and protein content. Based on the results of the study, tempe products made using a tempe printer contain a moisture content of 51.4 - 56.2%, protein content 17.92 - 18.58%, the number of mold colonies 3.65 x 105 untill 4.08 x 105 cell/gr and negative Escherichia coli. Based on the results of the study, it was concluded that the tempe products made using the tempe mold innovation tool had met the biological and chemical quality standards based on SNI 3144:2015 and had an overall quality test result that was superior to the control.
Carbon Stocks in the Conservation Area of PT. Pertamina (Persero) Fuel Terminal Boyolali Sunarno Sunarno; Rully Rahadian; Sri Widodo Agung Suedy; Bayu Pradika; Bima Adistya
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35074

Abstract

Flora biodiversity has been known to have a vital function in reducing carbon emissions in the air and contributing oxygen in significant levels to the environment. PT. Pertamina (Persero) Fuel Terminal Boyolali (FTB) has an important role in the conservation programs, protection of flora biodiversity, and environmental management. This study aims to analyze carbon stocks found in trees, plants cover soil, litter, necromas, and carbon in the soil and evaluate conservation of flora. This study uses a purposive random sampling method of carbon sources followed by the determination of carbon stocks in three conservation areas managed by PT. FTB, namely in the Wonopotro Forest, in the Pepe River watershed, and Pertamina's operational area. The samples from carbon sources include trees, litter, ground cover plants, necromas, and soil. The trees and necromas carbon sources sampling at each location was carried out at 2 stations with a size of 10 x 50 m2. Meanwhile, the sampling of litter, ground cover plants, and soil was carried out at 2 stations, each consisting of 3 plots. The results showed that the total carbon stock in the conservation areas was 1,688,0791 tons. The Pepe River watershed has the highest total carbon stock, which was 1,466,0928 tons/ha. The FTB and Wonopotro Forest, respectively, have about 180.7569 and 41.2294 tons/ha. The novelty of this research is the method of calculating carbon stocks in a conservation area that can be used as a database for assessing environmental management performance to the preservation of biological resources.

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