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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue "Vol 15, No 3 (2023): Inpress December 2023" : 2 Documents clear
The Growth of Tagetes patula Linn. and Its Ability to Reduce Cr(VI) with the Addition of Microbacterium sp. SpR3 Firdiana Novellasari; V. Irene Meitiniarti; Mahmoud F. Seleiman; Sri Kasmiyati
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): Inpress December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.44957

Abstract

Cr(VI) is a heavy metal that has the potential to become a soil pollutant and has an impact on organisms. The contamination caused by Cr(VI) could be alternatively treated with bioremediation techniques. The current study aimed to determine the most potential combination of Tagetes patula Linn. and Microbacterium sp. strain SpR3 for remediation of soil Cr(VI) contamination based on growth of T. patula. The application of SpR3 applied at the 1st day (T0) and 20th day (T20) with 10 g (M10), 30 g (M30), 50 g (M50) of bacterial inoculum to T. patula grown under Cr(VI; 100 mg/L). The results showed that T0M50 treatment resulted in the highest values of growth traits of T. patula grown under Cr(VI) metal stress. The highest BC value (0.36) was obtained from plants treated with T2M10 and T2M50, while the highest TF value (0.08) obtained from plants treated with T0M50. BC value 1 means that the combination of T. patula and SpR3 bacteria for heavy metal Cr(VI) can be classified as an excluder and the TF value 1 means that the combination can act as a phytostabilization in handling Cr(VI) contamination. In conclusion, the application of SpR3 using T0M50 can enhance the growth of Tagetes patula Linn. grown under Cr(VI) stress condition. The outcome of the study are expected to advancement in the application of rhizobacterial and plant combined system in the bioremediation of soil Cr(VI) contaminated.
Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer Dewi Indriyani Roslim; Herman Herman; Shalsadila Rahmadani Putri; Aries Tri Furqoni; Deanne Yoshe Fidela Budiono; Adeel Abdulkarim Fadhl Altuhaish; Wahyu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): Inpress December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.46937

Abstract

Maman is a traditional plant frequently used as food by people in Melayu Rokan of Riau Province. Morphological detection indicates that the scientific name of maman is Cleome gynandra L.  The objective of this study is to confirm the taxonomic status of maman from Riau basedon matK and trnL-trnL-trnF intergenic spacer (IGS) sequences. Methods utilized in this study included sampling, DNA extraction, PCR, electrophoresis, sequencing, and data analysis. The fresh leaves were picked up from Rokan Hulu Regency, Riau Province. In this study, the matK and trnL-trnL-trnF IGS sequences of maman with a length of 754 bp and 938 bp, respectively, had been obtained. The BLASTn analysis based on both sequences showed that maman had 100% similarity to Gynandropsis gynandra. Cleome gynandra was synonymous with G. gynandra. There were 49 nucleotide variations, 16 critical nucleotides, and there were no indels in matK. Meanwhile, in trnL-trnL-trnF IGS there were 181 nucleotide variations, 13 critical nucleotides, and 61 indels. In conclusion, this study succeeded in confirming the taxonomic status of maman from Riau as C. gynandra syn. G. gynandra. The result enriched the abundance of DNA barcode sequences of this plant in GenBank which is useful for the molecular identification.

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