Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Articles
62 Documents
Search results for
, issue
"Vol 9, No 3 (2017): December 2017"
:
62 Documents
clear
<![CDATA[Evaluation of Chromosomal Aberrations and Micronuclei in Medical Workers Chronically Exposed to Low Dose Ionizing Radiation ]]>
<![CDATA[Lusiyanti, Yanti]]>;
<![CDATA[Kurnia, Iin]]>;
<![CDATA[Suvifan, Viria Agesti]]>;
<![CDATA[Sardini, Sardini]]>;
<![CDATA[Purnami, Sofiati]]>;
<![CDATA[Rahajeng, Nastiti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.12382
<![CDATA[Medical workers representing the group is the most consistently are exposed to low doses of ionizing radiation, prolonged low-level ionizing radiation can induce chromosomal aberrations (CAs). This study would evaluate the cytogenetic effect using the CAs based on dicentric, and cytokinesis-blocked micronucleus (CBMN) assay on hospital workers. The exposed group dividedto Interventional and Diagnostic groups then compared to non exposed group. The accumulated absorbed doses calculated for the radiation workers were below 5mSv. Blood samples were obtained from 29 samples of medical workers , and 15 samples of control. The Study showed that the frequency of dicentric chromosomes both in exposed and control were not found. In case of micronuclei, the mean frequencies were observed in exposed group that was (19 6.22) and (16.25 6.04) respectively and the control group was (10.47.79). Frequency MN/1000 cell in the lymphocytes both in the two exposed group was relatively higher compared to control group. However the MN frequencies in all sample group was still in normal range . In this study chronic low radiation dose exposure in the hospital had no significant effect on chromosome aberration nor micronuclei. The benefit of the study is to enrich the potential usefulness of cytogenetic assay providing safety index in medical surveillance programs. The results suggest that education and retraining of staff concerning radiation safety guidelines need to be done to maintain the safety aspects of radiation.]]>
<![CDATA[Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture ]]>
<![CDATA[Yosephine Sri Wulan Manuhara]]>;
<![CDATA[Dannis Yuda Kusuma]]>;
<![CDATA[Rafika Lailiyatul Kurnia Sari]]>;
<![CDATA[Alfinda Novi Kristanti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.9670
<![CDATA[Gynura procumbens has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of G. procumbens in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of G. procumbens adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance. ]]>
<![CDATA[Genetic Variability of Local Corn Cultivars from Kisar Island Southwest Maluku Regency using Microsatellite Molecular Marker ]]>
<![CDATA[Hermalina Sinay]]>;
<![CDATA[Ritha Lusian Karuwal]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.8512
<![CDATA[This research aim was to reveal the genetic variability of local corn cultivars from Kisar Island using microsatellite molecular marker. The use of microsatellite markers is based on one reasons that the genetic information is polymorphic, and will be better to identify genetic diversity of plants including corn. Corn cultivars used were obtained from farmer in Kisar Island, and three reference varieties as outgroup were obtained from Institute of Cereals in Maros South Sulawesi. Polymerase chain reaction was conducted following the protocols from Fast Start kit (Qiagen-USA). The data obtained then analyzed with MVSP 3.1A Software for dendogram construction. The results showed that the appearance of DNA bands was vary (monomorphic and polymorphic) for local cultivars as well as reference varieties, with one or two bands on each primer. Based on the dendogram, there are three main clusters with similarity index ranged from 20% -100%. It can be concluded that there is very low similarity and distant kinship of local cultivars. The novelty of this research is knowing kinship relationship between local corn in Kisar Island which is not known yet. This results is expected to provide benefits regarding the breeding program, and for instance, it can be an important information regarding the development of local corn as a source in assembly the superior corn cultivars. ]]>
<![CDATA[The Effect of Biofertilizer on The Diversity of N2O Reducing Bacteria in Paddy Fields of Sukabumi, Indonesia ]]>
<![CDATA[Alfan Cahyadi]]>;
<![CDATA[Iman Rusmana]]>;
<![CDATA[Nisa Rachmania Mubarik]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.9796
<![CDATA[Some of the methanotrophic bacteria and N2O reducing bacteria have been proven to be able to support the plant growth and increase the productivity of paddy. However effect of the methanotrophic and N2O reducing bacteria application as a biofertilizer to indigenous N2O reducing bacteria is still not well known yet. The aim of this study was to analyze the diversity of N2O reducing bacteria in lowland paddy soil based on a nosZ gene. Soil samples were taken from lowland paddy soils in Pelabuhan Ratu Sukabumi, West Java, Indonesia. There were two treatments for the paddy field soil, ie. biofertilizer-treated field 20% fertilizer (50 kg/ha) with the addition of biofertilizer and 100% fertilizer. PCR amplification of nosZ gene was successfully conducted using nosZF and nosZR primer pair. Denaturing Gradient Gel Electrophoresis (DGGE) process was conducted at 150 V for 5.5h. There were three differences nosZ bands were sequenced. The phylogenetic analysis showed that they were close to uncultured bacteria. Microbial diversity in the biofertilizer-treated field was higher than that of in the 100% fertilizer-treated field. The biofertilizer treatment has higher in microbial diversity than that of applied non-biofertilizer paddy fields. This research might have impact in the application of biofertilizers due to the emission of N2O as a green house gas from paddy fields farming activity. The biofertilizer has great potential application in sustainable environmental friendly agriculture systems.]]>
<![CDATA[Ecological Factors Determining Abundance of Parasitic Mites on Aedes spp. Larvae ]]>
<![CDATA[Nurhadi Eko Firmansyah]]>;
<![CDATA[Susi Soviana]]>;
<![CDATA[Bambang Heru Budianto]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.11458
<![CDATA[Ability to infestation and abundance of parasitic mites in Aedes spp. larvae cannot be separated from the influence of various factors. Ecological factors have been suggested to play a role determine the presence of parasitic mites that under certain conditions become a key factor in determining the abundance of parasitic mites on Aedes spp. larvae. The aim of this study to determine the ecological factors affect the abundance of parasitic mites on Aedes spp. larvae in Bogor Regency. Capturing of Aedes spp. larvae was performed directly on the habitats found in indoor and outdoor. Capturing mites in the body of Aedes spp. larvae was performed using insect forceps. Ecological factors measured were dissolved oxygen (DO), pH, temperature, and total dissolved solid (TDS). The influence of ecological factors was analyzed using regression and correlation analysis. The result of mite identification has been obtained three species of mites that are Halacarus sp., Histiostoma sp., and Hydrozetes sp. The result indicated that total dissolved solid (TDS) and temperature was the factors that determined the abundance of mites. The factors of pH, and dissolved oxygen (DO) did not determine the abundance of parasitic mites of Aedes spp. larvae. The research result can be further developed as a new alternative to Dengue Hemorraghic Fever control and provide information on parasitic mites that infest Aedes spp. larvae. In addition, this results become an early step in controlling of Aedes spp. strategy platform by the parasitic mites.]]>
<![CDATA[The Influence of Environmental Factors on the Diversity of Gastropods in Marsegu Island, Maluku ]]>
<![CDATA[Wiwien Bula]]>;
<![CDATA[Fredy Leiwakabessy]]>;
<![CDATA[Dominggus Rumahlatu]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.10637
<![CDATA[Gastropods are known to have a wide distribution. They can live in a variety of habitats and influenced by various environmental factors. The aim of this study was to investigate the diversity of gastropods and its relationship with the physical and chemical environment of a water. This research was an ecological research with correlational descriptive type. The selection of Marsegu Island was based on the fact that the area is a conservation area, both the forest and sea are frequently visited by tourists. The results of this research showed that there were 22 species of gastropod found in the water of Marsegu Island. The most commonly found was from the genus of Cypraea (5 species). Gastropods found mostly from the family of Strombidae which consisted of 4 genera i.e. Lambis, Canarium, Strombus and Lentigo. The value of gastropod diversity in the three research stations was moderate, 2.93, 2.91 and 2.95 respectively. In addition, there is a correlation between environmental factors and the diversity of gastropods. The simultaneous effect of the independent variables toward the dependent variables can be explained by the magnitude of the determination coefficient (R Square) which was 0528 or 52.8%. Result of this study confirms that the environmental factors greatly affects the diversity of Gastropod in the coastal waters of Marsegu island. The results can provide benefits as control over fishing and coastal pollution due to human activities.]]>
<![CDATA[Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia ]]>
<![CDATA[Afreni Hamidah]]>;
<![CDATA[Evita Anggereini]]>;
<![CDATA[Nurjanah Nurjanah]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.11427
<![CDATA[Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing the number of erythrocytes is 50% papaya leaf juice concentration and 25% papaya leaf juice concentration which gives the best effect in increasing the hemoglobin content. Further, the findings can be used as baseline information for further scientific investigation for using papaya leaf as an alternative medicine in curing anemia disease and analyzing phytochemical, pharmaceutical and other biological activities on other plant or vegetable and to conducted further research by using female mice and pregnant female mice.]]>
<![CDATA[Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent ]]>
<![CDATA[Risa Fitriandini]]>;
<![CDATA[Sri Budiarti]]>;
<![CDATA[Yulin Lestari]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.10323
<![CDATA[Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent. ]]>
<![CDATA[Exploration of Mango Fruits (Mangifera indica) as α-Glucosidase Inhibitors ]]>
<![CDATA[Nadia Prilliane Putri]]>;
<![CDATA[Khalida Shabiba Nursyamsi]]>;
<![CDATA[Yanico Hadi Prayogo]]>;
<![CDATA[Dina Ragillia Sari]]>;
<![CDATA[Eka Budiarti]]>;
<![CDATA[Irmanida Batubara]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.10516
<![CDATA[Mango fruit (Mangifera indica L.) is the tropical fruit that grows easily in Indonesia with plenty varieties. This study aimed to determine the varieties of mango fruit and the most potent part of mango as antidiabetic agent through α-glucosidase inhibitory activities. Four types of mango fruit (indramayu, manalagi, harum manis, and budiraja) were used in this study. Each part of the mango fruit:peel, flesh, endosperm, and endocarp were extracted by maceration process with three different solvents (n-hexane, ethyl acetate (EtOAc), and ethanol (EtOH)). An ability of all 46 extracts in inhibiting the α-glucosidase at a concentration of 500 ppm were determined. Then 11 extracts with the high inhibition value were determined their IC50 (concentration to inhibit 50% activity) values. EtOAc extract of manalagi, indramayu, and budi raja endosperm had the lowest IC50 value which was not statistically significantly different (at 95%) with EtOAc extract of budi raja peel. The bioautographic Thin Layer Chromatogram showed that the most active band is characterized by white luminescence under UV 366 nm, yellow color under UV 254 and visible light. The band with Rf 0.93 from EtOAc endosperm extract of indramayu and manalagi and Rf 0.73 from EtOAc budi raja peel extract are the most active band which predicted as a flavonoid. The result adds the value of the peel and seed of mango, as well as an alternative in blood sugar control, which is easy to obtain, relatively cheap, and liked by the community. ]]>
<![CDATA[Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus ]]>
<![CDATA[Sri Puji Astuti Wahyuningsih]]>;
<![CDATA[Manikya Pramudya]]>;
<![CDATA[Intan Permata Putri]]>;
<![CDATA[Nadyatul Ilma Indah Savira]]>;
<![CDATA[Dwi Winarni]]>;
<![CDATA[Listijani Suhargo]]>;
<![CDATA[Win Darmanto]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v9i3.11284
<![CDATA[Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.]]>
