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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 10 Documents

Search results for , issue "Vol 5, No 1 (2010): (June 2010)" : 10 Documents clear

THE APPLICATION OF RAPD FINGERPRINTING TO ASSESS INBREEDING LEVELS IN THE CULTURED POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii Imron Imron; Dadan Sunandar; Rommy Suprapto; Nunuk Listyowati
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Inbreeding has been one of central issues with regard to genetic quality of aquaculture species, including giant fresh water prawn (GFP). Conventional methods for the estimation of inbreeding level are available, such as pedigree analyses which requires a good pedigree record which, unfortunately, is rarely available. Likewise, microsatellite molecular markers commonly applied to obtain the coefficient inbreeding estimates are both laborious and expensive. Hence, an alternative method of inbreeding assessment which is relatively easy but reliable is in need. This study was aimed to explore the applicability of RAPD fingerprinting, which is known to be simple and affordable, to estimate inbreeding level of GFP population. Three GFP populations namely inbred, outbred, and farm populations with inbreeding level of 25%, 0%, and unknown, respectively, were genotyped using five polymorphic RAPD primers. The inbreeding levels mentioned within the first two populations were determined using pedigree analysis. RAPD banding patterns were then used to calculate band sharing index (BSI) and inbreeding coefficient (F). Assessment of the applicability of inbreeding level estimates obtained by RAPD markers was performed by comparing them to those estimated by pedigree analysis. Results show that RAPD fingerprinting was capable of delineating populations differing in their inbreeding coefficients. The pattern resulted from molecular inbreeding coefficient within the inbred and outbred groups, was congruent with that shown by pedigree analysis, while the farm population showed closeness to the inbred group. While the accuracy of the estimate needs to be verified further, this study suggests that RAPD fingerprinting is applicable to estimate population inbreeding level, particularly due to its technical simplicity and cost affordability.

NONSPECIFIC IMMUNE RESPONSE AND RESISTANCE OF Litopenaeus vannamei FED WITH NUCLEOTIDE, β-GLUCAN, AND PROTAGEN DIETS Henky Manoppo; Sukenda Sukenda; Daniel Djokosetiyanto; Mochamad Fatuchri Sukadi; Enang Harris
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The objective of this research was to evaluate the nonspecific immune response and resistance of Litopenaeus vannamei fed with nucleotide, β–glucan, and protagen diets. Shrimp juveniles with an average weight of 5.39±0.56 g were reared in glass aquaria at a density of 15 shrimps/aquarium. Shrimps were fed three times a day for four weeks at a feeding rate of 3%/bw/day. Treatment diets consisted of A: basal diet (without immunostimulant), B: β–glucan, C: protagen, and D: nucleotide, each with three replicates. At the end of feeding period, the shrimps were intramuscularly injected with Vibrio harveyi 0.1 x 106 cfu.shrimp-1. Total haemocyte count (THC) of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p=0.01), but not different compared to shrimp fed with protagen-diet. PO activity also increased significantly in shrimp fed with nucleotide-diet (p=0.02). β–glucan diet could also increase THC and PO activity, but compared to the control, the increase was not significantly different. Overall, PO activity of shrimp fed with nucleotide, β–glucan, and protagen diets was high (>0.35). Oral administration of nucleotide, β–glucan, and protagen for four consecutive weeks significantly increased resistance of shrimp to disease (<0.01) where the highest resistance rate was observed on shrimp fed with nucleotide-diet. Growth of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p<0.01), as well as to β–glucan, and protagen-treated shrimp. As a conclusion, supplementation of nucleotide into shrimp pellet enhanced nonspecific immune response and growth performance better than β-glucan, and protagen.

INFLUENCE OF FEEDING TIME ON FEED UTILIZATION BY SIAMESE CATFISH, Pangasius hypophthalmus JUVENILE Nina Meilisza; Yann Moreau; Ettiene Baras; Rina Hirnawati
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Siamese catfish (Pangasius hypophthalmus) at juvenile stage is used as ornamental fish by fish hobbies because of their beautiful shape and movement. Feeding time is a part of feeding management to get the best and effective strategy on feed input. This study aimed to determine the growth of Siamese catfish juvenile by looking at the influence of feeding time on feed utilization. The average individual weight of juveniles used was ± 3.2 g stocked 15 fish per aquarium equipped with recirculation system. Two level factors in the factorial design consisted of 4 feeding times (at 8:00, at 12:00, at 16:00, and as control (8:00, 12:00, 16:00) and feeding level (45 g feed kg-1 fish, 90 g feed kg-1 fish). Interaction between feeding time and feeding level resulting eight types of treatments research, namely A (8/45), B (12/45), C (16/45), D (8:12:16/45), E (8:12:16/90), F (8/90), G (12/90), and H (16/90). The results showed that feeding time had significant effect on the specific growth rate, feed conversion, and protein efficiency ratio and were significantly different among the treatments (P<0.05). Besides feeding time control (treatments D and E), treatment C also showed the highest of specific growth rate (2.03%), protein efficiency ratio (1.46), and the lowest feed conversion ratio (1.85).

OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus) Raden Roro Sri Pudji Sinarni Dewi; Alimuddin Alimuddin; Agus Oman Sudrajat; Komar Sumantadinata; Sularto Sularto
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.

IMPROVEMENT OF NON SPECIFIC IMMUNE SYSTEM ON COMMON CARP (Cyprinus carpio) AGAINST KOI HERPESVIRUS DISEASE (KHVD) BY ADDITION OF ASCORBIC ACID ON FISH DIET: A FIELD SCALE STUDY Taukhid Taukhid; Desy Sugiani; Angela Mariana Lusiastuti
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The research with the aim to know the optimal feeding frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) on the dose of 750 mg/kg feed to control Koi Herpesvirus (KHV) disease infecting common carp has been done in field condition. Fish were reared in floating cages with the size of 3.5 m x 3.5 m x 2.0 m and stocking density of 1,250 fish/cage with the size range of ± 10 g/fish. The treatments applied in the research were: (A) daily application, (B) every 3 days application, and (C) without vitamin C addition as the control. Fish test were challenged to KHV infection on the mid cultivation by cohabitation method in the laboratory scale for 2 weeks. Examination on behavior, clinical sign, and mortality of fish test conducted daily. The results showed that the highest survival rate was found on the application of vitamin C every 3 days (60.16%); and followed by every day (52.00%), and the lowest was found on the control group (47.36%).

USE OF SPONGE, Callyspongia basilana EXTRACT AS ADDITIVE MATERIAL ON TIGER SHRIMP CULTURE Rosmiati Rosmiati; Emma Suryati; Arifuddin Tompo
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Blue shrimp disease is one of the main problems in tiger shrimp culture. It reduces shrimp quality which eventually will decrease its market price. Blue shrimp is caused by deficiency of nutrition and additive materials such as carotene and other nutrient which function as vitamin source for important metabolic processes and formation of color profile in shrimp and fish. The aims of this study were to study the application effect of carotenoid extract of sponge Callyspongia basilana, as an additive material on the ability of shrimp to get back to normal state after suffering blue shrimp disease and survival rate of shrimp and to find out the optimal concentration of sponge carotenoid extract to cure the diseased shrimp. This study was consisted of two steps namely; (1). Extraction of sponge carotenoid by maseration and fractionation using acetone and petroleum ether solvents and (2), the application of carotenoid extract on the diseased shrimp. The research was arranged in a complete randomized design with four experiments consisted of (A). Control (without carotenoid extract); (B),(C), and (D) carotetoid extract addition of 3 mg/L, 6 mg/L, and 9 mg/L respectively with three replication each. The test animal used were blue diseased tiger shrimp with the density of 15 ind./container having 7.5–9.5 cm in size and the average weight of 5.5–10.0 g. The study showed that Callyspongia basilana carotenoid extract was able to change blue diseased shrimp to be normal within six days at the concentration of 9 mg/L. The highest survival rate was found in the experiment D (93.3%). Meanwhile, the lowest was obtained by the control population (13.3%) and the other two treatments were 80.0%(C) and 73.3% (B). The average of water quality parameters such as temperature, dissolved oxygen, pH, salinity, nitrite, and ammonia were in the suitable range for the growth and survival rate of tiger shrimp.

ULTRASTRUCTURAL ANALYSIS OF GRUNT FIN (GF) CELLS TREATED WITH RED SEA BREAM IRIDOVIRUS (RSIV; family Iridoviridae, genus Megalocytivirus) IN COMBINATIONS WITH INTERFERONS AND SPLENIC SUBSTANCES Ketut Mahardika; Teruo Miyazaki
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The genus Megalocytivirus in the family of Iridoviridae encompasses isolate of red sea bream iridovirus (RSIV). In the present study, grunt fin (GF) cells were treated with red sea bream iridovirus (RSIV) in combinations with interferons (IFNs) and splenic substances. The viral titer in the combination with primary splenic substance was higher than the other combinations of 10-1 and 10-2 diluted splenic substances, and the positive control. The viral titer was not decreased by all combinations with recombinant murine interferon-α (rMuIFN-α), recombinant murine IFN-β (rMuIFN-β), and recombinant feline interferon-ω (rFeIFN-ω). Electron microscopy revealed inclusion body bearing cells (IBCs) and enlarged cells allowing virus propagation within the intracytoplasmic virus assembly site (VAS). Most were enlarged cells. These enlarged cells were divided into three cell types. Cells of Type II, which contained many mature virions within the VAS, were numerous in number in all treated cells. Cells of Type I allowing assembly of few virions and cells of Type III containing many immature viral particles were rather fewer in number. Their percentage was almost the same in all combinations with the splenic substances and IFNs. These results determined in in vitro treatment with IFNs did not prevent viral replication of RSIV, as well as the splenic substances which were derived from the RSIV-infected spleen of red sea bream did not contain any factors to disturb RSIV replication.

HIGH DENSITY JUVENILE SHRIMP (Penaeus monodon) PRODUCTION WITH DIFFERENT DURATIONS OF REARING IN ACID SULFATE SOIL-AFFECTED POND Akhmad Mustafa; Jesmond Sammut
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

An experiment was conducted to evaluate the effectiveness of producing juvenile shrimp in a reservoir pond constructed in acid sulphate soils (ASS). Many ponds constructed in ASS pose a significant economic risk due to high mortality and poor growth. In Indonesia, abandonment of ponds in ASS is widespread and alternative farming practices, such as production of juvenile shrimp, offer farms opportunities to lower their production risks. The experiment involved rearing juvenile shrimp at a high stocking density (3,000 individuals/m2) using hapas in a formerly unproductive pond located in Luwu Regency, South Sulawesi, Indonesia. The treatments tested included rearing periods of three and six weeks with four replications. A survival of 83.62% was achieved after rearing for three weeks compared to 45.44% after six weeks. Although there was a sixfold increase in the mean weight of shrimp from three to six weeks of culture, size variation after six weeks was large due to the high density of shrimp. The experiment showed that old acid sulphate soil-affected ponds, in which the bottom soil has not been excavated any further, can be used for production of juvenile shrimp in hapas. Rearing over three weeks is recommended to obtain high survival and reduce size variation.

THE EFFECT OF VITAMIN C (L-ASCORBYL MONOPHOSPHATE-Mg) ON THE DEFORMITY PERFORMANCE OF HUMPBACK GROUPER (Cromileptes altivelis) LARVAE Sophia Lasma Sagala; Suko Ismi; I Nyoman Adiasmara Giri
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This study was aimed to get a standard protocol for vitamin C application in enrichment of Artemia nauplii and to examine the impact of vitamin C on humpback grouper larval deformity performance. Vitamin C in the form of L-ascorbyl-2-monophosphate-Mg (AMP-Mg) was used for the enrichment and incorporated into Artemia nauplii at a given level of 0-1.5 g/L with enrichment period of 4, 6, and 24 hours for each level. The effect of AMP on larval deformity was tested using the following treatments: unenriched both Artemia nauplii and commercial diet, un-enriched Artemia nauplii and experimental diet containing 2.5 g AMP-Mg/kg diet, enriched Artemia nauplii obtained from the protocol and commercial larval diet. Analysis of vitamin C content in Artemia nauplii was carried out using HPLC and larval deformities were observed using staining method, visual examination, or X-ray depending on larval age. The results showed that the best enrichment method of AMP in Artemia nauplii is achieved at the dose of 0.9 g/L for 6 h. The lowest percentage of abnormalities was found in larvae fed with enriched Artemia nauplii and commercial diets, suggesting that vitamin C has an important role in controlling larval deformities.

PRODUCTION AND BIOACTIVITY POTENTIAL OF THREE RECOMBINANT GROWTH HORMONES OF FARMED FISH Alimuddin Alimuddin; Indra Lesmana; Agus Oman Sudrajat; Odang Carman; Irvan Faizal
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1oC for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species.

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Home Page

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Contact Name
Ofan Bosman

Contact Email
Ofan Bosman

Phone
-

Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA