Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Riset Akuakultur

jra
Website

Published by Politeknik Kelautan dan Perikanan Jembrana

ISSN : 19076754 EISSN : 25026534 DOI : http://doi.org/10.15578/JRA

Core Subject : Agriculture, Social,

Agriculture, Biological Sciences & Forestry Environmental Science

Jurnal Riset Akuakultur as source of information in the form of the results of research and scientific review (review) in the field of various aquaculture disciplines include genetics and reproduction, biotechnology, nutrition and feed, fish health and the environment, and land resources in aquaculture

Arjuna Subject : Umum - Umum

Articles 10 Documents

Search results for , issue "Vol 12, No 2 (2017): (Juni 2017)" : 10 Documents clear

KARAKTERISTIK FENOTIPE DAN GENOTIPE IKAN GURAMI, Osphronemus goramy, STRAIN GALUNGGUNG HITAM, GALUNGGUNG PUTIH, DAN HIBRIDANYA Otong Zenal Arifin; Imron Imron; Nandang Muslim; Ade Hendri; Aseppendi Aseppendi; Akhmad Yani
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Persilangan antara dua populasi yang berbeda secara genetik lazimnya menghasilkan kombinasi genetik baru. Penelitian ini dilakukan untuk mengevaluasi karakteristik fenotipe dan genotipe ikan gurami strain Galunggung Hitam, Galunggung Putih, dan Hibridanya. Perkawinan dalam galur (GP X GP dan GH X GH) dan persilangan dua arah antara betina GH dan jantan GP (GH X GP), dan resiprokalnya (GP X GH) telah dilakukan di BPBIGN Singaparna. Fenotipe dianalisis berdasarkan metode truss morfometrik dan meristik, sedangkan karakteristik genotipe diamati menggunakan metode PCR-RAPD. Hasil analisis menunjukkan berdasarkan 21 karakter truss morfometrik, centroid hibrida GH X GP lebih tinggi dari centroid ketiga populasi lainnya pada PC-1 yang dicirikan oleh karakter B3, B4, B5, A3, A4, dan A6, dan bersifat intermediate pada PC-2, PC-3, dan PC-4. Berdasarkan delapan karakter meristik, centroid hibrida GH X GP lebih tinggi dari kedua tetua dan hibrida GP X GH pada PC-1 yang dicirikan oleh karakter SPR dan SDA, lebih rendah dari kedua tetua, tetapi lebih tinggi dari populasi GP X GH pada PC-2, dan lebih rendah dari ketiga populasi lainnya pada PC-3 dan PC-4. Polimorfisme dan heterozigositas pada populasi hibrida GH X GP dan GP X GH lebih tinggi daripada kedua tetuanya GH X GH dan GP X GP. Hibridisasi yang dilakukan meningkatkan variasi genetik yang dapat berguna dalam peningkatan produktivitas budidaya.Hybridization between two genetically different populations is expected to generate a population carrying new genetic combinations which may be expressed in both phenotypes and genotypes. This study was carried out to explore the phenotype and genotype characteristics of reciprocal hybrids of goramy of Galunggung black (GH) and Galunggung white (GP) with respect to their parental lines. Matings within the same line producing GP X GP and GH X GH, and reciprocal mating producing hybrids GH X GP and GP X GH, were conducted at BPBIGN Singaparna. Samples representing the four populations were analyzed for their morphology using truss morphometric and meristic methods, while genotypes were analyzed using PCR-RAPD method. The results showed that based on the 21 morphometric characters, the centroid of hybrid GH X GP was higher than those of the other three populations at PC-1, which was marked by the characters B3, B4, B5,A3, A4, and A6, and was intermediate at PC-2, PC-3, and PC-4. Based on the eight meristic characters, the centroid of GH X GP was also higher than those of their parents and hybrids GP X GH at PC-1, which was marked by the characters SPR and SDA, lower than those of their parents but higher than that of hybrid GP X GH at PC-2, and lower than those of the other three populations at PC-3 and PC-4. The genetic diversities in terms of polymorphism and heterozygosity levels in hybrids GH X GP and GP X GH were higher than those found in the pure-line (GH X GH and GP X GP). Hybridization conducted in this study had resulted in the enhancement of genetic variations which could be useful in increasing aquaculture productivity.

KERAGAAN REMATURASI GONAD INDUK TERIPANG PASIR, Holothuria scabra DENGAN PEMBERIAN JENIS PAKAN BERBEDA Sari Budi Moria Sembiring; Ida Komang Wardana; Nyoman Adiasmara Giri; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Rematurasi gonad induk teripang pasir Holothuria scabra yang sudah memijah masih banyak masalah, padahal rematurasi diperlukan untuk keberlanjutan pemijahan dan penyediaan benih. Tujuan penelitian adalah memperoleh jenis pakan yang dapat mempercepat proses rematurasi gonad terhadap induk alam yang sudah dipijahkan sehingga dapat memenuhi kebutuhan untuk pembenihan teripang di hatcheri, serta mengetahui performa benih yang dihasilkan secara fenotipe dan genotipe. Penelitian rematurasi gonad dengan pemberian pakan berbeda telah dilakukan di Balai Besar Penelitian dan Pengembangan Budidaya Laut, Gondol. Penelitian dilakukan dengan metode eksperimental secara rancangan acak lengkap dengan perlakuan perbedaan jenis pakan, yaitu: A) Ulva sp. dan bentos; B) Gracilaria sp. dan bentos, dan C) bentos saja. Dosis pemberian pakan sebanyak 4% dari bobot badan dengan frekuensi pemberian 1 kali/hari. Setiap perlakuan terdiri atas tiga ulangan. Jumlah induk setiap ulangan sebanyak tiga ekor dengan ukuran panjang dan bobot 13,08 ± 2,04 cm dan 182,75 ± 47,74 g. Benih yang dihasilkan sebelum induk dirematurasi dan setelah rematurasi diamati performa fenotipe dan genotipenya. Analisis genotipe menggunakan metode mikrosatellit dengan empat lokus (Hsc-11; Hsc-28; Hsc-49; dan Hsc-59). Parameter yang diamati meliputi frekuensi pemijahan induk teripang, tingkat kematangan gonad, indeks gonadosomatik, fekunditas, diameter telur, pertumbuhan benih F-1 sebelum dan sesudah rematurasi, serta performa genotipe benih tersebut. Data indeks gonad somatik dan diameter telur dianalisis dengan ANOVA, sedangkan data pertumbuhan, pemijahan, dan fekunditas disajikan dalam bentuk tabel dan grafik. Hasil penelitian menunjukkan bahwa semua perlakuan dapat mempercepat rematurasi gonad dan induk teripang memijah setelah dua bulan pemeliharaan. Jenis pakan bentos menghasilkan indeks gonadosomatik dan diameter telur lebih baik dibandingkan dengan perlakuan pakan lainnya (P<0,05). Keragaman genetik benih dari induk sebelum rematurasi (0,719) berbeda nyata (P<0,05) dengan sesudah rematurasi (0,634); sedangkan secara fenotipe, pertumbuhan panjang, dan bobot benih hingga umur 150 hari tidak berbeda (P>0,05).Successful rate of gonad re-maturation of sea cucumber, H. scabra broodstock after spawning still faces some problems presumably due to lack of essential nutrients to support the process. However, re-maturation is vitally important to speed up the fingerling production. The aim of the research was to determine the best feed composition able to induce gonad re-maturation of sea cucumber natural broodstock which had been previously spawned. In addition, this research was also aimed to study the fingerling genetic performance, both phenotypically and genetically. The research on gonad re-maturation was carried out using different feeds at the Institute for Mariculture Research and Development, Gondol. The research was designed in a completely randomized design with three different feed compositions as treatments: A) Ulva sp. and benthos; B) Gracilaria sp. and benthos, and C) only benthos. The amount of given feed was 4% of the total biomass and once daily. Each treatment has three replicates. The number of broodstock in each replicate was three individuals with the averages of total length and body weight were 13.08 ± 2.04 cm and 182.75 ± 47.74 g, respectively. Fingerlings produced from natural maturation and re-maturation broodstock were phenotypically and genetically analyzed. Microsatellite method was used for the genetic analysis of four loci (Hsc-11; Hsc-28; Hsc-49; and Hsc-59). The parameters measured were gonad maturity level, gonadosomatic index, spawning frequency, fecundity, eggs diameter, phenotypic, and genetic performance of F-1 fingerlings produced from both natural maturation and re-maturation broodstock and compared to each other. Data on gonadosomatic index and eggs diameter were statistically analyzed using Anova-test, while growth rate, spawning, and fecundity were descriptively compared using tables and figures. The results showed that all feed treatments were able to induce gonad re-maturation and all sea cucumber broodstock re-spawned after two months. Benthos feed had a significant effect in increasing gonad somatic index and eggs diameter compared to the other feed treatments (P<0.05). Genetic variance of F-1fingerlings produced from the natural maturation broodstock (0.719) was significantly different (P<0.05) compared to the fingerlings produced from the re-maturated broodstock (0.634). However, phenotypically, the growth of length and weight after 150 days old fingerling was not significantly different (P>0.05) between the two fingerling generations.

KARAKTERISASI DAN EVALUASI POPULASI ABALON Haliotis squamata SECARA MOLEKULER, MORFOMETRIK, DAN BIOLOGI Gusti Ngurah Permana; Rudhy Gustiano; Ibnu Rusdi; Fitriyah Husnul Khotimah; Bambang Susanto; Dedi Duryadi Solihin
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Abalon merupakan salah satu komoditas penting gastropoda laut. Tingginya permintaan abalon ini mengakibatkan menipisnya stok di alam. Oleh karena itu, upaya keberhasilan budidaya abalon perlu didukung oleh jenis unggul. Indikasi awal suatu jenis unggul dapat dilakukan dengan menganalisis potensi genetik yang dimiliki. Penelitian ini dilakukan dengan analisis gen 16S rRNA, karakter morfolologi, dan biologi dianalisis secara deskriptif dengan metode kajian pustaka. Hasil yang diperoleh menunjukkan keragaman inter populasi Haliotis squamata mendeteksi adanya tujuh haplotipe yang terbagi dalam dua kelompok. Penyertaan H. diversicolor sebagai outgroup dalam pengujian memperlihatkan bahwa populasi H. squamata dari Pulau Bali dan beberapa lokasi di Pulau Jawa berada dalam satu kelompok yang terpisah dengan outgroup. Hasil ini kongruen dengan analisis morfometrik terdapat perkembangan pertumbuhan cangkang yang asimetri pada populasi Banten. Pertumbuhan asimetri merupakan indikasi spesifik untuk populasi Banten atau merupakan gejala abnormalitas yang dapat diakibatkan oleh faktor penurunan kualitas genetik atau lingkungan. Karakter biologi terlihat proporsi daging dan gonad berbeda pada populasi Banten dengan indikasi adanya pertumbuhan asimetri. Rasio gonad dan daging populasi Banyuwangi berbeda nyata (P<0,05) dengan populasi lainnya.Abalone is arguably one of the highly valued and sought-after marine gastropods. However, the over-exploitation of this species has exhausted its wild stock. To overcome this challenge, the culture technique and management of this species must be established and continually improved. One of the ways is through producing superior broodstocks. An initial assessment of a genetically superior broodstock can be done using the potential genetic analysis. This recent research employed the analysis to study the species’ 16S rRNA gene. To complement the study, the morphometric and biological characteristics of the species were analyzed descriptively with the aid of scientific literature. The results showed that the interpopulation diversity of Haliotis squamata was detected by the presence of seven haplotypes divided into two groups. The inclusion of H. diversicolor as an outgroup within the test showed that the populations of abalone in Bali and several other sites in Java were genetically separated from the outgroup. This finding can be backed up with the result of the morphometric analysis where there was asymmetric shell growth in Banten abalone population. This asymmetric growth is considered as a symptom of abnormality caused by genetic or environmental degradation factors. The biological characteristics showed the different ratios of meat and gonad in the Banten population due to the asymmetric growth. Banyuwangi population was significantly different (P<0.05) from other populations in terms of meat and gonad ratios.

LAJU RESPIRASI INDUK IKAN BLACKHEAD SEABREAM Acanthopagrus schlegelii PADA SUHU PEMELIHARAAN YANG BERBEDA Vitas Atmadi Prakoso; Young Jin Chang
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Laju respirasi hewan akuatik memiliki hubungan yang erat dengan metabolisme. Tingkat metabolisme hewan merupakan variabel yang dapat dipengaruhi faktor dalam maupun luar, salah satunya adalah suhu. Pada ikan, proses metabolisme juga berkorelasi dengan suhu. Salah satu jenis ikan yang perlu dikaji laju respirasinya adalah ikan blackhead seabream Acanthopagrus schlegelii yang merupakan spesies ikan laut yang popular di Korea Selatan, sehingga diperlukan lebih banyak informasi lagi mengenai laju respirasi ikan ini untuk mengoptimalkan metabolisme ikan ini dan berdampak kepada produktivitas budidaya. Penelitian ini bertujuan untuk mengevaluasi laju respirasi induk ikan blackhead seabream pada beberapa tingkatan suhu. Ikan blackhead seabream (panjang total 29,3 ± 2,2 cm dan bobot tubuh 538,3 ± 43,0 g) diteliti menggunakan respirometer dalam sistem resirkulasi. Tiga kelompok percobaan dilakukan untuk mengukur laju respirasi berdasarkan perubahan suhu pemeliharaan (15°C, 20°C, dan 25°C). Hasil penelitian menunjukkan bahwa kecenderungan laju respirasi meningkat secara linier dengan peningkatan suhu perlakuan, dengan nilai tertinggi sebesar 164,8 ± 30,7 mg O2/kg/jam pada 25°C dan nilai terendah sebesar 72,4 ± 8,1 mg O2/kg/jam pada 15°C. Sementara itu, frekuensi pernapasan berkorelasi positif terhadap kenaikan suhu. Berdasarkan penelitian ini, dapat disimpulkan bahwa perubahan suhu dari 15°C ke 25°C menyebabkan peningkatan laju respirasi pada induk ikan blackhead seabream.It is well known that a close relationship exists between respiration rate and metabolism in aquatic animal. In fish, the metabolic rate is influenced by internal or external factors, such as temperature. This research observed the respiration rate of blackhead seabream, Acanthopagrus schlegelii, which is one of the popular marine fish species in South Korea. Despite the fish popularity, very few information are available about the fish’s respiration rate which is important in order to optimize its metabolism and increase its aquaculture productivity. This study aimed to evaluate the respiration rate of adult blackhead seabream reared in media with different temperature settings. The fish (total length of 29.3 ±2.2 cm and body weight of 538.3 ± 43.0 g) were observed using a respirometer placed inside the recirculation systems. Three groups of experiments were set up to measure the fish’s respiration rate according to different rearing temperatures (15°C, 20°C, and 25°C). The results showed that there was a tendency of respiration rate increased linearly with the increase of temperature. The highest respiration rate was 164.8 ± 30.7 mg O2/kg/hour at 25°C and the lowest value was 72.4 ± 8.1 mg O2/kg/hour at 15°C which indicated that the respiratory rate positively correlated to the change in temperature. According to this study, it can be concluded that temperature changes from 15°C to 25°C led an increase in respiration rate of adult blackhead seabream

PERFORMA PEMBESARAN IKAN MAS RAJADANU (Cyprinus carpio) GENERASI KETIGA HASIL SELEKSI “WALKBACK” yogi Himawan; Khairul Syahputra; Didik Ariyanto
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Timbulnya wabah penyakit KHV (Koi herpesvirus) pada budidaya ikan mas telah menurunkan produksi ikan mas nasional secara signifikan. Beberapa langkah dilakukan untuk mengatasi penyakit tersebut, salah satunya pembentukan ikan mas Rajadanu tahan KHV melalui seleksi. Dalam rangka memperoleh varietas unggul ikan mas tahan KHV pada tahun 2014 telah dilakukan pembentukan populasi F-3 melalui program selective breeding dengan metode walkback selection. Tujuan penelitian ini adalah menganalisis performa pertumbuhan ikan mas Rajadanu generasi ketiga hasil seleksi melalui uji skala lapang pada fase pembesaran, serta kegiatan uji tantang pada skala laboratorium. Benih F-3 merupakan hasil pemijahan F-1 betina dan F-2 jantan hasil seleksi positif MHC (Major Histocompatibility Complex), sedangkan kontrol berupa benih ikan mas Majalaya dari pembudidaya, masing-masing tiga ulangan. Uji tantang KHV dilakukan di akuarium berukuran 60 cm x 40 cm x 40 cm di hatchery ikan mas dengan suhu permissive 21°C melalui metode kohabitasi. Hasil penelitian menunjukkan bahwa populasi F-3 ikan mas Rajadanu hasil seleksi memiliki performa pertumbuhan relatif lebih baik 6,20% dibandingkan dengan kontrol pada karakter panjang total (18,14±4,90 cm), 5,80% lebih baik pada karakter panjang standar (14,76 ± 4,39 cm); 38,39% lebih baik pada karakter bobot rata-rata (393,98 ± 3,53 g); 16,83% pada karakter laju pertumbuhan spesifik (2,29 ± 0,04%); dan 3,80% pada karakter sintasan (49,13 ± 6,88%). Hasil uji tantang KHV menunjukkan sintasan populasi F-3 lebih tinggi (96,6 ± 3,3%) dibandingkan dengan kontrol (51,1 ± 8,3%).The outbreaks of KHV (Koi herpesvirus) disease in carp culture have significantly reduced Indonesia’s national carp production. Several strategies have been devised to combat the outbreaks including the development of KHV resistant carp varieties such as selective carp breeding program in 2014. The purpose of this study was to analyze the growth performance of the third generation (F-3) Rajadanu through field-scale test (grow out) and challenge test (laboratory-scale). The study was designed in a completely randomized design with two treatments (F-3 populations) and carp seed from the local small-scale fish farmers as control, each with three replicates. F-3 seed was produced from spawning between F-1 females and F-2 males which had a Major Histocompatibility Complex positive selection. The challenge test was carried out in the aquarium sized 60 cm x 40 cm x 40 cm at the hatchery with the permissive temperature of 21°C. The results showed that F-3 Rajadanu population had relatively higher total length (18.14 ± 4.90 cm) of 6.20%, standard length (14.76 ± 4.39 cm) of 5.80%, weight average (393.98 ± 3.53 g) of 38.39%, specific growth rate (2.29 ± 0.04%) of 16.83%, and survival rate (49.13 ± 6.88%) of 3.80% than that of control population. The result of the challenge test on KHV in the laboratory showed that F-3 population had higher survival rate (96.6 ± 3.3%) compared to that of control (51.1 ± 8.3%).

INDUKSI OVULASI DAN PEMIJAHAN IKAN AGAMYSIS (Agamyxis albomaculatus) MENGGUNAKAN HORMON YANG BERBEDA Bastiar Nur; Asep Permana; Agus Priyadi; Siti Zuhriyah Mustofa; Siti Murniasih
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ikan agamysis (Agamyxis albomaculatus) merupakan ikan hias air tawar introduksi yang memiliki potensi cukup bagus untuk dikembangkan di Indonesia, namun masih bermasalah dalam budidayanya. Penelitian ini bertujuan untuk menguji efektivitas kombinasi hormon berbeda dalam menginduksi ovulasi dan pemijahan ikan agamysis. Jumlah ikan yang digunakan sebanyak 30 ekor (15 jantan dan 15 betina) dengan ukuran panjang 10-15 cm dan bobot 50-200 g. Ikan uji dipelihara dalam akuarium sistem resirkulasi dan diberi pakan cacing tanah dua kali sehari secara ad libitum. Perlakuan yang diberikan berupa penggunaan beberapa jenis hormon yaitu: (A) LHRHa dan antidopamin (ovaprim) dosis 0,7 mL/kg; (B) human chorionic gonadotropin (hCG) dosis 500 IU/kg; (C) aromatase inhibitor (AI) dosis 10 mg/kg; (D) kombinasi AI dosis 10 mg/kg + ovaprim dosis 0,7 mL/kg; serta (E) kombinasi hCG dosis 500 IU/kg + ovaprim dosis 0,7 mg/kg. Hormon diberikan melalui penyuntikan secara intramuskuler. Hasil penelitian menunjukkan bahwa penggunaan kombinasi hormon hCG dosis 500 IU/kg + ovaprim dosis 0,7 mL/kg; memberikan respons pemijahan terbaik dengan waktu laten 7-12 jam; bobot telur ovulasi 7,3–9,4 g; derajat pembuahan 5,71%-34,7%; dan derajat penetasan 32,5%-50,44%. Penggunaan kombinasi hormon hCG dan ovaprim efektif menstimulasi proses ovulasi telur sehingga dapat menjadi acuan untuk peningkatan produksi larva ikan agamysis dalam pemijahan buatan.Agamysis (Agamyxis albomaculatus) is an introduced freshwater fish that has the potential to be developed in Indonesia. Yet, the fish has not been successfully cultivated. This research was conducted to determine the effectiveness of different hormone combinations to induce ovulation and spawning of agamysis. This research used 30 fishes (15 male and 15 female) ranged 10-15 cm in length and 50-200 g in body weight. The fish were stocked in aquaria equipped with recirculation system and fed with earthworm by ad libitum twice a day. The treatments were: (A) LHRHa and antidopamin (ovaprim) dose of 0.7 mL/ kg, (B) human chorionic gonadotropin (hCG) dose of 500 IU/kg, (C) aromatase inhibitor (AI) dose of 10 mg/kg, (D) a combination of AI dose of 10 mg/kg + ovaprim dose of 0.7 mL/kg, and (E) a combination of hCG dose of 500 IU/kg + ovaprim dose of 0.7 mg/kg. The hormones were given through intramuscularly injection. The results showed that the combination of ovaprim dose of 0.7 mL/kg and hCG dose of 500 IU/kg produced the best spawning response, with the latent period of 7-12 hours, weight of ovulated eggs of 7.3-9.4 g, fertilization rate of 5.71% to 34.7% and hatching rate between 32.5% - 50.44%. In conclusion, the combination of hCG and ovaprim was found to be more effective to stimulate egg ovulation of agamysis and could be used in improving larvae production.

PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI Ahmad Muzaki; Sari Budi Moria Sembiring; Ida Komang Wardana; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Pertumbuhan dan sintasan larva dan benih kerapu sunu turunan ketiga (F-3) dari induk turunan kedua (F-2) hasil seleksi telah diamati di Balai Penelitian dan Pengembangan Budidaya Laut, Gondol. Induk F-2 diseleksi menggunakan marka indikator tumbuh cepat PL-03 (alel tunggal berukuran 370 bp). Penelitian ini bertujuan untuk mengevaluasi keragaan pertumbuhan dan sintasan larva dan benih kerapu sunu F-3 serta pewarisan sifat tumbuh cepat dari induk kerapu sunu (F-2) kepada benih kerapu sunu F-3. Pembenihan menggunakan induk kerapu sunu F-2 dengan sifat tumbuh cepat (membawa marka PL-03) dan tanpa sifat tumbuh cepat. Telur dan larva yang dihasilkan dipelihara sebagai turunan F-3 hingga berukuran benih dan dibesarkan selama 10 bulan pemeliharaan. Hasil pengamatan sintasan larva kerapu sunu F-3 dari induk membawa marka PL-03 adalah sebesar 10,6% ± 9,1%; sedangkan larva F-3 dari induk yang tidak membawa sifat tumbuh cepat hanya sebesar 2,7% ± 1,7%. Benih F-3 dari induk membawa marka PL-03 dapat dipanen saat mencapai ukuran 2,5-3,0 cm pada umur 38 ± 2 hari, sedangkan benih F-3 dari induk tanpa marka PL-03 pada umur 40 ± 1 hari. Selanjutnya benih kerapu sunu F-3 dari induk yang mempunyai marka PL-03 yang dipelihara selama 10 bulan memiliki laju pertumbuhan harian lebih baik (3,11%) dibandingkan benih kerapu sunu F-3 tanpa marka PL-03 (3,01%). Sintasan benih kerapu sunu F-3 dengan marka PL-03 tidak berbeda nyata dengan yang tidak mempunyai marka PL-03. Persentase benih F-3 turunan dari induk F-2 dengan sifat tumbuh cepat yang membawa marka PL-03 sebesar 45%.The growth and survival rate of the third generation (F-3) of coral trout grouper larvae and fry from the genetically selected broodstock were observed at the Institute for Mariculture Research and Development, Gondol. The F-2 broodstock were selected using the fast-growing marker indicator, PL-03 (single allele of 370 bp in size). The aim of this study was to determine the growth performance and survival rate of larvae and fry of F-3 coral trout groupers, as well as inheritance of fast growth trait from the F-2 broodstock to the F-3. The eggs and larvae of F-3 produced from F-2 broodstock with fast growth trait (having PL-03 marker) and without fast growth trait were reared to reach fry stage for 10 months. The results showed that the survival rate of the F-3 larvae from broodstock with PL-03 marker was 10.6% ± 9.1%, while F-3 larvae from the broodstock without fast growth trait only reached 2.7% ± 1.7%. Fry (F-3) with PL-03 marker reached the optimum harvest size of 2.5-3.0 cm at 38 ± 2 days old, while the F-3 fry of broodstock without PL-03 marker took relatively longer to reach that size (40 ± 1 days age). The F-3 from the broodstock with PL-03 marker reared for 10 months had better growth rate (3.11%) compared to F-3 fry without PL-03 marker (3.01%). The survival rate of F-3 fry with PL-03 marker was not significantly different to F-3 fry without PL-03 marker. The percentage of F-3 fry from the broodstock of F-2 with fast growth trait was 45%.

EFEKTIVITAS KOMBINASI PROBIOTIK MIKROENKAPSULASI MELALUI PAKAN UNTUK PENGENDALIAN PENYAKIT MOTILE AEROMONADS SEPTICEMIA PADA IKAN LELE, Clarias gariepinus Angela Mariana Lusiastuti; Septyan Andriyanto; Reza Samsudin
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini bertujuan untuk mengetahui efektivitas kombinasi probiotik mikroenkapsulasi melalui pakan dalam pengendalian penyakit pada ikan lele, Clarias gariepinus. Penelitian dilakukan mulai dari penyiapan kombinasi probiotik Bacillus cereus ND2 dan Staphylococcus lentus L1K, mikroenkapsulasi probiotik, uji probiotik mikroenkapsulasi, serta uji dosis secara in vivo. Proses mikroenkapsulasi menggunakan BUCHI mini spray dryer dengan suhu inlet 131°C-133°C dan suhu outlet 65°C-70°C. Pakan yang digunakan adalah pakan buatan dengan kadar protein 38% yang dicampur dengan probiotik mikroenkapsulasi sesuai dengan dosis perlakuan dan diberi putih telur 2% sebagai binder. Perlakuan yang dilakukan adalah membandingkan secara in vivo probiotik mikroenkapsulasi (PM) dan probiotik non-mikroenkapsulasi (PNM), dan dilanjutkan pengujian dosis melalui pakan untuk memperoleh dosis terbaik dari aplikasi probiotik mikroenkapsulasi. Parameter pengamatan berupa tingkat sintasan, biomassa, kadar hematokrit, hemoglobin, respiratory burst activity, dan total bacterial count. Hasil penelitian menunjukkan bahwa suplementasi kombinasi probiotik mikroenkapsulasi Bacillus cereus ND2 dan Staphylococcus lentus L1K pada pakan lebih efektif, dan dengan dosis 2% menunjukkan hasil yang lebih baik dalam meningkatkan sintasan hingga 97,33%, biomassa dan respons imun (NBT assay, hematocrit, hemoglobin) ikan lele terhadap A. hydrophila.This study was aimed to know the effectivity of microencapsulated probiotic combinations administered through diet to control motile aeromonads septicemia disease in catfish (Clarias gariepinus). The research stages consisted of preparation probiotics, microencapsulation of probiotics and probiotic microencapsulation testing in vivo. BUCHI mini spray dryer was used in the microencapsulation process with inlet temperature ranged between 131°C-133°C and outlet temperature ranged between 65°C-70°C. The diets used in the research were pelleted feeds with a protein content of 38% and mixed with different dosages of microencapsulated probiotics. Egg albumen (2%) was used to bind the mixture. The effectivity of microencapsulated and non-microencapsulated feeds was compared in order to find an optimum microencapsulation dosage. The observed parameters were survival rate, biomass, hematocrit, hemoglobin, respiratory burst activity, and total bacterial count. The results showed that the supplementation of microencapsulated probiotic Bacillus cereus ND2 and Staphylococcus lentus L1K in the diets was more effective at a dose of 2%, showing better survival rate (97.33%), biomass gain, and immune responses (NBT assay, hematocrit, hemoglobin) against A. hydrophila in catfish Clarias gariepinus.

KARAKTERISTIK DAN KESESUAIAN PERAIRAN UNTUK BUDIDAYA RUMPUT LAUT DI KABUPATEN KEPULAUAN SANGIHE, SULAWESI UTARA Andi Akhmad Mustafa; Tarunamulia Tarunamulia; Hasnawi Hasnawi; I Nyoman Radiarta
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Kabupaten Kepulauan Sangihe dengantiga pulau terdepannya memiliki potensi untuk pengembangan budidaya rumput laut (Kappaphycus alvarezii), namun belum tersedia data kondisi perairannya. Kajian bertujuan untuk mengetahui karakteristik, kesesuaian, dan daya dukung perairan untuk budidaya rumput laut di kawasan pesisir Kabupaten Kepulauan Sangihe. Data kondisi perairan yang dikumpulkan berupa lingkungan fisik dan kualitas air. Analisis dengan weighted linear combinationdalam SIG dilakukan untuk penentuan kesesuaian perairan danbesarnya kapasitas perairan digunakan untuk penentuan daya dukung perairan. Hasil kajian menunjukkan bahwa, karakteristik perairan Kabupaten Kepulauan Sangihe dapat mendukung usaha budidaya rumput laut, namun kedalaman perairan yang relatif dangkal dan adanya alur pelayaran yang menjadi faktor pembatas dalam kesesuaian lahan untuk budidaya rumput laut. Dari 4.839,36 ha kawasan pesisir yang dikaji di Teluk Talengen (Kecamatan Tabukan Tengah), Teluk Manalu (Kecamatan Tabukan Selatan), dan Teluk Dagho dan sekitarnya (Kecamatan Tamako dan Manganitu Selatan) dijumpai kawasan pesisir seluas 181,80 ha yang tergolong sangat sesuai; 852,82 ha yang tergolong cukup sesuai; 3.633,75 ha yang tergolong kurang sesuai; dan 179,99 ha yang tergolong tidak sesuai untuk budidaya rumput laut metoda tali panjang. Budidaya rumput laut metoda tali panjang di Kabupaten Kepulauan Sangihe dapat dilakukan di Teluk Talengen, Teluk Manalu, dan Teluk Dagho dan sekitarnya dengan pengembangan kawasan maksimal masing-masing seluas 324; 559; dan 1.171 ha yang dapat digunakan untuk masing-masing 1.296, 2.236, dan 4.684 unit rakit budidaya rumput laut berukuran 50 x 50 m. Sangihe Archipelago Regency with its three outlying islands has the potential for seaweed farming development (Kappaphycus alvarezii), regrettably reliable water quality data are scarcely available in this region. The study was aimed to determine the characteristics, suitability, and carrying capacity of waters for seaweed farming in the coastal areas of Sangihe Archipelago Regency. The observed environmental quality of coastal waters included physical environment and water quality. A weighted linear combination in a GIS environment method was applied to determine the suitability of waters and the capacity of coastal water to accommodate the maximum surface area of the farm was used to determine the carrying capacity of waters. The results of the study indicated that the characteristics of waters in Sangihe Archipelago Regency provide suitable environment for seaweed culture, however the relatively shallow waters and the existence of the shipping lanes in the study region can become major limiting factors for seaweed culture and development. Of 4,839.36 hectares of the coastal areas studied in Talengen Bay (Tabukan Tengah Subdistrict), Manalu Bay (South Tabukan Subdistrict), and Dagho Bay and surrounding areas (Tamako and Manganitu Selatan Subdistricts), a total of 181.80 ha were classified as very suitable; 852.82 ha were moderately suitable; 3,633.75 ha were less suitable; and 179.99 ha were not suitable for seaweed long-line culture method. Further analysis showed that seaweed culture of long-line method can be effectively practiced in Talengen Bay, Manalu Bay, and Dagho Bay and surrounding areas with the maximum development areas of 324, 559, and 1,171 ha respectively of which can be used for allocating 1,296, 2,236, and 4,684 culture raft units respectively, with the size of 50 x 50 m per unit.

PERKEMBANGAN GONAD DAN PERTUMBUHAN IKAN NILEM BETINA ALL FEMALE HASIL FERTILISASI JANTAN NEOMALE Jojo Subagja; Deni Radona; Anang Hari Kristanto
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Dalam memenuhi kebutuhan produksi diperlukan ketersediaan induk betina yang unggul. Pembentukan all female berasal dari pemijahan jantan fungsional (aplikasi hormon metil testosteron) hasil secara pool gamet dengan betina normal. Penelitian ini bertujuan mengevaluasi perkembangan gonad dan pertumbuhan ikan nilem betina (all female) hasil fertilisasi jantan fungsional (neomale). Pemeliharaan ikan uji all female dilakukan dalam jaring polyetilene berukuran 2 m x 2 m x 2 m dengan kepadatan 60 ekor di Instalasi Penelitian Toksikologi dan Lingkungan Budidaya Cibalagung Bogor. Selama enam bulan pemeliharaan ikan diberi pakan komersil berupa pelet dengan kandungan protein 28% sebanyak 5% dari bobot total dengan frekuensi tiga kali sehari (pagi, siang, dan sore). Pengamatan pertumbuhan dan pengambilan sampel ikan untuk materi histologi dilakukan setiap bulan. Hasil penelitian menunjukkan ikan all female dapat tumbuh dan berkembang normal. Secara statistik pertumbuhan ikan all female berbeda nyata (P<0,05) dibandingkan dengan kontrol, dengan nilai panjang (14,2 ± 2,35 cm), bobot (28,9 ± 2,24 g), dan pertumbuhan bobot relatif (58,79%). Pada perkembangan kematangan gonad ikan all female masuk pada tingkat III dan IV dengan nilai IGS sebesar 20,2% serta tidak ditemukan gonad intersex.In order to supply sufficient seed for grow out industry, the availability of female broodstock takes the priority in hatchery operations. In order to reach that objective, all female seed batch for potential future broodstock could be produced by breeding functional males (induced with methyl testosterone hormone application) with a pool gametes of normal females. This study was aimed to evaluate the gonadal development and growth of all female Bonylip barb produced from fertilization of neomale and normal females. All the females were reared in polyethylene net cages (2 m x 2 m x 2 m in size) with a stocking density of 60 individuals/cage. The trial was conducted at the Cibalagung Toxicology and Environmental Research Station, Bogor. The fish were reared for six months and fed three times daily with commercial pellets (crude protein of 28%) as much as 5% of the total weight. Observations on the growth and fish sample collections for histology analysis were conducted every month. The results showed that all female fish were able to grow and develop normally. Statistically, the growth of all female was significantly different (P<0.05) compared to the control, with the length size of 14.2 ± 2.35 cm and weight of 28.9 ± 2.24 g and relative weight gain of 58.79%. In terms of gonadal development, all female fish reached stage III and IV with IGS value of 20.2% and no inter-sex gonad observed.

Page 1 of 1 | Total Record : 10

Filter by Year

2017 2017

Filter By Issues

All Issue Vol 19, No 2 (2024): Juni (2024) Vol 19, No 1 (2024): (Maret 2024) Vol 18, No 4 (2023): (Desember, 2023) Vol 18, No 3 (2023): (September, 2023) Vol 18, No 2 (2023): (Juni, 2023) Vol 18, No 1 (2023): (Maret 2023) Vol 17, No 4 (2022): (Desember 2022) Vol 17, No 3 (2022): (September) 2022 Vol 17, No 2 (2022): (Juni) 2022 Vol 17, No 1 (2022): (Maret, 2022) Vol 16, No 4 (2021): (Desember, 2021) Vol 16, No 3 (2021): (September, 2021) Vol 16, No 2 (2021): (Juni, 2021) Vol 16, No 1 (2021): (Maret, 2021) Vol 15, No 4 (2020): (Desember, 2020) Vol 15, No 3 (2020): (September, 2020) Vol 15, No 2 (2020): (Juni, 2020) Vol 15, No 1 (2020): (Maret, 2020) Vol 14, No 4 (2019): (Desember, 2019) Vol 14, No 3 (2019): (September, 2019) Vol 14, No 2 (2019): (Juni, 2019) Vol 14, No 1 (2019): (Maret, 2019) Vol 13, No 4 (2018): (Desember 2018) Vol 13, No 3 (2018): (September 2018) Vol 13, No 2 (2018): (Juni, 2018) Vol 13, No 1 (2018): (Maret 2018) Vol 12, No 3 (2017): (September 2017) Vol 12, No 4 (2017): (Desember 2017) Vol 12, No 2 (2017): (Juni 2017) Vol 12, No 1 (2017): (Maret 2017) Vol 11, No 3 (2016): (September 2016) Vol 11, No 4 (2016): (Desember 2016) Vol 11, No 2 (2016): (Juni 2016) Vol 11, No 1 (2016): (Maret 2016) Vol 8, No 3 (2013): (Desember 2013) Vol 5, No 3 (2010): (Desember 2010) Vol 5, No 2 (2010): (Agustus 2010) Vol 5, No 1 (2010): (April 2010) Vol 2, No 2 (2007): (Agustus 2007) Vol 2, No 1 (2007): (April 2007) Vol 1, No 1 (2006): (April 2006) Vol 10, No 4 (2015): (Desember 2015) Vol 10, No 3 (2015): (September 2015) Vol 10, No 2 (2015): (Juni 2015) Vol 10, No 1 (2015): (Maret 2015) Vol 9, No 3 (2014): (Desember 2014) Vol 9, No 2 (2014): (Agustus 2014) Vol 9, No 1 (2014): (April 2014) Vol 8, No 2 (2013): (Agustus 2013) Vol 8, No 1 (2013): (April 2013) Vol 7, No 3 (2012): (Desember 2012) Vol 7, No 2 (2012): (Agustus 2012) Vol 7, No 1 (2012): (April 2012) Vol 6, No 3 (2011): (Desember 2011) Vol 6, No 2 (2011): (Agustus 2011) Vol 6, No 1 (2011): (April 2011) Vol 4, No 3 (2009): (Desember 2009) Vol 4, No 2 (2009): (Agustus 2009) Vol 4, No 1 (2009): (April 2009) Vol 3, No 3 (2008): (Desember 2008) Vol 3, No 2 (2008): (Agustus 2008) Vol 3, No 1 (2008): (April 2008) Vol 2, No 3 (2007): (Desember 2007) Vol 1, No 3 (2006): (Desember 2006) Vol 1, No 2 (2006): (Agustus 2006) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Ilham

Contact Email
Ilham.fishaholic@gmail.com

Phone
+6221-64700928

Journal Mail Official
jra.puslitbangkan@gmail.com

Editorial Address
Gedung Balibang KP II, Lantai 2 Jl. Pasir Putih II, Ancol Timur, Jakarta Utara 14430

Location
Kab. jembrana,

Bali

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ilham

Contact Email Ilham.fishaholic@gmail.com

Phone +6221-64700928

Journal Mail Official jra.puslitbangkan@gmail.com

Editorial Address Gedung Balibang KP II, Lantai 2 Jl. Pasir Putih II, Ancol Timur, Jakarta Utara 14430

Location Kab. jembrana, Bali INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ilham

Contact Email
Ilham.fishaholic@gmail.com

Phone
+6221-64700928

Journal Mail Official
jra.puslitbangkan@gmail.com

Editorial Address
Gedung Balibang KP II, Lantai 2 Jl. Pasir Putih II, Ancol Timur, Jakarta Utara 14430

Location
Kab. jembrana,

Bali

INDONESIA