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GENETIC VARIATION OF HUMPBACK GROUPER (Cromileptes altivelis) ON F1 AND F3 GENERATIONS Sari Budi Moria Sembiring; Tridjoko Tridjoko; Haryanti Haryanti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.469 KB) | DOI: 10.29244/jitkt.v5i1.7755

Abstract

Hatchery and culture technology of Humpback grouper has been developed. However,  sometimes it is still found constraint in sedd production and caused instability of production due to failure by many factors. Breeding program become necessary to provide seed with high quality and good genetic variation. Quantitative characteristic selection is one of breeding program to improve productivity in grouper aquaculture. The aim of this research was to produce good phenotyphic and genotypic quality of Humpback grouper candidate broodstock. Selection of Humpback grouper F1 and F3 was done based on quantitative characteristic (body length and weight) and genotyphic characteristic by using ramdom amplified polymorphism DNA (RAPD) method.  The results of conventional selection were found candidate broodstock of Humpback grouper F1 and F3 with body weight range from 170-210 g and 160-170 g consecutively.  Heterozygosity values of Humpback grouper analized by RAPD of F1 and F3 were 0.7940 and 0.7749 consecutively and it was not significantly different. This value emphasis that population of this F3 Humpback grouper was still good to grow for broodstock. Keywords: genetic variation, humpback grouper, F1, F3.
PERFORMANCE OF GONAD MATURATION AND SPAWNED OF GROUPER BROODSTOCK CROSS BREEDING BETWEEN F-2 AND F-0 Tri Djoko; Haryanti Haryanti; Sari Budi Moria; A Muzaki; I K Wardana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.175 KB) | DOI: 10.29244/jitkt.v6i1.8626

Abstract

In fish grouper escpecially humpback grouper culture, seed and broodstock quality and availability determine the production success. Main supply of wild broodstock still dominates grouper hatchery activities, therefore, the exploitation of the wild broodstock is very high. Efforts to use the supply of cultured broodstock through selection and breeding processes will greatly determine their culture success. The purpose of this experiment was to get seeds from the cross breeding between F-2 and F-0. The research used two (2) concrete tanks with volume of 75 m3 each. Each tank was filled with 20  fishes consisting of 10 fishes of F-2 and 10 fishes of F-0. The experiment was conducted the Institute of Mariculture Research and Development, Gondol, Bali. The result showed a good gonadal development and natural spawning of cross breeding between F-2 and F-0. Total number of eggs (1.320.000) was found the highest in May in tank A, while the highest fertilized eggs of 385.000 were found in June.  The SSCP analyses showed no difference in genetic characters of broodstock from cross-breeding between the female parent F-2 and F-0 male parent. Keywords: Humpback Grouper, gonad, spawned, cross breeding, larvae
Analisis Perubahan Penggunaan Lahan Di Kecamatan Pagelaran Kabupaten Peringsewu Tahun 2013-2018 Haryanti Haryanti; Sugeng Widodo; Dedy Miswar
JPG (Jurnal Penelitian Geografi) Vol 7, No 5 (2019): Jurnal Penelitian Geografi (JPG)
Publisher : JPG (Jurnal Penelitian Geografi)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (922.018 KB)

Abstract

The aim of study was to analyze the change of land area, type of change in the use of land and the  factor causing the change  of  the  use  of  land  in Kecamatan Pagelaran Kabupaten Pringsewu in 2013-2018. The data obtained this research were (1) The area changed is 1.517.70 ha’s (20.94%) and the kind of change in the use of land were forest to moor, plantations to moor, farm to settlement, farm to moor, swamp to moor, moor to settlement and moor to farm. (2) The dominant factor that caused the change of the land used was the social economic factor which included of occupation and the increasing number of population.Penelitian ini bertujuan untuk menganalisis luas lahan yang berubah, jenis perubahan penggunaan lahan dan faktor yang menyebabkan perubahan penggunaan lahan di Kecamatan Pagelaran Kabupaten Pringsewu tahun 2013-2018. Hasil yang diperoleh dalam penelitian ini adalah (1) Luas lahan yang mengalami perubahan  yaitu seluas 1,517,70 ha (20,94%) dan jenis perubahan penggunaan lahan yang berubah adalah hutan ke tegalan, perkebunan ke tegalan, sawah ke pemukiman, sawah ke tubuh air ke tegalan, tegalan ke pemukiman dan tegalan ke sawah. (2) Faktor yang dominan menyebabkan perubahan penggunaan lahan adalah faktor sosial ekonomi yaitu pekerjaan dan bertambahnya jumlah penduduk.Kata  kunci:  faktor  penyebab  perubahan  penggunaan  lahan,  jenis perubahan penggunaan lahan, luas perubahan penggunaan lahan,
PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI Ahmad Muzaki; Sari Budi Moria Sembiring; Ida Komang Wardana; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.364 KB) | DOI: 10.15578/jra.12.2.2017.131-137

Abstract

Pertumbuhan dan sintasan larva dan benih kerapu sunu turunan ketiga (F-3) dari induk turunan kedua (F-2) hasil seleksi telah diamati di Balai Penelitian dan Pengembangan Budidaya Laut, Gondol. Induk F-2 diseleksi menggunakan marka indikator tumbuh cepat PL-03 (alel tunggal berukuran 370 bp). Penelitian ini bertujuan untuk mengevaluasi keragaan pertumbuhan dan sintasan larva dan benih kerapu sunu F-3 serta pewarisan sifat tumbuh cepat dari induk kerapu sunu (F-2) kepada benih kerapu sunu F-3. Pembenihan menggunakan induk kerapu sunu F-2 dengan sifat tumbuh cepat (membawa marka PL-03) dan tanpa sifat tumbuh cepat. Telur dan larva yang dihasilkan dipelihara sebagai turunan F-3 hingga berukuran benih dan dibesarkan selama 10 bulan pemeliharaan. Hasil pengamatan sintasan larva kerapu sunu F-3 dari induk membawa marka PL-03 adalah sebesar 10,6% ± 9,1%; sedangkan larva F-3 dari induk yang tidak membawa sifat tumbuh cepat hanya sebesar 2,7% ± 1,7%. Benih F-3 dari induk membawa marka PL-03 dapat dipanen saat mencapai ukuran 2,5-3,0 cm pada umur 38 ± 2 hari, sedangkan benih F-3 dari induk tanpa marka PL-03 pada umur 40 ± 1 hari. Selanjutnya benih kerapu sunu F-3 dari induk yang mempunyai marka PL-03 yang dipelihara selama 10 bulan memiliki laju pertumbuhan harian lebih baik (3,11%) dibandingkan benih kerapu sunu F-3 tanpa marka PL-03 (3,01%). Sintasan benih kerapu sunu F-3 dengan marka PL-03 tidak berbeda nyata dengan yang tidak mempunyai marka PL-03. Persentase benih F-3 turunan dari induk F-2 dengan sifat tumbuh cepat yang membawa marka PL-03 sebesar 45%.The growth and survival rate of the third generation (F-3) of coral trout grouper larvae and fry from the genetically selected broodstock were observed at the Institute for Mariculture Research and Development, Gondol. The F-2 broodstock were selected using the fast-growing marker indicator, PL-03 (single allele of 370 bp in size). The aim of this study was to determine the growth performance and survival rate of larvae and fry of F-3 coral trout groupers, as well as inheritance of fast growth trait from the F-2 broodstock to the F-3. The eggs and larvae of F-3 produced from F-2 broodstock with fast growth trait (having PL-03 marker) and without fast growth trait were reared to reach fry stage for 10 months. The results showed that the survival rate of the F-3 larvae from broodstock with PL-03 marker was 10.6% ± 9.1%, while F-3 larvae from the broodstock without fast growth trait only reached 2.7% ± 1.7%. Fry (F-3) with PL-03 marker reached the optimum harvest size of 2.5-3.0 cm at 38 ± 2 days old, while the F-3 fry of broodstock without PL-03 marker took relatively longer to reach that size (40 ± 1 days age). The F-3 from the broodstock with PL-03 marker reared for 10 months had better growth rate (3.11%) compared to F-3 fry without PL-03 marker (3.01%). The survival rate of F-3 fry with PL-03 marker was not significantly different to F-3 fry without PL-03 marker. The percentage of F-3 fry from the broodstock of F-2 with fast growth trait was 45%.
The Concept and Implementation of Environment-Based Curriculum Management in Elementary School Efi Tri Astuti; Haryanti Haryanti; Mika Andayani
ISTAWA Vol 6, No 1 (2021): Istawa: Jurnal Pendidikan Islam
Publisher : Postgraduate Program Magister Pendidikan Agama Islam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24269/ijpi.v6i1.4916

Abstract

The curriculum is an important aspect of achieving educational goals. Education not only provides knowledge about the environment but also increases awareness and concern for environmental conditions. This research was conducted with the aim of describing the concept and application of environmentally sound curriculum management, supporting factors, inhibiting factors, and efforts to overcome them. This study uses a qualitative - descriptive approach, with a purposive sampling technique. Data was collected through interviews, documentation and literature study. The results showed that SD Alam Pacitan is a school that implements environmentally friendly curriculum management. That curriculum makes environmental elements the main object in the learning process to help characterize students. There are 4 stages carried out, that's are planning, implementation, monitoring, and evaluation. The inhibiting factors consist of the lack of facilities, infrastructure, and funding. The action to overcome this is the establishment of cooperation with many parties.
Gambaran Pengetahuan Orang Tua Tentang Stimulasi Dini Perkembangan Balita (Usia 12-59 Bulan) Haryanti Haryanti; Mefrie Puspita; Meinarisa Meinarisa
Jurnal Medika Cendikia Vol 8 No 2 (2021): Jurnal Medika Cendikia
Publisher : STIKes Karsa Husada Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/medika.v8i2.159

Abstract

ABSTRACT Background Stimulation of growth and development of toddlers is very important given by parents. If the stimulation is inadequate, the growth and development of children under five will be disrupted. Children aged 12-59 months need stimulation to optimize development. One of the causes of delayed child development is parents' ignorance of the importance of developmental stimulation. This study aims to describe the knowledge of parents about the early stimulation of toddler development (aged 12-59 months). Methods: This research is a quantitative descriptive study. The population in this study were all parents who have toddlers aged 12-59 months in the Kenali Besar Public Health Center working area as of October 2019, totaling 3369 people. The sample in this study were 97 people who were taken by accidental sampling technique. This research was conducted on 3 to 18 August 2020 in the Work Area of ​​the Kenali Besar Community Health Center, Jambi City. The data was collected using a questionnaire sheet and the results of this study were analyzed univariately. Results: The results of the univariate analysis showed that there were 52 (53.6%) respondents who had knowledge about early stimulation of toddler development (aged 12-59 months) in a fairly good category in the Work Area of ​​the Kenali Besar Health Center in Jambi City in 2020. Conclusion: Based on the research results, it can be concluded that parents' knowledge about early stimulation can affect the development of toddlers Keywords: Knowledge, Developmental Stimulation, Toddler
Improving Student Learning Outcomes in Writing Paragraphs through The Medium of Illustrated Stories Haryanti Haryanti
Social, Humanities, and Educational Studies (SHES): Conference Series Vol 4 (2021): Social, Humanities, and Educational Studies (SHEs): Conference Series (Special Issue 1
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.985 KB) | DOI: 10.20961/shes.v4i5.66216

Abstract

Keterampilan menulis merupakan keterampilan yang paling kompleks dibanding dengan keterampilan berbicara maupun keterampilan mendengarkan.Menulis memerlukan proses pemahaman,penguasaan keterampilan berbahasa sekaligus konsep dalam menulis.Kompleksnya keterampilan menulis menjadi perhatian khusus bagi guru dalam pembelajaran.Tantangan untuk membelajarkan keterampilan menulis paragraph kepada peserta didik dengan menghilangkan asumsi bahwa menulis itu kegiatan yang sulit,menjadi pusat pemikiran tersendiri bagi guru.Cerita bergambar menjadi salah satu pilihan guru dalam membelajarkan menulis paragraph.Peserta didik yang lebih tertarik pada hal yang konkrit,diharapkan akan memiliki ketertarikan tersendiri akan media cerita bergambar dalam kegiatan menulis paragaraf.
EVALUATION OF RESISTANCE AND GENE EXPRESSION OF BARRAMUNDI, Lates calcarifer POST-INFECTION OF NERVOUS NECROSIS VIRUS Fitriyah Husnul Khotimah; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati; Ketut Sugama; Ahmad Muzaki; Indah Mastuti; Sari Budi Moria Sembiring; Ketut Mahardika; Harton Arfah; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.97-106

Abstract

The most common problem in barramundi Lates calcarifer seedling production is the high mortality (> 90%) caused by nervous necrosis virus (NNV) infection. This research aims to evaluate the resistance and gene expression of barramundi challenged by NNV. Two populations were used in this study, i.e., Australian, and Situbondo-originated barramundi populations. The immune-related gene expression levels in the liver, head of kidney, and spleen were observed at 48 and 96 hours after post-infection (hpi). Barramundi’s survival and blood parameters were evaluated post-NNV infection. The results showed that the highest survival was revealed in Situbondo’s barramundi (42.0±4.47%) compared to Australian barramundi (20.0±7.07%) and no mortality was observed in the control without NNV infection. The higher survival rate in barramundi from Situbondo was in line with the blood profile. The number of red blood cell from Situbondo barramundi post-NNV infection (ST) at 96 hpi was higher (P<0.05) than Australian barramundi post-NNV infection (AT). The number of white blood cell of ST at 48 hpi was higher (P<0.05) than AT, but started to decrease at 96 hpi in ST barramundi. The total white blood cell in AT barramundi increased from 48 to 192 hpi. TNFα and IL1-β gene expression levels were significantly higher in the liver, head kidney, and spleen of Situbondo compared to Australian barramundi at 48 hpi, while MHCIIα gene expression in Situbondo’s was significantly higher compared to Australian barramundi at 96 hpi. These results indicate the important roles of all the genes in the barramundi’s immune responses against viral infection. Based on the results of the research, Situbondo’s barramundi has the potential to be used as a candidate for generating broodstock of disease-resistant strain.
KUALITAS PELAYANAN PERUSAHAAN UMUM DAERAH AIR MINUM TIRTO NEGORO KABUPATEN SRAGEN Haryanti Haryanti; Winarti Winarti; Joko Pramono
JI@P Vol 12 No 1 (2023): JI@P
Publisher : Master of Public Administration, Universitas Slamet Riyadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/jp.v12i1.8073

Abstract

Perusahaan Umum Daerah Air Minum Tirto Negoro is a Regional Owned Enterprise (BUMD) of the Sragen Regency Government which operates provide services for the provision and distribution of drinking water to the community. But at the age of 43 years, Perumda Sragen still has many problems faced in providing services.The purpose of this research is to find out how the service quality of Perumda Sragen. The researcher used a qualitative descriptive approach and purposive sampling technique. Data collection techniques through observation, interview and documentation.The problems that occurred in Perumda Sragen are the number of pipe leaks due to old age causing dirty and cloudy water; damaged water meters; small water discharge during Lebaran Day due to more usage than normal days; and less thorough PPOB (Payment Point Online Banking) payment counter which causes payment errors from customers.The result of this study indicates that the service from Perumda Sragen in terms of the availability of infrastructure and facilities, reliability, and guarantee are good, but only in terms of empathy is being a note to make improvements in providing services to the customers. Keywords: Service, Quality, Perumda Air Minum Tirto Negoro
PENGARUH MEDIA PEMBELAJARAN DAN PENGUASAAN KOSAKATA TERHADAP KEMAMPUAN BERBICARA BAHASA INGGRIS SISWA Haryanti Haryanti
INFERENCE: Journal of English Language Teaching Vol 2, No 1 (2019): INFERENCE: Journal of English Language Teaching
Publisher : Universitas Indraprasta PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30998/inference.v2i1.5452

Abstract

The study aims to determine: 1) The effect of instructional media and vocabulary mastery towards English speaking ability. 2) The effect of Vocabulary mastery towards English speaking ability. 3) The effect of instructional media on English speaking ability. The affordable population is class X of Uswatun Hasanah Private Vocational School, Mardi Bhakti Private Vocational School, East Jakarta. The sample of this research is 70 respondents in this study. The method used is an experiment. Inference analyses used are ANOVA two-way factorial 2x2. The results of this study show:1) There is no significant effect on instructional media and vocabulary mastery towards English speaking ability. This result is evidenced by the acquisition of sig.0,258 > 0,05 and F observe 1,305. 2) There is a significant effect on vocabulary mastery towards English speaking ability. This result is evidenced by the acquisition of sig.0,0,012< 0,05 and F observe 6,741. 3) There is a significant effect on instructional media towards English speaking ability. This result is evidenced by the acquisition of sig.0,000 < 0,05 and F observe 85,435.Key Words: instructional media; game; vocabulary mastery; speaking skill