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KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Published by Universitas Islam Negeri Sumatera Utara
ISSN : -     EISSN : 25986015     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
KLOROFIL: Jurnal Ilmu Biologi dan Terapan is a journal that published by Major of biology and applied sciences, Departmen Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara Medan, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 9 Documents
 1 
Search results for , issue "Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN" : 9 Documents clear
DETEKSI CEMARAN Salmonella sp. PADA KOMODITAS TUNA, TONGKOL DAN CAKALANG (TTC) YANG DIJUAL DI PASAR INPRES DI DAERAH ISTIMEWA YOGYAKARTA Susi Ratnaningtyas; Dzikri Wahyudi; Devi Wulansari; Wayah Putri Utami
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13622

Abstract

Fish is a nutrition source, mainly protein, which is easily decomposed as a result of bacterial and enzyme activity. Therefor its fish after harvest if it is not done properly will cause the fish to become damaged quickly and experience a decrease in quality because fish is a type of food that is easily subject to biological damage and is susceptible to microbial contamination (Christanti and Azhar, 2019). Incorrect handling will lead to decreased product quality. Cross contamination is one result of poor handling processes, so that the product is contaminated by pathogenic bacteria. One of the pathogenic bacteria, namely Salmonella sp. which needs to be considered for its presence in fishery products, both fresh and frozen (Ijong, Berhimpon and Sumampow, 2015). The purpose of this research is to determine the contamination of Salmonella sp. and the prevalence of contamination in Tuna, Cob and Skipjack (TTC) commodities. Final Practical Work (KPA) will be held from 14 March to 10 June 2022 at the Fish Quarantine Station, Quality Control and Safety of Fishery Products in Yogyakarta, located in Sleman Regency, Yogyakarta. The samples tested were 19 fresh fish products in the Tuna, Cob and Cakalang (TTC) commodities. Microbiological quality testing of fishery products, both fresh and frozen, needs to be carried out in order to guarantee food safety for the community. Test procedure for Salmonella sp. using conventional methods in accordance with SNI 01-2332.2-2006, which includes pre-enrichment, enrichment, isolation of bacteria or selective, observation of typical Salmonella colony morphology, isolation of pure culture of Salmonella and biochemical tests. The test results showed that all fresh fish samples taken from the InPres market in the Yogyakarta area were negative for Salmonella sp. and the prevalence is 0%. The application of good sanitation and hygiene will prevent contamination of pathogenic bacteria and still maintain the cold chain to inhibit enzyme reactions found in the fish's body. Bacterial contamination Salmonella sp. usually found in fishery products, agriculture and food products
MINI REVIEW: PENGEMBANGAN BIOSENSOR KOLORIMETRI BERBASIS AGREGASI NANOPARTIKEL EMAS Hana Verdiani; Dinda Syafira; Husna Nugrahapraja
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14657

Abstract

Gold nanoparticle (AuNP) is a material commonly used in colorimetric detection for biological sensors due to its low production cost, simplicity, and ease use. AuNP is known to generate different colors depending on the particle aggregation level. In the last two decades, changes in AuNP aggregation status have become the basis for developing a sensor to detect analytes in the sample. The solution color changes could be observed without sophisticated equipment, allowing ,more straightforward interpretation of the detection result. This review focuses on developing AuNP aggregation-based colorimetric tests for detecting various biological analytes. It discusses multiple mechanisms, strategies, functionalization, the latest research, and perspectives of AuNP use in colorimetric detection, which could be considered for future biosensor development. The concept of colloidal gold nanoparticle aggregation and stabilization is emphasized in this review. 
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% KLIKA TURI (Sesbania grandiflora L.) TERHADAP Staphyloccous epidermidis DAN Propionibacterium acnes Andi Dian Astriani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13519

Abstract

Acne is a skin disease that occurs due to bacterial infection, usually on the skin surface of the face, neck, chest, and back. The Turi plant is one plant with medicinal properties that can be used as an antibacterial. This study aims to determine the antibacterial activity of 70% ethanol extract of klika turi against Staphylococcus epidermidis and Propionibacterium acnes. Klika turi was extracted by reflux method using 70% ethanol solvent. They were testing the antibacterial activity of 70% ethanol extract against Staphylococcus epidermidis and Propionibacterium acnes using liquid dilution and agar diffusion methods. The results showed that the MIC values were 2.5% against Staphylococcus epidermidis and 5% against Propionibacterium acnes. Activity test with the diffusion method to obtain the diameter of the inhibition zone against Staphylococcus epidermidis at an extract concentration of 2.5%; 5%; 10%; and 20%, which is 7.35 mm; 8.67mm; 11.08 mm and 11.55 mm, while for Propionibacterium acnes at an extract concentration of 5%; 10%; and 20% obtained the diameter of the inhibition zone which is 8.77; 11.32mm; and 12.00mm. The results can be concluded that the 70% ethanol extract of klika Turi has effective antibacterial activity at a concentration of 10% against Staphylococcus epidermidis and Propionibacterium acnes.).
ISOLASI DAN KARAKTERISASI BAKTERI AMILOLITIK DARI SAMPEL AIR PANTAI PARIS TIGARAS Vila Tria Putri; Rasyidah Rasyidah; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.12880

Abstract

Amylolytic bacteria have great genetic diversity with high enzymatic activity and have the expertise to break down starch into simpler materials and degrade organic substances in water. This study aims to determine the variety of types of amylolytic bacteria obtained and to see how the characterization of bacteria is isolated from the waters of Paris Tigaras Beach. Isolation of amylolytic bacteria from the waters of Paris Tigaras Beach was carried out using the spread plate method taken from the last dilution of 10-5. Isolates ST3 03 and ST3 04 showed amylolytic activity by showing a clear zone of 11.8 mm in isolates ST3 03 and 8.2 mm in isolates ST3 04. Characterization of isolates ST3 03 and ST3 04 was observed including Gram-positive bacteria with rod-shaped cells, that can produce catalase enzymes, ferment three types of sugars, and move (motil). From the results of observations of the morphology of bacterial colonies and cells, and biochemical tests, isolates of amylolytic bacteria ST3 03 and ST3 04 belong to the genus Bacillus.
Uji Aktivitas Ekstrak Akar Nipah (Nypa fruticans) Sebagai Antimikroba Staphylococcus aureus ATCC 25923 Dan Candida albicans ATCC 10231 Nia Agustina; Mauritz Pandapotan Marpaung
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14059

Abstract

Nipah fruticans adalah sejenis palem yang mengandung senyawa metabolit sekunder yang berpotensi memiliki berbagai efek biologis, seperti antibakteri dan antijamur. Penelitian ini bertujuan menganalisis ekstrak akar nipah dapat menghambat pertumbuhan Staphylococcus aureus ATCC 25933 dan jamur Candida albicans ATCC 10231. Akar nipah diekstraksi dengan metode maserasi dalam pelarut etanol 96%. Skrining fitokimia dilakukan untuk mengindentifikasi senyawa metabolit sekunder. Uji mikroba dengan metode Difusi Agar dengan konsenterasi ekstrak 10%, 20%, 30%, 40%, 60%, 80%, dan 100%. Kontrol positif untuk uji antibakteri adalah klindamisin dan ketokonazol untuk uji antijamur. Hasil penelitian yaitu adanya metabolit sekunder berupa flavonoid, alkaloid, saponin, steroid, dan terpenoid. Diameter hambat ekstrak pada uji bakteri terdapat pada ekstrak 100%, sedangkan pada uji jamur, tidak ditemukan adanya daya hambat.
Hubungan Kekerabatan Beberapa Varietas Unggul Padi (Oryza sativa L) Terpilih Berdasarkan Karakterisasi Kualitas Biji Kiki Nurmayanti
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13851

Abstract

Persamaan dan perbedaan karakter yang dimiliki tanaman padi dapat digunakan untuk mengetahui hubungan kekerabatan yang dimiliki antarvarietas tanaman padi. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan dan jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi. Penelitian dilaksanakan di Kebun percobaan Balai Besar Penelitian Tanaman Padi Sukamandi. Metode penelitian yang digunakan yaitu metode deskriftif eksperimental dengan perlakuan sebanyak 44 varietas padi, diulang 3 kali. Data hasil pengamatan dianalisis menggunakan sofeware Ntsys 2.02 dan 2.11 dengan metode UPGMA. Hasil penelitian menunjukan beberapa varietas unggul terpilih memiliki koefisien kemiripan 0,41 berdasarkan karakter warna lemma steril, warna kepala putik, panjang biji,  panjang beras pecah kulit, bentuk beras pecah kulit serta warna kulit ari beras dan terbentuk dua kelompok dengan hubungan kekerabatan yang jauh. Jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi memiliki keragaman yang luas dengan nilai 0,59. Pasangan varietas yang memiliki jarak genetik yang luas diduga akan menghasilkan turunan yang memiliki tingkat keragaman genetik  tinggi dan sifat unggul yang lebih dari tetuanya.
INVENTARISASI SPESIES MANGROVE DI KAWASAN PESISIR DESA JENILU KABUPATEN BELU NUSA TENGGARA TIMUR Emilia Juliyanti Bria
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.15378

Abstract

Mangroves are important ecosystems in coastal areas that have a major influence on the survival of the biota in them. This study aims to inventory the existence of mangrove species in the coastal area of Jenilu Village, Kakuluk Mesak District, Belu Regency, East Nusa Tenggara. The method used is the observation method. Data obtained by means of inventory and identification of mangrove species. The results showed that there were 2 mangrove species from the Rhizophoraceae Family, Order Malpighiales, namely Rhizophora apiculata Blum and Rhizophora mucronata Poir. These two species are distinguished based on the characters on their morphological characteristics. The data from this research can become the basic data for species development and subsequent conservation efforts.
Diversitas Burung Di Situ Cihuni Dita Ayu Kurnia Sari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13745

Abstract

Birds are wild animals that are easy to find in almost every vegetated environment. Its habitat can cover various types of ecosystems, ranging from natural ecosystems to artificial ecosystems, one of which is Situ Cihuni. This research was conducted as an initial step that can be used as a guide for conservation efforts and to determine the balance of aquatic ecosystems. The method used is the field survey method, where the data collected includes the types of birds that are in the transect line and the number of individuals of each bird species found. The results showed that 24 species of birds could be found in Situ Cihuni, with a total encounter of 354 individuals. A diversity index (H') value of 2.23 was obtained, which indicated that the diversity level of bird species in Situ Cihuni was in the moderate category. Then the value of the wealth index (R) is 3.92; the bird with the highest IVI value was the Lonchura punctulata (110.793). Insectivorous bird groups dominate the Situ Cihuni area with a percentage of 46%. The vegetation strata in Situ Cihuni that are most used by birds are strata C and E.
Kajian Etnobotani Tanaman Hanjuang (Cordyline fruticosa) pada Wawatjan Babad Sumedang Fauzan Ahmad Wijaya
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14589

Abstract

Wawatjan Babad Sumedang is one of the ancient texts telling about the struggle of Prabu Geusan Ulun in defending and taking responsibility for Sumedang from the Cirebon attack as a result of the love between him and Harisbaya, the empress of Cirebon. In one part of this chronicle, the Hanjuang was planted by a character named Embah Djaja Perkosa. It is the background for the study of the manuscript from an ethnobotanical perspective. This study uses qualitative methods with data collection techniques through interviews, observation, and literature studies. Interviews were conducted in a semi-structured and snowball-sampling manner with community leaders. Furthermore, observation was carried out by visiting the Hanjuang Kutamaya complex in Padasuka Village, North Sumedang District, Sumedang Regency, West Java. While the literature study was performed on related references, especially the Wawatjan Babad Sumedang Manuscript. Hanjuang is mentioned in the text in verses 148, 149, 171, and 172. Hanjuang itself is a siloka of "hayu urang berjuang" or "let's fight" . In the written text, the word ngalakai is a sign that the Hanjuang needs an appropriate environment to live in. This suitable environment is filled with the flourishing of Kutamaya thanks to the existence of the river there. In addition, the process of planting Hanjuang in the events in the script is done by stem cuttings. However, the Hanjuang side as an indicator has not been resolved in this study, so further research is needed on this matter

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