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Biota
Published by Universitas Islam Negeri Mataram
ISSN : 20863586     EISSN : 24608483     DOI : -
Core Subject : Health, Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 5 Documents
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Search results for , issue "Vol 14 No 1 (2021)" : 5 Documents clear
Antimicrobial Resistance and Molecular Identification of Clinical Multi-Drug Resistant Enterobacter Cloacae Siti Nur Fajriah; Maulin Inggraini; Noor Andryan Ilsan; Reza Anindita
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.334

Abstract

Enterobacter cloacae is a Gram-negative bacteria causing nosocomial infections. This bacteria has increased resistance to various antibiotics in the past five years, resulting in a multi-drug-resistant (MDR) phenotype. In particular, MDR E. cloacae causes longer hospitalization time, increases medical costs, and affects morbidity and mortality. This study aimed to observe the minimum inhibitory concentration (MIC) of clinical E. cloacae towards several antibiotics and molecular identification of MDR E.cloacae. This study was conducted in a descriptive design. Secondary data was collected at the microbiology laboratory of the Teaching Hospital in Bekasi, Indonesia, from May to September 2020. Sampel was carbapenem resistant E.cloacae. The isolate was originated from a human clinical specimen, then was confirmed molecular identification using 16s rRNA. In this study, only one carbapenem-resistant E. cloacae, which is also MDR bacteria, was found. This E. cloacae was categorized as MDR bacteria since it was resistant to more than three antibiotic classes, including carbanemen, extended-spectrum cephalosporin, penicillins + β lactamase inhibitor, antipseudomonal penicillins + β lactamase inhibitor aminoglycoside, and penicillin. Vitek 2 identification of this isolate was E. cloacae complex. It showed similar results to molecular identification based on a partial sequence of 16s rRNA. BLASTn result of the trimmed sequence was E. cloacae with 99.78 % similarity.
pengaruh kombinasi ekstrak benalu teh dan mangga terhadap profil histopatologi tikus hipertensi (DOCA-SALT) Nimas Ajeng Oktaviana; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.339

Abstract

The purpose of this study was to analyze the brain histopathological profile in hypertensive rats (DOCA-Salt) treated to Tea and Mango extract combination (Mistletoe). Samples were obtained using a true experimental design completely randomized on 25 rats with three treatments (TI, TII, TIII), negative control, and positive control with replication five times. Ratio of extract for treatment was 3 : 1 made up 50 mg/kg BW, 100 mg/kg BW, 200 mg/kg BW. Data analysis using one-way analysis of variance (ANOVA) with the JAMOVI application. The study results obtained Mistletoe (Tea and Mango) extract combination in hypertensive rats (DOCA-Salt) can significantly reduce the necrosis of brain cells in the white matter area. The combination of Mistletoe extract can reduce the number of brain cell necrosis in hypertensive rats optimally obtained at a dose of 50 mg/kg BW
Kelimpahan Mikroba Tanah Asal Rizosfer Tanaman Sukulen Aidha Zulaika; Dian Rachma Wijayanti; Wulan Fitriani Safari
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.340

Abstract

Plant host symbiosis is close related to soil microbial abundance. Soil microbial abundance will affect soil quality and fertility, thus will improve plant growth. Studies on soil microbial abundance in succulent plant rhizosphere, especially in Indonesia, are scarce. This study aims to observe soil microorganisms' existence and their abundance on succulent plant rhizosphere. This research used two primary methods to isolate Arbuscule Mycorrhizal Fungi (AMF) and actinomycetes. The spore extraction (soil separating) method was used to obtain AMF spores, followed by the root staining method to observe AMF infection on the plant roots. Serial dilution and pour plate method were used for isolation of Actinomycetes. The isolation results showed a high number of Actinomycetes distribution up to 3.3 x 106 Actinomycetes CFU/g from the plant Echinocactus grusonii, while AMF spores displayed the most elevated number up to 47 spore/25g from the plant Deuterocohnia sp. The percentage of AMF root infection is covered by 27,9% median. AMF identification was based on spore. The result showed an abundance presentation of Actinomycetes in each soil sample. AMF and Actinomycetes existence displayed symbiotic interaction between succulent plants and soil microorganisms. AMF and actinomycetes play the role of endophytes that help the growth of cacti, generally have a dry growing environment, and limited nutrition by this symbiotic interaction. Actinomycetes distribution in rhizospheric soil will increase plant growth-promoting factors activity. Eventually, the implications of research results are to explore the abundance and biodiversity of soil microorganisms from succulent plant rhizosphere that lacked exploration.
Polyploidy Induction of Rutaceae through Bio-catharanthine Treatment Isna Rasdianah Aziz; Cut Muthiadin; Hajrah Hajrah; Rahmat Fajrin Alir; Fanny Dhea Suryafly; Alifah Zul Amnah; Isna Ainun Hermawan; Muhammad Khalifah Mustami; Mahfut Mahfut; Brij Mohan Upreti
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.344

Abstract

Indonesia has a tropical monsoon-type climate which suitable for agriculture and plantations. One of the main centers of orange fruits (Citrus sp.) production in Indonesia and become the top ten with the largest production is in South Sulawesi. Farmers commonly cultivate siam oranges (Citrus nobilis). Lime (Citrus aurantifolia) has not yet become the main production, which is inversely proportional to the high demand in South Sulawesi. This study aims to determine the ability of bio-catharanthine as a polyploidy agent against the phenotype of the familia Rutaceae cultivated in South Sulawesi. The research was carried out in Laboratory of Genetics and Molecular Biology, Department of Biology, Universitas Islam Negeri Alauddin Makassar using randomized complete block design consisted of two conditions (bio-catharanthine concentration including C1 = Bio-catharanthine 0.05%; C2 = Bio-catharanthine 0.075%; C3 = Bio-catharanthine 0.1% and immersion time including T1 = 3 h; T2 = 6 h). Phenotypic observations include plant height, the number of leaves and nodes, the length of roots, and the number of roots per shoot. The results of the study showed that the bio-catharanthine immersion of 0.05-0.075% for 6 hours in Rutaceae had a significant effect on their phenotypic characters.
Comparison of the Chaetodontidae Mitochondrial Genome Rhavy Ferdyan; Abdul Razak; Yuni Ahda
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.341

Abstract

The mitochondrial genome is useful for identification because the mutation rate of mtDNA is higher than nDNA. Previous studies have carried out many analyses on the mitochondrial genome of several species, the Chaetodontidae group. Fish in this group are biological indicator fish of coral reefs, so their molecular characteristics must be enriched to conserve these fish. This article aims to review several forms of the mitochondrial genome of the Chaetodontidae group of species, including: Chaetodon speculum, Chaetodon octofasciatus, Chaetodon wiebeli, and Heniochus chrysostomus. Data were collected qualitatively by reviewing previous research articles related to the Chaetodontidae mitochondrial genome. The review focused on the characteristics of each species' mitochondrial genome, including length, overall composition, tRNA, and non-coding region. Each species exhibits various features of the mitochondrial genome and is mapped in its phylogenetic analysis.

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