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Ervina Julien Sitanggang
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Nommensen Journal of Medicine
Published by Universitas HKBP Nommensen
ISSN : 24601616     EISSN : 26862565     DOI : https://doi.org/10.36655/njm.v5i2
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health
Nommensen Journal of Medicine (NJM) memuat artikel penelitian (research article), artikel review, dan laporan kasus (case report) di bidang kedokteran.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 72 Documents
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Unmethylated Vitamin D Receptor (VDR) pada Multi Drug Resistant Tuberculosis Christine Verawaty Sibuea; Janry Sinaga; Elsa Bea Nibasi Zega; Restin Julianti Zebua; Selvian Batee
Nommensen Journal of Medicine Vol 9 No 1 (2023): Nommensen Journal of Medicine: Edisi Agustus
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i1.1203

Abstract

ABSTRACT Background : Cases of Tuberculosis (TB) and multi-drug resistant Tuberculosis (MDR TB) caused by mycobacterium tuberculosis are high globally. Vitamin D has an important role against TB infection by inducing antimicrobial peptides such as cathelicidin through vitamin D receptors (VDR). Several studies suggest that there is a genetic influence for TB infection, such as polymorphism and methylation. Methylation of VDR gene is one of the causes of susceptibility to TB infection. Objective : This study aimed to describe the unmethylated vitamin D receptor gene in patients with MDR TB and pulmonary non-MDR TB in Medan City. Methods : This research was a descriptive studied with a cross sectional research design. Stored DNA sample isolated from blood 40 patient MDR TB blood and 40 patient non MDR TB was converted to bisulfite with EZ DNA Methylation-Gold Kit. Converted DNA was amplified by PCR and finally gel electrophoresis was performed with agarose gel to see the unmethylated. Results : Unmethylated VDR gene was higher in MDR TB (17,5%) than in non MDR TB (null). Unmethylated VDR gene was found higher in men (15%) and in age 18-49 (10%). Conclusion : There was no unmethylated VDR gene in non MDR TB. The unmethylated VDR gene was found in MDR TB. Keywords : MDR TB, VDR gene, unmethylated ABSTRAK Latar belakang : Tuberkulosis (TB) dan multi drug resistant tuberculosis (MDR TB) yang disebabkan oleh mycobacterium tuberculosis sangat tinggi kasusnya secara global. Vitamin D mempunyai peran penting melawan infeksi TB dengan menginduksi peptida antimikroba seperti cathelicidin melalui vitamin D receptor (VDR). Beberapa penelitian menyebutkan adanya pengaruh genetik dalam terinfeksi TB paru yaitu polimorfisme dan metilasi. Metilasi gen VDR menjadi salah satu penyebab kerentanan terinfeksi TB paru. Tujuan : Penelitian ini bertujuan untuk mengetahui gambaran unmethylated gen VDR pada penderita MDR TB dan non MDR TB di Kota Medan. Metode : Penelitian ini merupakan penelitian deskriptif kategori dengan rancangan penelitian cross sectional. Sampel DNA tersimpan yang diisolasi dari darah 40 penderita MDR dan 40 penderita non MDR TB dikonversi menjadi bisulfit dengan EZ DNA Methylation-Gold Kit. DNA yang terkonversi diamplifikasi dengan PCR dilakukan elektroforesis gel dengan gel agarosa untuk melihat metilasi. Hasil : Unmethylated gen VDR lebih banyak pada MDR TB (15%) daripada non MDR TB (0). Unmethylated gen VDR lebih banyak ditemukan pada laki-laki (15%) dan pada usia 18-49 tahun (10%). Kesimpulan : Tidak terdapat unmethylated gen VDR pada non MDR TB. Unmethylated gen VDR ditemukan pada MDR TB. Kata Kunci : MDR TB, gen VDR, unmethylated
Gambaran Profil Hematologi Rutin Pasien TB Paru Sebelum dan Sesudah Pengobatan dengan Obat Antituberkulosis Ervina Julien Sitanggang; Jenny Ria Sihombing
Nommensen Journal of Medicine Vol 9 No 1 (2023): Nommensen Journal of Medicine: Edisi Agustus
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i1.1263

Abstract

Background: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (M.tb) which often attacks the lung parenchyma and can also attack other organs. Changes can be found in the hematological profile of TB patients both at the time of diagnosis and after treatment. Objective: This study was conducted to obtain routine hematological profiles of pulmonary TB patients before and after treatment with anti-tuberculosis drugs for 3 months, 4 months, and 5 months. Methods: This study was an observational descriptive study. The population in this study was pulmonary TB patients at Martha Friska Hospital and Hermina Medan Hospital for the period October 2021-August 2022. The samples in this study were taken from medical record data of all pulmonary TB patients at Martha Friska Hospital and Hermina Medan Hospital for the period of October 2021-August 2022 (total sampling). Results: In this study, 39 research subjects were obtained, age range of 15-79 years. Before treatment, the majority of patients had anemia (76.92%), leukocytosis (61.54%), normal platelets (66.67%), and elevated ESR (53.85%). After 3 months of treatment, anemia was experienced by 30%, leukocytosis by 0%, normal platelets by 80%, and elevated ESR by 20% of patients. After 4 months of treatment, anemia was experienced by 52.17%, leukocytosis by 4.35%, normal platelets by 65.22%, and elevated ESR by 4.35% of patients. After 5 months of treatment, anemia was experienced by 50%, leukocytosis by 0%, platelets normal by 83.33%, and elevated ESR by 0% of patients. Conclusion: The majority of patients develop anemia, leukocytosis, and elevated ESR before treatment with antituberculosis drugs. There was a decrease in the proportion of patients who developed anemia, leukocytosis, and an elevated ESR after treatment with antituberculosis drugs for 3 months, 4 months, and 5 months.

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