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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 6 Documents
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Search results for , issue "Vol. 3 No. 1 (2021)" : 6 Documents clear
Distribution Patterns of Exotic Plant Chromolaena odorata, in Rehabilitation Zone at Donglo Block, Resort of Wonoasri, Meru Betiri National Park Dwi Wardatul Rizkiah; Hari Sulistiyowati; Arif Mohammad Siddiq
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p1-6

Abstract

Exotic plants are plants that are introduced intentionally or unintentionally from their original habitat to a new habitat. One of the exotic plants that is potentially invasive is Chromolaena odorata. These plants are found a lot especially in the rehabilitation zone at Donglo Block Resort of Wonoasri, Meru Betiri National Park (MBNP). An initial effort to determine whether this exotic plant was potentially invasive was to use the distribution pattern of a plant population. This study aimed to determine the distribution pattern and area of cover of exotic plants C. odorata in rehabilitation zone at Donglo Block, Resort of Wonoasri MBNP. The sampling of C. odorata used the transect plot method systematically, which carried out in Donglo Block Resort of Wonoasri MBNP. Data analyzed using Morisita index. The map of distribution pattern of C. odorata was created using the Geographic Information System (GIS). Based on the results of the Morisita index, the value (Iδ) = 12.39, which means that C. odorata has a clumped distribution pattern. The distribution pattern of C. odorata can also be seen from the visualization of the spatial distribution map, which shows that the growth of C. odorata in plants or overlaps with each other, hence that it looks clustered. The coverage area of C. odorata in the study location was 596,5 m2 or 29.24% of the total area of the study (20,400 m2). Based on the results of the coverage area of C. odorata, it was indicated that this exotic species is not yet classified as an invasive species in the area.
The First Investigation of Microplastics Contamination in Estuarine Located in Puger District, Jember Regency, Indonesia Selvi Ariyunita; Yeny Dhokhikah; Wachju Subchan
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p7-12

Abstract

Microplastics are harmful to the ecosystem and need to solve immediately. Detection of microplastic contamination is the first step to reduce plastics pollution. Estuarine in Puger has a high potential for microplastic pollution related to the multifunction of waters as ports, tourism, fish market, fish processing, and human settlements. However, there are no studies related to microplastic contamination in the area. The objectives of this research were to determine microplastic contamination in the estuarine located in Puger District, Jember Regency, Indonesia. The research results were the first evidence to inform the society and local government about the actual condition of plastic pollution, especially in the aquatic ecosystem. The sampling sites were determined by purposive sampling. Fifty liters of water from each station (with three repetitions) were taken using a 24V water pump and then filtered using stainless steel filter (mesh 5 mm and 0.2 mm). The filtered samples were placed in a sterile bottle sample, stored at 4 ± 20C. Microplastics were counted and categorized according to size, color, and type under a microscope stereo. Microplastic abundance was calculated based on the number of microplastic particles identified per liter of sample water (particle/liter). The result showed that the study area was contaminated by microplastics throughout site sampling, with abundances varying from 0.03 particles/liter to 0.19 particles/liter. The highest microplastic abundance found near the fishery market. The microplastics also vary in size, color, and type. According to characterization, the sources of microplastic contamination come from human-based activities.
Design of the env-su Gene Coding for Surface Unit Protein as a Vaccine Candidate for Jembrana Disease Virus in In Silico Nur Asih Setiarini; Indriawati Indriawati; R Susanti Susanti; Endang Tri Margawati
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p13-22

Abstract

Bali cattle are superior meat producers, but they are susceptible to Jembrana disease. The injection of the crude vaccine was considered ineffective, so the env-su gene was selected to express the Jembrana Surface Unit (JSU) protein as a candidate for the Jembrana vaccine. This study aimed to analyze the potential of the JSU protein as a candidate for the Jembrana vaccine and analyze the increase in the env-su gene expression which codon has been optimized in silico. Vaccine design was carried out through in silico including the selection of the SU protein sequences of the genus Lentivirus and sequence alignment of UniProt. The construction phylogeny tree of SU protein using MEGA-X program, optimization of env-su gene codon with preference codon Esherichia coli str. K-12 substr. MG1655 using Optimizer. The optimized env-su gene was inserted into the plasmid pET-21a (+) using GenScript. The result of sequence alignment showed that there is no SU protein that has a percent identity value of more than 30% with JSU protein. The SU JDV and BIV proteins are monophyletic groups and have a percent identity of 20.57%. Codon optimization showed an increase in CAI by 1,000 and GC 54.5%, and a decrease in ENc to 22 and AT 45.5%. EcoR1 and HindIII can recognize the gene target and MCS cut regions on the plasmid so that the env-su gene can be inserted into the pET-21a (+) plasmid. The JSU protein has the potential to be a candidate for the Jembrana vaccine, but it needs further research in vitro and in vivo.
Screening of Extracellular Enzymes on Serratia marcescens strain MBC1 Meishy Handerlin Putri; Kusuma Handayani; Wawan A Setiawan; Berliana Damayanti; Cindy Lukyta Ratih; Achmad Arifiyanto
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p23-29

Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities havent been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.
Diversity and Community Structure of Dragonfly and Damselfly (Odonata) at the Selorejo Waterfall Area, Ponorogo Regency, East Java Indonesia Muhamad Azmi Dwi Susanto; Siti Zulaikha
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p30-37

Abstract

Selorejo Waterfall is a natural tourism area that is quite far from downtown Ponorogo and directly adjacent to the Gunung Sigogor Nature Reserve. Hence, this area has the potential as a natural habitat for dragonfly and damselfly. The presence of dragonfly and damselfly species is determined by the type of habitat, canopy conditions, vegetation diversity, and the microclimate that exists in a location. This study aimed to determine the diversity of dragonfly and damselfly and to determine the community structure of dragonflies in Selorejo Waterfall. The sampling method was Visual Day Flying. The results of research conducted in two streams showed that there were 12 species from 6 families with a total of 230 individuals. The value of species diversity at this location is H '= 2.05. In the community structure at the Selorejo Waterfall dragonfly, there are differences in the number of species and individuals in the two streams in Selorejo Waterfall Area. The large stream, eight species from four families, 151 individuals in total. Meanwhile, in small stream, there were nine species from six families, 79 individuals in total. The differences in the number of species and individual dragonflies in the two streams at Selorejo Waterfall can be used to describe the diversity and structure of the Odonata community in the area.
The Comparison of SARS-CoV-2, SARS-CoV, and MERS-CoV Genome and Spike Protein Variations Choirun Nita Fikriani; I Kade Karisma Gita Ardana; Dwi Listyorini
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p38-44

Abstract

SARS-CoV-2 is a virus that has caused COVID-19 pandemic. This virus is a new variant of the SARS-CoV virus and also closely related to MERS-CoV, which caused similar acute respiratory infections. All these viruses recognize target cells by binding to the Receptor Binding Domain (RBD) on Spike protein with receptors. This study aimed to determine the SARS-CoV-2, MERS-CoV, and SARS-CoV genome structure, Spike protein sequence differences, and variations of RBDs Receptor Binding Motif (RBM). This research was using data mining approach. Genome sequences were downloaded from NCBI, except for Indonesian samples were downloaded from GISAID. Genomic structures, Spike sequence, and RBD structure were analyzed using Bioedit, followed by protein modelling using SwissModel and PyMol applications. The result showed that the SARS-CoV-2, MERS-CoV, and SARS-CoV genome shared the same genes yet in different numbers and length. SARS-CoV-2 Spike protein sequence was quite similar to SARS-CoV Spike protein, but very different to the Spike protein of MERS-CoV. There were variations of RBDs RBM structure due to the mutations occurred among these viruses. It is suggested that these differences may increase the affinity between SARS-CoV-2 Spike protein to its hACE2 receptor which caused SARS-CoV-2 becomes more infective and spread wider than SARS-CoV and MERS-CoV, in turn. This result expected to be basic information for the development of SARS-CoV-2 introduction inhibition agent and spreading prevention.

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