cover
Contact Name
Dwi Anggorowati Rahayu
Contact Email
dwirahayu@unesa.ac.id
Phone
+6281235407983
Journal Mail Official
jrba@unesa.ac.id
Editorial Address
Department of Biology Faculty of Mathematics and Natural Sciences Universitas Negeri Surabaya Jalan Ketintang Gedung C3 Lantai 2 Surabaya 60231
Location
Kota surabaya,
Jawa timur
INDONESIA
Jurnal Riset Biologi dan Aplikasinya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Articles 6 Documents
Search results for , issue "Vol. 3 No. 2 (2021)" : 6 Documents clear
Diversity of Lepidoptera: Rhopalocera at Selorejo Waterfall Area, Ponorogo District, East Java, Indonesia Siti Zulaikha; Muhamad Azmi Dwi Susanto
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p68-72

Abstract

Selorejo Waterfall is a natural tourist area that is directly adjacent to the Sigogor Mountain Nature Reserve and has a beautiful and natural environment. This makes the Selorejo Waterfall area has a good environment for the sustainability of various species of butterflies. This research aimed to find out the diversity and abundance of butterflies in the Selorejo Waterfall area. This research was conducted using the Visual Day Flaying method by following the trail in the Selorejo Waterfall area. This study found 26 species belongs 4 families with 109 individuals in total. The value of the species diversity index at the location of H'= 2.81 moderate category. The diversity of butterflies in the Selorejo Waterfall area was influenced by the availability of host plants and feed as well as abiotic factors.
S-D614G Mutation Reveals the Euro-America and East-Asia Origin SARS-CoV-2 Virus Spread in Indonesia Nina Bunga Anggraini; Dwi Listyorini
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p45-53

Abstract

COVID-19 is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The first case was found in the city of Wuhan, Hubei province, China. The first case in Indonesia was reported in March 2020 and currently there are 0.5 million cases with a death rate of 3.1%. This rapid increase in cases is thought to due to presence of the mutant strain S-D614G, which causes a faster rate of infection and spread. The purpose of this study was to determine the presence of S-D614G mutations in Indonesian samples in order to find the origin of COVID-19 which was spread in Indonesia based on the Spike gene sequences and the RdRp genes from 25 countries, and one control sequence China/Wuhan-Hu-1 obtained from the NCBI and GISAID databases. Mutation analysis was carried out through multiple alignments using BioEdit software. Phylogenetic tree reconstruction using MEGA6 software with the Neighbor Joining method. This study found mutation of S-D614G in one Indonesian sample, namely the Indonesian/SBY9 sample along with 23 samples from Europe, America, and Africa. The phylogenetic tree reconstruction confirmed these findings; the mutated samples were closely related to samples from these continents, while the non-mutated Indonesian samples were closely related to sample from East Asia. These findings indicate that the origin of the SARS-CoV-2 virus in Indonesia possibly came from the East Asia cluster and the European-American cluster.
Diversity of Butterflies (Hexapoda: Lepidoptera: Rhopalocera) Around Campus 4 of Universitas Ahmad Dahlan Ichsan luqmana Indra Putra; Tasya Aulia Putri; Haris Setiawan
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p54-62

Abstract

The conversion of land functions can result in a decrease of environmental quality, which can reduce the diversity of butterflies. This study aimed to determine the diversity level and to find the most abundant and less abundant butterfly species around campus 4 UAD. The sampling was done at campus area, vacant land, rice fields and housing sites. Each of these sites had 2 plots and consisted of 5 subplots, respectively. Sampling was carried out four times, which was done in the morning at 08.00 - 11.00 and in the afternoon at 15.00 - 17.00. Butterflies caught using a sweep net. The data were analyzed by inferential analysis, namely Pearson correlation test. The calculation of the diversity level of butterflies was carried out by calculating the importance value index, the diversity Shannon-Wiener index, and the Simpson dominance index. The results showed that the diversity level of Shannon Wiener index of butterflies around the campus 4 UAD showed a low level (0.67). The species of butterflies found around campus 4 UAD were Acraea violae, Aphrissa statira, Appias libythea, Appias olferna, Catopsilia pyranthe, Catopsilia Scylla, Danaus Chrysippus, Elymnias hypermnestra, Junonia almana, and Junonia atlites. The most abundant butterfly was C. pyranthe with 125 individuals whereas the least abundant was A. libythea with 1 individual. This suggested that the ecosystem around campus 4 UAD is disturbed category; causing instability of the diversity that exists, including butterflies.
The population of Sanderling (Calidris alba) in 2020 Migration on the South Coast of Puger, Getem, Jember Abdu Rohman; Ragil Satriyo Gumilang
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p63-67

Abstract

Sanderling (Calidris alba) is a type of shorebird that migrates from the northern hemisphere to the southern hemisphere. Indonesia is a migration route for East Asia-Australasia. The southern coastal area of the Jember Regency, one of the wetlands, especially in East Java, has become an air route and a stopover for shorebirds every year. The research objective was to determine the population of Sanderling (Calidris alba) on the southern coast of Puger and Getem, Jember Regency. Method of collecting encounter rates. Bird watching is carried out in the morning at 06.00-08.00 and in the afternoon at 15.30-17.30. Data recording includes species, number of birds, and other supporting data. Identification using the book by Mac Kinnon et al. (2010) and Bhushan, B., et al. (1993). Tools used for binocular observation, monocular, camera, and GPS. Data analysis used the density index for individual bird species using the formula method (Alikodra, 1990). The results of the study were a total of 445 individuals. Data analysis of Muara Sungai population density was the location with the four other survey locations' highest number.
Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata) Chumaidatul Choiriyah, S.Si; Nirmala Fitria Firdhausi; Esti Tyastirin; Yuanita Rachmawati; Moch. Irfan Hadi
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p80-87

Abstract

Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.
Variation and Phenetic Relationship of Tobacco (Nicotiana tabacum L.) In Central Java and Yogyakarta Based on Morphological Characters Agung Dwi Santoso; Purnomo Purnomo
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p73-79

Abstract

Tobacco (Nicotiana tabacum L.) is a plant used as a mixture of cigarettes, and recreational media especially for men. This study aimed to identify variations, and determine the relationship between tobacco cultivars in Central Java and Yogyakarta based on macromorphological and micromorphological characters. Sampling locations are determined by surveying locations in both regions. Tobacco samples found include 5 cultivars in Central Java namely 'Mantili', 'Uler Magetan', 'Garut', ‘Gober Boyolali’, 'Manila', and 3 cultivars in Yogyakarta namely 'Siluk', 'Java', and 'Virginia'. Characterization with 23 qualitative macromorphological characters including leaves, and stems, with 9 qualitative and quantitative micromorphological characters including trichome and stomata. Descriptive data analysis is done to obtain the typical character of each cultivar, followed by numerical analysis including scoring characters processed with MVSP (Multi Variate Statistical Package), clustering with UPGMA (Unweighted Pair Group Method with Averages), and calculation of similarity coefficients with Simple matching formula. The results showed variations in the macromorphological characters including the shape of the leaf lamina, the base of the leaf, the absence of leaf stalks, and type of leaf venation. Tobacco has anisositic stomata, and varies in terms of length, width, and density of stomata. Tobacco trichomes are glandular. The result dendrograms form two clusters (A and B) with the similarity index of each cluster above 0.80. Cultivars with close relationships such as 'Siluk'-'Java', and far relationship like 'Java'-'Manila'.

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