cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Anna Safitri
Contact Email
jsmartech@ub.ac.id
Phone
+6281235367181
Journal Mail Official
jsmartech@ub.ac.id
Editorial Address
Research Center for Smart Molecule of Natural Genetics Resources (SMONAGENES) office: 2nd floor MIPA Building, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Jl. Veteran Malang, East Java, Indonesia – 65145
Location
Kota malang,
Jawa timur
INDONESIA
JSMARTech : Journal of Smart Bioprospecting and Technology
Published by Universitas Brawijaya
ISSN : 26860805     EISSN : 27147894     DOI : https://doi.org/10.21776/ub.jsmartech.2020.001.02.
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience
JSMARTech : Journal of Smart Bioprospecting and Technology (p-ISSN: 2686-0805, e-ISSN : 2714-7894) is an Open Access Scientific Journal published by Research Center of Smart Molecule and Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Malang, East Java, Indonesia, since 2019. It is a journal covering of bioprospecting, biochemical, biotechnology, bioinformatics, natural product, pharmaceuticals, biomedical, genetics engineering, nutrigenomic, and nanotechnology. The journal publishes a manuscript written in English for original research papers, short communications, and review articles. The paper published in this journal implies that the work described has not been, and will not be published elsewhere, except in abstract, as part of a lecture, review or academic thesis.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 54 Documents
 2   3   4   5   6 
Front Matter JSMartech Vol.03., No.02 Fatchiyah, M.Kes., Ph.D
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 3 No. 2 (2022): JSMARTech Volume 3, No. 2, 2022
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2022.003.02.35

Abstract

Retinal Ganglion Cell Death of Guinea Pig is Slower Than Rat as Optic Nerve Transection Animal Model Tomohiko Sasase; Fatchiyah Fatchiyah; Takeshi Ohta
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.04

Abstract

Glaucoma is a progressive optic neuropathy that leads to retinal ganglion cell (RGC) apoptosis and is a leading cause of acquired blindness. Recently, lack of clinical protective treatment for  optic nerve in glaucoma thus inhibitor drugs towards progression of RGC degeneration are urgently needed. In this study, we established a guinea pig optic nerve transection model and investigated the conditions under which RGC death is evaluated in the development of retinal neuroprotective drugs for the treatment of glaucoma. Guinea pig optic nerve axotomy induces RGC death similarly to other rodent models; however, the time to progress to significant RGC death is 21 days after transection and is longer than in the rat, 10 days after optic nerve axotomy. The results are useful for evaluating species-specific optic neuroprotective agents against rats and mice.
Antioxidant Assay of Endophytic Fungi Extract from Mango Mistletoe (Dendrophthoe pentandra (L.) Miq) Leaves Dewi Qurrot 'Aini; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.09

Abstract

Endophytic fungi found in mango mistletoe (Dendrophthoe pentandra (L.) Miq) leaves may potentially create a variety of bioactive antioxidant compounds, and their prospects for large-scale production especially for phytopharmaceuticals products without excessive natural exploitation. The objective of this study was to investigate the antioxidant properties of endophytic fungal extracts obtained from mango mistletoe leaves. The method used is experimental laboratory research with a quantitative approach through the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity method. The test sample concentrations ranged from 120 µg/ml to 200 µg/ml. The results of this study indicated that isolates of EFMM 1 (Aspergillus spp.) has an IC50 of -12,27 µg/ml, EFMM 2 (Neurospora spp.) has an IC50 of 10,40 µg/ml, EFMM 3 (Hormiscium spp.) has an IC50 of 49,44 µg/ml, and EFMM 4 (Exserohillum spp.) has an IC50 of 299,32 µg/ml. The conclusion is that EFMM 1 doesn’t have an antioxidant activity, while EFMM 2 has the highest antioxidant potential comparable to ascorbic acid.
Secondary Metabolite Composition and Bioactivity of Genus Albizia: A Review Momodou Salieu Sowe; Anayo Christian Etonihu
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.14

Abstract

Traditional medicine is being revalued all around the world because of considerable research on various plant species and their therapeutic properties. Traditional medicinal beliefs and practices are deeply rooted in The Gambia, and forest resources are a source of plants for medicine. A study has estimated the presence of one hundred and fifty (150) plant species in The Gambia, distributed between 28 different plant families. Among the plant species, Albizia seems to possess numerous pharmacological properties. Albizia plants are high in secondary metabolites, according to phytochemical investigations, and pharmacological tests of plant extracts have demonstrated antibacterial, anti-inflammatory, and analgesic effects. Albizia plant components are used in traditional medicine to cure a variety of diseases like lung infections, asthma, pile, diarrhea, gonorrhea, ulcers, hemorrhoids, anxiety, depression, and insomnia. Different solvent extracts of the genus Albizia have demonstrated excellent efficacy against S. Typhimurium, S. Enteritidis, S. Dublin, S. aureus, E. coli, V. parahaemolyticus, B. cereus, S. aureus, and S. pyogenes. The radical scavenging activity of the genus Albizia exhibits a significant antioxidant activity with relatively high EC50 and IC50 values.
In Vitro Screening of Antibacterial Activity of Endophytic Mold Extract from Mango Mistletoe Leaves (Dendrophthoe pentandra (L.) miq) Nafisa Nafisa; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.59

Abstract

Endophytic microbes live in plant tissues and can be extracted to obtain active compounds. One conserved endophytic microbes is endophytic mold, found in plant tissue systems, such as leaves, flowers, branches, and plant roots. The endophytic mold was obtained from the mango parasite (Dendrophthoe pentandra (L.) Miq), a parasitic plant that contains secondary metabolites. This study aimed to determine the antibacterial properties of the endophytic mold isolates extract from mango parasite leaves against Escherichia coli. This research used an experimental method with descriptive quantitative data analysis. The research began with the purification of endophytic mold isolates, production of bioactive compounds, extraction of bioactive compounds, and determination of antibacterial activity by disc diffusion method. The results obtained from this study included four isolates containing antibacterial compounds, including isolates DBM 1, DBM 2, DBM 3, and DBM 4. Extracts of the endophytic mold of mango parasite leaves showed quite good inhibition of the growth of Escherichia coli, with the results of measuring the diameter of the largest inhibition zone of 13.17 mm, which was produced by isolate DBM 1 at a concentration of 75 ppm. Based on the results of antibacterial activity tests on endophytic molds from the leaves of the mango parasite (Dendrophtoe pentandra (L.) Miq), isolates of endophytic fungi can be used as antibacterial agents in the medium renewable category.
Phylogenetic construction of Green Algae Based on the rbcL Gene Aliarani Rosyidiana Putri; Galuh Arkana; Haikal Ageng Maulana; Septhyanti Aprilia Kavitarna; Rayhan Yugo Nurul Maulana; Muhammad Wisam Wira Sakti; Turhadi Turhadi; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.25

Abstract

Chlorophyta (green algae) is one of the algae that can be found in (both oceans and freshwater lakes) as well as land with temperate to dry climates. Chlorophyta or green algae can be found in oceans, freshwater lakes, temperate land, and dry climate land.  In addition, chlorophyta can also be found in semi-aquatic environments, such as rocks, moist soil, and moist tree bark. This study aims to analyze the phylogenetic relationship among chlorophyta species based on rbcL gene to classify the diversity of green algae in Indonesia. The green algae gene rbcL sequence with 20 species was obtained from NCBI. The sequences obtained were aligned with ClustalW and the mutation patterns were analyzed. The aligned sequences were made phylogenetic trees by MEGA-X with the Maximum Parsimony method and 1000 bootstrap. The total percentage of transversions that occurred was 10.75%, while the percentage of transitions was 7.85%. The construction of the phylogenetic tree shows that the chlorophyta species are grouped based on their habitat in the same clade. Clad 1 consists of genera Chlorella, Coccomyxa, and Volvox with freshwater habitats and a genus of Parachlorella with soil habitat. Clade 2 consists of genera Chlorococcum, Tetradesmus, Crucigenia, and Desmodesmus with freshwater habitats and a genus of Chlorococcum with soil habitats. Clad 3 consists of genera Ulva and Chlorella salina with marine habitat. The furthest pairwise distance analysis results were shown by Coccomyxa subellipsoidea and Sargassum schnetteri with a value of 1.18902. The kinship of the species of the Chlorophyta division based on the rbcL gene is divided into 3 clade separated by habitat. The phylogenetic tree in the Chlorophyta division shows that the rbcL gene is sensitive enough to separate among genera and can be utilized in phylogenetic studies.
Phylogenetic Analysis of UreABC Protein in Ureolytic Bacteria as Self-Healing Agent in Concrete Amalia Danti Safa Audia; Else Vilia Setiawan; Giral Baines Mora; Grace Christy; Nabila Shafa Yumna Salsabila; Firda Nuri Asyhari; Turhadi Turhadi; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.49

Abstract

Concrete is one of Indonesia's most widely used materials in infrastructure development because it is strong enough to withstand pressure, adaptable, and has relatively low maintenance costs. Bacteria are potential agents that can be used to close cracks in concrete. Therefore, this research is aimed to determine which bacteria can produce the enzyme urease based on constructing a phylogenetic tree, the ability to produce spores, and the characteristics of ureolytic bacteria. This study used four sequences of ureolytic bacteria that code for ureABC, namely Bacillus, Clostridium, Pseudomonas, and Enterobacter, with Micrococcus terreus as the outgroup. The four bacterial groups with outgroups were aligned and phylogeny constructed using the Neighbor-Joining (NJ) method with 1000 replication and grouped based on genetic distance in MEGA-X software. Then further screening was carried out based on the ability to form endospores and the characteristics of ureolytic bacteria. Phylogenetic analysis shows that the bacterial groups Bacillus, Enterobacter, Clostridium and Pseudomonas have a fairly close kinship, the construction is based on proteins, genus, cell shape, gram characteristics, and habitat. The ureolytic bacteria group predicted to have the highest potential as a biomaterial agent comes from Bacillus sp. and Clostridium sp. due to its ability to form endospores. Ureolytic activity is indicated by an increased pH value and urea degradation activity due to ammonification with Bacillus sp. having a pH of 6.0-8.0. In contrast, the pH value of Clostridium sp. is unknown because further research is needed in vitro.
A Virtual Inhibition of Anti-diabetic Activity of Bioactive Compounds in Harp (Sandoricum koetjape (Burm. f.) Merr.) Elvina Rashida Khairi; Rahmi Izzati; Raissa Kendra Ainiyah; Salma Wahid Marseti; Nuraini Rosyadah; Turhadi Turhadi; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.41

Abstract

The prevalence of type 2 diabetes mellitus (DMT2) has increased worldwide over the last 3 decades. This prompted the researchers to consider the medical benefits of plants, including harp (Sandoricum koetjape). In Indonesia, harp is a common medicinal herb used to cure fever, colic, vaginal discharge, diarrhea, and bloating. harp also contains substances like flavonoids, tannins, or quinones that have anti-diabetic properties. This study aims to determine the potential compound from the harp plant in inhibiting the breakdown of sugar using molecular docking to minimize the buildup of sugar in the blood. Bioactive compounds of harp were obtained from earlier studies and collected from KNApSAcK database. The physicochemical properties of the compounds were analyzed by using SwissADME and PASS Online. Using the CB-Dock server and the receptors α-amylase and α-glucosidase retrieved from RCSB PDB, along with acarbose as a control, the selected compounds were examined for their molecular interactions and binding affinities. Molecular interactions were visualized using Biovia Discovery Studio 2019. The result showed that three compounds were discovered to show potential as antidiabetic medicines out of the 54 active compounds that were screened using SwissADME. Those three compounds are sandorinic acid A, sandorinic acid B, and quercetin with the absolute value of free binding energy ranging from -7.2 to -9.6 kcal/mol. By targeting residues found in amylase and glucosidase, sandorinic acid A, sandorinic acid B, and quercetin have binding sites that are similar to acarbose. The three bioactive compounds quercetin, sandorinic acid a, and sandorinic acid b that are found in harp are expected to have the potential to be agents of type 2 diabetes mellitus.
Virtual Prediction of The Potency of Common Jasmine (Jasminum officinale L.) Bioactive Compounds as Anti-Aging Agents for Natural Sunscreen Fathiyah Nurul Izzah; Dawama Nur Fadlilah; Yohanna Sisilia Grace Sirait; Nandagesta Aurelia Shafa Wagmi; Abdullah Abdullah; Turhadi Turhadi; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 3 No. 2 (2022): JSMARTech Volume 3, No. 2, 2022
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2022.003.02.73

Abstract

UV exposure causes protein denaturation in the skin, leading to wrinkles and photoaging. Matrix metalloproteinases (MMPs) are enzymes that degrade the matrix and are associated with destruction processes like skin aging. Tyrosinase is the rate-limiting enzyme in the melanin synthesis process. We predict that Jasminum officinale L. bioactive compounds can be used as inhibitors for MMP1, MMP3, and tyrosinase. This study aims to compare the potential of bioactive compounds in Jasminum officinale L. as an inhibitor for the MMP1, MMP3, and tyrosinase receptors to prevent anti-aging. Vanillin, hydroxytyrosol, salicylic acid, 3,4-dihydroxy benzoic acid, oleoside-11-methyl ester, ursolic acid, oleanolic acid, and jasminine were retrieved from PubChem database as ligands, to dock with MMP1, MMP3, and tyrosinase as receptors that were retrieved from RCSB PDB. ADMET analysis was performed using pKCSM. Target-based virtual screening was performed using AutoDock Vina in PyRx. Visualization and interactions of amino acid residues was performed using Biovia Discovery Studio 2021. The complex structure was analyzed by molecular dynamics using the CABS-Flex 2.0 website. The oleoside-11-methyl ester compound has the highest potential as an MMP1 inhibitor, the 3,4-dihydroxy benzoic acid compound has the potential to inhibit MMP3, and hydroxytyrosol has the potential to inhibit tyrosinase involved in skin aging because it has low toxicity, highest binding affinity, and low fluctuations based on RMSF values in molecular dynamics simulations. Thus, this study indicated that bioactive compounds in common jasmine (Jasminum officinale L.) can be used as a candidate for organic sunscreen.
Inhibition of Antibacterial Bioactive Compounds from Awar-Awar (Ficus septica Burm, F.) Leaves for Methicillin Resistant Staphylococcus aureus (MRSA) Infection: an In Silico Study Friska Amanda Nurlailiyah; Ni Putu Alira Kinanti Febrina Putri; Siti Saidah Qurrotul A’yun; Sofia Nur Cahyani; Aneila Maritza Larasati; Victor Alvianoes Guterez Hose; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.32

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is a type of Staphylococcus aureus infection that can increase the patients’ number with prevalence variation in each country. Methicillin resistant Staphylococcus aureus (MRSA) can be treated by inhibiting the activity of the BlaR1 transducer protein as an MRSA sensor against transmembrane antibiotics using bioactive compounds. Ficus septica is a local variety that has potential as an antibacterial. In silico study of the bioactive compounds found in Ficus septica can inhibit the synthesis of Staphylococcus aureus cell wall based on molecular docking tests with the control compound is Linezolid as antibiotics. In addition, ADME prediction, binding affinity, and binding site analysis were also performed. The results showed that there were 9 active compounds from Ficus septica leaves which had antibacterial potential and fulfilled Lipinski's rules criteria. Analysis of the binding site indicated that Coumarin compound could bind to the most BlaR1 protein active sites than other bioactive compounds in Ficus septica, including on the Lys526 and Thr527 active sites. Based on interaction analysis, it can be concluded that the Coumarin compounds of Ficus septica leaves can provide a therapeutic effect that can fight MRSA infection which can be proven by in vitro tests.

Page 5 of 6 | Total Record : 54

 2   3   4   5   6 

Filter by Year

2019 2023


Reset
Filter By Issues
All Issue Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023 Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023 Vol. 3 No. 2 (2022): JSMARTech Volume 3, No. 2, 2022 Vol 2, No 3 (2021) Vol 2, No 2 (2021) Vol 2, No 1 (2020) Vol 1, No 2 (2020) Vol 1, No 1 (2019)