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HAYATI Journal of Biosciences

hayati
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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 9 Documents

Search results for , issue "Vol. 19 No. 3 (2012): September 2012" : 9 Documents clear

Properties of Folate Binding Protein Purified from Cow’s Milk . SUBANDRATE; DWIRINI RETNO GUNARTI; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after b-mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.

Cycad Aulacaspis Scale, a Newly Introduced Insect Pest in Indonesia RANGASWAMY MUNIAPPAN; GILLIAN W. WATSON; GREGORY ALLYN EVANS; AUNU RAUF; NATALIA VON ELLENRIEDER
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Cycad aulacaspis scale (Aulacaspis yasumatsui Takagi (Hemiptera: Diaspididae)) is native to Thailand and Vietnam. Since the early 1990s it has been spreading around the world due to the trade in cycad plants for ornamental use. Infestation by this scale can kill cycads in only a few months. Its accidental introduction to Florida endangered the ornamental cycad-growing industry; and in Guam and Taiwan, endemic cycads (Cycas micronesica and C. taitungensis, respectively) are currently threatened with extinction by cycad aulacaspis scale. In November 2011, an introduced scale was discovered damaging cycads in the Bogor Botanic Garden. Samples from Bogor were taken for identification of the scale, and the material was kept for some time to rear out any insect parasitoids that were present. Both the scale insects and parasitoids were prepared on microscope slides and studied microscopically for authoritative identification. The scale was confirmed as A. yasumatsui. The parasitoid Arrhenophagus chionaspidis Aurivillius (Hymenoptera: Encyrtidae) and the hyperparasitoid Signiphora bifasciata Ashmead (Hymenoptera: Signiphoridae) were identified from the samples. Unless immediate remedial measures are taken, several endemic species of cycad in Indonesia may be endangered by infestation by cycad aulacaspis scale.

Cohabitation Study of the Leaf Monkey and Bornean White-Bearded Gibbons in Gunung Palung National Park, West Kalimantan YANTO SANTOSA; . TAQIUDDIN; ABDUL HARIS MUSTARI; DEDE AULIA RAHMAN
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Diet and habitat overlaps were studied for the leaf monkey (Presbytis rubicunda) and bornean white-bearded gibbons (Hylobates albibarbis) in tropical forest of Cabang Panti Research Station (CPRS), Gunung Palung National Park, West Kalimantan. Systematic data on feeding and ranging behaviour were collected from August 2009 up to February 2010 for the three groups of two sympatric primate species that shared two neighbouring patches. Our results showed that seven types of habitat in CPRS were affected to both primates, particularly in plant utilization for feed and the use of vertical space patterns. If the leaf monkeys were present in the same forest patch, the Bornean white-bearded gibbons showed a reduced within-group dispersal and significantly less foraging time in a given forest patch. This might be due to the bornean white-bearded gibbons were more selective in their diet selection. When fruits were scarce, bornean white-bearded gibbons spent most of their foraging time in many types of forest ecosystem, while leaf monkey foraged within one or two types of forest ecosystem. At this period, diet and habitat overlaps between the two species were low. When the availability of fruits increased, leaf monkeys shifted their foraging range and both species became confined to the forest habitat. Consequently, the overlaps of diets and habitats were increased while the peak was at the end of the fruit season.

Association of Sexual Maturation and Body Size of Arfak Children ELDA IRMA JEANNE JOICE KAWULUR; BAMBANG SURYOBROTO; SRI BUDIARTI; ALEX HARTANA
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Gonad maturation in pubertal girls and boys is accompanied with somatic growth spurt, changes in quantity and distribution of body fat (BF), development of secondary sex characters, and relevant physiological events. Menarche (first event of menstruation) and spermarche (first event of nocturnal sperm emission) are usually used as indicators of gonad maturation. We found that median age at menarche of Arfak girls in Manokwari, West Papua is 12.2 years, while median age at spermarche of boys is 13.6 years. A possible factor causing young age at menarche is due to adaptation to unstable environmental conditions because of high risk of mortality by malaria disease during childhood. The events of menarche and spermarche achieved one year after the peak body height (BH) velocity, and just before or at the same time with the time of maximum growth rate of body weight (BW), body mass index (BMI), and BF. The average BMI of Arfak girls was big at 21.9 kg/m2 at the time of their menarche. Bigger average BMI might be caused by prepubertal slowing down of BH growth compare to growth of BW whichis still increasing. Girls accumulate BF before puberty to be used as an energy reserve for the occurrence of menarche. At the time of development of secondary sexual characters girls use the fat reserve so it decline sharply after puberty. In boys, growth rate of BF was stopped at 11 years old, and then growing negatively presumably because boys use fat mass for the occurence of spemarche. BF growth rate reached the lowest point at the age 16 years old, and then increase linearly with age through adolescence until adulthood at age 23 years old.

Phage FR38 Treatment on Sprague Dawley Rat Inferred from Blood Parameters and Organ Systems DEWI SARTIKA; SRI BUDIARTI; MIRNAWATI SUDARWANTO
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

The ability of phage FR38 to lysis indigenous Salmonella P38 from feces of diarrheal patient has been studied. However, effects of phage FR38 on organ system were not revealed as yet. This study was conducted to observe the effect of phage FR38 on blood chemistry, kidney functions, and liver functions. Twelve Sprague-Dawley rats were used as a model for this study that were divided into two groups; (i) control and (ii) treated group with phage FR38. For treated phage group, each rat was administered by 5 ml/kg bw of 1.59·107 pfu/ml of phage intragastric. The blood parameters were analysed on day 16. The results revealed that body and organs weight, erythrocyte, hematocrit, hemoglobin, leukocyte, total protein, creatinine, SGOT, and SGPT of phage treatment rats were not significantly different with the control rats on day 16 (P > 0.05). Therefore, this study showed was no effect of phage FR38 on body weight, blood chemistry, kidney and liver functions of the rat (P > 0.05).

Zingiber officinale, Piper retrofractum and Combination Induced Apoptosis and p53 Expression in Myeloma and WiDr Cell Lines HENY EKOWATI; ANISYAH ACHMAD; EKA PRASASTI; HENDRI WASITO; KADEK SRI; ZULIA HIDAYATI; TUTI EKASARI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

In previous studies, Zingiber officinale, Piper retrofractum, and the combination showed cytotoxic activity, induced apoptosis, and p53 expression of HeLa, T47D, and MCF-7 cell lines. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale (ZO), Piper retrofractum (PR), and the combination as well as their effect to p53 expression on Myeloma and WiDr cells. The powder of ZO, PR, and ZO + PR combination (1:1) were macerated with 96% ethanol for 3 x 24 hours. MTT cytotoxic assay was performed on Myeloma and WiDr cell lines. Apoptotic cells were stained with ethidium bromide and acridine orange. Imunohistochemical expression of p53 was examined on Myeloma and WiDr cell lines. Doxorubicin was used as positive control in all assays. Results showed that ZO, PR, and ZO + PR combination had cytotoxic activity on Myeloma cells with IC50 of 28, 36, and 55 mg/ml respectively and WiDr cell lines with IC50 of 74, 158, and 64 mg/ml respectively, induced apoptotic activity, and increased p53 expression on Myeloma and WiDr cells. These results suggest that ZO, PR, and their combination induced Myeloma and WiDr cells in apoptosis through p53 expression.

Genetic Diversity of Acacia mangium Seed Orchard in Wonogiri Indonesia Using Microsatellite Markers VIVI YUSKIANTI; KEIYA ISODA
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Genetic diversity is important in tree improvement programs. To evaluate levels of genetic diversity of first generation Acacia mangium seedling seed orchard in Wonogiri, Central Java, Indonesia, three populations from each region of Papua New Guinea (PNG) and Queensland, Australia (QLD) were selected and analyzed using 25 microsatellite markers. Statistical analysis showed that PNG populations have higher number of detected alleles and level of genetic diversity than QLD populations. This study provides a basic information about the genetic background of the populations used in the development of an A. mangium seed orchard in Indonesia.

Yeast Isolation for Bioethanol Production EKA RURIANI; TITI CANDRA SUNARTI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.

Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories SRI HANDAYANI; IMAN SANTOSO; HANS-JOACHIM FREISLEBEN; HARALD HUBER; . ANDI; FERY ARDIANSYAH; CENMI MULYANTO; ZESSINDA LUTHFA; ROSARI SALEH; SERUNI KUSUMA UDYANINGSIH FREISLEBEN; SEPTELIA INAWATI WANANDI; MICHAEL THOMM
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.

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