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Titania T Nugroho
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INDONESIA
Jurnal Natur Indonesia
Published by Universitas Riau
ISSN : 14109379     EISSN : 25030345     DOI : http://dx.doi.org/10.31258/jnat
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 18, No 1 (2020)" : 5 Documents clear
Transepidermal Water Loss Value Comparison Between Tengkawang and Durian Seed Oil Lotion Febriaty, Irma Ramadhani; Usman, Thamrin; Alimuddin, Andi Hairil
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (860.444 KB) | DOI: 10.31258/jnat.18.1.20-30

Abstract

Body epidermis has an important health function to prevent body dehydration. This research aims to compare Transepidermal Water Loss (TEWL) values between durian seed oil and tengkawang oil. Skin that had been applied with tengkawang oil and durian seed oil lotion were tested for irritation and TEWL values. The tengkawang oil had an acid number of 17 g/mol and contained  8.54 % free fatty acid, while the durian seed oil had an acid number of 6.34 g/mol and contained 2.89 % free fatty acid.  Irritation testing was done using lotion containing 100 % of each oil. TEWL analysis on skin applied with the tengkawang oil lotion after 1 hour showed that the lotion could reduce epidermal water evaporation by 35.23 % compared to the skin condition before treatment.  Epidermal water evaporation reduction at the second, third and fourth hours after the tengkawang oil lotion application was 31.33 %, 33.93 % and 27.9 %, respectively. TEWL analysis on skin applied with the durian seed oil lotion after 1 hour showed that the lotion could reduce epidermal water evaporation by 11.34 % compared to the skin condition before treatment.  Epidermal water evaporation was reduced 14.64 %, 10.53 % and 2.99 % at the second, third and fourth hours after application of the durian seed oil. Based on these results, it can be concluded that tengkawang oil gives better TEWL values compared to durian seed oil.
Fractionation of Garcinia Mangostana Fruit Pericarp Cellulase Assisted Extracts by Preparative Thin Layer Chromatography and High Performance Liquid Chromatography Nugroho, Titania Tjandrawati; Puja, Krisna; Eryanti, Yum; Miranti, Miranti
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.602 KB) | DOI: 10.31258/jnat.18.1.31-42

Abstract

The polar extract of Mangosteen (Garcinia mangostana) fruit pericarp obtained by cellulase assisted ethanol extraction has strong antioxidant activity, giving an average 2,2 diphenyl-1 pykrylhydrazyl (DPPH) radical  scavenging IC50 of 13.9 µg/mL. In order to elucidate the chemical component from this extract that is responsible for the high antioxidant activity, fractionation of the extract should firstly be performed. In this paper we show results of preparative fractionation of the polar extract by two methods, namely preparative Thin Layer Chromatography (PTLC) and preparative High Performance Liquid Chromatography (PHPLC). PTLC used Silica Gel G60 plates, with a hexane:ethyl acetate (6:4) eluent. PHPLC was a reverse phase method, using C18 column and water:acetonitrile gradient elution. 4 fractions from PTLC and 6 fractions from PHPLC were collected and their antioxidant activity analyzed. Both methods gave separated fractions with lower antioxidant activity than the unfractionated original crude extracts, showing that the strong antioxidant activity of Mangosteen pericarp polar extracts maybe due to the concerted synergetic effect of several compounds, rather than a single isolated compound. It also shows the high degree of difficulty in separating mangosteen pericarp polar components having antioxidant activity for further structural analysis.
Isolation, Characterization and Antibacterial Activities of Lactic Acid Bacteria Isolated From Batak's Special Food "Dali Ni Horbo” Nasution, Muhammad Hasan Basri; Ramadhani, Shafira; Fachrial, Edy
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.779 KB) | DOI: 10.31258/jnat.18.1.1-11

Abstract

Lactic Acid Bacteria (LAB) are gram-positive bacteria that are rod or round in shape, do not form spores, and do not have cytochromes. LAB can be isolated from fermented food products. LAB is useful to inhibit the growth of pathogenic bacteria so that they are potential antibacterial sources. The purpose of this study was to isolate LAB from the traditional food Dali Ni Horbo, continued with characterization, antimicrobial testing, and sensitivity testing towards several antibiotics. From the results of the study, the number of colonies obtained was 2.4 x 107 CFU/mL. 6 isolates were randomly selected, coded as isolates UPDH1, UPDH2, UPDH3, UPDH4, UPDH5, UPDH6, respectively. Biochemical characterization showed that the isolates were gram-positive and catalase-negative bacteria.  Results of antibiotic sensitivity testing using 6 antibiotics, namely amoxicillin (AML), erythromycin (E), oxacillin (OX), ofloxacin (OFX), cefotaxime (CTX), and gentamicin (CN), showed that all the isolates were resistant to CTX. The UPDH1 isolate was resistant to AML and OX. The UPDH3, UPDH5, and UPDH6 isolates were resistant to AML, and the UPDH4 isolate was resistant to E, OFX, CTX, CN, and OX. Antimicrobial tests used the disk diffusion method against Escherichia coli and Staphylococcus aureus. The UPDH2 isolate, gave an inhibition zone of 6.7 mm, and UPDH5 and UPDH6 gave inhibition zones of 7.6 mm and 8.5 mm respectively, against E. coli. Microbial inhibition tests against S. aureus by UPDH1, UPDH2, UPDH5 and UPDH6 gave inhibition zones of 13.5 mm, 9.0 mm, 12.1 mm and 12 mm respectively. From the results in this research it can be concluded that the traditional food Dali Ni Horbo is a potential source of probiotics.
Efficacy of Cellulolytic Bacteria Consortium for Composting Oil Palm Empty Bunches Containing Phytonutrients Aini, Diah Nurul; Linda, Tetty Marta
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.212 KB) | DOI: 10.31258/jnat.18.1.12-19

Abstract

Oil palm empty fruit bunches (OPEFB) are themost solid palm oil waste. OPEFB has often been processed into compost with the addition of certain activators. It is expected that with the addition of a consortium bioactivator composting of OPEFB can be faster and the compost produced has good nutrient content. The study aims was to determine the ability of bioactivator bacteria of cellulolytic consortium in degrading TKKS of incubation laboratory scale for 30 days. A consortium of compost bioactivator used were Bacillus sp. S43, Bacillus cereus strains of IARI-MB-6, Bacillus cereus strains TS11, Alcaligenes faecalis strains ZJUTBX11, Bacillus sp. 13847, Stenotrophomonas sp. S169-III-5, Alcaligenes faecalis strains KH-48 and Bacillus cereus strain Y22 by a comparison of 1:1:1:1:1:1:1:1. The results showed that bioactivator consortium was able to degrade OPEFB which on P4 (OPEFB + chicken manure + consortium isolate) reduced organic C from 50.1 to 34.5, increased total nitrogen from 0.73 to 1.35 and reduced the C/N ratio from 37.11 to 25.56 and produced compost phytonutrients and not phytotoxicity.
The Productivity of Duck in Different Temperature Cage Management Nova, Tertia Delia; Syahruddin, Erman; Zein, Rijal
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.919 KB) | DOI: 10.31258/jnat.18.1.43-61

Abstract

This study aims to determine the performance of  ducks on the effect of differences in cage temperature. This study used 54 5-week-old male ducks selected from 66 ducks. The research method used was randomized block design (RBD). The treatment used is the regulation of the temperature of the cage which is divided into three temperature levels, namely: A (cold temperature), B (room temperature), and C (hot temperature). The observed variables were duck performance in the form of feed consumption, body weight gain and feed conversion. The results of this study indicate that the difference in temperature of the cage became a very significant (P<0.01) on the consumption of rations, weight gain and conversion. The results of the study concluded that the maintenance of ducks at three different cage temperatures had a very significant (P<0.01) on feed consumption and body weight gain, but had no effect on feed conversion. The best results were found in the temperature of the cold cage with the consumption of rations of 2964.33 g/head, which resulted in body weight of 711.83 g/head of g/head, and conversion of 4.2. While the body weight of 1437 percentage of carcass was 67.64% in the study. The highest yield IOFC results (income over feed cost) cage in cold temperatures with a profit of Rp. 3,375.15/head. The maintenance of  ducks at three different cage temperatures had no significant effect (P>0.05) on the weight of the spleen, thyroid and kidney and the work of the spleen, thyroid and kidney organs was still normal

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