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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : http://dx.doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 232 Documents

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Extraction of Chemical Warfare Agents from Soils: Case Study on O-ethyl S-2-(diisopropylamino)ethyl methylphosphonothiolate (VX) Zuas, Oman
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Extraction of chemical warfare agents, O-ethyl S-2-(diisopropylamino)ethyl methylphosphonothiolate (VX) in soilsample has been carried out. The extraction was performed using six different solvents including 1% TEA/MeOH,1% NH4OH/MeOH, water pH 2 at the ambient temperature , water pH 2 at temperature 4 0C, water pH 7 at ambienttemperature, and water pH 7 at temperature 4 0C. Percent recovery of VX in soil samples was quantitativelydetermined by mean LC-MS using selected reaction monitoring (SRM). Among the solvents used, water pH 2 attemperature 40C gave the best extraction capability that was indicated by the highest percent recovery of VXobtained. Storing effect of spiked samples was also investigated by extracting the samples containing VX usingwater at pH 2/40C and the degradation product was then identified using multiple reaction monitoring (MRM). Fromthe study, two degradation products were identified as Bis[2-(diisopropylamino)ethyl]disulphide and ethylmethylphosphonate.

Terjadinya Isomerisasi dan Oksidasi - dan -Karoten Selama Proses Pengolahan CPO Kusumaningtyas, Reni Subawati; Martosupono, artanto
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Isomerization and oxidation has been known as the major factors of the degradation of carotenoid compounds.The carotenoid compounds in oil palm fruit are dominated by - and -karoten. Result of this experiment showedthat the isomerization of the - and -karoten during the process productions of crude palm oil (CPO) indicated bythe reducing of trans ? and trans -caroten concentrations and followed by the increasing of cis - and cis ?-caroten concentrations. Isomerization promote to increasing the concentration of cis - and cis -carotenapproximately 40 ppm and 19 ppm on the sterilization process, then 79 ppm and 134 ppm on the extraction andclarification process of crude oil. On the other hand, the purification process just only increased the concentrationof cis ?-karoten about 19 ppm. The Oxidation of the - and -caroten caused to the increasing of lutein andzeaxantin as the degradation product. The - and -caroten oxidation can be increasing the amount of lutein andzeaxantin approximately 1 ppm and 5 ppm on the sterile fruit, 22 ppm and 14 ppm on the extract crude oil, 1 ppmand 3 ppm on purifying oil. The oxidation process of oil palm extract in the vacuum drier tank can be increased thelutein concentration about 6 ppm.

Isolasi dan Identifikasi Komponen Utama Minyak Atsiri dari Kulit Buah Jeruk Manis (Citrus sinensis L.) Asal Timor, Nusa Tenggara Timur Siburian, Rikson
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Isolation and identifications of peel orange oils (Citrus sinensis, L.) which maserased by lard, coconut oil and mixof lard-coconut oil ( 2 : 1) has been done. This research aimed to obtain main component of peel orange oils fromTimor, knowing influence of lard, coconut oil and mix lard-coconut oils whom using as maserate to results ofchemistry components and atsiri’s oil rendemen product. In this research has been done by using maseration,extraction and using FT – IR and GC – MS as characterizations instruments. The results showed D- Limonen as amain component of atsiri oil from peel orange’s Timor.

Komunitas Parasitoid Lalat Pengorok Daun pada Pertanaman Sayuran Dataran Tinggi Rustam, Rusli; Rauf, Aunu; Maryana, Nina; Pudjianto, Pudjianto; Dadang, Dadang
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Leafminer fly, Liriomyza spp. are widely known as new important pests attacking many vegetable species inhigh land in Indonesia. The objective of the research was to study community of parasitoid leafminer in highaltitude, and parasitization level. This research also looked at more specific on parasitization level caused byOpius chromatomyiae. There were four species of leafminer recorded from the survey, Liriomyza huidobrensis,Liriomyza sativae, Liriomyza chinensis and Chromatomyia horticola. The observation also found eight species ofparasitioid associated with collected leafminers. Six species of recorded parasitoid belonged to family of Eulopidae,and two rest species belonged to Braconidae and Eucoilidae respectively. Those eight species of parasitoids wereHemiptarsenus varicornis, Asecodes deluchii, Neochrysocharis okazakii, Neochrysocharis formosa ,Neocrhysocharis sp. Quadrastichus liriomyzae, Opius chromatomyaie, Gronotoma micromorpha. ParasitoidOpius chromatomyiae and Hemiptarsenus varicornis were found to be dominant in the range altitude between1001-1300 and 1301-1600 asl. From 16 spesies of hosts attacked by leaf miners, only Opius chromatomyiae wasrecorded to have ability to parasitize the leafminer in 13 host species with parasitization level reached 1,84–62,26%.

Statistical Significance Test for Neural Network Classification Rezeki, Sri; Subanar, Subanar; Guritno, Suryo
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Model selection in neural networks can be guided by statistical procedures, such as hypothesis tests, informationcriteria and cross validation. Taking a statistical perspective is especially important for nonparametric models likeneural networks, because the reason for applying them is the lack of knowledge about an adequate functionalform. Many researchers have developed model selection strategies for neural networks which are based onstatistical concepts. In this paper, we focused on the model evaluation by implementing statistical significancetest. We used Wald-test to evaluate the relevance of parameters in the networks for classification problem.Parameters with no significance influence on any of the network outputs have to be removed. In general, theresults show that Wald-test work properly to determine significance of each weight from the selected model. Anempirical study by using Iris data yields all parameters in the network are significance, except bias at the firstoutput neuron.

Pemarasitan Benalu Dendrophthoe pentandra (L.) Miq. pada Tanaman Koleksi Kebun Raya Cibodas, Jawa Barat Sunaryo, Sunaryo
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The mistletoes of Dendrophthoe pentandra (Loranthaceae) grow as parasite on several plant collections of CibodasBotanical Gardens. Plant collections which attacked by mistletoe are Ficus type (Moraceae), and by individual innumber at most is Syzygium racemosum (Myrtaceae). 299 infected and uninfected branches of 67 host specieswere observed. The results showed that the host branches can be destroyed by mistletoe. Moreover, in the naturalconditions, the mistletoe causes degraded of the distal part of the infected branches.

Isolasi dan Karakterisasi Inulinase dari Aspergillus niger Gmn11.1 Galur Lokal Saryono, Saryono
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Inulin is a naturally potential polysaccharide used to produced fructose and fructooligosaccharide. Inulinaseknown also as ß-fructosidase can hydrolise inulin to fructose or fructooligosaccharide. Inulinase production fromAspergillus niger Gmn11.1 isolated from dahlia tubers is conducted using medium containing 1% inulin and 0,2%yeast extract. The crude enzyme (filtrate culture) is purified by means of ammonium sulphate salt precipitation,followed by Sephadex G25 gel filtration column chromatography and DEAE cellulose anion exchanger columnchromatography. The result indicated that the enzyme had optimum pH and temperature of 4,6 and 450C, respectivelywith incubation time of 15 hours. The Km and Vmaxs values obtained from this experiment are 20 mg/ml and 0,769mg/ml/hours, respectively. Whereas the relative molecular weight of inulinase was monitored by SDS PAGE is 63KDa.

Aktivitas Promoter â-aktin Ikan Medaka Jepang (Oryzias latipes) pada Ikan Mas (Cyprinus carpio) ', Alimuddin; Purwanti, Lola Irma; Ath-thar, MH. Fariduddin; Muluk, Chairul; Carman, Odang; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This study was conducted to examine activity of medaka (Oryzias latipes) â-actin promoter (mBP) in common carp(Cyprinus carpio) as the first step towards development of common carp transgenic in country. Gene constructpmBP-hrGFP that consists of mBA promoter and humanized Renilla reniformis green fluorescent protein gene(hrGFP) was injected into cytoplasm of one cell stage of common carp by using microinjector. PmBP-hrGFPconcentration used for microinjection was 50 μg/mL aquabides. Parameters observed were survival rate of embryo(SRe), hatching rate (HR) and expression of hrGFP gene. SRe was calculated before eggs hacthed, while hatchingrate (HR) was after all of eggs hatched. The activity of mBA promoter was analyzed by observation of hrGFP genetransient expression using a fluorescence microscope. The results of experiment showed that SRe (87,5%) andHR (79.2%) of control was respectevily higher than that of injected treatment (75.0% & 61.7%). Expression of hrGFPwas observed firstly at blastula (12 hours after fertilization) to 1-day-old larval stages (24 hours after hatching)with higher gene expression at blastula to late gastrula stages. Percentage of micronjected larvae expressinghrGFP at 6 hours after hatching reached 71.6 ± 6.7%. Conclusion was that mBA promoter could drove hrGFPexpression in common carp, hence it can be used to produce common carp transgenic by changing hrGFP withgenes correlated with important traits in aquaculture.

Faktor-Faktor yang Mempengaruhi Produksi Biodiesel dari Minyak Sawit Mentah Menggunakan Katalis Padat Kalsium Karbonat yang Dipijarkan Awaluddin, Amir; ', Saryono; Nelvia, Sri; ', Wahyuni
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The demand for petroleum has increased recently due to the increase of world population, industries andtransportation. Biodiesel (fatty acids methyl esters) has become attractive because of high price of petroleum,limited recourses of crude oil, and environmental concerns. Most biodiesel is produced by transesterification oftriglycerides of refined/edible type oils using methanol and homogeneous catalyst such NaOH and KOH. The useof heterogeneous calcined CaCO3 catalyst, has advantages such as the ease of phase separation betweencatalyst and biodiesel. This paper presents factors affecting the synthesis of biodiesel from crude palm using thecalcined CaCO3 catalyst . The synthesis is carried out by two steps, the acid-catalyzed pre-esterification of free-fatty acid and followed by base-catalyzed transesterification of triglycerides. A study of optimizing the reactioncondition of the esterification followed by transesterification of crude palm oil (CPO) is performed to obtainmaximum production of biodiesel. Under conditions of catalyst calcination temperature of 9000C, reactor time of1.5 hours, catalyst dosage of 1,5%, reaction temperature of 700C and methanol/oil molar ratio of 9 : 1, the oilconversion is 74,6%. The as-synthesized biodiesel meets the requirements of Indonesian National Standard (SNI)for biodiesel.

Kowanin, Suatu Santon dari Kulit Batang Garcinia cowa Roxb ', Darwati; Bahti, Husen H; ', Supriyatna; ', Dachriyanus
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The compound tetraoxygenated xanthone was isolated from the crude extract of the stem bark of Garcinia cowaRoxb. The compound tetraoxygenated xanthone was caried out as yellow crystal with melting point 136-1370C.The structure of this compound was detremined base on spectroscopic methods, including UV, IR, 1H-NMR,13C-NMR 1D and 2D. The compound was found to exhibit cytotoxicity against T47D cell by SRB method

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Contact Name Titania T Nugroho

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Editorial Address Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

Location Kota pekanbaru, Riau INDONESIA

Abstract

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Contact Name
Titania T Nugroho

Contact Email
titania.nugroho@lecturer.unri.ac.id

Phone
+62811764031

Journal Mail Official
natur@ejournal.unri.ac.id

Editorial Address
Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

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Riau

INDONESIA