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Nonny Manampiring
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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 5 Documents
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Search results for , issue "Vol. 4 No. 1 (2023): April 2023" : 5 Documents clear
IDENTIFICATION OF THE TYPE OF FERNS IN THE FOREST AREA OF NOONGAN VILLAGE YOSUA LINU; Helen J Lawalata; Rievo Djarang
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Ferns (Pteridophyta) are spore-forming cormophytes that can live in a variety of habitats both as epiphytes, terrestrial and aquatic. In the forest area of Noongan Village, many potentials play an important role in developing forest ecosystems. One such potential is ferns. The results of the study were eight types of ferns in the forest area of Noongan village which were divided into two plots of 50x50 meters at different heights, namely plot I at an altitude of 610 masl with a total of 380 ferns with the percentage: Paku Garuda (Pteridium aquilinium) 0.20 %, Elephant fern (Angiopetris avecta Hoofm) 0.01%, Red rane fern (Selaginella australis) 0.01%, Paku Kikir (Stenosomia Sp.) 0.14%, Ferret fern (Nephrolepis biserrataSchott.) 0, 21%, cecerenean fern (Nephrolepis falcata) 0.16%, suplir fern (Adiantum.) 0.12%, andam fern (Dicranopetris linearis) 0.12%. And plot II at an altitude of 869 meters above sea level with a total of 179 ferns with a percentage of: Garuda fern (Pteridium aquilinium) 0.21%, elephant fern (Angiopetris avectaHoofm) 0.01%, miserly fern (Stenosomia Sp.) 0, 17%, Paku harupat (Nephrolepis biserrate Schott.) 0.21%, Paku cecerenean (Nephrolepis falcata) 0.15%, Suplir (Adiantum.) 0.10%, Paku andam (Dicranopetris linearis) 0.12%. In the plot I, the types and number of ferns were higher than the types and numbers of ferns in plot II. No red fern (Selaginella australis) was found in plot II. And the plant species Paku Harupat (Nephrolepis biserrata Schott). The red fern has the highest number, and the red fern (Selaginella australis.) has the least amount. The difference in altitude greatly affects the surrounding environment. So that these differences greatly affect the number of types and numbers of ferns
Beauveria bassiana FUNGUS AS A BIOLOGICAL ALTERNATIVE FOR THE CONTROL OF Bactrocera carambolae ON CHILI PLANTS Helma Fitriani; Jantje Ngangi; Helen J Lawalata
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Chili plants are an important horticultural crop for the Indonesian population. Still, in the context of pest control, the use of insecticides to manage the OPT is proving to be increasingly problematic for the environment and human health as a result of their ability to decimate microscopic organisms present in the soil so that as decomposers or decomposer microorganisms and earthworms perish, the soil would ultimately become more barren. Therefore, it needs to be overcome by finding other alternatives that are more environmentally friendly and reduce the negative impact of pesticides. This study aimed to determine an environmentally friendly alternative biological control using the Beauveria bassiana fungus. This study used an experimental method with five treatments and three replicates, notably spore concentration using B. bassiana 6g, 7g, 8g, 9g, and control. The use of this fungus is simple and does not incur significant costs. From the results of data collection, the Beauveria bassiana fungus is effective in killing carambolae with a fast mortality rate at a concentration of 9 g/500 ml of water (86.7%), and the slowest concentration is at 6 g/500 ml of water (63.4%).
Base Substitution Patterns in Partial of the Cytochrome C Oxidase Unit I (COI) mtDNA Genes in the Red Junglefowl (Gallus sp.) of North Sulawesi and some Gallus sp. accessions. Decky Kamagi
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Partial of the cytochrome C oxidase unit I (COI) mtDNA genes of the red jungle fowl (Gallus sp.) were isolated and sequenced to examine the base substitution pattern compared to several accessions taken from GenBank. Samples were collected from several areas in North Sulawesi in the form of muscle tissue and prepared with 95% alcohol, and stored at temperatures below 500 C. Total DNA was isolated using the innuPREP DNA micro kit with a modified protocol. The target gene amplification used the primers BirdR1 and BirdF1, and the amplicons were sequenced at the Singapore FirstBase laboratory. Target gene sequences have been blasted through the nucleotide blast program provided by the National Center for Biotechnology Information (NCBI)—sequence analysis using MEGA5 and DnaSPv5 software. Target gene sequences were aligned using the Clustal-W program on MEGA5. The amplified target gene sequence length was 490 bp. The blast results showed that the target gene sequence was 98% identical to the COI gene sequence from several Gallus-gallus accessions from GenBank. The results of multiple alignments between the sequences of some of the genes examined showed the presence of polymorphic sites (S). The number of polymorphic sites (S) is 11, while the conserved sequence (C) is 386/397 = 0.977. The number of haplotypes (h) is 5, and the diversity of haplotypes (Hd) is 0.703. The overall genetic distance average was 0.008. The polymorphic sites and base substitution events occurred at sites number 145 (A↔G), 241 (G↔C), 268 (C↔A), 295 (C↔A), 348 (T↔C), 381(A) ↔T), 384 (T↔C), 390 (T↔A), 393 (C↔T), 394 (T↔C) and 395 (C↔T). The substitution pattern at these polymorphic sites consists of substitution transitions and transversions. The ratio of transition substitution and transversion events (Ts/Tv) = 1.4056. The substitution events at these sites are located at the bases in the first and third positions of the codon. Substitution events at these polymorphic sites partially cause the exchange of amino acids. The exchange of amino acids occurs at codons 49, 81, 90, 99 and 130.
BIOLOGICAL CHARACTERIZATION OF INSECT PESTS Spodoptera exigua Hubner ORIGIN NORTH SULAWESI Utari Satiman; Marthy Taulu
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

One component of integrated pest control is biological control or biological control based on the bioecology of the pest by taking into account the results obtained and their long-term effects through ecology and economy. The success of controlling a type of pest requires studies on various factors that affect the life of a pest, namely biology, morphology, ecology, genetics, and evolution. Knowledge of the biology of a pest species will provide appropriate information in breaking the life cycle or making the surrounding environment not provide optimal carrying capacity so that the pest population can be suppressed below the economic threshold. Spodoptera exigua is a polyphagous insect pest that eats from many plant types. In the larval stage, this pest attacks more than 20 species of cultivated and wild plants. For over twenty years, the onion caterpillar S. exigua has been the main target for chemical control but is still the main pest on onion crops. Spodoptera exigua attacks occur throughout the year in North Sulawesi and are spread over a wide geographical range, allowing genetic differences within the species. Gene mutations cause the occurrence of genetic diversity in a species in a population. High genetic diversity in individuals in a population can be one inhibiting factor in successfully controlling a pest species. Increasing knowledge about the biology of a pest species is very important to understand its habits, reproductive ability, and distribution which will later be used to limit and control the pest population.
EFFECT OF LIQUID ORGANIC FERTILIZER FROM MARKET WASTE AND RICE WASHING WATER ON THE GROWTH OF LAND KANGKUNG (Ipomoea raptans) Arin Makasa; Orbanus Naharia
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Some of Indonesia's agricultural countries produce many types of vegetables; for example, mustard greens, cabbage, spinach, kale, and so on are usually cultivated in Indonesia. Market waste and rice washing water can make liquid organic fertilizer (POC). This research was structured to know the effect of rice washing water on land kangkung plants' growth and the concentration required in one planting period. The research location is Maesa Unima housing, Tondano District, Minahasa Regency, North Sulawesi Province. This study uses quantitative methods with the implementation of experiments. Using the RAL technique or Completely Randomized Design totaling four treatments and each repeated seven times With liquid organic fertilizer (POC) using four treatments, namely: P0: Control (Using plain water), P1: POC with a concentration of 15%, P2: POC with a concentration of 30% and P3: POC with a concentration of 45% and the parameters measured were the growth of water spinach plants, namely: land kale plant height, number of land kale plants and leaf length on land kale plants. The results showed that rice washing water affected the growth of kale plants, namely plant height, number of leaves, and leaf length, and the required concentration was 45%. The research is due to the large amount of organic and household waste in the market, which can pollute the environment if not utilized

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