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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
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Articles 402 Documents
SNP Mapping to Locate Anthracnose Resistance in Capsicum spp. Orarat Mongkolporn
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Two SNP maps were constructed from two chili populations including Capsicum annuum x C. chinense ‘PBC932’, and C. baccatum ‘PBC80’ x ‘CA1316’, aiming to identify QTLs for anthracnose resistance. The ‘PBC932’-derived map contained 12 linkage groups (LG) with 214 SNPs and 824 cM coverage. The ‘PBC80’-derived map contained 12 LGs with 403 SNPs and 1,270 cM coverage. Based on the ‘PBC932’ map, two QTLs corresponding to the anthracnose resistances in mature green and ripe fruit stages were identified on the same location of LG2 between two SNPs within 14 cM. Based on the ‘PBC80’ map, three QTLs were identified in the ripe fruit stage, which corresponded to different resistance traits that were assayed by different inoculation methods (microinjection or MI and high pressure spray or HP) with two different pathotypes (PCa2 and PCa3). All the three major QTLs for the resistance traits assayed by PCa2/MI, PCa3/MI, and PCa3/HP were located on LG4 between two SNP markers within 17 cM.
Microbial community analysis during start-up of anaerobic co-digestion based on quinone profiles using supercritical fluid extraction Asri Gani; Ahmed Fall; Hiroyuki Daimon
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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Abstract

Quinone profile is well known as a useful tool for the analysis of microbial community dynamics in mixed cultures in terms of quantification, simplicity, and reproducibility. The application of quinone profile method in anaerobic digestion is to monitor and overcome instability during fermentation process. A lab-scale anaerobic digestion treating a mixture of milk cow manure (CM) and simulated food waste (FW) during start-up process at mesophilic conditions was used to monitor the change of microbial community dynamics and stability. Supercritical fluid extraction (SFE) experiments using CO2 and ultra-high performance liquid chromatography (UPLC) was applied for extract and determination of ubiquinones (UQ) and menaquinones (MK) species. Quinone can be a helpful tool to make the link between microbial community and anaerobic digestion parameters in order to overcome digester instability during the start-up process.