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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 402 Documents
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Spontaneous initiation of parturition after two-days-course of lung maturation and controversies in management of pre-eclampsia: A case report Eva Febia; . Rajuddin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

There have been many controversies on how we should treat patients with  preeclampsia, how and when we should deliver the baby. This case report is purposed to evaluate one case of spontaneous parturition which ended up with vaginal delivery in one patient with preterm severe preeclampsia. We evaluate the best management which result in the best outcome for the patient and her baby. This is the case of Mrs N, 34 years old in 33-34 weeks gestational age with severe preeclampsia. When she came the blood pressure was 190/120 mmHg, protein urine +3. The blood pressure was controlled with nifedipine. The patient was given magnesium sulphate, N-acetyl cystein, and vitamin C as antioxidants. We were succeeded in completing two days of lung maturation with dexamethasone in order to give the best outcome for the baby. However, at the end, after two days of lung maturation, the patient started to enter the active phase of parturition spontaneously without labor of induction. We hypothesized that the high level of corticosteroid stimulated by the fetus initiated the process of labor. Patient delivered baby boy, 1300 grams, Apgar Score 7/8 that breathed spontaneously in room air. The patient’s condition was improving after parturition. Nifedipine, magnesium sulphate was considered the best management in pre-eclampsia.  Short course of lung maturation with dexamethasone was also considered to be beneficial for the baby. However, the initiation of parturition and the use of antioxidant as well as n-acetyl cystein in this patients was under debatable discussion.
Reading the pleiades star to forecast the rain: etnoastronomy of smallholder farmers in Kisar Island - Southwest Maluku Heryanus Jesajas; Brury Tumiwa
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This paper describes about indigenous knowledge and practice of smallholder farmers in Kisar island relate to rain forecasting through observing the Pleiades stars or the seven sisters stars. We reflected from a project on incorporating farmers’ indigenous knowledge into sustainable agriculture development in small islands context and four times visits in Kisar island (1999-2011). Critical question raised is to what extent the knowledge is valid and relevance. The notion of the study was a phenomenological study. A phenomenological analysis is purely description of an immediate lived experience and an expression of its meaning (Berger and Luckman, 1976; van Manen, 1990). Rationality in phenomenology is justified by an understanding of the essence of such experience rather than just causal explanation. It recognizes the important of the context and the individual construction of perception and meaning in that context. Snowball technique was applied to find the respondents or indigenousknowledge holders. Data analysis was conducted descriptively using Ethnograph v.6. The results showed that the farmers forecast the rainfall by observing the onzet and the appearance of the Pleiades stars or the seven sisters and gauging the size and the brightness of the seventh sister of the Pleiades to predictthe characteristics of the rainfall. Brighter shine and bigger appearance means more rain will come; diming and small means less rain
The effect of picloram concentrations and explants types on the induction of somatic embryo on North Aceh Cocoa genotype . Zuyasna; Siti Hafsah; Rahmi Fajri; M. Ovan Syahputra; Gilang Ramadhan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

.  The effect of picloram concentrations and three explants types on cacao somatic embryogenesis was studied. The objective of this work was to evaluate a procedure for somatic embryogenesis of cacao (Theobroma cacao L.) Aceh clones. Somatic embryogenesis was induced using floral tissue explants (petal, staminode, and anther) on North Aceh cacao genotype. No growth was observed on petal types of explants on low concentrations of picloram. Depending on the explants, the percentage of explants producing one or more embryos ranged from 3% to 75%, 5% to 80%, and 10% to 90% on media containing 3 mg/l, 4 mg/l, and 5 mg/l picloram respectively. Staminode explants produced 3 to 10 times more somatic embryos than petals, while anthers produced 2 to 5 times.  Secondary embryos were induced from the floral explants of the North Aceh cacao genotype that exhibited efficient primary embryogenesis. Percentage embryogenesis generally increased with culture time
Phylogenetics study of the black scar oyster, crassostrea iredalei in peninsular Malaysia as revealed by mitochondrial COI sequences Danial Hariz Zainal Abidin; Mohd Nor Siti Azizah; Masazurah A. Rahim; Suzana Mustaffa; Darlina Md Naim
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The present study attempts to assess the population structure and phylogenetics of the commercially important local black scar oyster, Crassostrea iredalei (Faustino 1932) from wild and cultured populations. We analyzed the mitochondrial DNA (mtDNA) sequence variation in 581 base pairs (bp) of the cytochrome c oxidase I (COI) gene among 141 individuals from eight localities within Peninsular Malaysia. The population genetics data showed a low within population nucleotide diversity, π (0.001 – 0.003) and the total haplotype diversity, h was moderate (0.417 – 0.682). Minimum spanning network revealed a complex geographical distribution of the haplotypes. Phylogenetic relationships among the populations inferred by Neighbor-Joining and Maximum Parsimony algorithm failed to detect differentiation between these sites, indicating genetic homogeneity across the populations. The findings from this study will have important implications for aquaculture, management and monitoring of cultured populations as well as conservation of wild oyster species in Malaysia.
Increment of patchouli alcohol in patchouli oil by vacuum distillation fraction method Yuliani Aisyah; Sri Haryani Anwar; Yulia Annisa
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 2 (2013): Engineering
Publisher : Syiah Kuala University

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Abstract

The quality of Indonesian patchouli oil is commonly lower than that required by standard (substandard). The quality of patchouli oil is determined by the level of an organic compound known as patchouli alcohol (PA). This research is aimed to study the influence of the initial level of patchouli alcohol in patchouli oil to the final oil quality after being separated by vacuum distillation fraction method. The patchouli oils used had three different initial level of patchouli alcohol (31.11%, 32.83% and 33.61%). The results show that the increments of the initial level of patchouli alcohol tend to lower the final concentrations of this compound in the patchouli oil. The oil with 31.11% of initial patchouli alcohol contained 49.07% PA after distillation, the initial PA of 32.83% became 42.87% and the oil with the highest initial PA (33.61%) consisted only 32.85% PA in its final quality.
An Efficient Agrobacterium-mediated Transformation Method for Sugarcane (Saccharum officinarum L.) . Efendi; M. Matsuoka
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Investigation of transformation method for sugarcane was carried out by using Agrobacterium tumefaciens with a vectors pMLH7133 that contained CaMV35S promoter and marker genes. Embryogenic callus and cell aggregates of suspension culture were used as the target materials for transformation. The aggregates cell was sonicated, and co-cultured with Agrobacterium tumefaciens. The cells were cultured in N6-2 medium containing an appropriate antibiotic to eliminate bacterial contamination. Embryogenic callus was co-cultured with Agrobacterium, and was also cultured in N6-2 medium as same as suspension culture. Selection was carried out by culturing the treated suspension culture and embryogenic callus with MS medium containing geneticin. The transformed calli were transferred to MS-R9s for shoot formation. The transformed cells were analyzed for the distribution of GUS activity histochemically. After histohemical staining with X-glux, light microscopy observation revealed that the transformed calli derived from the NiF4, Ni9, and NCo310 cultivars had blue coloration in its tissue. Thus, the gene for GUS appeared to have been transferred and to be expressed in the calli.  The construct of pCL4 was also integrated in the bacteria. Transient expression of GUS gene was successfully confirmed in the transformed Agrobacterium. When we use suspension culture, the proportion of the calli showing transient GUS expression was 4.7-fold greater with the vector in pMLH7133. We also successfully produced transformed calli with higher level of transient GUS expression. The percentage of the calli showing the best transient GUS expression is pCL4. The embryogenic callus was more competent for transfer of T-DNA into sugarcane cells. Analysis of GUS activity indicated that the gene was expressed into the calli of sugarcane. The Results indicate that the promoter can serve as an effective regulatory element to produce strong expression in callus of sugarcane. When we inoculated embryogenic callus with Agrobacterium harbored binary vector pCL4, we also successfully produced transformed calli with higher level of transient GUS expression. Thus, the gene for GUS appeared to have been transferred and to be expressed into the calli.  Putative transformed plants were tested by performing PCR and Southern Hybridization to confirm the integration and expression of the introduced genes.
Antibacterial activity of red betel (Piper crocatum) leaf methanolic extracts aginst methicillin resistant Staphylococcus aureus Tristia Rinanda; . Zulfitri; Desi Maharani Alga
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Red betel (Piper crocatum) is one of traditional medicine that has antibacterial activity. This research was conducted to determine the antibacterial activity of the methanol extract of red betle leaves against growth of Methicillin Resistant Staphylococcus aureus (MRSA). This research used Completely Randomized Design (CRD) method with 5 treatment groups consist of red betle leaves extract with concentration 150 mg/mL, 300 mg/mL, 450 mg/mL, 600 mg/mL, and negative control (sterile water) with 4 times repetition. The methanol extract of red betle leaves was obtained by maceration technique using methanol 96% as solvent. Antibacterial activity test conducted with Kirby Bauer disc diffusion method. Data of this research were analyzed by ANOVA followed by Duncan Test. The results showed that red betel leaf extracts in concentration 150 mg/mL, 300 mg/mL, 450 mg/mL, 600 mg/mL provide average inhibition zone respectively 9,0 mm, 11,2 mm, 13,6 mm and 15,7 mm. The result showed that red betel leaf extracts in tested concentration showed antibacterial activity against MRSA. The higher concentration of extract, the greater inhibition area was formed.
Adaptation of Some Clones of Sweet Potato (Ipomoea batatas L.) in the Lowlands and the Use of Manure Mardhiah Hayati; . Sabarudin; . Efendi; Ashabu Anhar; Elly Kesumawati; Nur Kamalia
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study aims to determine adaptation of some clones of sweet potato in the lowlands and the best manure application on the growth and yield of sweet potato as well as the interaction betweenthe two factors. Research conducted at the Experimental Field of the Faculty of Agriculture SyiahKuala University, Banda Aceh, with a height of 4 m above sea level, from March to September 2014. This study used a Randomized Complete Block Design in factorial arrangement (2x8), using a split plot design with three replications. The main plot is a manure treatment (control and manure 20 ton/ha), and the subplot is a treatment of types of sweet potato clones (CIP-LSQ, CIP-1945,CIP-MAN, CIP-153, CIP-CER, CIP-BDG), and local clones (beige and orange). The results showed that the best plant growth was found on CIP-153 clone, while the best crop production was CIP- LSQ clone. Growth and crop production was found best on control treatment. There are interactions between manure and clones on weight of the fresh shoot (ton/ha), fresh weight and dry bulb per plant (g), the weight of large tubers (ton/ha) and tubers yield (ton/ha). The best plant growth and production was found on a combination treatment of CIP-LSQ clones and control (without manure).
Optimization of cyclone geometry for maximum collection efficiency . Yunardi; Ilham Maulana; . Elwina; . Wusnah; Novi Sylvia; Yazid Bindar
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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Abstract

This paper presents the results obtained from the application of both computational fluid dynamics (CFD) Fluent 6.3 and Design Expert codes to modelling and optimizing a gas-solid cyclone separator based upon its geometrical parameters. A pre-processor software GAMBIT was employed to set up the configuration, discretisation, and boundary conditions of the cyclone.  A commercial CFD code FLUENT 6.3 was employed to simulate the flow field and particle dynamics in the cyclone. The optimization study was performed under either a constant gas inlet flow rate of 0.075 m3/s or a constant inlet gas velocity of 18 m/s. A response surface methodology with three levels (-1, 0, and +1) was employed as the experimental design. Independent variables to be optimized include the ratio of inlet gas width to diameter of the cyclone, W/D, the ratio of conical length to diameter, Lc/D and the ratio outlet diameter to cyclone diameter De/D. The response variables of collection efficiency and pressure drop were correlated in the forms of quadratic polynomial equations. The simultaneous optimization of the response variables has been implemented using a desirability function (DF) approach, computed with the aid of Design Expert software.  The results of investigation showed that at constant flow rate, the following optimum ratios of W/D =0,28, Lc/D =1,5,  and De/D =0,52 were obtained to give a collection efficiency of 90% and a pressure drop of 155 Pa. At the constant inlet gas velocity, the following optimum ratios of W/D =0,25, Lc/D =1,5,  and De/D =0,57 were obtained to give a collection efficiency of 90% and a pressure drop of 190 Pa. This findings indicate that gas inlet treatment at either constant flow rate or constant inlet gas velocity does not produce significant difference on the collection efficiency, but does give significant influence on the pressure drop.
Simulated data of urea of haemodialysis patient using one-compartment model Nabilah S. A. Tamrin; Noorddin Ibrahim
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

End stage kidney disease (ESKD) is a disease where both kidneys are no longer functioning properly and patients require renal replacement therapy (RRT) such as haemodialysis to prolong their life. The numbers of modality for haemodialysis had increased, as the numbers of new patient of ESKD increased. In Malaysia, the numbers of new patient had increased three-fold for the past 10 years, from year 2002 to year 2012. In this paper, demographic data, clinical data and input and output of dialysis for the selected patients are presented. The post-dialysis urea concentrations for each patient with different weightfor one compartment model were simulated using MATLAB software. The simulated values of post-dialysis urea concentration were then compared with the clinical values of post-dialysis urea concentration for different weight. The simulated values of post-dialysis urea concentration underestimated the clinical value of post-dialysis urea concentration for patient with weight less than 70kg but overestimated to patient with weight greater than 70kg. The ratio of simulated values and clinical values of post-dialysis urea concentration were found to increase with the weight of patient

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