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LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 28 Documents
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Search results for , issue "Vol 2, No 1 (2013)" : 28 Documents clear
PENGARUH PEMBERIAN FILTRAT DAUN SAMBILOTO TERHADAP JUMLAH LEUKOSIT DARAH TIKUS PUTIH YANG TERPAPAR BENZENA KHUMAIROH,; Tjandrakirana, Tjandrakirana
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Benzene is a pollutant chemical environment and leukomogen factor. One?s of the effect is decrease leukocytes concentration because benzene can attack the enzyme topoisomerasse II in the bone marrow. The composition of Andrographis paniculata are deoxyndragrapholid, andrographolid, 14- deoxy-11, neoandrogapholid, 12- didehydroandrographolide, homoandrographolide, diterpenoid, and flavonoid, and the function is immunostimulan. This research aimed to prove that Andrographis paniculata leaf filtrate can increase leukocytes concentration caused inhalation exposure benzene. This subject are 25 Wistar strain male rats were divided into five groups; normal control (K0), benzene exposure control (K1), benzene exposure+Andrographis paniculata leaf filtrate 0,15 ml (P1), benzene exposure+Andrographis paniculata leaf filtrate 0,3 ml (P2) and benzene exposure+Andrographis paniculata leaf filtrate 0,45 ml (P3). Exposure benzene for 6 hours per day on 6 days and giving Andrographis paniculata leaf filtrate for 7 days. Counting leukocytes concentration uses a haemocytometer. Analysis of the data with One-Way ANOVA and LCD at 5 % level test. The result K1 decreased to 3063 cells/mm3 (thit(2.149)>ttab(2.02)), P1  increased to 6873 cells/mm3 (thit(2.709)>ttab(2.02)); P2 7627 cells/mm3 (thit(2.420)>ttab(2.02)); P3 8803 cells/mm3 (thit(2.124)>ttab(2.02)). The analysis revealed Andrographis paniculata leaf filtrate can increase leukocytes concentration (Fhit (648.18)>Ftab (2.87), LSD (222.23). The conclution of this research that Andrographis paniculata leaf filtrate can increase leukocytes level benzene exposure.
IDENTIFIKASI SPORA JAMUR MIKORIZA VESIKULAR ARBUSKULAR (MVA) PADA TANAH TERCEMAR MINYAK BUMI DI BOJONEGORO FAIZA, RIZKA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Exploration of oil cause an oil spill or pipeline leak on a large scale, which causes contamination of land in the area. To recover petroleum contaminated land require as microbial endemic like mycorrhizal as an alternative. The objective of this research were to explore, identify and determine the density of spores of mycorrhizal of petroleum contaminated soil, besides identifying infected mycorrhizal plants. Soil samples were obtained as composite sampling during identifying and trapping of mycorrhyza. The result obtained 7 types of spore of mycorrhyza namely Glomus sp. (2 types), Gigaspora sp. (3 types), Acaulospora sp. (1 type) and Sclerocystis sp. (1 type). Plants that had symbiosis with micorrhyza were Stachytarpheta mutabilis, Lantana camara L, Imperata cylindrica L, Calotropis gigantea, Eupatorium odoratum L, Sida rhombifolia L and Tectona grandis L. The highest spore density on soil that is covered by plants Sida rhombifolia was 61 spore/50 g soil.
EFEKTIVITAS DOSIS SERBUK DAUN KENIKIR TERHADAP PENGENDALIAN NEMATODA SISTA KUNING PADA TANAMAN TOMAT RACHMAWATI, NYEMAS
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Globodera rostochiensis is one of nematode which can cause damage at tomato plant. One of the ways in controlling this pest is by using nematisida from marigolds leaf powder. This research is aimed to determine the dose which of can control Globodera rostochiensis. The research uses one direction on of RAL is the dose of marigolds leaf powder  0 g/ 2 kg ground, 25 g/ 2 kg ground, 50 g/ 2 kg ground, 75 g/2 kg ground dan 100 g/ 2 kg ground. The research uses 5 repeted treatments for 4 times so that there are 20 total treatments by using 50 cyst of Globodera rostochiensis for each treatment. Data were analyzed by using ANOVA (Analyze of varian). The results showed that the dose of marigolds leaf powder effective to Globodera rostochiensis at the dose 100 g/ 2 kg ground with controlling 62,7%.
PENGARUH KOMBINASI JENIS CENDAWAN ENTOMOPATOGEN DENGAN KERAPATAN KONIDIA TERHADAP INTENSITAS SERANGAN LARVA ULAT GRAYAK HUMAIROH, DURROH; Prayogo, Yusmani
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Armyworm is one of the important pests of soybean. This caterpillar is polifag or can attack different types of crops, vegetables, and fruits. Entomopathogenic fungus is an alternative to control armyworm development. This study aimed to determine the effectiveness of the type and density of entomopathogenic fungi conidia against the intensity of the attacks inflicted armyworm larvae. The research method used was a randomized block design (RBD) two factors. There were 12 treatments with 3 types of fungi include Metarhizium anisopliae, Beauveria bassiana and Lecanicillium lecanii conidia density using 4 types namely 106, 107, 108 and 109 conidia/ml. Each treatment was repeated 3 times. Data such as the percentage of the intensity of the attacks by the larvae at 7 days after application were analyzed using two-way analysis of variance. The results showed that the combination of the fungus Metarhizium anisopliae conidia density 108 is effective in controlling the intensity of the attacks to 34.74%
EFEKTIVITAS STEINERNEMA SP. DALAM PENGENDALIAN HAMA SERANGGA TANAH PADA BERBAGAI TEKSTUR TANAH SAFITRI, MERINA
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

The land insect pests cause huge economic disadvantages because they caused damage on agricultural plants, hence agricultural production decreases. Therefore, one of biological control was done by Steinernema sp. This research aimed to determine the effectiveness of Steinernema sp. in controlling the soil insects pest in a variety of soil textures and get the most suitable texture of soil for applications of Steinernema sp. This was experimental research using Completely Randomized Design with 1 factor treatment, The soil land textures used were sand with porosity 56.98%; argillaceous sand with porosity 57.15%; clay with porosity 60.44%; clay with porosity 50.61%; and clay with porosity 35.64% with 4 repetitions. Data obtained were analyzed with ANOVA 1 way using programs SPSS 16.0 for windows. It can be concluded that there are differences in the effectiveness of Steinernema sp. in controlling soil insect pests at various land textures. The texture of the effective application of Steinernema sp. in controlling insect pests soil is sand with porosity 56.98%.
PENGARUH FILTRAT UMBI GADUNG, DAUN SIRSAK DAN HERBA ANTING-ANTING TERHADAP MORTALITAS LARVA SPODOPTERA LITURA UTAMI NINGSIH, TRI Utami
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Spodoptera litura has polifagous quality, or a wide hoste range, which can potentially become pest in various types of food corps, vegetables, and fruits. The use of botanical pesticide environmentally can be used as one way of controlling pest S. litura. This research aimed to know the effect of filtrate Dioscorea hispida, Annona muricata, and Acalypha indica on the mortality of S. litura. This research method used one way analysis of variance (ANOVA), that is the combination of botanical pesticides filtrate. Such as the combination of the filtrate 150 grams Dioscorea hispida; 150 grams of Annona muricata; 150 grams of Acalypha indica; 75 grams of Dioscorea hispida + 75 grams of Annona muricata; 75 grams of Dioscorea hispida + 75 grams of Acalypha indica; 75 grams Annona muricata + 75 grams of Acalypha indica; 50 grams of Dioscorea hispida + 50 grams of Annona muricata + 50 gram of Acalypha indica. This research was conducted in three replications, each replication used 10 Instar larvae 2 from S. litura. The parameter which was observed was the mortality of larvae S. litura, then analyzed by ANOVA. The research result could be seen that the use of combination botanical pesticide was more influential than the giving of single botanical pesticide.
PENGARUH KOMBINASI JENIS CENDAWAN ENTOMOPATOGEN DAN FREKUENSI APLIKASI TERHADAP MORTALITAS KUTU KEBUL (BEMISIA TABACI) FITRAH J, MULYA Fitrah; Prayogo, Yusmani
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

One obstacle in current increased soybean production is impaired whitefly infestation. Economically, whitefly infestation can reduce yields by 40-80% if no control measures. Previous study showed that the Beauveria bassiana fungus was able to kill whitefly infestation, but the mortality of whitefly infestation only 50% by using a density of 108/ml conidia. This study aimed to improve mortality whitefly infestation on soybean plants using three different types of fungi and frequency of application. This study was an experimental study using a randomized block design (RBD) with two treatment factors are the type of fungus (Lecanicillium lecanii, Metarizhium anisopliae and Beauveria bassiana) and the frequency of application (1 time/week,    2 times/week, 3 times/week and 4 times/week) with three repetitions. The data obtained were analyzed by using two-way program MSTATC. The conclusion of this study were a combination of types of fungi with the frequency of applications that effectively kills fleas whitefly is a type of fungus L. lecani with frequency application 3 times/week.
CENDAWAN ENTOMOPATOGEN BEAUVERIA BASSIANA DALAM MENGENDALIKAN TELUR HAMA PENGGEREK UBI JALAR (CYLAS FORMICARIUS) ARTANTI, DITA; Prayogo, Yusmani
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

One obstacle in maintaining productivity and quality of sweet potato is Cylas formicarius pests (Coleoptera: Curculionidae). This study aimed to determine the effect of entomopathogenic fungi B. bassiana to control the eggs of C. formicarius. The method used was a Completely Randomized Design (CRD). The treatment was applied at various ages eggs that age 0 days, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, and 7 days. Each ages eggs sprayed using the fungi B. bassiana with a concentration of 108 conidia/ml with 3 repetitions. Observations were made every day for 7 days after application. Analysis of the data by one-way ANOVA and Duncan test level 5%. The results showed that B. bassiana effective in suppressing hatching eggs C. formicarius of 100% at the age of eggs 0 days to 4 days better than age of eggs 5 days to 7 days that have opportunity hatching eggs of 20-60%.
PENGARUH CARA APLIKASI DAN FREKUENSI PEMBERIAN CENDAWAN ENTOMOPATOGEN BEAUVERIA BASSIANA UNTUK MENGENDALIKAN HAMA BOLENG (CYLAS FORMICARIUS) DAN TINGKAT KERUSAKAN YANG DITIMBULKANNYA PADA UBI JALAR RIYANTI, NURIKA; Prayogo, Yusmani
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Sweet potato is one of substitute food and raw materials for some food industries. Sweet potato weevil (Cylas formicarius) is a major pest that can reduce yields and utilization of sweet potato. Utilization of entomopathogenic fungi is one of component of integrated pest management. Beauveria bassiana is a fungus that effective control of pests of the order Coleoptera. This study purposed to describe the effectiveness of the method and frequency of application of entomopathogenic fungi B. bassiana to mortality of imago C. formicarius and extent of the damage sweet potatoes. This study was an experimental research that used Randomized Block Design (RBD) arranged in factorial with two factors. The first factor was the application method on the plant and on the soil surface. The second factor was the frequency providing of 1 times, 2 times, and 3 times a week. Each treatment was repeated 4 times. The data obtained were statistically analyzed by using two-way Anava. If there is a difference then continued using multiple range test (Duncan?s Multiple Range Test) on a real level ? = 0,05. The results showed that the application method had no affect on mortality imago C. formicarius, the frequency providing of an effect on mortality imago C. formicarius at 46,88%, and the interaction of the application method and frequency providing also effects  the mortality imago C. formicarius to 70,00%. However, application method, frequency providing, and the interaction of application method and frequency method had no effect on the extent of damage to sweet potatoes.
PENGARUH 6-BENZYLAMINO PURINE (BAP) DAN 6-FURFURYL AMINO PURINE (KINETIN) PADA MEDIA MS TERHADAP PERTUMBUHAN EKSPLAN UJUNG APIKAL TANAMAN JATI SECARA IN VITRO ROSSA LINA, FARAH Rossa; Wahyono, Rahmad
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Teak is one of economic wood plants, the lack of teak supply has encouraged the expansion of teak plantation. To support the availability of plant material, propagation by tissue culture technique being a good alternative for mass production. This research was aimed at studying the responses of shoot induction and callus of top shoot of teak as result of effect of type of growth regulators, which is BAP and kinetin at MS medium through in vitro method. The study was conducted in the Laboratory Tissue Culture Nursery Permanent (KBP) Department of Agriculture and Forestry Lamongan, with BAP 1 ppm and kinetin 1 ppm on MS medium for callus growth and bud from the apical tip explants teak. Results showed that the growth response of explants apical end teak into callus and shoots. Callus growth on the third day after inoculation and shoots growth on the thirteen day after inoculation. The percentage of explant?s growth on this research is  60.46%; one that consisting of percentage of callus growth is 23.64%, the percentage growth of shoots is 12.79% and explant?s percentage that barren callus and also shoots is 24.03%.

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