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INDONESIA
Jurnal Tumbuhan Obat Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 1979897X     EISSN : 23548797     DOI : https://doi.org/10.22435/jtoi.v12i2
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology Public Health
Jurnal Tumbuhan Obat Indonesia is a journal developed to disseminate and discuss the scientific literature and other research on the development of health in Indonesian medicinal plant, includes : ethnobotany and ethnopharmacology; conservation, cultivation and post-harvest; molecular biology and biotechnology; phytochemistry; pharmacology. This journal is intended as a medium for communication among stake holders on health research such as researchers, educators, students, practitioners of Health Office, Department of Health, Public Health Service center, as well as the general public who have an interest in the matter. The journal is trying to meet the growing need to study health.
Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Ilmu Farmasi
Articles 5 Documents
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Search results for , issue "Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia" : 5 Documents clear
PROFIL METABOLIT BERBAGAI EKSTRAK DAUN Chrysophyllum cainito L. MENGGUNAKAN UPLC-QTOF-MS/MS Burhan Ma'arif; Agnis Aditama; Roihatul Muti'ah; Weka Sidha Bagawan; Reyhan Amiruddin; Rukiana Rukiana
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.88 KB) | DOI: 10.22435/jtoi.v12i1.672

Abstract

ABSTRACT Chrysophyllum cainito L. is a plant which empirically used as traditional medicine. The pharmacological effect of C. cainito is caused by secondary metabolite activity contain in the leaves. The aim of this research was to know the metabolites profile in n-hexane extract, ethyl acetate extract, and methanol extract of C. cainito leaves using UPLC-QToF-MS/MS. Dried powder of C. cainito leaves was extracted with n-hexane, ethyl acetate, and methanol with gradual extraction using Ultrasonic Assisted Extraction (UAE). Each extract was prepared with methanol and DCM solvent then injected 5 µl into UPLC-QToF-MS/MS and analyzed with Masslynx 4.1 softwares and Chemspider. The result showed that there were 28 compounds from n-hexane extract with diethyltoluamide as major compound, 47 compounds from ethyl acetate extract with loliolide as major compound, and 34 compounds from methanol extract with eplerenone as major compound. Based on literature study, there were also several compounds that likely having activity as phytoestrogens. Keyword: Metabolite profiling, chrysophyllum cainito L., UPLC QToF-MS/MS ABSTRAK Chrysophyllum cainito L. merupakan tumbuhan yang secara empiris digunakan sebagai obat tradisional. Efek farmakologi tersebut disebabkan adanya aktivitas dari berbagai senyawa metabolit sekunder yang terkandung dalam daun C. cainito. Tujuan dari penelitian ini untuk mengetahui profil metabolit ekstrak n-heksana, ekstrak etil asetat, dan ekstrak metanol daun C. cainito menggunakan UPLC-QToF-MS/MS. Serbuk kering daun C. cainito diekstraksi secara bertingkat menggunakan n-heksana, etil asetat, dan metanol dengan metode UAE. Masing-masing ekstrak dipreparasi dengan metanol dan DCM lalu diinjeksikan sebanyak 5 µl ke dalam UPLC-QToF-MS/MS, kemudian dianalisis dengan software Masslynx 4.1 dan Chemspider. Hasil menunjukkan profil metabolit dari masing-masing ekstrak daun C. cainito, yaitu ekstrak n-heksana dengan 28 senyawa dan diethyltoluamide sebagai senyawa mayor, 47 senyawa terkandung dalam ekstrak etil asetat dengan senyawa mayor loliolide, dan 34 senyawa terkandung dalam ekstrak metanol dengan senyawa mayor yaitu eplerenone. Dari studi literatur diketahui terdapat beberapa senyawa yang memiliki aktivitas sebagai fitoestrogen. Kata Kunci: Metabolite profiling, Chrysophyllum cainito L., UPLC QToF-MS/MS
KARAKTERISTIK MUTU KAPSUL RAMUAN KEBUGARAN UNTUK SAINTIFIKASI JAMU Sofa Farida; Tofan Aries Mana; Tyas Friska Dewi
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1064.133 KB) | DOI: 10.22435/jtoi.v12i1.1078

Abstract

ABSTRACT "Ramuan kebugaran" or Physical Improvement Herb is one results of Saintifikasi Jamu research that has been proven its efficacy through clinical research. The concoction herbs consists of Javanese turmeric rhizome, turmeric rhizome, meniran herbs and it was prepared as decoction. Herbs in the capsule dosage form is considered easier to consume and more appropriate in determining the dosage. This research aimed to study the right filler for capsule dosage form of “Ekstrak Ramuan Jamu Kebugaran” (ERJK) for fulfilling the quality requirements. The ERJK extraction is obtained by infundation method, the extract filtrate was added with fillers, dried, and powdered. The filler used was avicel 102 (ERJK-1), lactose (ERJK-2) and amylum (ERJK-3). The powders were tested for flowability, tapping indexes, and microorganism contamination tests. The capsules dosage form were tested for weight uniformity and disintegration time. The results of the ERJK-1, ERJK-2 and ERJK-3 quality parameters test showed the flow times were respectively 6.3, 7.8 and 5.8 seconds; the tapping indexes were 14.3, 15.35 and 13.0%; yeast count were 1.2, 2.85 and 2.95 col/g; the total plate count were 2.0; 1.0 and 1.0 col/g; capsul weight uniformity (mg) were 677.04±5.30 (CV: 0.78%); 663.95±8.54 (CV: 1.28%) and 678.38±4.93 (CV: 0.72%); capsul disintegration time (minute) were 25.60, 27.89 and 26.47. The evaluation of quality parameters shows that avicel 102 is the better filler of ERJK compared to lactose and amylum. ABSTRAK Ramuan kebugaran merupakan salah satu jamu hasil riset Saintifikasi Jamu yang efektifitas khasiatnya telah terbukti melalui uji klinik. Ramuan tersebut berupa simplisia rimpang temulawak, rimpang kunyit dan herba meniran dan disiapkan melalui proses perebusan. Ramuan dalam bentuk sediaan kapsul dipandang lebih mudah dikonsumsi dan lebih tepat dalam penentuan dosis. Tujuan penelitian ini adalah mengkaji bahan pengisi yang tepat untuk kapsul ekstrak ramuan jamu kebugaran (ERJK) yang memenuhi persyaratan kualitas. Ekstraksi ERJK dengan metode infundasi, filtrat ekstrak ditambahkan bahan pengisi, dikeringkan kemudian diserbuk. Pengisi yang digunakan adalah avicel 102 (ERJK-1), laktosa (ERJK-2) dan amilum (ERJK-3). Serbuk sampel dilakukan uji sifat alir, indeks pengetapan, serta uji cemaran mikroorganisme. Sediaan kapsul diuji keseragaman bobot dan waktu hancur. Hasil uji parameter kualitas ERJK-1, ERJK-2 dan ERJK-3 menunjukkan waktu alir berturut-turut 6,3, 7,8, dan 5,8 detik; indeks pengetapan berturut-turut 14,3, 15,4 dan 13,0%; angka Jamur berturut-turut 1,2, 2,85 dan 2,95 kol/g; angka lempeng total berturut-turut 2,0; 1,0 dan1,0 kol/g; keseragaman bobot kapsul (mg) berturut-turut 677,04±5,30 (CV: 0,78%), 663,95±8,54 (CV: 1,28%) dan 678,38±4,93 (CV: 0,72%); uji waktu hancur kapsul (menit) berturut-turut 25,60, 27,89 dan 26,47. Evaluasi parameter kualitas menunjukkan avicel 102 paling baik digunakan sebagai pengisi ERJK dibandingkan laktosa dan amilum.
ANTITHROMBOCYTOPENIA ACTIVITY OF PEANUT SHELL (Arachis hypogea L.) EXTRACT AND INFUSA ON HEPARIN INDUCED BALB/C MICE risha fillah fithria; Ririn Lispita Wulandari; Devi Nisa Hidayati; Pia Annisa; Nilam Eka Putri
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (895.35 KB) | DOI: 10.22435/jtoi.v12i1.1266

Abstract

ABSTRACT Certain group of people empirically use peanut shell stew in the treatment of thrombocytopenia due to dengue fever. The active compounds suspected to play a role in the activity of thrombocytopenia from peanut shell are flavonoids. Flavonoids can be extracted using heat and cold methods. This study aims to determine the antithrombocytopenia activity of peanut shell extract and infusa on heparin induced Balb/C mice. This study is an experimental research with randomized matched pre and postest control group design. Peanut shell extraction was done in a cool way by maseration using 70% ethanol solvent, and hot way by infundation using aquadest solvent. Forty-eight Balb/C mice were divided into 8 groups consisting of control group CMC Na 0.5 mL/20gBW/day, control group aquadest 0,5mL/20gBW/day, 3 groups of peanut shell ethanol extract (0.019, 0.038, and 0.076) g/20gBW/day, and 3 groups of peanut shell infusa (0.026, 0.052; 0.104) g/20gBW/day. All treatments were given orally. The decrease of platelet count in Balb/C mice using 26 UI/20gBW subcutan heparin induction. The measurement of the platelet count is performed by taking blood samples in the lateral veins of the tail. Platelet counts data before and after treatment was tested with dependent T-test. Differences in platelet increases across the groups were tested by 2 way anova and continued with a LSD test with 95% confidence level. The results showed that peanut shell ethanol extract had better antitrombocytopenia activity than peanut shell infusa.
UJI PENANGKAPAN RADIKAL 2,2-difenil-1-pikrilhidrazil (DPPH) OLEH EKSTRAK ETANOL BUNGA KECOMBRANG (Nicolaia speciosa (Bl.) Horan) DAN BUAH TALOK (M. calabura L. ) Tatang Irianti; Hari Purnomo; Kuswandi Kuswandi; Sindu Nuranto; Damiana Nitya Kanistri; Yosi Bayu Murti; Sofa Farida
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1314.564 KB) | DOI: 10.22435/jtoi.v12i1.1582

Abstract

ABSTRACT The process of excessive free radicals in our body plays a major part in the development of chronic and degenerative illness such as cancer, autoimmune disorders, aging, cataract, rheumatoid arthritis, cardiovascular and neurodegenerative diseases. The recently study reported that free radical and oxidants play a dual role as both toxic and beneficial compounds, since they can be either harmful or helpful to the body. Therefore, natural antioxidants can be explored to prevent degenerative diseases and in the present paper we have investigated antioxidant activity of extracts from Nicolaia speciose (Bl.) Horan flower and Muntingia calabura L. fruit for its free radical scavenging activity using 2,2-diphenyl, 1-picryl hydrazil (DPPH) radical scavenging activity. The results revealed that both ethyl acetate fractions of N. speciaose and M. calabura have the highest antioxidant activitiy with IC50 29.81 and 14.48 µg/ml respectively. The antioxidant activity of both ethanolic extracts were more potent than hexan and water fractions with IC50 39.27 and 137.20 µg/ml respectively. The investigation of IC50 values indicated that the antioxidant activity show moderate to very active. Active compounds were identified using thin layer chromatography with FeCl3, AlCl3, 2,4-DNPH and anisaldehide-H2SO4 spray reagents. Chromatogram of ethylacetate fraction showed that spots on the hRf 12 and 56 were thought to contain phenolic compounds with a carbonyl group, while the hRf 37 was suspected flavone compounds with 3-OH group and the hRf 50 was alleged the ortho-dihydroxy flavone or ortho hydroxy and free carbonyl. Chromatogram of ethyl acetate of talok fruits fraction showed that spots on the hRf 19.31 and 44 were suspected flavone compounds with ortho-hydroxy-carbonyl group and or ortho-hydroxy. ABSTRAK Radikal bebas berlebih dalam tubuh dapat memicu tumbuhnya sel kanker, penyumbatan pembuluh jantung, kerusakan oksidatif otak dan penuaan dini. Hal ini menyebabkan penelitian tentang potensi antioksidan terus mengalami peningkatan, terutama antioksidan alami dari tanaman. Bunga kecombrang (Nicolaia speciosa (BI.) Horan) dan buah talok (Muntingia Calabura L.) dilaporkan aktif sebagai antioksidan dan mengandung senyawa aktif dengan tingkat kepolaran yang berbeda. Oleh karena itu, penelitian ini bertujuan untuk menguji aktivitas dengan penangkapan radikal bebas fraksi-fraksi ekstrak bunga kecombrang dan buah talok serta karakterisasi golongan senyawa aktifnya. Hasil fraksinasi ekstrak diuji dengan larutan DPPH 0,4 mM dan diukur dengan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Hasil absorbansi sampel dan kontrol diolah untuk mendapatkan persen penangkapan radikal DPPH dan aktivitas antioksidan dievaluasi melalui nilai IC50. Senyawa aktif diidentifikasi menggunakan kromatografi lapis tipis dengan penampak bercak FeCl3, AlCl3, 2,4-DNPH dan anisaldehid-H2SO4. Fraksi-fraksi ekstrak bunga kecombrang dan buah talok menunjukkan tingkat kekuatan antioksidan antara sedang sampai sangat aktif. Fraksi etil asetat bunga kecombrang serta buah talok memiliki aktivitas penangkapan radikal bebas tertinggi dengan IC50 sebesar 29,81 µg/ml dan 14,48 µg/ml. Pada fraksi etil asetat ini, buah talok mempunyai potensi aktivitas antioksidan dua kali lipatnya dari bunga kecombrang. Fraksi air, ekstrak etanol serta fraksi heksan bunga kecombrang memiliki IC50 sebesar 39,27 µg/ml, 44,08 µg/ml, dan 135,36 µg/ml. Sedangkan ekstrak etanol, fraksi air serta fraksi heksan buah talok memiliki IC50 sebesar 137,20 µg/ml, 282,47 µg/ml, dan 2611,70 µg/ml. Hasil KLT fraksi etil asetat buah kecombrang menunjukkan bahwa bercak pada hRf 12 dan 56 mengandung senyawa fenolik dengan gugus karbonil, sedangkan hRf 37 diduga senyawa flavon dengan gugus 3-OH dan pada hRf 50 merupakan flavon dengan o-dihidroksi dan atau o-hidroksi karbonil bebas. Kemudian hasil KLT fraksi etil asetat buah talok menunjukkan bahwa bercak dengan nilai hRf 19, 31, dan 44 merupakan flavon dengan gugus o-hidroksi karbonil dan atau gugus o-dihidroksi. Bercak pada hRf 31 juga mengindikasikan adanya senyawa terpenoid.
THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS Nuning Rahmawati; Ika Yanti Marfuatush Sholikhah
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (863.621 KB) | DOI: 10.22435/jtoi.v12i1.1807

Abstract

ABSTRACT Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. ABSTRAK Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%.

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