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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 14 Documents

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Search results for , issue "Vol 45, No 03 (2013)" : 14 Documents clear

Parapagus dicephalus conjoined twins and evaluation of ischiopagus tetrapus conjoined twins in Indonesia: a case report ., ROCHADI
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Conjoined twins are rare occurrences in medical practice. In this study, two cases of conjoinedtwins, parapagus dicephalus and ischiopagus tetrapus are reported in Dr. Sardjito General Hospital,Yogyakarta, Indonesia. The first case i.e. five-day-old male parapagus dicephalus conjoinedtwins referred to the hospital. The twins have two heads, two arms and two legs. X-ray examinationreveals two vertebrae collumn, single heart, and single pelvis. Moreover ultrasonographyexamination reveals conjoining of liver and echocardiography examinatin reveals single atrium.The twins were diagnosed as parapagus dicephalus. As a result of their fusion, operative carehad been considered to be unacceptable. After several weeks in neonatal intensive care, theydied. The second case i.e. two-day-old female conjoined twins with conjoining at ischium regionreferred to the hospital. Ultrasonography and abdominal examination reveal conjoining atintestinum, buttock and ischium. The twins was diagnsed as ischiopagus tetrapus. They weresuccessfully separated at 6 months of age. They are alive and well so far for more than 7 years.In conclusion, the prognosis of conjoined twins is related to the type, extent of union and organabnormalities. The successful separation of conjoined twins might be determined by good planningand staff enthusiasm and dedication.

Cytochrome P450 aromatase (CYP19) gene expression in ovarian granulose cells of hypothyroid rats induced by propylthiouracil Totok Utoro, Prihatin Broto Sukandar Sri Kadarsih Soejono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Thyroid hormones are proven to have a direct effect on granulose cells, luteal cells and oocytesdue to their role in gonadotropin action on steroid hormone production. In vitro study showedthat tiroxine (T4) on granulose cells can stimulate ovarian steroidogenesis. Moreover, highconcentration of triiodothyronine (T3) increases the estradiol secretion and aromatase mRNAexpression. Hypothyroidism influences the cytochrome P450 aromatase (CYP19) gene expression.The aim of this study was to evaluate the expression of the CYP19 gene in granulosa cells ofhypothyroid rats induced by propylthiouracil (PTU). This was quasi experimental study withpost-test only control group design. Eleven female Sprague-Dawley rats were divided into twogroups i.e. five rats as treated group that induced by PTU 0.1 g/L in aquadest for 30 days andcontrol group that not induced by PTU. Blood sample was taken and then T4 blood level wasmeasured using an enzyme-linked immunosorbent assay (ELISA). Whereas, CYP19 gene expressionin ovarian granulose cells was measured using immunohistochemistry. Unpaired t test was usedto compare the data obtained from treated and control groups. The results showed that T4blood level on treated group (4.02 Â± 0.39 ng/dL) was significantly lower than control group(8.08 Â± 1.63 ng/dL) (p = 0.000). However, CYP19 gene expression on treated group (30.84 Â±8.01%) was not significantly different compare to control group (25.06 Â± 6.79%) (p = 0.227).In conclusion, the CYP19 gene expression in ovarian granulose cells of rats is not change afterinduction of PTU 0.1 g/L for 30 days, although the T4 blood level decreases.

The relationship between soldierâs body readiness level and the anxiousness tendency of students in the flyer academy of the Indonesian Air Force Army Adisutjipto Airbase Yogyakarta Sumarni DW, Wahyudi Cecep Sugeng Kristanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Training the Indonesian Air Force flyers takes a lot of time and fund. The cramped trainingschedule, demand of discipline and achievement, and intense stress from the fear of dying fromplane accidents give the flyer students an even bigger stress that could potentially lead toanxiousness. In the selection of flyer candidates in the field, the most important aspect to benoticed is the body readiness of the candidates, whereas the mental health is frequently leftunchecked. There was a case in a training session where a flyer student experienced a badmental and academic achievement that, after being traced, resulted from poor mental healthtest, although the military body readiness test was good. It left the country with a loss fromhaving to discharge the student. The aim of this study was to identify the relationship betweenthe military body readiness level and the anxiousness tendency of a flyer apprentice. This was anobservational study with cross-sectional design. The research subjects was the flyer students ofthe Indonesian National Air Force Army in Adisutjipto Airbase Yogyakarta. The sample was takenfrom all of the subjects which fulfilled the inclusion and exclusion criteria. The measurement ofthe anxiousness used the TMAS (Taylor Manifest Anxiety Scale), whereas the military bodyreadiness value was taken directly in the field test. Moreover, psychological stress was alsomeasured using Instrument of Psychosocial Stressor Assessment (IPSP). The results were analyzedusing Chi-Square test and multiple regression analysis. It was found that there was no significantlyrelationship between the military body readiness value and the anxiousness tendency of theflyer student in the flyer academy of the Indonesian National Air Force Army in AdisutjiptoAirbase Yogyakarta (p>0,05). The psychological stress factor has a role in creating the anxiousnesstendency of the flyer students.

Synergistic interaction between quercetin and doxorubicin on MCF-7 human breast cancer cell line Indwiani Astuti, Abdul Khairul Rizki Purba Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

The effectiveness of doxorubicin has decreased due to resistance of cancer cells. One of thenatural ingredients that are proven to reduce the resistance to anticancer is quercetin. Quercetininteracts with doxorubicin via a competition of P-glycoprotein (P-gp) transporter activity. Theaim of this study is to evaluate the interaction of quercetin and doxorubicin as cytotoxicityeffect on MCF-7 cells. Cytotoxicity test was conducted by the MTT method. Mechanism ofinteraction between doxorubicin and quercetin was evaluated with isobologram analysis.Doxorubicin and quercetin inhibited the growth of MCF-7 cells significantly. Doxorubicin andquercetin respectively had IC50 of 21ïM and 103 ïM. The interaction of doxorubicin and quercetinwere characterized by the amount of doxorubicin IC50 equivalent and quercetin IC50 equivalentless than 1 and the point-intercept of each IC50 notation equivalent plotted on the graph belowthe additive line. Analysis of isobolograms indicated that the interaction doxorubisn and quercetinin each of the ratios had synergy. Quercetin can be considered to be in a combination wit

The efficacy of ondansetron in comparison with pethidine for prevention of shivering in pregnant patients undergoing a cesarean sectionwith spinal anesthesia Bambang Suryono, Ferianto Pandit S
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Postanesthetic shivering (POS) is a common complication following spinal anesthesia. Severaldrugs have been used to prevent POS, including ondansetron and pethidine. This study wasconducted to compare the efficacy of ondansetron 8 mg with pethidine 0.4 mg/kg BW forprevention of shivering in pregnant patients undergoing a cesarean section with spinal anesthesia.This is a double blind controlled trial involving 96 pregnant patients between the age 18-40years with ASA physical status I-II, gestational age of 37-42 weeks, body weight of 40-70kg orBody Mass Index (BMI) <30, body height >145 cm who underwent a cesarean section withspinal anesthesia in Dr. Sardjito General Hospital, Yogyakarta and affiliated hospital. Exclusioncriteria included patients or families who refused to participate in the study, having a history ofallergy to bupivacaine, ondansetron, and pethidin, patients with a fetus known to have congenitalabnormalities earlier, body temperature early > 38 ÂºC or <36 ÂºC, pregnancy with complications(PEB, eclampsia, HELLP syndrome), and pregnant patients with heart disease (severe hypertension,heart trouble, abnormal heart valves). The patients were randomized into two groups i.e. 48patients of group ondansentron receiving intravenous ondansetron 8 mg and 48 patients ofgroup pethidine receiving intravenous pethidine 0.4 mg/kg BW. The patients were observed foroccurence and severity of POS, postoperative nausea and vomiting. The results showed thattwo patients (4.2%) on the ondansetron group and six patients (12.5%) on the pethidine groupexperienced of POS. Moreover, the efficacy of ondansetron in the prevention of POS (95.8%)was higher than pethidine (87.5%). However, there were not significantly different (p>0.05).The incidence of nausea on the ondansetron group (4.2%) was lower than the pethidine group(16.7%) (p<0.05). However, no significant difference in the incidences of vomiting was observedbetween the ondansetron (0%) dan the pethidine (4.2%) groups (p>0.05). In conclusion, theefficacy of ondansetron 8 mg is comparable to pethidine 0.4 mg/kgBW for prevention of shiveringin pregnant patients after spinal anesthesia.

The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Indwiani Astuti, Hajid Rahmadianto Mardihusodo Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients inIndonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.Molecular targets for anticancer drug have been identified including genes associated with cellcycle control and angiogenesis. Previously, an active and selective compound against WiDr fromTithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate theeffect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.Isolation of the active compound was performed by preparative thin layer chromatography (TLC)method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGFmonoclonal antibody. The results showed that the effect of the isolated active compound onthe WiDr cell cycle depended on the concentration and the incubation time periods. Atconcentration of 4 Î¼g/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hoursincubation and G1 phase after 72 hours incubation. While at concentration of 8 Î¼g/mL, it clearlyinhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, theisolated active compound at concentration of 4 Î¼g/mL significantly inhibited the VEGF expressionuntil 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifoliainhibited cell cycle and angiogenesis of WiDr cell.

The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Mardihusodo, Hajid Rahmadianto; Hartati Wahyuningsih, Mae Sri; Astuti, Indwiani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Parapagus dicephalus conjoined twins and evaluation of ischiopagus tetrapus conjoined twins in Indonesia: a case report . ROCHADI
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The efficacy of ondansetron in comparison with pethidine for prevention of shivering in pregnant patients undergoing a cesarean sectionwith spinal anesthesia Ferianto Bambang Suryono; . Pandit S; Bambang Suryono S
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Postanesthetic shivering (POS) is a common complication following spinal anesthesia. Severaldrugs have been used to prevent POS, including ondansetron and pethidine. This study wasconducted to compare the efficacy of ondansetron 8 mg with pethidine 0.4 mg/kg BW forprevention of shivering in pregnant patients undergoing a cesarean section with spinal anesthesia.This is a double blind controlled trial involving 96 pregnant patients between the age 18-40years with ASA physical status I-II, gestational age of 37-42 weeks, body weight of 40-70kg orBody Mass Index (BMI) <30, body height >145 cm who underwent a cesarean section withspinal anesthesia in Dr. Sardjito General Hospital, Yogyakarta and affiliated hospital. Exclusioncriteria included patients or families who refused to participate in the study, having a history ofallergy to bupivacaine, ondansetron, and pethidin, patients with a fetus known to have congenitalabnormalities earlier, body temperature early > 38 ºC or <36 ºC, pregnancy with complications(PEB, eclampsia, HELLP syndrome), and pregnant patients with heart disease (severe hypertension,heart trouble, abnormal heart valves). The patients were randomized into two groups i.e. 48patients of group ondansentron receiving intravenous ondansetron 8 mg and 48 patients ofgroup pethidine receiving intravenous pethidine 0.4 mg/kg BW. The patients were observed foroccurence and severity of POS, postoperative nausea and vomiting. The results showed thattwo patients (4.2%) on the ondansetron group and six patients (12.5%) on the pethidine groupexperienced of POS. Moreover, the efficacy of ondansetron in the prevention of POS (95.8%)was higher than pethidine (87.5%). However, there were not significantly different (p>0.05).The incidence of nausea on the ondansetron group (4.2%) was lower than the pethidine group(16.7%) (p<0.05). However, no significant difference in the incidences of vomiting was observedbetween the ondansetron (0%) dan the pethidine (4.2%) groups (p>0.05). In conclusion, theefficacy of ondansetron 8 mg is comparable to pethidine 0.4 mg/kgBW for prevention of shiveringin pregnant patients after spinal anesthesia.

Cytochrome P450 aromatase (CYP19) gene expression in ovarian granulose cells of hypothyroid rats induced by propylthiouracil Prihatin Broto Sukandar; Sri Kadarsih Soejono; Totok Utoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Thyroid hormones are proven to have a direct effect on granulose cells, luteal cells and oocytesdue to their role in gonadotropin action on steroid hormone production. In vitro study showedthat tiroxine (T4) on granulose cells can stimulate ovarian steroidogenesis. Moreover, highconcentration of triiodothyronine (T3) increases the estradiol secretion and aromatase mRNAexpression. Hypothyroidism influences the cytochrome P450 aromatase (CYP19) gene expression.The aim of this study was to evaluate the expression of the CYP19 gene in granulosa cells ofhypothyroid rats induced by propylthiouracil (PTU). This was quasi experimental study withpost-test only control group design. Eleven female Sprague-Dawley rats were divided into twogroups i.e. five rats as treated group that induced by PTU 0.1 g/L in aquadest for 30 days andcontrol group that not induced by PTU. Blood sample was taken and then T4 blood level wasmeasured using an enzyme-linked immunosorbent assay (ELISA). Whereas, CYP19 gene expressionin ovarian granulose cells was measured using immunohistochemistry. Unpaired t test was usedto compare the data obtained from treated and control groups. The results showed that T4blood level on treated group (4.02 ± 0.39 ng/dL) was significantly lower than control group(8.08 ± 1.63 ng/dL) (p = 0.000). However, CYP19 gene expression on treated group (30.84 ±8.01%) was not significantly different compare to control group (25.06 ± 6.79%) (p = 0.227).In conclusion, the CYP19 gene expression in ovarian granulose cells of rats is not change afterinduction of PTU 0.1 g/L for 30 days, although the T4 blood level decreases.

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