cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
JURNAL PEMULIAAN TANAMAN HUTAN
Published by Badan Penelitian dan Penegembangan Kehutanan
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan" : 8 Documents clear
PERTUMBUHAN STEK BATANG GMELINA ARBOREA HASIL KOLEKSI DARI LIMA POPULASI SEBARAN DI INDONESIA Hamdan Adma Adinugraha; Dedi Setiadi; Arnoldus Naibini; Nyuwito Nyuwito
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.83-91

Abstract

This study was conducted to determine the effect of plant material origin and size of cuttings on the growth ability of Gmelina. arborea stem cuttings in the nursery. The study was arranged in a randomized factorial pattern design, consisting of two factors, namely the length of the cuttings (L1 = 20 cm and L2 = 30 cm) and the cuttings size factor (small diameter class/D1 = <1.25 cm, medium/D2 = 1.25 – 2.50 cm and large/D3 => 2.50 cm). Each treatment used 5 stem cuttings and repeated in 3 times, which are grouped based on their origin population namely Bantul/P1, Bogor/P2, Lampung/P3, Bondowoso/P4 and Lombok/P5. The observationsshowed the survival percentage was 83.12% and the rooting percentage was 78.23% which was influenced by the length and diameter of the stem cuttings. The growth of shoots (number, length and diameter) and the number of shoot nodes and leaf were affected by the interaction of origin of the population and the length and diameter of the stem cuttings. Applying stem cutting method is very potential to produce planting stock of Gmelina arbore. Stem cuttings which has a biger size showed better grow of stem cuttings.
DETEKSI DAN IDENTIFIKASI Austropuccinia psidii PADA MYRTACEAE DI YOGYAKARTA INDONESIA Istiana Prihatini; I. L. G. Nutjahjaningsih; Farah Aulya Faradilla; Suranto Suranto
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.101-110

Abstract

Austropuccinia psidii is a pathogenic fungus that causes rust in the Myrtaceae plant. The extensive plantation of the host of this fungus has increased the attack of fungal pathogen, therefore, will increase the threat to the presence of Myrtaceae species around the globe including in Indonesia. This present study was aiming to detect and identify the presence of this pathogen by morphological and molecular observation. Morphological observation revealed the presence of A. psidii urediniospores on salam (Syzygium polyanthum) and kayu putih (Melaleuca cajuputi) leaves collected from the arboretum of the Indonesian Center for Forest Biotechnology and Tree Improvement (CFBTI), and the presence of teliospores on young Syzygium leaves. PCR amplification using specific primers of Ppsi1 / Ppsi6 succeeded in detecting the presence of A. psidii fungi Melaleuca and Syzygium showed by DNA amplicon length around 500bp. Efforts to obtain ITS DNA sequences to compare the molecular characteristics of fungi from two different hosts have been carried out, however, the sequencing electropherogram was unreadable, so the comparison can not be performed. This study reported that A. psidii is currently present in Myrtaceae species in Yogyakarta, therefore precaution efforts should be conducted to avoid economic and ecological impact from this pathogen.
OPTIMASI DETEKSI GEN PADA Stelechocarpus burahol (Bl.) Hook.f. & Th. MENGGUNAKAN DIRECT KIR PCR Tri Suwarni Wahyudiningsih; Dian Sartika
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.93-99

Abstract

Purification of DNA molecules from a large number of samples is laborious, costly, time-consuming, and a high risk of contamination. S. burahol leaves contain phenolic compounds, flavonoids, and terpenoids that can interfere with DNA isolation. Direct PCR kits can detect genes without DNA extraction. The objective of study was to determine the method of gene detection of Stelechocarpus burahol using the direct PCR kit. S. burahol leaf samples came from the Bogor Botanical Gardens (two trees), Garut, Purwodadi Botanical Gardens, Kyai Langgeng Gardens, Yogyakarta Palace, Turi Sleman, Wanagama, Karanganyar, and South Kalimantan. Each leaf sample of 0.1 mg was dissolved into 1.25% w / v SDS (Sodium Dodecyl sulfate) 50 ul solution, incubated at 95o C for 5 minutes, and vortexed for 2 seconds. Primers used for the trials were ITS 1F primers and 4R primers. Pre-denaturation of 95oC for 7 minutes, denaturation of 95oC for 1 minute, annealing 55oC for 1 minute, extension at 72oC for 1 minute, extension at 72oC for 1 minute, and 40 cyclus. PCR product samples of 40 - 50 μl that showed positive results were detected by electrophoresis. The results of S. burahol DNA amplification measuring ± 750 bp from ten samples of S. burahol. Direct PCR kits can be used for S. burahol gene detection, time and energy efficient, only requires a small amount of tissue, and reduces contamination due to DNA extraction. Direct PCR kits can be an effective method that can be utilized to detect target genes for large populations
PENGEMBANGAN METODE PENANDA ISOZYME PADA TREMBESI Titis Hutama Syah; Arbain Arbain
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.137-145

Abstract

Fifty samples of raintree from Sangatta, the capital city of Kutai Timur Regency, East Kalimantan were analyzed using isozyme markers to determine the characteristics of the banding pattern. The use of isozymes was intended as a biochemical marker for genetic diversity analysis. This study aimed to determine the enzyme system that could be used to determine genetic diversity of raintree. The enzyme systems used were diaphorase, esterase, and peroxidase. Polymorphism assessments were carried out on the parameters of expected heterozygosity (H), polymorphism information content (PIC), effective multiplex ratio (E), marker index (MI), discriminanting power (D), and Resolving (R). Among the three enzyme systems used, diaphorase showed consistent performance against each of the parameters assessed, with a value of H =0.475; PIC = 0.362; E =6.1; MI = 2,2; D = 0.63: and R = 4.44. However, esterase had the highest multiplex effective ratio (E = 6.16). Therefore, diaphorase is the best isozyme marker that can be used to analyze the genetic diversity of raintree.
INDUKSI KALUS EMBRIOGENIK DAN EMBRIO SOMATIK DARI EKSPLAN DAUN KULIM (Scorodacarpus borneensis Becc.) Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.73-81

Abstract

Kulim is one of woody plant that have multifunction as wood source and for spice and medicinal. Generative propagation of this plant have trouble because seed use limited. The use of leaf segment through somatic embryogenesis to solve the problem. The objective of this study is to obtain the best treatment to embryogenic callus induction. The modification of basal medium of Murashige and Skoog was used as growth medium. The experiment was conducted in three stages are callus induction, embryogenic callus and somatic embryo induction. The treatment of 2,4-D (3,0 – 12 mg/l) used for callus induction. For embriogenic callus induction used 2,4-D (3,0 – 12,0 mg/l) combined with NAA 0,5 mg/l. The treatment of thidiazuron (0,1 – 0,7 mg/l) used for somatic embryo induction. The result showed that the treatment of 2,4-D 6,0 mg/l is the best for callus induction with compact of texture, green, dry and non embryogenic. The treatment of combination 2,4-D 12.0 mg/l with NAA 0.5 mg/l is the best for friable callus induction. The treatment of 2,4-D 6.0 mg/l combined with NAA 0,5 mg/l is the best for embryogenic callus induction with very friable of texture, easy to separate, dry, smooth and glossy. Thidiazuron of 0,1 mg/l treatment is the best for somatic embryos induction with the average number of 7,8 somatic embryos.
PERTUMBUHAN AWAL KAYU MERAH (Pterocarpus indicus Willd) PADA PLOT KONSERVASI EX-SITU DI GUNUNGKIDUL UMUR 2 TAHUN Yuliah Yuliah; Ari Fiani; Tri Pamungkas
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.129-135

Abstract

Ex-situ conservation plot of kayu merah (Pterocarpus indicus Willd) has been established in Gunungkidul in2016. The purpose of this study was to observe the early growth of kayu merah in the plot at 2 years old. The plot was laid-out in randomized complete block design consisting of 68 families, 4 tree-plot and 5 blocks with spacing of 3 m × 3 m. Genetic materials of kayu merah were collected from 3 populations namely Timor, Flores and Seram. Measurement was conducted in survival rate, tree diameter, tree height and number of branches. The result showed that the average survival rate of family was high (≥80%). Based on the populations, the  survival rate of Timor, Flores and Seram were around 95%, 96% and 96.5% respectively. The average height, diameter and number of branches were 275 cm, 2.79 cm and 1.66, respectively. There were significant differences for height, diameter and number of branches among the tested families. In general, family ranking varied among the measured traits. Correlation between the traits varied among the populations. The Seram population showed the top family ranking for all the traits. It indicated that characterization in ex-situ conservation plot using genetic materials collected from the three populations was important for further conservation program of kayu merah.
PERIODE PEMBUNGAAN DAN PEMBUAHAN NYAMPLUNG (Calophyllum inophyllum) PADA TEGAKAN BENIH PROVENAN DI WONOGIRI Eritrina Windyarini; Tri Maria Hasna; Hamdan Adma Adinugraha; Budi Leksono
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.111-120

Abstract

Nyamplung (Calophyllum inophyllum) is a potential type of non-timber forest product (NTFP) with high oil yield which is useful as an alternative renewable energy sources and herbal medicine-cosmetics. Result of population selection level in Java Island have been used to build Provenan Seed Stand (PSS) nyamplung in KHDTK Wonogiri (Central Java) as an improved seed source with high oil yield and fruit productivity. Seed become nyamplung part that is used as oil raw material which is strongly influenced by flowering and fruiting period. Information on flowering fruiting ability and period is needed to support the availability of raw materials in a sustainable supply for the nyamplung oil industry. Seed become nyamplung part that is used as oil raw material which is strongly influenced by flowering and fruiting period. Information on flowering fruiting ability and period is needed to support the availability of raw materials in a sustainable supply for the nyamplung oil industry. Observation of flowering and fruiting period focused on 465 individual trees in nyamplung PSS at KHDTK Wonogiri through monthly monitoring by calculated flowering fruiting individualtree during 5 years (2014-2018). The results showed that the flowering period of nyamplung in TBP Wonogiri varied during 2014-2018. Flowering and fruiting occurs almost throughout the year. However, the peak of flowering and fruiting occurs mostly in March-August (end of the rainy season to the middle of dry season). In general, the trend of flowering and fertilization increased in 2014-2016, then decreased in 2017-2018, which is thought to be influenced by the higher canopy density. The environmental factor that most influences flowering is number of rainy days, while fertilization of TBP was strongly influenced by temperature. The management of TBP require future action such: second thinning, top pruning, application of fertilizers and hormones to stimulate flowering, and further detailed observations of flowering fruiting stage and its interaction with microclimate.
KERAGAMAN GENETIK Aquilaria malaccensis DARI BANGKA BARAT PROVINSI KEPULAUAN BANGKA BELITUNG DAN IMPLIKASINYA UNTUK PENGELOLAAN TEGAKAN BENIH I. L. G. Nurtjahjaningsih; AYPBC Widyatmoko; Liliek Haryjanto; Yuliah Yuliah; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.121-128

Abstract

Aquilaria malaccensis (agarwood) is a native species to Indonesia, it produces fragrance. Aim in this study was to assess genetic diversity and structure of A. malaccensis in order to manage seed stand to produce high quality and quantity seeds/seedlings. Leaf samples for DNA template were collected from  populations at western Bangka i.e. Air Gantang, Pelangas, and Simpang Gong. Amplifications of 14 RAPD markers were confirmed; 42 DNA samples of A. malaccensis were analysed using RAPD markers. Parameters of genetic diversity within population i.e. private alleles (PA) and expected heterozygosity (H) were calculated using GeneAlex software. Genetic structure among populations were determined as genetic distance (Da), Analysis Molecular of Variance (AMOVA), a neighbor-joining tree, were calculated using GeneAlex and PopTrew software. Eleven out of 14 RAPD markers were produced 104 stable and polymorphic alleles; PE were found at Air Gantang and Pelangas, 1 and 2 alleles, respectively. The H ranged between 0.133 (Simpang Gong) and 0.328 (Pelangas). The Da was moderate (0.099). AMOVA showed significant genetic diversity among populations (14%). A neighbor-joining tree showed the populations divided into two clusters with high boostrap value, and concordance with their geographical positions. Restricted gene flow might cause a high genetic relationship among populations. Low HEE in Simpang Gong caused this population was priored to being conserved; Air Gantang and Pelangas should be designed as different conservation units due to the population contained different private alleles.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2020 2020


Reset
Filter By Issues
All Issue Vol 16, No 1 (2022): Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan Vol 14, No 1 (2020): Jurnal Pemuliaan Tanaman Hutan Vol 13, No 2 (2019): Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan Vol 11, No 2 (2017): Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan Vol 9, No 1 (2015): Jurnal pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan Vol 8, No 1 (2014): Jurnal Pemuliaan Tanaman Hutan Vol 8, No 1 (2014): Jurnal Pemuliaan Tanaman Hutan Vol 7, No 3 (2013): Jurnal Pemuliaan Tanaman Hutan Vol 7, No 2 (2013): Jurnal Pemuliaan Tanaman Hutan Vol 7, No 1 (2013): Jurnal Pemuliaan Tanaman Hutan Vol 7, No 1 (2013): Jurnal Pemuliaan Tanaman Hutan Vol 6, No 3 (2012): Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan Vol 5, No 2 (2011): Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan Vol 4, No 3 (2010): Jurnal Pemuliaan Tanaman Hutan Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan Vol 4, No 1 (2010): Jurnal Pemuliaan Tanaman Hutan Vol 3, No 3 (2009): Jurnal Pemuliaan Tanaman Hutan Vol 3, No 2 (2009): Jurnal Pemuliaan Tanaman Hutan Vol 3, No 1 (2009): Jurnal Pemuliaan Tanaman Hutan Vol 2, No 3 (2008): Jurnal Pemuliaan Tanaman Hutan Vol 2, No 2 (2008): Jurnal Pemuliaan Tanaman Hutan Vol 2, No 1 (2008): Jurnal Pemuliaan Tanaman Hutan Vol 1, No 3 (2007): Jurnal Pemuliaan Tanaman Hutan Vol 1, No 2 (2007): Jurnal Pemuliaan Tanaman Hutan Vol 1, No 1 (2007): Jurnal Pemuliaan Tanaman Hutan More Issue