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INDONESIA
JURNAL PEMULIAAN TANAMAN HUTAN
Published by Badan Penelitian dan Penegembangan Kehutanan
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan" : 6 Documents clear
SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG ILG Nurtjahjaningsih; AYPBC Widyatmoko; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.49-56

Abstract

Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at  almost all loci except Shc-1 of S.  pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
PENGARUH CARA PENYEMAIAN DAN PEMUPUKAN NPK TERHADAP PERTUMBUHAN BIBIT MAHONI DAUN LEBAR DI PESEMAIAN Hamdan Adma Adinugraha
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.1-10

Abstract

Swietenia macrophylla King is one of popular tree species for timber product in Java. In order to increase the productivity of planting stock production at nursery, this research was conducted. The objectives of this research was to study the influences of sowing method and NPK fertilizer application on the growth of mahogany seedlings at nursery. The treatments were sowing methods (with and without pricking out), types of NPK fertilizer (grain and solution) and the dosages of NPK fertilizer (0, 0.2, 0.4 and 0.6 gram/seedlings). The result showed that sowing methods and type of NPK fertilizer did not give significant effect on the growth of mahogany seedlings. The dosages of NPK fertilizer caused significant different on the variation of height, diameter, total dry weight and seedling quality index. The application of NPK fertilizer at 0.6 g/seedlings/month showed the best result i.e: the seedling heigth (42 cm), stem diameter (5.15 mm), total dry weight (5.28 g) and seedling quality index (0.38).
VARIASI PERTUMBUHAN TINGGI PADA UJI KLON Eucalyptus pellita F. Muell. DI WONOGIRI, JAWA TENGAH Sri Sunarti
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.57-63

Abstract

Nine clones of Eucalyptus pellita F. Muell propagated from selected plus trees in the first-generation seedling seed orchard were tested together with a control of seedling in a clonal test established at Wonogiri, Central Java. The design of clonal test was Randomized Complete Block, which was laid-out as single treeplot of 16 replications with spacing of 3 m x 2 m. Measurements of tree height were conducted at age 12 months. There were significant differences between the clones in height growth. All tested clones showed better tree height than the control of seedling with superiority ranging from 42% to165%. Almost all clones also showed better performance than their original population of the first-generation seedling seed orchard. Clone repeatability for height at age 12 months was 0.90, while individual repeatability for height was 0.38. The top three clones were clones 1 and 3  which propagated from selected plus trees in first generation seedling seed orchard and clone 2 which is selected tree in border of A. mangium seed orchard.
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIFUNGAL AKAR Acacia mangium DAN AKTIVITASNYA TERHADAP Ganoderma lucidum Nur Hidayati; SM Widyastuti; Subagus Wahyuono
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.11-26

Abstract

Acacia mangium has been planted on large scale of industrial forest plantation in Indonesia, especially in Sumatera and Kalimantan islands. It has been reported that huge number of mangium plantations on those areas infected rot root disease caused by Ganoderma lucidum. To date, there was no information of mangium which resist to Ganoderma lucidum Moreover, research to get this information had been carried out with two aims as listed below: (1) isolate and identify a compound with antifungal properties from the roots of healthy mangium, and (2) identify the effect of the antifungal compound from roots of healthy mangium on Ganoderma lucidum The roots of healthy mangium from the first generation of seedling seed orchard in Wonogiri, Central Java, were used as material of this research. Mangium roots which had had their external and internal parts separated were macerated in a solvent of n-hexane and methanol. Methods of the isolation of the antifungal compound were the thinlayer chromatography (TLC), column  chromatography and thin layer preparative chromatography. Antifungal effect test was carried out by using inhibition of germination and of hyphal growth of Fusarium sp. Ultraviolet (UV) spectrometry, Infrared (IR) and Gas Chromatography – Mass Spectrometry (GC-MS) were used to identify the antifungal compound. Antifungal effect test on Ganoderma lucidum was done with a modification of the cylinder plate method, performed in vitro.The results revealed that the antifungal compound succeed isolated in its Substance B form from methanol extract from the interior of the root. Substance B showed the highest level of antifungal activity through inhibiting germination and inhibiting of germination tube growth of Fusarium sp. This was shown by the highest percentage inhibiting of germination (66,67%), and the highest percentage inhibiting of germination tube (66,03%). The inhibition zone of hyphal growth of Ganoderma lucidum macroscopically from the antifungal compound was observed at a concentration of 1800 μg/ml. Microscopically, in the area of contact with the antifungal compound, hyphal curling and distorting of tips took place at a concentration of 1500 μg/ml one day after application of the antifungal compound. Based on the analysis of GC-MS spectra, the antifungal was identified as  p-Methoxybenzylidene p aminophenol in the category of phenolic compounds.
VARIASI GENETIK UJI PROVENAN MERBAU SAMPAI UMUR 3 TAHUN DI BONDOWOSO, JAWA TIMUR Tri Pamungkas Yudohartono; Burhan Ismail
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.27-36

Abstract

Merbau (Intsia bijuga) is one of high economic value timbers. Establishment of seed source of merbau is essential tosupply qualified seeds for plantation. Center for Forest Biotechnology and Tree Improvement has established provenance test of merbau at Sumberwringin Research Forest in Bondowoso, East Java. The objectives of this study are to know survival and genetic variation of growth traits in provenance test plot of merbau. This provenance test was designed as Randomized Complete Block Design with single factor of provenance consisted of Halmahera Timur, Waigo, Oransbari, Wasior, Nabire and Seram. Each provenance comprises 3 blocks as replication and 60 treeplots of each block. The result showed high survival rate of all provenances (above 90 %). Moreover, genetic variation of height and stem diameter among provenances was also high. The best provenance for survival, and height for all observation years was Wasior. The best provenance for diameter for all observation years was Halmahera Timur. Meanwhile, provenance having the best value of survival, height and diameter for all observation years was Halmahera Timur. The  provenance having the worst value of height and diameter for all observation years was Seram. Whereas, the worst provenance of survival at 1, 2 and 3 years old were Waigo, Halmahera Timur dan Seram, respectively.
KEUNGGULAN PROVENANS ADAPTIF Eucalyptus occidentalis PADA UJI TERGENANG DAN BERGARAM Rina Laksmi Hendrati
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.37-48

Abstract

Waterloging and salinity in many places in the world are affected by natural conditions, geological history, irrigation practice and replacement of natural vegetations. Ground water might bring salt to soil surface at root zone areas. Engineering and vegetations are commonly used to anticipate this problem. Provisions of vegetations tolerate to this typical salty condition, although take a longer time to solve, are much cheaper and effective means to control salinity and even to rehabilitate the lands. E. occidentalis, a highly tolerant species to those conditions need to be tested as an alternative species for plantation under these conditions. Genetic materials from its natural distribution were tested to find out the most tolerant provenance under inundation and high salt level.Two controlled testings using 9 E. occidentalis  provenances were conducted reaching to 300 mM salt and followed by an increased level up to sea salt concentration (550mM NaCl). Provenances from the same genetic  materials were also tested in 3 field trials, i.e.: in Roberts, Sandalwindy and Kirkwood with different ranges of salt level.At salt concentration of 300mM, E. occidentalis growth was not affected, and therefore this level is too low for selection. Plants were depressed under 550mM salt and differences between provenaces are much more obvious. Different performances under high salinity were obtained from controlled condition and in field trials. Provenans Red Lake Siding and Grass Patch originated from the most extreme arid conditions nearby salty lakes showed the best ability to grow under high saline exposures on both testings.

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