cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Biomedika
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue " Vol 4, No 2 (2015): Nexus Biomedika" : 9 Documents clear
The Effect of Soy Bean (Glycine max) to mice (Mus musculus) Renal Cell’s Histophatology Induced with Reused Palm Oil prabowo, miftah nurizzahid; Listyaningsih, Endang; Afifah, Zulaika Nur
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: The reused palm oil is usually used by people to cook especially for cooking food. In the society the palm oil is usually used by deep frying method.  This methode usually destroys the chemical chain in the palm oil.As results from this deep frying methode many toxic and free radical are formed, this can be dangerous for human body. Soy bean (Glycine max) one of food that contains fitoestrogen and the descents isoflavon. The isoflavone will protect human body from free radicals and has antiinflammatory actitivity. Method: This study was an experimental laboratory using the posttest only controlled group design. The sample used 27 mice, 8-10 weeks old and weights ± 20 g. Samples were divided into 4 groups. Group of KKand KP1, in order, was only given distilled water and 0.06 ml of reused palm oil in addition to distilled waterfor 14 days. whilegroup of KP2 and KP3 was respectively given soy bean powder at a dose of 5.6 mg and 11.2 mg per 20 g of body weight each of mice alongside with 0.06 ml of reused palm oil and distilled water for 14 days. On 15th day, samples were sacrificed to be made preparations of left and right kidney by methode of parafin block and HE staining. The number of tubule proximal cell was calculated based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the Kruskal Walis test (α = 0.05) and continued with Mann-Whitney test (α = 0.05). Result: : The Kruskal Wallis test showed that there was significant difference between the four groups. Mann-Whitney test showed there was significant difference between groups of KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Soy bean (Glycine max.) can prevent the renal cell damage of mice which are induced by reused palm oil and the increase of soy bean dosage can increase protection effect to renal cell damage of mice which are induced by reused palm oil. Keywords: soy bean, reused palm oil, the renal cell damage
Hepatoprotector effect of Sesbania grandiflora L flower’s extract to Liver Cell Structure Damage of Mice caused by Over Reheated Palm Oil Fastiati, Rizka Abida; Listyaningsih, Endang; Mashuri, Yusuf
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background:Sesbania grandiflora L flower has high antioxidant activity.  This activity is caused by phytochemical ingredients of Sesbania grandiflora L flower, such as vitamin C, flavonoids  and tannin. The aims of this research were to know the hepatoprotector effect of Sesbania grandiflora L flower’s extract to the liver cell structure  damage of  mice caused by over reheated palm oil and to find out dose escalation of  Sesbania grandiflora L flower’s extract that can enhance the effect. Methods:This research was laboratory experimental study with the post-test only controlled group design. The tested animal used in this experiment were male mice, Swiss webster strain.The research population were 30 male mice divided into 5 groups, each group consisted of  6 mice. The  negative control group (KKn) was given distilled water, while the positive control group (KKp) was given over reheated palm oil. The 1st treatment group (KP1), the 2nd treatment group (KP2) and the 3rd treatment group (KP3), all, were given Over Reheated Palm Oil as much as  0.06 ml/ 20 gr BW andSesbania grandiflora L flower’s extract with dose 7 mg, 14 mg and 28 mg per 20 gr BW of mice, consecutively. The liver histological images were assessed by the sum of the cell nuclear pyknosis, karyorrhexis, and karyolysis. Data were analyzed by using One-Way ANOVA test and Post Hoc Test (LSD) (α=0.05). Results:One-Way ANOVA test showed that p value was 0.000 (p < 0.05).The results of the post hoc multiple comparisons test showed the significant differences between group KKn-KKp, KKn-KP1, KKn-KP2, KKn-KP3, KKp-KP1, KKp-KP2, KKp-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 Conclusions:Sesbania grandiflora L flower’s extract  has a protective effect on liver cell structure damage of mice (Mus musculus) caused by over reheated palm oil and the escalated dose of Sesbania grandiflora L flower’s extract  can enhance the effect. Keywords:Histological structure of liver damage, over reheated palm oil, Sesbania grandiflora L.
Correlation between Infection of Soil Transmitted Helminths (STH) and Peripheral Blood Eosinophil Levels amongElementary Student at Barengan Village, Teras Subdistrict, Boyolali. Nadhiasari, Aulia; Sakiman, Bambang Sukilarso; Dirgahayu, Paramasari
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: The Parasitic infection, caused by intestinal helminths, accounts for significans burden of human disease in developing countries including Indonesia. In chronic infection, eosinophilia occurs as protection against helminth parasites.  Increasing eosinophils is often associated with diseases, such as allergy, nephrotic syndromes, leoffler diseases, and also helminthiasis. However, there are little data to prove this unequivocally. This study aimed to determine the correlation between soil transmitted helminths infections and the levels of peripheral blood eosinophil among elementary students at Barengan Village, Teras Subdistrict, Boyolali Methods: This study was conducted by an observational analytic research in a cross sectional approach at Elementary School students in Barengan Village, Teras Subdistrict, Boyolali on periode of October-November 2014. We conducted on 74 samples according to the inclusion and exclusion criteria. The data were collected from stool, peripheral blood and questionnaires and then were statistically analyzed with Chi Square test to determine their correlation. Results: Our study showed that the prevalence of helminthiasis among student was 47.3% and Chi Square Analyzes between STH infection and eosinophilia had the value of  p=0.041 with Odds Ratio= 2.695. Conclusions:Barengan Village, Teras Subdistrict, Boyolali is still an endemic area for helminthiasis (47.3%). This study shows correlation between soil transmitted helminthes infections and increasing the levels of peripheral blood eosinophil among elementary students at Barengan Village, Teras Subdistrict, Boyolali. Keywords: Soil transmitted helminths, eosinophil, elementarystudents of SD Barengan
The Acupuncture Effect of Zusanli point (ST36) to Decrease the Number of Leukocytes in Mice (Mus musculus) Model of Sepsis Due to Exposure of Cecal Inoculum Jayanti, Esty; ., Balgis; Wulandari, Sri
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.221 KB)

Abstract

Background:The incidence of sepsis in the Dr. Moewardi Hospital at the end 2007 showed a mortality rate of 50.2 % (115 deaths from 229 patient with sepsis). Associated with the sepsis treatment therapy, the use of low-dose corticosteroids in the early stages of sepsis is still debated. WHO suggests that acupuncture can stimulate corticosteroid. The aim of this study was to know the acupuncture effect of Zusanli point ( ST36 ) to decrease the number of leukocytes in mice (Mus musculus) model of sepsis caused by the exposure of cecal inoculum. Methods:This study was an experimental laboratory research with posttest only control group designs. The samples were 28 male mice, divided by simple random sampling into a control group (KK), Sepsis Group 1 (KS1), Sepsis Group 2 (KS2), and Sepsis Group 3 (KS3). Sepsis groups were made by giving cecal inoculum exposure at a dose of 0.1 mg/mouse/day injected intraperitoneally for 7 days. During the 10 days after exposure, no treatment was given to KS1, while KS2 was treated with corticosteroid therapy, and KS3 was given acupuncture therapy. The low dose corticosteroid used was Methyl prednisolone at a dose of 0.05 mg/mice that was injected intraperitoneally 2 times daily. The acupuncture was given at the Zusanli point (ST36) with a duration of 15 minutes. Blood samples were taken at day 24th. The leukocyte count data were analyzed with One Way ANOVA test (p<0.05) and  the Bonferroni post hoc test  (p>0.005). Results:Sepsis group were successfully made by exposure of cecal inoculum with significance level (p) of 0.000 (p>0.005). Acupuncture of zusanli point (ST36) could reduce leucocyte count in mice (Mus musculus) model of sepsis caused by exposure to cecal inokulum as same as corticosteroid group with significance level (p) 0.000 (p>0.005). Conclusion:Acupuncture of  Zusanli point (ST36) can reduce leukocyte count in mice (Mus musculus) model of sepsis caused by exposure to cecal inoculum. Keywords: Acupuncture, Zusanli point (ST36), leukocyte count, Sepsis.
Study on Antiinflammatory Effect of Ethanolic Extract of Ginger Leaves (Zingiber officinale) in Albino Rats (Rattus norvegicus) Riyanti, Gisti Respati; Ediningsih, Endang; Ayusari, Amelya Augusthina
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Inflammation is a local protective response which is potentially hazard. Ginger (Zingiber officinale) contains flavonoid which has anti-inflammatory effect. Existing research has used ginger rhizomes as its material, whereas flavonoid in ginger leaves is bigger than the rhizome. This research aimed to investigate the anti-inflammatory effect of ethanolic extract of ginger leaves in albino rats (Rattus norvegicus). Methods: This research was a laboratory experimental with posttest only controlled group design. This research was conducted at Setia Budi University. The subjects were 25 albino male rats ± 3 months old, weight ± 150 gr, which were taken by purposive sampling. The subjects were divided equally into 5 groups randomly, which negative control group (KN) were given normal saline and positive control (KP) were given indomethacin whereas test group 1 (KU1), test group 2 (KU2) and test group 3 (KU3), in order, were given 20 mg, 40 mg and 80 mg of ethanolic extract of ginger leaves per 100 gr of body weight orally. Inflammation induction was done by injection of 0.05 mlcarrageenan 1% of sub plantar. Rats’ foot edema volume was observed every hour for 6 hours after injection. Statistical analysis of the data was done including one way-Anova, Kruskal-Wallis and Mann Whitney test. Results: The greatest inhibition of edema volume was at the 6th hour, KP (74.07%), KU3 (74.07%), KU2 (66.67%) and KU1 (48.15%). The Kruskal-Wallis test showed significant differences among 5 groups with p=0.002 (p<0.05). The Mann-Whitney test showed p=0.068 for KP- KU1, p=0.429 for KP- KU2 and p=0.910 for KP-KU3. Conclusions: The ethanolic extract of ginger leaves has anti-inflammatory effect in albino rats equivalent to indomethacin on the sixth hour after carrageenan induction. Keywords: ginger leaves, Zingiber officinale, anti-inflammatory, carrageenan, albino rats  
Interleukin-10 of Positive and Negative HIV RNA Patients in Dr. Moewardi General Hospital in Surakarta Pradeta, Emirita Wulan; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: HIV infection may cause imbalance of immune system and impacts on IL-10 status which has several immunological roles that may affect HIV infection itself. The aim of this study was to investigate the association between IL-10 and HIV RNA status. Methods: The IL-10 levels in the plasma samples obtained from 60 HIV patients in Dr. Moewardi General Hospital in Surakarta were analyzed by ELISA (Enzyme-Linked Immunosorbent Assay). Results: Elevated IL-10 level was found in 21.7% (13/60) samples and predominantly in positive HIV RNA (OR 2.67; 95% CI 0.40-17.96) and male (OR 1.22; 95% CI 0.36-4.17) patients. Conclusion: Elevated IL-10 level was associated with positive HIV RNA status and gender. Keywords: IL-10, HIV RNA
Toxoplasma gondii Coinfection in HIV/AIDS Patients of Dr. Moewardi Hospital in Surakarta Raharjo, Irvan; Sari, Yulia; Prasetyo, Afiono Agung
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Coinfection of T. gondii and HIV/AIDS will result in severe clinical symptoms. Age and gender are risk factors that are often associated with the occurrence of this coinfection. This study aimed to determine the seroprevalence of T. gondii and HIV/AIDS coinfection as well as to determine its association with age and gender. Methods: Serological test, ELISA, was performed in order to determine the presence of IgM anti-Toxo and IgG anti-Toxo in 51 blood samples taken from HIV/AIDS patients at Dr. Moewardi Hospital in November 2011. Results: The positive rates of IgM anti-Toxo and IgG anti-Toxo were 25.5% (13/51) and 17.6% (9/51), respectively. IgM anti-Toxo was associated with age ≥ 37 years (aOR 9.34 (95% CI 1.775–49.174; p = 0.008)). Unless otherwise stated, no statistical association could be drawn. Conclusions: Screening on T. gondii infection is recommended for all HIV/AIDS patients since the positivity rates of anti-Toxo antibodies were found high in these patients. Keywords: Toxoplasma gondii, HIV/AIDS coinfection 
Soil-Transmitted Helminths (STH) Infection has no Relations with Anemia on Childrenin SDN Barengan, sub-district Teras, Boyolali Salsabila, Aryanda Widya Tazkagani; Dirgahayu, Paramasari; Randita, Amandha Boy Timor
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: STH infections with prevalence degree of 28% become a problem in Indonesia societies, especially those who are trapped in low socio-economic level. The infection often affects children because children commonly lack of knowladge about personal sanitation, and their innate immune response has not well developed yet. STH infection can cause deficiencies of calories and protein, it also can cause anemia. An area with high humidity level like sub-district Teras in Boyolali is such a comfortable place for STH to grow rapidly. This study aimed to investigate whether there were any relation between STH infection and anemia on children in SDN Barengan, sub-district Teras, Boyolali. Method: This was an Observational analityc research with cross sectional approach. This research took place in SDN Barengan, sub-district Teras, Boyolali. Sampling technique for this research was undertaken with technique of purposive sampling. It took qualified students that met criterias, from first grade until fifth grade. The data were taken from feces examination by formol ether concentration method, hemoglobin examination by cyanmethemoglobin method, parents’ socio-economic demographic questionnaire, personal sanitation questionnaire, and foodrecall. The research was obtained as many as 74 research subjects, and analyzed by chi square. Result: The data showed 35 children (47.3%) were positively infected by STH disease and 11 children (14.9%) positively suffer anemia. The analysis of data showed that there was no significant relations between STH infection and anemia, p = 0.431. Analysis of most dominant factor that affects STH infection was bad habbit of barefooted with p = 0.000 (p < 0.05). Meanwhile, anemia disease was mostly caused by lack of protein intake with p = 0.048 (p < 0.05). Conclusion: STH infection has no significant relations for increasing the prevalence of anemia on children in SDN Barengan, sub-district Teras, Boyolali. Keywords: soil-transmitted helminth infection, anemia, primary school children
The Difference of FEV1/FVC Ratio Between Junior High School Students Living in Highland and Lowland Fauzi, Fajar Shodiq Irsyad; Jusup, Sinu Andhi; ., Martini
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Geographically, highland is a place that has an altitude more than 500 meters above sea level. Highland has some environmental characteristic differences, thus people need an adaptation process that affects their multiple organ system. One of the systems affected is respiration system. FVC (forced vital capacity) is the total volume of air that can be forcibly exhaled in one breath. FEV1 (forced expiratory volume 1st) is the volume of air that can be exhaled in the first forced expiration. FEV1/FVC ratio is one of the lung function parameters that can describe condition of the airway. This research aimed to analyze the difference of FEV1/FVC ratio between junior high school students living in highland and lowland. Method: This research was a cross-sectional observational analytic. Samples of this research were 9th grade students of Junior High School (SMP) Amal Mulya Tawangmangu, Karanganyar and Junior High School (SMP) Negeri 2 Kretek, Bantul.  The data were collected by using non-random purposive quota sampling with inclusion and exclusion criteria that had been specified. FEV1/FVC ratio data were measured by Minato Autospiro AS300 Spirometer, while the Respiratory rate and the chest circumference index were collected as supporting data. The data were analysis with the Kolmogorov-Smirnov test, the Mann-Whitney test and the independent t-test. Result: The Kolmogorov-Smirnov test showed that the FEV1/FVC ratio were not normally distributed. Mean of FEV1/FVC ratio of junior high school students living in highland was 98.52±2.15 whereas mean of its living in lowland was 97.65±3.30. The result of the Mann-Whitney comparative test showed p = 0.959 (p>0.05). Conclusion: There is no significant difference of FEV1/FVC ratio between junior high school students living in highland and lowland. Keywords: FEV1/FVC ratio, highland, lowland, lung function, spirometer

Page 1 of 1 | Total Record : 9

 1 

Filter by Year

2015 2015


Reset
Filter By Issues
All Issue Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika More Issue