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Pengaruh Ekstrak Daun Lamtoro (Leucaena leucocephala) terhadap Waktu Kematian Cacing Ascaris suum, Goeze In Vitro Ramadhan, Mira Rizki; Dharmawan, Ruben; Mashuri, Yusuf Ari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background. Nematodes infection particularly, Ascaris lumbricoides  is cronic infection that make serious health problems. Leucaena leaf (Leucaena lecocephala) contain  leucanin, leucanon, mimosin and tannin that have been known to have athelmintic effect. This study aimed to determine the effect of Leucaena. Leucaena leaf  (Leucocaena leucocephala) extract towards death time of Ascaris suum, Goeze In Vitro.. Methods. This study was a laboratory experimental research using the post-test only controlled group design. The subject were adult worms that have length between 10 -20 cm. The sampling technique was used in purposive sampling. The subject was divides into 6 groups, each group consist of 4 worms, replication performed 4 times. Pirantel Pamoat 5mg/ml was used in Positive Control Group (KKP) and NaCl 0,9% was used in Negative Control Group (KKN). While the handling group was given by leucaena leaf extract in various concentration. 5% b/v was given by first handling group (KP1), 10% b/v was given for KP2, 20% b/v was given for KP3, and  30% b/v was given for KP4. Worms immersed every 60 minutes and counted the number of time. Data were analyzed with regression liner and probit analys. Results. The fastest of death time that given by leucaena leaf is KP4 in the amount of 375 minutest, followed by KP3, KP2 dan KP1. While the death time of  KKP aproximately  at 60 minutes dan 3360 minutes on KKN. The result of Pearson correlation test show close relationship between variation of the concentration and the time of death worms with a correlation coefficient 0,778 marked negative. The result of regression liner show significant p < 0.000,  r square .605 with regression equation is Y = 2704 – 503,464 X. The result of probit analys LC50 in the amount of  10,355%, LT50 is counted at 411,178 minutes LT99 is counted at 571,621 minutes.  While  37,055 minutes is counted for Pirantel Pamoat. Conclution. Based on this study, in can be concluded that the  Leucaena leucocephala leaf extract affect mortality of Ascaris suum in Vitro. The increasing of extract concentration in inversely propotional with the death time of worms. Keywords : Leucaena Leaf extract, death time,  anthelmintic 
The Association between Degree of Smoking and Myocardial Infarction Prevalence Subijanto, Achmad Arman; Mashuri, Yusuf Ari; Nardian, Derajat Fauzan
Nexus Kedokteran Komunitas Vol 5, No 2 (2016): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Cardiovascular disease is one of the leading causes of death in the world. One of the most common cardiovascular disease is myocardial infarction. In developing countries such as Indonesia, smoking is a risk factor that greatly affects on myocardial infarction disease. Many factors such as the duration of smoking, the number of cigarette consumption and the degree of smoking are thought to be related to the prevalence of myocardial infarction. The aim of this research was to determine the association between degree of smoking and myocardial infarction prevalence. Methods: The study is analytical observation by using cross sectional design. The subjects were patients who came to cardiovascular clinic and inpatients in cardiovascular unit of Dr. Moewardi Hospital Surakarta. The subjects were selected by purposive sampling method. The independent variable is degree of smoking that was obtained by interview and questionnaire and the dependent variable is the myocardial infarction status that was obtained by medical record of the subjects. Data were analyzed using chi-square test with p value = 0.05. Results: The total number of patients with history of myocardial infarction were 22 (50%) whereas patients without history of myocardial infarction were 22 (50%). The number of subjects with light smoking status were 18 (40.9%), moderate smoking status were 15 (34.1%) and heavy smoking status were 11 (25%). Chi-square test on the degree of smoking and the prevalence of myocardial infarction showed that the odds ratio between heavy smoking status and light smoking status was 26 and odds ratio between heavy smoking status and moderate smoking status was 11.43 whereas p value was 0.004 (p < 0.05). Conclusion: It can be concluded that there is difference in myocardial infarction prevalence between heavy, moderate and light smoking status patients in Dr. Moewardi Hospital Surakarta. Keywords: cigarrete, smoking status, degree of smoking, myocardial infarction 
Association Between Stress at Work and Onset of Menopause in Senior High Schools Women Teachers in Surakarta Arditya, Canda; Riyadi, Slamet; Mashuri, Yusuf Ari
Nexus Kedokteran Komunitas Vol 6, No 2 (2017): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Bacground: Stress has been already known affecting hormonal axis. However, the association between stress and menopause is poorly understood. The study was aimed to determine the association between stress at work and the onset of menopause of the women teachers in Senior High Schools in Surakarta. Methods: This was an observational cross-sectional research using cross sectional design. The sample was active women teachers who were regularly  teaching in Senior High Schools at Sura’karta who already had menstrual periods stop. Sampling was done using a simple random sampling method with amount of sample 58 women teachers. The data of stress at work were obtained by International Stress Management Assosiation (ISMA) quistionare and the onset of menopause were obtained by interviews. The data were analyzed with unpaired t-test and Spearman correlation test. Results: Stress at work  was significantly correlated with the onset of menopause (r = -0.441; p = 0.009). Stress at work was found to be significantly associated with onset of menopause  (p < 0.05). Conclusion: This research shows a significant correlation between work stress and the onset of menopause with negative direction and  moderate strength. Keywords: menopause, stress at work
Hepatoprotector effect of Sesbania grandiflora L flower’s extract to Liver Cell Structure Damage of Mice caused by Over Reheated Palm Oil Fastiati, Rizka Abida; Listyaningsih, Endang; Mashuri, Yusuf
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background:Sesbania grandiflora L flower has high antioxidant activity.  This activity is caused by phytochemical ingredients of Sesbania grandiflora L flower, such as vitamin C, flavonoids  and tannin. The aims of this research were to know the hepatoprotector effect of Sesbania grandiflora L flower’s extract to the liver cell structure  damage of  mice caused by over reheated palm oil and to find out dose escalation of  Sesbania grandiflora L flower’s extract that can enhance the effect. Methods:This research was laboratory experimental study with the post-test only controlled group design. The tested animal used in this experiment were male mice, Swiss webster strain.The research population were 30 male mice divided into 5 groups, each group consisted of  6 mice. The  negative control group (KKn) was given distilled water, while the positive control group (KKp) was given over reheated palm oil. The 1st treatment group (KP1), the 2nd treatment group (KP2) and the 3rd treatment group (KP3), all, were given Over Reheated Palm Oil as much as  0.06 ml/ 20 gr BW andSesbania grandiflora L flower’s extract with dose 7 mg, 14 mg and 28 mg per 20 gr BW of mice, consecutively. The liver histological images were assessed by the sum of the cell nuclear pyknosis, karyorrhexis, and karyolysis. Data were analyzed by using One-Way ANOVA test and Post Hoc Test (LSD) (α=0.05). Results:One-Way ANOVA test showed that p value was 0.000 (p < 0.05).The results of the post hoc multiple comparisons test showed the significant differences between group KKn-KKp, KKn-KP1, KKn-KP2, KKn-KP3, KKp-KP1, KKp-KP2, KKp-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 Conclusions:Sesbania grandiflora L flower’s extract  has a protective effect on liver cell structure damage of mice (Mus musculus) caused by over reheated palm oil and the escalated dose of Sesbania grandiflora L flower’s extract  can enhance the effect. Keywords:Histological structure of liver damage, over reheated palm oil, Sesbania grandiflora L.
The Effect of Ethanol Extract of Cat’s Nettle Leaf (Acalypha indica Linn) to The Mortality of Aedes aegypti Larvae Mashuri, Yusuf Ari; Rizki, Yunika Varestri Anugrah; Sakiman, FX. Bambang Sukilarso
Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Introduction: The incidency of Dengue Hemorrhagic Fever (DHF) in Indonesia is high. The best way of prevention is cutting the infection chains, one of them is using larvasida. Methanol extract of cat’s nettle leaf is known as the killer of Aedes aegypti larvae in India. Here, the study is to examine if ethanol extract of cat’s nettle leaf in Indonesia has same larvasida affect toward Aedes aegypti. It is because the cat’s nettle leaf in Indonesia may have different variations. Methods: The study was a laboratoric experimental which was performed in Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga, Central Java. The subject was cat’s nettle leaf which was obtained from Sleman, Yogyakarta. Also, the indicator was 750 third instar Aedes aegypti larvas which were obtained from B2P2VRP Salatiga, Central Java. Ethanol extract of cat’s nettle leaf was obtained from Pharmacology Laboratory of Gadjah Mada University. The study was a post test only control group design with 1 control group and 5 treatment groups. Each group was consisted of 25 larvae and was examined four times. Aquades of 100 ml was administered to the control group. While, the ethanol extract of cat’s nettle leaf with concentration of 0,03%, 0,06%, 0,09%, 1,2% and 1,5% were administered to the treatment group. After 24 hours, observation and calculation of total larvae death were established. The data was analyzed by using Probit Regression and Curve Estimation. Results: The percentage of dead larvae in 0,03%, 0,06%, 0,09%, 1,2% and 1,5% of cat’s nettle leaf’s ethanol extract were 58%, 63%, 71%, 91% dan 94% respectively. The statistic result of Probit Regression shows that LC50 and LC99 are on 0,029% and 0,61% concentration respectively. Also, the Curve Estimation result shows that there is effect of ethanol extract of cat’s nettle leaf to the mortality of Aedes aegypti larvae with tight correlation R² = 0,888 and the linear regression equation is Y = 0,478 + 3,073 X. Conclusion: The ethanol extract of cat’s nettle leaf induced effect to the mortality of Aedes aegypti larvae with LC50 on 0,029% concentration and LC99 on 0,61% concentration. Keywords: Aedes aegypti  larvae,  etanol extract of cat’s nettle leaf,  LC50 and LC99
Effectivity of Ethanol Extract of Bintaro Seed (Cerbera manghas) as Larvacide against Anopheles aconitus Larvae Natsir, Muhammad; Sakiman, Bambang Sukilarso; Mashuri, Yusuf Ari
Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Introduction: Malaria is still one of the public health problem that can cause death, especially in the high risk groups; babies, toddlers, and pregnant women. One of the strategy to reduce the number of cases is vector management and control using larvacides which are derived from plants. Bintaro seed (Cerbera manghas) is known containing cerberins, saponins, tanins, and steroids. This study aimed to determine effectivity bintaro seed (Cerbera manghas) ethanol extract as a larvacide against Anopheles aconitus larvae. Methods: This was a laboratory experimental research with post test only control group design. The object of this research was the larva of Anopheles aconitus that was divided into seven groups with each group contains 25 larvae. The  research was replicated four times. The negative control group was 100 ml of aquadest. The other six groups contained different concentration of ethanol extract of bintaro seed. They were 0,125%, 0,25%, 0,375%, 0,5%, 0,625%, and 0,75%. The samples then were observered after being stored in 24 hours and the number of dead larvae were counted. The data were analyzed using Probit Regression and Curve Estimation. Results: LC50 and LC99 were 0,118% and 0,728% respectively. The Curve Estimation showed that ethanol extract of bintaro seed affects the mortality of Anopheles aconitus larvae with tight correlation R2 = 0,709. Its linear regression equation was Y = 0,592 + 0,641 X. Conclusions: The ethanol extract of bintaro seed (Cerbera manghas) is effective as a larvacide against Anopheles aconitus larvae with its LC50 in 0,118% and LC99 0,728%. Keywords: bintaro seed (Cerbera manghas), Anopheles aconitus larvae
Risk Factors and Prognosis of Nontuberculous Mycobacteria Infection in a High Prevalence of Tuberculosis Setting Saptawati, Leli; Mashuri, Yusuf Ari; Suryawati, Betty; Harsono, Harsono; Pradiptakirana, Riska
Journal of Epidemiology and Public Health Vol 5, No 1 (2020)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: The prevalence and incidence of Non­tuberculous Mycobacteria (NTM) infections in the world in general have continued to in­cre­ase in the last two to three decades. Until now i­den­tification and testing of NTM sensitivity in Indonesia is still very difficult to do in daily health services. Thus, NTM infection is still a ne­glected case and misdiagnosis often occurs. In these conditions, one of the things that is very im­portant to help clinicians in the management of NTM infections is information about patient characteristics and how the relationship of risk factors for NTM infection with prognosis.Subjects and Method: The study was con­duc­ted retrospectively using data on the medical re­cords of patients with positive culture of NTM at the Surakarta Center for Community Lung Health (BBKPM) for 3 years (2016-2018). A total of 134 samples were selected for this study with total sampling. The dependent variable is the prognosis of infection. The independent variables were gender, age, occupation, history of inhaled corticosteroid use, antibiotic therapy, and under­lying disease. Data were analyzed using multiple logistic regression.Results: The number of samples is 143 medical records. Most of the patients in this study were male (62.90%) and aged less than 60 years (79.70%). The majority of patients work not as far­mers (80.40%). Most patients had no history of inhaled corticosteroid use (89.50%). The­ra­pe­utic factors indicate that the majority of patients receive combined antibiotic therapy (68.5%). As many as 42% of patients with NTM infection have an underlying disease. Underlying disease in patients is mostly in the form of diabetes me­l­litus (DM) in 13.3% and in the former tuber­cu­losis in 4.9%. Based on the Chi-Square test, a sig­nificant relationship was found between the­ra­peutic factors, history of inhaled corti­cos­teroid use, and type of work, and the patient's prog­no­sis. Based on mult­ivariable testing, the factor of antibiotic therapy is the most influential variable on a pa­tient's prognosis.Conclusion: Risk factors related to the patient's prog­nosis are occupation, use of inhaled cor­tico­steroids and antibiotic therapy.Keywords: Nontuberculous Mycobacteria, risk factors, prognosis, Center for Community Lung HealthCorrespondence: Leli Saptawati. Department of Microbiology, Fa­culty of Medicine, Universitas Sebelas Maret, In­do­nesia. Jl. Ir. Soetami no. 36A Kentingan Su­ra­karta, Indonesia. Email: llsapt_md@yahoo.­co.­id. Mobile: (0271) 632489.Journal of Epidemiology and Public Health (2020), 5(1): 79-87https://doi.org/10.26911/jepublichealth.2020.05.01.08
 The Effect of Short Stature on Children’s Cognitive Abilities and Psychosocial Condition Hastuti, Heni; Nisa, Zahra Dzakiyatin; Umma, Husnia Auliyatul; Putri, Anak Agung Alit Kirti Estuti Narendra; Mashuri, Yusuf Ari
Journal of Maternal and Child Health Vol 5, No 3 (2020)
Publisher : Journal of Maternal and Child Health

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Abstract

Background: Children are categorized as short stature when the Height for Age results are below the 3rd percentile of CDC growth chart standard. Short stature on children will have an impact on increased mortality and morbidity, children’s cognitive development, and psychological abilities. This study aims to investigate the effect of short stature on cognitive abilities and psychosocial condition of primary school children.Subjects and Methods: This was an observational analytic research with a cross-sectional design. A total of 81 students from 3rd – 5th grades of Pasar Kliwon 01 Primary School was included in this study. A consecutive sampling technique was used to select the subject. The student’s height was measured by using microtoise. Children’s cognitive ability was assessed based on mathematics, Indonesian languages, and natural sciences score. Children’s psychosocial condition was assessed using Pediatric Symptom Checklist-17. Data were analyzed using the Chi-Square test and logistic regression with SPSS 16.0 for windows.Results: 50 of 81 students were classified as normal height and 31 students with short stature. Short stature children had lower cognitive abilities (p=0,047) and psychosocial condition (p=0,027) than normal height children. Sex (p=0,058), age (p=0,233), and socio-economic condition (p=0,767) were not associated with children’s cognitive abilities. Age (p=0,264), sex (p=0,913), and socio-economic condition (p=0,138) were also not associated with children’s psychosocial condition.Conclusion: There is a statistically significant effect of short stature on children’s cognitive abilities and psychosocial condition in primary school. Age, sex, and socio-economic condition are not associated with children’s cognitive abilities and psychosocial condition. Screening and comprehensive management of short stature are needed to minimize the negative effect.Keywords: short stature; cognitive ability; psychosocial condition.Correspondence: Heni Hastuti. Faculty of Medicine, Universitas Sebelas Maret. Jl Ir. Sutami 36A, Surakarta Central Java, Indonesia. Email: henihastuti.1988@gmail.com. Mobile: 081904534310.Journal of Maternal and Child Health (2020), 05(03): 297-303https://doi.org/10.26911/thejmch.2020.05.03.08
The Relationship Between Soil-transmitted Helminth Infections and Clean and Healthy Living Behavior Zuchaliya, Anifa Chofsoh; Sari, Yulia; Setyawan, Sigit; Mashuri, Yusuf Ari
Disease Prevention and Public Health Journal Vol 15, No 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.3159

Abstract

Background: Soil-transmitted Helminth (STH) are nematodes that can infect humans through direct contact with soil due to poor hygiene behavior. School-age children are the most susceptible to the infection. The clinical symptoms of STH infection are usually asymptomatic. However, it can affect the quality of life of the infected. This study aims to analyze the relationship between STH infection with clean and healthy living behavior; Method: This study takes the form of analytic observational research with a cross-sectional approach. The sampling technique used was the total sampling method with subjects of 132 students of Sekolah Dasar (SD) 1 Talakbroto, SD 1 Kedunglengkong, and SD Wates, Simo, Boyolali. The data were obtained from the results of the students' clean and healthy living behavior questionnaire and the examination of stool samples using the Kato Katz technique. Data were presented in a categorical form and analyzed using the chi-square method.; Results: There were 15 students (11%) infected by STH from 132 subjects studied. Based on the chi-square test, the p-value of STH infection and clean and healthy living behavior was 0.043 (p<0,05); Conclusion: There is a significant relationship between STH infection with clean and healthy living behavior. 
The Relationship Between Soil-transmitted Helminth Infections and Clean and Healthy Living Behavior Anifa Chofsoh Zuchaliya; Yulia Sari; Sigit Setyawan; Yusuf Ari Mashuri
Disease Prevention and Public Health Journal Vol. 15 No. 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.3159

Abstract

Background: Soil-transmitted Helminth (STH) are nematodes that can infect humans through direct contact with soil due to poor hygiene behavior. School-age children are the most susceptible to the infection. The clinical symptoms of STH infection are usually asymptomatic. However, it can affect the quality of life of the infected. This study aims to analyze the relationship between STH infection with clean and healthy living behavior; Method: This study takes the form of analytic observational research with a cross-sectional approach. The sampling technique used was the total sampling method with subjects of 132 students of Sekolah Dasar (SD) 1 Talakbroto, SD 1 Kedunglengkong, and SD Wates, Simo, Boyolali. The data were obtained from the results of the students' clean and healthy living behavior questionnaire and the examination of stool samples using the Kato Katz technique. Data were presented in a categorical form and analyzed using the chi-square method.; Results: There were 15 students (11%) infected by STH from 132 subjects studied. Based on the chi-square test, the p-value of STH infection and clean and healthy living behavior was 0.043 (p<0,05); Conclusion: There is a significant relationship between STH infection with clean and healthy living behavior. 
Co-Authors Achmad Arman Subijanto Amalia Aryasita Dewi Amelya Augusthina Ayusari Amira Masiah Syahvira Anifa Chofsoh Zuchaliya Arditya, Canda Ari Natalia Probandari Astrida Fesky Febrianty Balgis Balgis Bambang Sukilarso Sakiman Canda Arditya Derajat Fauzan Nardian Dian Ariningrum Dian Ariningrum Dono Indarto Dwi Rahayu Endang Listyaningsih Eti Poncorini Pamungkasari Evi Nurhayatun Fajar Taufik Nurseha Fastiati, Rizka Abida Felizia Alika Yusman FX. Bambang Sukilarso Sakiman Giani, Maia Thalia Grefima Pramudani Harsono Harsono Hastuti, Heni Heni Hastuti Husnia Auliyatul Umma, Husnia Auliyatul Ipop Sjarifah Ipop Sjarifah Kenneth Tan Leli Saptawati Lilik Wijayanti Listyaningsih, Endang Lukman Aryoseto Maia Thalia Giani Maria Galuh Kamenyangan Sari Mira Rizki Ramadhan Mira Rizki Ramadhan, Mira Rizki Muchtar Hanafi Muhamad Ramdani Ibnu Taufik Muhammad Natsir Nardian, Derajat Fauzan Natsir, Muhammad Naufal Irsaly Zikri Nisa, Zahra Dzakiyatin Novelia Qothrunnada Nur Adhi Hutomo Nurhayatun, Evi Pitut Kristianta Nugraha Pradiptakirana, Riska Primaningtyas, Widana Putri, Anak Agung Alit Kirti Estuti Narendra Rahmania Chandra Savitri Ratih Puspita Febrinasari Ratih Puspita Febrinasari Ratih Tri Kusuma Dewi Ratih Tri Kusuma Dewi, Ratih Tri Kusuma Richard Philo Riyadi, Slamet Rizka Abida Fastiati Rizki, Yunika Varestri Anugrah RUBEN DHARMAWAN Ruben Dharmawan Sakiman, Bambang Sukilarso Sakiman, FX. Bambang Sukilarso Shofura Azizah Sigit Setyawan Sigit Setyawan Slamet Riyadi Sonya Dellania Raharja Sri Haryati Subijanto, Achmad Arman SURYAWATI, BETTY Sutartinah Sri Handayani Sya&#039;irul Tandi Alla Rukmanawati Utiya Nabila Maulani Utiya Nabila Maulani Vita Pertiwi Vitri Widyaningsih Yulia Sari Yulia Sari Yulia Sari Yulia Sari Yuliana Heri Suselo Yunia Hastami Yunika Varestri Anugrah Rizki Yusman, Felizia Alika Zara Offia Sweetry Zuchaliya, Anifa Chofsoh