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Ahmadi Riyanto
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medpub@litbang.deptan.go.id
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INDONESIA
Jurnal Ilmu Ternak dan Veteriner
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary
Articles 6 Documents
Search results for , issue "Vol 22, No 4 (2017): DECEMBER 2017" : 6 Documents clear
Contamination of Escherichia coli O157:H7 in dairy cow farms Faidah Rachmawati; Tati Ariyanti
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.062 KB) | DOI: 10.14334/jitv.v22i4.1674

Abstract

E. coli serotype O157:H7 is a foodborne pathogen that endangers human health. Cattle are the main reservoir of this bacterium. E. coli O157:H7 that come out of the livestock can contaminate the surrounding environment, dairy products, meat, vegetable or fruit crops, so it can act as a source of infection and disease in humans. This research was conducted to identify the contamination of E. coli O157:H7 on dairy cow farms so the current situation is known. Sampling area for this research was dairy cow farm in Depok, Cianjur, Sukabumi, and Bandung. Types of samples taken are cow anal swab or fresh feces and livestock drinking water. Isolation of E. coli bacteria was done on mTSB media, agar MacConkey, and Eosin Methylene Agar. Identification of bacteria as E. coli O157:H7 was done by biochemical test and for serotype determination was used agglutination test using monospesifik antiserum for O157 and H7. The results of research showed that of the total 178 samples had 126 (70.8%) samples containing E. coli bacteria. Of the 126 samples containing E. coli bacteria, 94 (74.6%) were determined as E. coli serotype O157:H7. The presence of E. coli O157:H7 contaminants on dairy cow farms, their potential impacts on human health, prevention and control of the disease is discussed in this paper.
Effect of Bali cattle ovarian status on oocytes nuclear maturation and in vitro fertilization rate Herry Sonjaya; M. Yusuf; A. Hamdana; Renny Fatmyah Utamy; Sri Gustina; Hasbi Hasbi
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.074 KB) | DOI: 10.14334/jitv.v22i4.1585

Abstract

The aim of this study was to investigate whether the reproductive status influences the nuclear maturation and fertilization rates of bali cattle oocytes in vitro. Several pairs of ovary were classified into four groups: 1) ovaries with Corpus Luteum (CL) and Dominant Follicle (DF), 2) ovaries without CL and with DF, 3) ovaries with CL and without DF, 4) ovaries without both CL and DF. In the first experiment, oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) medium supplemented with 10% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in tissue culture medium (TCM)-199 supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), and 50 μg/ml gentamycin. Oocytes were matured in 5% CO2 incubator, 38oC for 24 h. In the second experiment, oocytes were matured and then fertilized in vitro to observe pronuclear formation. The first experiment showed that the percentage of oocytes reached methaphase-II (MII) stage on ovaries with CL and without DF (89.47%) were higher (P<0,01) compared to ovaries without both CL and DF (75,47%), ovaries without CL and with DF (74.,41%), or ovaries with CL and DF (65,52%). The result of second experiment showed that the ovarian reproductive status was not significantly different (P>0.05) on fertilization rate.
Supplementation of molasses and branched-chain amino acid to increase in vitro digestibility of ammoniated corn cob in ruminants feed Wisri Puastuti; Dwi Yulistiani; Eko Handiwirawan
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.856 KB) | DOI: 10.14334/jitv.v22i4.1664

Abstract

Corn cob contains high fiber and lignin which causes low nutritive value. The objective of the study was to improve the digestibility of ammoniated corn cob (CC) by supplementation of molasses and branched-chain amino acid (BCAA: valine, leucine and isoleucine). CC was processed by addition 3% urea. The first stage of in vitro test was done with 4 levels of molases 0, 5, 10 and 15% of dry matter (DM) of CC. The experiment was carried out using complete randomized design with 4 treatments and 4 replications. The second stage was also in vitro study of supplementation of two levels each for valine = V, leucine = L and isoleucine = I namely 0.1 and 0.2% of DM of ammoniated CC. There were 8 treatments combination of the BCAA as follow: A = V0.1 L0.1 I0.1; E = V0.2 L0.1 I0.1; B = V0.1 L0.1 I0.2; F = V0.2 L0.1 I0.2; C = V0.1 L0.2 I0.1; G = V0.2 L0.2 I0.1; D = V0.1 L0.2 I0.2; H = V0.2 L0.2 I0.2 and 1 control (V0.0 L0.0 I0.0). The experiments were done using completely randomized design with 9 treatments and 3 replications. The results showed that treated CC with urea was able to increase protein content by 78% (increased from 3.34% to 5.95%) while neutral detergent fiber (NDF) decreased by 15.4%, acid detergent fiber (ADF) by 7.9% and lignin 16.7%. Addition of molasses in ammoniated CC increased DM digestibility (P <0.05) by 7.5% (41.9 vs 43.51-46.26%) and NDF by 17.7% (38.41 vs 43.76 – 46.31%). Supplementation of BCAA resulted in the highest DM, OM and NDF digestibility (P <0.05) in the treatment of A, C, D and G. Compare to treament I, the digestibility of DM, OM and NDF in G treatment increased by 31.4%, 27.5% and 36.5%, respectively and produced the highest total population of rumen bacterial of 12.4 x 109 colonies /ml. It can be concluded that the digestibility of ammoniated CC increased by the supplementation of 5% molases and BCAA combination consisted of 0.2% valine, 0.2% leucine and 0.1% isoleucine. Kata kunci: Tongkol jagung, amoniasi, molases, asam amino bercabang.
Growth and feed utilization of Boer x Kacang crossbred goats offered total mixed rations of different protein and energy levels Simon P. Ginting; Kiston Simanihuruk; Antonius Antonius; Andi Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.915 KB) | DOI: 10.14334/jitv.v22i4.1782

Abstract

The aim of this study was to evaluate the growth of and feed utilization by Boer x Kacang crosses goats fed on total mixed ration differing in protein and energy levels. Four total mixed rations combination were formulated to contain 16 and 18% crude protein and 2650 and 2850 Kcal ME/ kg DM (dried matter) energy density. Twenty-eight male Boer x Kacang crosses goats (14.5 ± 1.14 kg) and of age ranging from 4 to 5 months were randomly allocated to one of these four TMRs (total mixed rations) (7 animals/TMR). The effects of dietary treatment were assessed using the general linear model and significance of the diet effects was detected using Duncan’s multiple range test. Dry matter intake increased as metabolizable energy density of diet increased from 2650 to 2850 Kcal/kg DM, but it is not affected by increasing crude protein level from 16 to 18%. The average daily gains were not improved (P>0.05) as the crude protein levels and metabolizable energy density of diet increased. Crude protein levels and ME density did not affect (P>0.05) the DM, OM and energy digestibility, but NDF digestibility was affected by the ME density of diets (P<0.05). Daily N intakes were greater (P<0.0%) in goats received diets higher in the crude protein and metabolizable energy levels. At this high rate of feed intake this type of goats are able to gain optimally when offered feed with crude protein level of 16% and metabolizable energy density of 2850 Kcal/kg DM.
Anticoccidial activity of Artemisinin and Extract of Artemesia annua leaves in chicken infected by Eimeria tenella Ening Wiedosari; April Hari Wardhana
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.668 KB) | DOI: 10.14334/jitv.v22i4.1622

Abstract

The continuous use of anticoccidial drug in chicken often continuously generates drug resistance and tissue residue; so thatconsequently, a safe alternative anticoccidial drug based on herb is fundamentally required. The aim of thise study was to examine anticcocidial activity of artemisinin and extract of Artemesia annua leaves in chicken infected by Eimeria tenella. A total of 35 chickens of Cobb strain was divided into seven groups with five replicates birds per group, i.e. uninfected chicken group (P I), infected but untreated chicken group (P II), infected and treated chicken group with 8.5 ppm, 17 ppm, 34 ppm, for P III, P IV and P V respectively, infected and treated chicken with 17 ppm of A. annua extract (P VI) and infected and treated chicken with Sulfa (P VII). All chicken, except the uninfected group, whereas infected with 2000 infective oocyst of E. tenella except the uninfected group. Treatment was delivered by oral, once per day for eight days. The criteria observed were clinical manifestation of chickens, number of oocyst in feces, body weight, cecal lesion score, haematocrit (packed cell volume) and haemoglobin value. The results showed that extract of A. annua leaves (P VI) was the most effective treatment to reduce the number of oocyst in feces (74.18%), followed by 34 ppm of artemisinin group (P VII). In addition, application of A. annua extract and artemisinin was significantly able to decreased the cecal lesion score (P<0.05). Even though body weight and Hb value were not indifferent significantly different (p>0.05), however A. annua extract and artemisinin treatments were significantly able to hold PCV value on normal level compared to P II and P IV (P<0.05). It concluded that extract A. annua leaves and artemisinin could be used an alternative anticoccidial in chickens.
Genetic polymorphisms of IGF1, GH, and OPN genes in crosses Peranakan Ongole cattle based on birth type in Central Java Anneke Anggraeni; Chalid Talib; Santi Ananda Asmarasari; Tati Herawati; Erik Andreas
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.152 KB) | DOI: 10.14334/jitv.v22i4.1625

Abstract

Genetic Polymorphisms of IGF1, GH, and OPN Genes in Crossbred of Peranakan Ongole Cattle Based on Birth Type in Central Java. Improved productivity by crossing Peranakan Ongole (PO) cattle to exotic beef cattle through AI mating is preferred by many farmers in Central Java. Fertility determines the ability of a cow to give birth.  IGF1 gene is predicted to affect either twin or multiple births in cattle, whereas GH and OPN genes determine reproductive fertility. Genetic polymorphisms of these three genes were studied in the crossbred of PO cattle for single birth (S) as well as twin and multiple (M) births or their historical (M) ones from two districts in Central Java, from Sragen (S = 7 hd and M = 13 hd.) and from Kendal (S = 9 hd, and M = 16 hd.).  Genetic polymorphisms were identified by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) method using restriction enzymes of SnaBI (IGF1 gene), MSpI (GH gene), and BsrI (OPN gene).  IGF1|SnaBI locus in the observed crossbred PO did not have SNP polymorphism or monomorphic for single and twins or multiple births.  In contrast, GH|MSpI and OPN|BsrI loci were polymorphic with quite a high degree of diversity.  In conclusion, the IGF1|SnaBI locus cannot be used to investigate possible genetic control of twins and multiple births, whilst GH|MSpI and OPN|BsrI polymorphisms can be considered as initial information for selection on fertility traits in beef cattle.

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