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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal International Journal of Social Science and Humanity
H. Hasbi
Department Of Animal Production, Faculty Of Animal Science, Hasanuddin University
Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Energy Languange, Linguistic, Communication & Media Social Sciences Veterinary

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OVARIAN RESPONSE OF DAIRY COWS TO PROGESTERONE COMBINED ON Toleng, A.L.; Yusuf, M.; Hasbi, H.; Putri, A.R.M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 3 (2013): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.3.156-162

Abstract

The objective of this study was to evaluate the response of ovaries in estrus synchronizationprotocol using GnRH-PGF2α based protocol combined progestin in a herd with tie-stall housing system.A total of 20 Holstein Friesian cows at different stages of estrus cycle in a dairy herd were enrolled inthe present study. The cows were randomly allocated into two treatment groups; with and without CIDR(Controlled internal drug release) insertion. All cows received 100 μg of GnRH I.M. at the beginning ofthe treatment (d 0) without regard to the stages of the estrus cycle followed by 25 mg PGF2α IM 7dayslater. Cows in CIDR group were inserted CIDR into the vagina at the time of GnRH administration andwere removed on the day of PGF2α administration. estrus signs were checked twice daily starting onday-2 after initiation of the protocol, except cows in CIDR group the estrus signs were checked startingon day-8 of the protocol. The animals showing estrus signs were noted. Blood samples were collectedfrom all animals on the days of GnRH and PGF2α treatments and on day-10 after initiation of protocolfor progesterone concentration. The results of this study showed that 30% animals in cows withoutCIDR insertion showed estrus signs prior to PGF2α injection. Involving CIDR to synchronized estruswith GnRH-PGF2α based protocol avoided the occurrence of premature estrus and maintained highprogesterone concentration on the day of PGF2α administration. It is concluded that ovarian responseafter involving CIDR in the protocol for estrus synchronization especially using combination of GnRHand PGF2α were much effective to synchronize the initiation of estrus in dairy cows. Likewise, the useof progesterone avoided premature estrus.
Insulin-Like Growth Factor-I Concentration in the Follicular Fluid of Bali Cattle and Its Role in the Oocyte Nuclear Maturation and Fertilization Rate H. Hasbi; S. Gustina; N.W. K. Karja; I. Supriatna; M. A. Setiadi
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.199 KB) | DOI: 10.5398/medpet.2017.40.1.7

Abstract

The objective of this study was to determine the concentration of IGF-I in the follicular fluid (FF) of Bali cattle and its role in the nuclear maturation and fertilization rate. The follicular fluid was collected by the aspiration technique, then it was centrifuged at 1500 g for 30 min at 24oC. The supernatant was collected and stored at -20oC until being used in the experiment for analysis of IGF-1. A total of 1105 oocytes were used in this study. The oocytes were matured in M199 without supplementation of bovine serum albumin, with supplementation of BSA, and with supplementations of 10% FF (v/v) from the follicle with diameter Ø<4 mm, 4≤Ø<6 mm, 6≤Ø<8 mm, and Ø≥8 mm at the luteal phase and then fertilized. The results showed that the concentrations of IGF-I in the FF obtained during the luteal phase was significantly higher (P<0.05) compared to those obtained during follicular phase. The IGF-I concentrations in the follicular fluid of follicle with diameter smaller than 6 mm were significantly higher (P<0.05) compared to those with diameters larger than 6 mm. The percentage of nuclear maturation rate of oocytes cultured with FF obtained from follicle with diameter <4 mm was significantly higher (P<0.05) compared to those obtained from the other groups of follicle diameters. The supplementation of maturation media with BSA and FF were able to improve fertilization rate significantly (P<0.05) compared to maturation media without BSA. In conclusion, the concentration of IGF-I in the follicular fluid obtained during the luteal phase was higher compared to those obtained during the follicular phase. The IGF-I concentrations in the follicular fluid of smaller follicles (diameter <6 mm) were higher compared to those in the large follicles (diameter ≥6 mm). The supplementation of FF can improve the nuclear maturation and fertilization rate
Characteristics of Libido and Testosterone Concentration of Polled and Horned Bali Bulls after GnRH Injection Hasbi Hasbi; Herry Sonjaya; Sudirman Baco; Reski Amalia; Sri Gustina
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2851

Abstract

Bali cattle are one of the original Indonesian germplasm, which in its development were found to be hornless or polled. Polled are Balinese cattle whose horns do not grow naturally, but have the same characteristics as those with horns. However, there are indications that polled have a lower libido. The purpose of this study was to determine the characteristics of libido and testosterone concentration after gonadotrophin releasing hormone (GnRH) injection. In this study each of 7 male polled and horned Bali cattle aged 3.5-6 years were used. Libido characteristics were observed by recording the first time the male fondled the teaser until ejaculation, while the testosterone concentration was measured using blood plasma taken on day 0 or before injection, day 7th, and day 14th after GnRH injection. Testosterone analysis used enzyme linked immunosorbent assay (ELISA) method. The results showed that the libido of polled was not different (P>0.05) compared to that of the horned on the 0, 7th and 14th days after GnRH injection. However, in polled on the 7th day after GnRH injection, it was significantly lower than on day 0 and 14, but on day 0 it was not different with day 14. Testosterone concentration on day 7 after injection of GnRH was not different (P>0.05) compared to day 0 both in polled and horned bulls, but on day 14 after injection was significantly higher (P<0.05) in horned than polled. In conclusion, polled and horned Bali bulls had high libido with a score of +1, but on the 7th day after GnRH injection, polled had lower libido than horns. The testosterone concentrations of polled and horned on day 7 after GnRH injection were not different compared to day 0, while the 14th day was higher in horned bull.
Effect of Bali cattle ovarian status on oocytes nuclear maturation and in vitro fertilization rate Herry Sonjaya; M. Yusuf; A. Hamdana; Renny Fatmyah Utamy; Sri Gustina; Hasbi Hasbi
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.074 KB) | DOI: 10.14334/jitv.v22i4.1585

Abstract

The aim of this study was to investigate whether the reproductive status influences the nuclear maturation and fertilization rates of bali cattle oocytes in vitro. Several pairs of ovary were classified into four groups: 1) ovaries with Corpus Luteum (CL) and Dominant Follicle (DF), 2) ovaries without CL and with DF, 3) ovaries with CL and without DF, 4) ovaries without both CL and DF. In the first experiment, oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) medium supplemented with 10% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in tissue culture medium (TCM)-199 supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), and 50 μg/ml gentamycin. Oocytes were matured in 5% CO2 incubator, 38oC for 24 h. In the second experiment, oocytes were matured and then fertilized in vitro to observe pronuclear formation. The first experiment showed that the percentage of oocytes reached methaphase-II (MII) stage on ovaries with CL and without DF (89.47%) were higher (P<0,01) compared to ovaries without both CL and DF (75,47%), ovaries without CL and with DF (74.,41%), or ovaries with CL and DF (65,52%). The result of second experiment showed that the ovarian reproductive status was not significantly different (P>0.05) on fertilization rate.
Oocytes Population and Development Competence of Bali Cattle Embryo In Vitro with Different Ovarian Reproductive Statuses H. Hasbi; H. Sonjaya; S. Gustina
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.389

Abstract

The present study aims to determine the potential of Bali cattle ovaries as sources of oocytes for in vitro embryo production based on different ovarian reproductive statuses. The ovaries were grouped into 4 categories: ovaries with no corpus luteum and dominant follicles (CL-DF-), those with corpus luteum and no dominant follicles (CL+DF-), those without corpus luteum but with dominant follicles (CL-DF+), and those with corpus luteum and dominant follicle (CL+DF+). The oocytes were collected via the slicing technique and grouped into 4 grades (a, b, c, and d). The oocyte’s maturation was performed using tissue culture medium 199 basic media. A drop sample (10–15 oocytes/drop) covered with mineral oil was then placed in a 5% CO2 incubator at a temperature of 38.5 ℃ for 24 h. Then, the samples were fertilized in 80 μL of fertilization medium with a final spermatozoa concentration of 1.5×106 spermatozoa/mL. After 5–6 h of in vitro fertilization, the oocytes were washed four times using the Charles Rosenkrans 1aa (CR1aa) medium. Then, the samples were cultured using the CR1aa as a base medium. The results showed no significant difference (p>0.05) for the 4 groups based on the oocyte population collected from one pair ovary as well as the number of oocytes that were suitable for maturation. However, group CL-DF+ showed a significant difference (p<0.05) in the rate of nuclear maturation (80.00±12.84), fertilization rate (80.00±4.72), and the ability of embryo development (60.19±22.45) when compared to group CL-DF-, CL+DF-, and CL+DF+. This study determines that the oocyte population of Bali cattle ovary pairs and oocytes quantity that are fit for maturation is not influenced by the reproductive status of the ovaries. However, the level of nuclear maturation, fertilization, and the ability of embryo development is higher in the ovaries without corpus luteum but with dominant follicles.
The Quality of Fresh and Frozen Semen and its Correlation with Molecular Weight of Seminal Plasma Protein in Bali Cattle H. Iskandar; H. Sonjaya; R. I. Arifiantini; H. Hasbi
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.405

Abstract

This study evaluated the quality of fresh and frozen semen of Bali cattle and its correlation with the molecular weight (MW) of seminal plasma protein. This study collected semen from 10 bulls aged 5–10 years using an artificial vagina and evaluated the samples macroscopically and microscopically. Two batches of frozen semen obtained in 2020 and 2021 were also analyzed. The frozen semen samples were thawed at 37 °C for 30 seconds. The sperm motility, viability, intact plasma membrane (IPM), and sperm abnormalities were investigated. The concentration of the seminal plasma proteins was determined using the Bradford method, and the proteins were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE). Additionally, the gels were stained with Coomassie brilliant blue, and the MWs of the proteins were determined using MW markers. The sperm motility, viability, and abnormalities of fresh semen varied significantly among the bulls (p<0.05); however, sperm IPMs among the bulls were similar (p>0.05). No differences in sperm motility after freezing were found among the bulls. However, the sperm viability, abnormality, and IPM varied among the bulls. Meanwhile, the seminal plasma proteins contained bands with different MWs. No difference in the expression of protein bands between bulls. Linearity analysis showed that sperm motility (r= 0.281), viability (r= 0.189), abnormalities (r= 0.141), and IPM (r= 0.173) were positively correlated with the protein bands at each MW (p<0.05). The results conclude there was a positive correlation between the MW of the protein marker and the same protein expression levels in Bali bulls. Therefore, the band intensity of Bali cattle seminal plasma proteins can be used as a biomarker for selecting superior Bali bulls.
Women's Opportunities to Migrate at a Young Age: Taking Advantage of the Momentum of Demographic Bonus Musrayani Usman; Hasbi Hasbi; Mario Mario; Ria Renita Abbas; Rahmat Muhammad
International Journal of Social Science and Humanity Vol. 1 No. 2 (2024): June : International Journal of Social Science and Humanity
Publisher : Asosiasi Penelitian dan Pengajar Ilmu Sosial Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/ijss.v1i2.22

Abstract

Indonesia is currently in a demographic bonus until 2035. Where the productive age shows that the number of young people in the age range of 15-29 in 2022 reaches 24.3 percent and this is the basic capital in development. There are several countries that have successfully managed the demographic bonus and become developed countries such as Japan, South Korea, China and Singapore. Based on modernization theory that economic development will affect the value system of society that leads to gender equality. The Demographic Bonus is a golden opportunity for a nation if utilized properly. But the question is whether Indonesia is ready to seize the opportunity. The purpose of this paper is how to reveal facts, phenomena, variables. The research method uses a literature study with a descriptive-qualitative analysis method based on literature review. The results of the study are that there are several reasons that encourage someone to migrate with economic, family, education and work motives. Currently, Indonesia faces challenges in human development. The arrival of young migrants will lead to population growth and human resource development. The decision to migrate between men and women has different driving and pulling factors, women's participation to migrate where women's labor force participation is only 53.41 percent compared to men's 83.87 percent.
Co-Authors A L Toleng A. Hamdana A.R.M. Putri Gustina, Sri H. Iskandar H. Sonjaya Herry Sonjaya I. Supriatna M. A. Setiadi M. Yusuf M. Yusuf Mario Mario Musrayani Usman N.W. K. Karja R. I. Arifiantini Rahmat Muhammad Renny Fatmyah Utamy Reski Amalia Ria Renita Abbas S. Gustina S. Gustina Sri Gustina Sudirman Baco