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Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy)

Farmasi
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Published by Universitas Wahid Hasyim Semarang

ISSN : 16937899 EISSN : 27163814 DOI : 10.31942

Core Subject : Health,

Medicine & Pharmacology

Selamat datang di situs e-Publikasi Ilmiah Fakultas Farmasi Unwahas (Universitas Wahid Hasyim) Semarang. Situs ini berisi kumpulan publikasi ilmiah yang diterbitkan oleh Fakultas Farmasi Unwahas. Publikasi berasal dari jurnal-jurnal serta hasil prosiding seminar yang dilaksanakan oleh Fakultas Farmasi Unwahas.

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AKTIVITAS ANTIBAKTERI SALEP EKSTRAK DAUN SUKUN (Artocarpus altilis) DENGAN DUA MACAM KOMBINASI BASIS SALEP TERHADAP BAKTERI Stapylococcus aureus Dewi Andini Kunti Mulangsri; Heru Fitranto; Yana Astiana; Mufrod Mufrod
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 16, No 02 (2019): Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 02 Desember 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT Breadfruit leaves (Artocarpus altilis) was contain compound of flavonoids, tannins and phenol that the ability as antibacterial against Staphylococcus aureus bacteria. The extracts of breadfruit leaves formulated became ointment that made practice to used. The combine of ointment bases can obtain of good consistency like the combine of adeps lanae with vaselin album and cera flava with vaselin album. The aims of this study to know the profile of antibacterial activity of the breadfruit leaves extract ointment with two kind of the combine of ointment bases. The breadfruit leaves extract has obtained by maceration method used etanol 70% solvents. The breadfruit leaves ointment was made by melting method with combination bases A is adeps lanae:vaselin album as F1 (13.5%:76.5%), F2 (45%:45%) dan F3 (76.5%:13.5%), while combination B is cera alba:vaselin album as F1 (85.5%:4.5%), F2 (81%:9%), F3 (76.5%:13.5%). antibacterial activity assay was used well diffusion method and analyzed descriptively. The research result that the all formulas has breadfruit flavor, smooth texture and brown color. Two variatons of combination bases ointment has antibacterial activity against Staphylcoccus aureus bacteri with formed of inhibition zone around the well. Keywords: Breadfruit leaves extract, ointment, antibacterial, combination of ointment bases

PENGGUNAAN ANTIBIOTIK PADA PASIEN BPJS DI IGD RSUD dr. GONDO SUWARNO KABUPATEN SEMARANG Rias Ayu Kartika; Maria Caecilia Nanny Setiawati; Erna Prasetyaningrum
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 19, No 1 (2022): Jurnal Ilmu Farmasi & Farmasi Klinik
Publisher : Universitas Wahid Hasyim Semarang

The antibiotics used for patients in the ER (emergency room) needs special attention, because the inappropriate use of antibiotics at the beginning of treatment can affect subsequent antibiotic therapy. This study intents to evaluate antibiotics used in BPJS patients in the emergency department of RSUD dr. Gondo Suwarno, Semarang Regency. This research was conducted using prescription data for BPJS patients at the ER at RSUD dr. Gondo Suwarno, Semarang Regency for the period January-June 2020. We used purposive sampling as a technic sampling and the data is collective retrospectively. The results showed that 30.64% of the total prescriptions for antibiotics were prescribed for 478 patients, most of whom were adult patients (26-45 years), which were 29.71%. Most route of administration is per oral (76.09%). Diagnosis of antibiotics, the most are trauma and open wounds (40.38%). 75.94% of antibiotics were given singly, most in order were ciprofloxacin (17.36%), amoxicillin (15.9%) and cefixime (13.81%) all orally. The most common combination antibiotics were oral amoxicillin and topical gentamicin (14.44%). All antibiotics prescribed were in accordance with the national formulary. According to the WHO criteria, the total drug prescribed per sheet and the percentage of antibiotic prescriptions for BPJS patients in the emergency room of RSUD dr. Gondo Suwarno, Semarang Regency, has not been rational, while the percentage of injection prescriptions, generic antibiotic prescriptions and conformity with National Formulary is rational.

UJI AKTIVITAS ANTIPIRETIK EKSTRAK DAUN SISIK NAGA(Pyrrosia piloselloides (L.) M.G. Price) TERHADAP TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI PEPTON 5% Ratna Widyasari; Dina Yuspitasari; Fadli Fadli; Athiah Masykuroh; Winda Tahuhiddah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 15 No 1 Juni 2018
Publisher : Universitas Wahid Hasyim Semarang

INTISARI Demam adalah keadaan ketika suhu tubuh meningkat melebihi suhu tubuh normal. Demam terjadi karena pelepasan pirogen dari dalam leukosit yang sebelumnya telah terangsang oleh pirogen eksogen yang dapat berasal dari mikroorganisme atau merupakan suatu hasil reaksi imunologik yang tidak berdasarkan suatu infeksi. Sementara itu, banyak negara telah mengembangkan pengobatan menggunakan herbal. Salah satu usaha yang dilakukan adalah dengan penggunaan obat tradisional. Penelitian ini bertujuan untuk membuktikan bahwa daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price) dengan konsentrasi 0,062%, 0,125% dan 0,25% memiliki aktivitas antipiretik dengan menggunakan penginduksi demam Pepton 5%. Penelitian ini merupakan penelitian eksperimental dengan Rancang Acak Lengkap (RAL). Pengukuran suhu rektal tikus menggunakan termometer infra red. Hewan uji dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (suspensi Na-CMC 1%), kelompok kontrol positif (suspensi parasetamol) dan kelompok uji yaitu pemberian ekstrak daun sisik naga 0,062%, 0,125% dan 0,25%. Data yang diperoleh dianalisis dengan menggunakan uji anova dan uji LSD (Least Significant Different). Hasil dari penelitian ini bahwa ekstrak daun sisik naga (Pyrrosia piloselloides (L.) M.G Price) konsentrasi 0,25% mempunyai aktivitas antipiretik pada tikus putih (Rattus norvegicus) jantan galur wistar. Kata kunci: Antipiretik, Daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price), Pepton 5% ABSTRACT A fever is a condition when the body temperature rises beyond the normal body temperature. Fever occurs due to the release of pyrogytes from within leukocytes that have previously been stimulated by exogenous pyrogens that may originate from microorganisms or are a result of immunologic reactions that are not based on an infection. Meanwhile, many countries have developed treatments using herbs. One of the efforts done is with the use of traditional medicine. This study aims to prove that the leaves of the Dragon Scales (Pyrrosia piloselloides (L.) M.G Price) with concentrations of 0.062%, 0.125% and 0.25% have antipyretic activity using Pepton fever induction 5%. This research is an experimental research with Completely Random Design (RAL). Rectal temperature measurements of mice using infra red thermometer. Test animals were divided into 5 groups: negative control group (1% Na-CMC suspension), positive control group (paracetamol suspension) and the test group were 0.062%, 0.125% and 0.25%. The data obtained were analyzed using anova test and LSD (Least Significant Different) test. The results of this study showed that the extract of leaf scales (Pyrrosia piloselloides (L.) M.G Price) concentrations of 0.25% had antipyretic activity in white rats (Rattus norvegicus) male wistar strain. Keywords: Antipyretics, Leaf Scales Dragon (Pyrrosia piloselloides (L.) M.G Price), Pepton 5%

EVALUASI IN VITRO-IN VIVO FILM TRANDERMAL DILTIAZEM HCL DENGAN PENINGKAT PENETRASI PEG 400 SEBAGAI ANTIHIPERTENSI Yulias Ninik Windriyati; Risha Fillah Fithria; Fitria Dwi Kurniawati; Ulfa Risalatul Mukaromah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 1 Juni 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACTDiltiazem HCL is an antihypertensive that low oral bioavailability of 40%, so developed to transdermal preparations. A matrix type of transdermal patch of diltiazem HCl was prepared using polyvinyl alcohol and ethyl cellulose with PEG 400 as penetration enhancer. In vitro-in vivo evaluation were conducted to asses drug permeation through the skin and determine the effectiveness of transdermal film as an antihypertensive drug. Transdermal patches of diltiazem HCl were evaluated for physicochemical characteristics weight variation, thickness, folding endurance, moisture uptake, and drug content. In vitro permeation study was conducted using commercial semi permeable membrane in Franz diffusion cell. In vivo activity study was evaluated on male rat Wistar that induced NaCl with CODA non-invasive blood pressure method. Transdermal patches of diltiazem HCl were found no significant differences in terms of physicochemical characteristics. The in vitro skin permeation profiles showed increased flux values with the increase of PEG 400 as a penetration enhancer. The in vivo evaluation showed a reduction in systolic and diastolic blood pressure within one hour after the drug administration. Diltiazem HCl was able penetration into skin, absorbed in blood circulation and effective as antihypertensive via transdermal route.Keywords : antihypertension, diltiazem HCl, PEG 400, transdermal patch

UJI EFEKTIVITAS EKSTRAK BUNGA KENOP (Gomprhrena globosa L) TERHADAP LUKA BAKAR PADA HEWAN UJI KELINCI (Oryctolagus cuniculus) Seri Agustini; Sari Wijayanti; Irma Novrianti
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 18, No 01 (2021): Jurnal Ilmu Farmasi & Farmasi Klinik
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT The plant that is believed by the public as a medicinal plant is Globe Amarant (Gomprena globosa L) which allegedly contains flavonoids and saponins compounds that can modulate wound healing acceleration. The flower has been shown to have an effect on wound healing in test animals, but so far there has been no research on the effectiveness of the knob flower on burns. Therefore, this study aims to identify the content of flavonoids and saponins in Kenop flowers and to see the effectiveness of healing burns in rabbits. The design of this study was an experimental study that used 5 groups of test animals, namely 2 control groups, and 3 groups of Kenop flower extract. Burn induction was performed using a 2x2 plate. The length of the wound was measured every 2 days using a caliper. Meanwhile, the identification test of flavonoid and saponin compounds was carried out using the forth and wilstatter methods. Based on the results of the study, it was found that the ethanol extract of the Kenop flower contains flavonoid and saponin compounds. The effectiveness test of the Knob flower extract on burns showed that the 30% extract group showed 92% wound healing with a wound diameter on the 14th day of 0.16; followed by a 20% extract group with a wound healing percentage of 84.3% with a wound diameter of 0.31 cm. The ethanol extract of the flower of the Kenop flower can heal burns at that concentration. Keywords: Kenop Flower, Flavonoids, Burns

UJI AKTIVITAS IMUNOMODULATOR FERMENTASI TEH HITAM JAMUR KOMBUCHA TERHADAP FAGOSITOSIS MAKROFAG MENCIT GALUR BALB/C SECARA IN VITRO Amalia Nurul ‘Ulum; Maria Ulfah; Ediati Sasmito
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 13 No 2 Desember 2016
Publisher : Universitas Wahid Hasyim Semarang

INTISARI Teh hitam dan kombucha adalah minuman kesehatan yang diketahui mampu meningkatkan sistem kekebalan tubuh. Penelitian ini bertujuan untuk membuktikan aktivitas imunomodulator fermentasi teh hitam jamur kombucha (FTHJK) terhadap fagositosis makrofag mencit galur Balb/C secara in vitro. Fermentasi kombucha dilakukan selama 5, 10 dan 15 hari menggunakan media seduhan teh hitam yang telah ditambahkan gula. Hasil FTHJK dibuat beberapa seri konsentrasi yaitu 6,25; 12,5; 25; 50, 100 µg/mL dan sebagai kontrol positif digunakan lipopolisakarida (LPS) 10 µg/mL untuk diujikan aktivitas fagositosis makrofag secara in vitro pada mencit jantan galur Balb/C usia 2 bulan. Data yang diperoleh berupa nilai Indeks Fagositosis (IF) (jumlah lateks yang dimakan oleh 100 sel makrofag) dan Kapasitas Fagositosis (jumlah sel makrofag yang memakan lateks). Hasil IF dan kapasitas dianalisis secara statistik menggunakan SPSS 16 for windows metode Friedman test, dilanjutkan uji Mann Withney. Hasil penelitian menunjukkan, FTHJK memiliki aktivitas imunomodulator terhadap peningkatan fagositosis makrofag pada konsentrasi 6,25; 12,5; 25; 50 dan 100 µg/mL dengan lama fermentasi 5, 10 dan 15 hari. Aktivitas imunostimulator tertinggi ditunjukkan pada konsentrasi 100 µg/mL dengan lama fermentasi 10 hari berdasarkan nilai IF. Kata kunci: Imunomodulator, IF (Indeks Fagositosis), Kapasitas Fagositosis, Fermentasi teh hitam jamur kombucha. ABSTRACT Black tea and kombucha is beverage drink was known that can increase immunity. This study aims to not only determine the fermented black tea kombucha as an immunomodulatory againts macrophages cell phagocytic of mice strains Balb/C in vitro, but also to determine concentration with duration fermentation that produces the highest activity Fermentation kombucha was carried out for 5, 10 and 15 days with steeping black tea has been given sugar. Fermented black tea kombucha was made to any series concentrations of 6,25; 12,5; 25; 50 and 100 µg/mL, as a positive control used lipopolysaccharide (LPS) 10 mg/mL there were tested in vitro on activity phagocytic macrophage cell of mice strains Balb/C. Value of Phagocytic Index (phagocytic of latex in 100 macrophage) and phagocytic capacity (amount of macrophage that phagocytic latex) were analyzed using SPSS 16 for windows with methode Friedman test followed by Mann Withney test. The results showed that fermented black tea kombucha has the highest as immunostimulatory activity on macrophage phagocytic at the concentration of 100 µg/mL and 10 days duration fermentation. Keywords: Immunomodulatory, Phagocytic Index, Phagocytic Capacity, Fermented black tea kombucha.

TOKSISITAS SUBAKUT EKSTRAK BIJI PEPAYA (Carica papaya L.) TERHADAP AKTIVITAS ENZIM Serum Glutamic Oxaloasetic Transminase (SGOT) MENCIT (Mus musculus) SECARA IN VIVO Safwan Safwan; Abdul Rahman Wahid; Ali Ridho Husein; Ni_Nyoman Mira Mentari
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 2 Desember 2017
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT The aim of this study was to investigate the subchronic toxicity of extract of papaya seed (Carica papaya L.) on rat by evaluate Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloasetic Transaminase (SGOT) activity. Twenty (20) white male miceswere randomly grouped into four groups (one control and three treatment groups). Group 1 received 1% of CMCNa. Group 2, 3, and 4 received extract of papaya seed at a dose of 50 mg/kg body weight, 500 mg/kg body weight, and 5000 mg/kg body weight. After 28 days treatment, blood samples were collected from jugular vein near the eye to evaluate SGPT and SGOT level in serum. Data were analyzed using ANOVA (at 95% confidence). It is observed that extract of papaya seed at a dose 50 mg/kg body weight, 500 mg/ kg body weight, and 5000 mg/kg body weight have no effect to increase SGPT and SGOT activity. Key Word : Papaya Seed,Subacute, Toxicity, SGPT, SGOT

RENDEMEN KITOSAN LIMBAH CANGKANG KERANG SIMPING (Amusium pleuronectes) DAN KERANG KEPAH (Polymesoda erosa) DARI KENDAL JAWA TENGAH Ariyanti Ariyanti; Eni Masruriati; Arin Widya Nuari; M. Himawan Yoga Syahputra
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 1 Juni 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACTChitosan is the result of the deacetylation process of chitin by removing acetyl groups to obtain polymer compounds and glucosamine. Utilization of chitosan derived from waste of Amusium pleuronectes and Polymesoda erosa has not been maximize. This study aims to determine the highest chitosan yield in Amusium pleuronectes and Polymesoda erosa. The method used in this study was a deacetylation isolation method with five replications. The yield of the yield content was analyzed by univariate and bivariate with spps 19.0 using a ttest. Based on the results of the research on the sample of Amusium pleuronectes and Polymesoda erosa, the highest yield of chitosan at 75% b/v concentration of NaOH was 6,972% b/v for Amusium pleuronectes and 5.972% b/v for Polymesoda erosa. The results showed that NaOH concentrations of 15v, 25, 50, and 75% b/v could affect the chitin change to chitosan against Amusium pleuronectes and Polymesoda erosa.Keywords: Amusium pleuronectes, Polymesoda erosa, deacetylation, chitin, chitosan

STATINS DRUG USE AND DRUG-DRUG INTERACTIONS Maria Caecilia N.Setiawati; Caecilia Mutiarawati; Uswatun Chasanah; Lina Fatin Fauziyyah
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 17, No 2 (2020): Jurnal Ilmu Farmasi & Farmasi Klinik Vol. 17 N0. 02 Desember 2020
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT Dyslipidemia is the commonest cause of many atherosclerotic diseases. Statins are the mainstay of the management of dyslipidemia, and it is widely prescribed for patients in Indonesia. This study aims to give an overview, the use of statin, and its drug-drug interactions. The study method was descriptive, using a purposive sampling technic. The inclusion criteria were patients who received statins therapy, in the outpatient installation of Tugurejo Regional Public Hospital, Semarang, during April 2017. There were 334 patients meet the inclusion criteria, most of them were women (63,28%). The highest patients’ range was 60-69 years old of 129 patients (38,62%). One hundred and thirty-one patients (39,22%) were diagnosed with dyslipidemia only. The dosage range of simvastatin and rosuvastatin was 10 to 20 mg once daily, but rosuvastatin was only given 10 mg once daily. The most dose was 10 mg simvastatin per day, prescribed for 231 patients (69,16%). There were 177 patients (52,99%) who has the potentiate of statin drug interactions. The most were simvastatin-amlodipine interactions, occurs in 104 patients (31,14%). This study shows that most statin users are elderly patients. Statin is used not only in dyslipidemia patients. There are many potential statin drug-drug interactions, but the statin dose is low and not over the standard doses. Keyword: dyslipidemia, statins, drug interactions

PERBANDINGAN METODE PENETAPAN KADAR SIMETIDIN MENGGUNAKAN SPEKTROFOTOMETRI UV DAN KROMATOGRAFI CAIR KINERJA TINGGI Aqnes Budiarti; Adina Fitria K.W.; Sumantri Sumantri
Jurnal Ilmu Farmasi dan Farmasi Klinik JURNAL ILMU FARMASI & FARMASI KLINIK VOL. 13 NO. 1 JUNI 2016
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT Determination ofdrug substance is one of the drug quality control to ensure the safetyof drug. Determination of cimetidine could use UV spectrophotometry and HPLC. The aim ofthis study were to validate the methode using two instruments, to compare both of the method and to apply it intablet dosage forms. Determination of cimetidin using UV spectrophotometry was set on wave length 219 nm. Determination using HPLC with coloumn C18 and mobile phase mixture of methanol: water-phosphoric acid (30:70, v/v), flow rateat 1mL/min and UV detector. The method was validated in terms of accuracy, precision, linearity, selectivity and sensivity. The method of determination of cimetidine using both instruments were campared by analysis of variant. Validation method using UV spectrophotometry showed precision of 0.94%, recovery from 97.50 to 100.91%, good linearity, LOD 0.76 µg/mL and LOQ 2.52µg/mL.Validation using HPLC method resulted value of precision 0.30%, recovery from 98.42 to 101.83%, good linearity, LOD and LOQ of 0.46 and 0.56 µg/mL. The average concentration of cimetidine in tablets by spectrophotometry UV was 101.95%, while in HPLC was 99.69%.The methods accomplied to the requirements according The Indonesian Pharmacopoeia Edition IV. Both methods provided the same results and did not significantly different. Keyword : validation method, cimetidine, UV spectrophotometry, HPLC

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