cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 240 Documents
 20   21   22   23   24 
Stomata characters of sugarcane (Saccharum officinarum L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia Mahfut Mahfut; Putri Kendari; Admi Syarif; Sri Wahyuningsih; Endah Susiyanti
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79860

Abstract

The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding. 
The Synergistic Effect of Combination of Pentagamavunone-1 with Diosmin, Galangin, and Piperine in WiDr Colon Cancer Cells: In vitro and Target Protein Prediction Muthi Ikawati; Hajidah Musyayyadah; Yurananda Magnalia Putri; Ummi Maryam Zulfin; Febri Wulandari; Dyaningtyas Dewi Pamungkas Putri; Edy Meiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.80975

Abstract

Pentagamavunone-1 (PGV-1) is a curcumin analog with a prominent anti-cancer potency in vitro and in vivo for several cancer types, including colon cancer. Combining PGV-1 with natural compounds such as diosmin, galangin, and piperine can enhance its effectiveness due to their promising chemoprevention properties. We aimed to evaluate the effectiveness of combining PGV-1 with diosmin, galangin, or piperine for colon cancer by using in vitro and bioinformatic approaches to predict their target proteins. WiDr cells were used as a model for colon adenocarcinoma (COAD). The cell viability under a single or combination treatment of PGV-1 and diosmin, galangin, or piperine was evaluated using direct counting by the trypan blue exclusion test. SwissTargetProtein, UALCAN, and OncoLnc were utilized to predict target proteins of the compounds in COAD, the expression level of target proteins in COAD, and the survival rate of patients with overexpressed target proteins, respectively. The IC50 values for PGV-1, diosmin, galangin, and piperine were 2.8´10-2 µg/mL, 81 µg/mL, 7 µg/mL, and 172 µg/mL, respectively. All the tested natural compounds showed synergistic effects when combined with PGV-1 at low concentrations. Eleven proteins that were overexpressed in COAD were identified as potential targets. Overlapped predicted targets of PGV-1 and galangin or piperine were CDK1, MET, and TOP2A. The high expression of another set of predicted target proteins, SCD, CA9, and SQLE, led to lower survival rates in COAD patients. We concluded that combinations of PGV-1 with natural compounds can synergistically enhane its anti-cancer activity for colon cancer.
Nutritional Contents and Bioactive Compounds among Several Variants of Dolichos lablab: Fundamental Facts for Functional Food Development Elly Purwanti; Feri Eko Hermanto; Wahyu Prihanta; Tutut Indria Permana; I Gusti Ngurah Agung Wiwekananda
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.81339

Abstract

To date, the data describing various nutritional and secondary metabolites content of Lablab beans is incomplete. Therefore, this study evaluated the nutritional value, secondary metabolites, and antioxidant activity of three different variants of Lablab beans, i.e., brown, black, and cream beans. The results showed that the brown Lablab beans had outperformed other variants according to their nutritional value and flavonoid content with outstanding DPPH scavenging activity. However, the black beans also showed good bioactive contents through their total phenolic percentage with decent reducing activity via the FRAP assay. Those who are keen in developing functional food from Lablab beans should consider this data as a reference. 
Autecology of Nepenthes spp. in Peat Swamp and Heath Forest Pematang Gadung, West Kalimantan Nofi Utari; Sulistijorini Sulistijorini; Nunik Sri Ariyanti
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.81351

Abstract

Nepenthes occur in various habitat types in West Kalimantan. But some species are categorized as vulnerable on the IUCN red list. Autecological studies of Nepenthes spp. are required for species management and conservation. The study aimed to analyze species diversity, distribution pattern, association, and environmental factors that correlated with Nepenthes spp. at peat swamp and heath forest in Pematang Gadung. Data was collected in September 2021 by purposive sampling on 60 plots measuring 3 x 3 m2 in each habitat type. Species diversity was analyzed based on the presence and abundance of species in the plots. The distribution pattern was determined using a standardized Morisita index (Ip). Meanwhile, the association type was determined based on the Jaccard index (JI). Environmental data were analyzed by Canonical Correspondence Analysis (CCA) using Past Version 4.03. This study found six wild species in a peat swamp and four species in a heath forest. The population of Nepenthes spp. has clumped distribution pattern with Ip>0. Nepenthes spp. are associated with plants such as Barringtonia racemosa, Syzygium sp., Nephrolepis biserrata, and Campnosperma auriculatum. The result of CCA revealed that environmental factors in both habitat types affected the presence of each Nepenthes species. The air temperature, humidity, soil moisture, and light intensity show different influences on different species.  
Biostratigraphy and Climate Change in the Late Miocene Age Based on Foraminifera in the Oyo Formation, Oyo River Section, Gunung Kidul, Yogyakarta Adesti Audina Ulfah; Akmaluddin Akmaluddin; Didit Hadi Barianto
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.81769

Abstract

The date of the paleoclimate event was ascertained using a biostratigraphic analysis. The thickness of the Oyo Formation was measured using measurements with a Jacob stick method, yielding a thickness of 80.8 meters and 23 rock samples. In the Southern Mountains Zone,  new result of the age of the upper part of the Oyo Formation by biostratigraphy investigation of the hill Late Miocene (9.79 Ma to 5.78 Ma). Biostratigraphic investigation in the Oyo River revealed 28 species and 9 genera, with two datums. The study area was classified into three biozonations based on the datum found Globorotalia acostaensis/M13a/N16 zone, the lower Globorotalia plesiotumida/M13b/N17 zone, and the upper Globigerinoides conglobatus/M14/N17 zone. The results of a paleoclimate analysis on the Oyo River Section show a general cooling tendency in the study area. Seven paleoclimate zones can be determined from these trends consisting of four warm and three cold zones. Zone I (warm), zone II (cold), zone III (warm), and zone IV (cold) have the coldest peak in the study region in 8.3 Ma, zone V (warm), and zone VI (cold), followed by zone VII (warm). Based on the correlation with other studies (South China Sea, Pacific Ocean, Oyo River, Ngalang River, and Ngioro Section), paleoclimate events in the study area occur globally. 
Cobalamin and Thiamine Effect on Microalgae Biomass Production in the Glagah Consortium Tri Wahyu Setyaningrum; Arief Budiman; Eko Agus Suyono
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.81949

Abstract

The Glagah consortium is a mixed culture of various microalgae and bacteria isolated from Glagah Beach, Yogyakarta. Cobalamin and thiamine, which are given by symbiotic bacteria, are assumed will increase biomass. This study aimed to determine the effect of cobalamin and thiamine on microalgae biomass production in the Glagah consortium. The microalgae of Glagah consortium were cultivated for 10 days with vancomycin and gentamicin antibiotic as treatment and without antibiotics as a control. The parameters measured included the number of bacterial colonies, cobalamin and thiamine levels measured by LC-MS, chlorophyll a and b levels, cell density of microalgae and dry biomass. The highest level of cobalamin and thiamine was in the Glagah consortium without antibiotics. Cobalamin and thiamine increased in the exponential phase along with the increasing Staphylococcus sp. colonies. The Quantity of Staphylococcus sp. colonies in the exponential phase was 62.105 (cfu/mL). The level of cobalamin in the exponential phase was 2.33 µg/L and the level of thiamine in the exponential phase was 49.71 µg/L. The highest productivity dried weight biomass was 0.0134 g/L/day in the day-6th on the Glagah consortium without antibiotics. This result showed that microalgae and bacterial interaction was mutualism symbiosis involving cobalamin and thiamine that increased in the exponential phase along with the increasing Staphylococcus sp. colonies. This interaction was able to increase biomass microalgae.
Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker Suyono Suyono; Imey Tamara Indivia; Ruri Siti Resmisari; Fitriyah Fitriyah; Didik Wahyudi
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82238

Abstract

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation. 
In silico Screening of Potential Antidiabetic Phenolic Compounds from Banana (Musa spp.) Peel Against PTP1B Protein Rico Alexander Pratama; Junaida Astina; Arli Aditya Parikesit
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83124

Abstract

Type 2 diabetes mellitus (T2DM) is a global problem with increasing prevalence. The current treatments have made an immense progress  with some side effects, such as drug resistance, acute kidney toxicity, and increased risk of heart attack. Banana (Musa spp.) peel comprises 40% of banana fruit contains high phenolic compounds whilst some studies have suggested a correlation between phenolic compounds and antidiabetic activity. One of the novel protein targets that has been identified as a potential anti-diabetic treatment is PTP1B (PDB ID:2NT7). Therefore, this study aimed to screen the potential PTP1B inhibitor for antidiabetic treatment from phenolic compounds in banana peel. QSAR, molecular docking, ADME-Tox, and molecular dynamics analysis were deployed to examine forty-three phenolic compounds in banana peel. Eighteen ligands were screened by QSAR analysis and eight of them had a lower binding energy than the standard (ertiprotafib) in molecular docking, with urolithin A and chrysin were the lowest. Both passed Lipinski’s rule of five, had a good intestinal absorption, and no blood-brain barrier penetration, however, their mutagenicity, carcinogenicity, and irritation to the skin and eyes were still in questions. Molecular dynamics analysis found both of them were in a stable conformation with PTP1B. This study suggested a potential of urolithin A and chrysin as PTP1B inhibitor for antidiabetic treatment. Additionally, further experimentation is required to validate this finding.  
In Silico Analysis of Phalaenopsis Orchid Homeobox1 (POH1) Functional Gene for Shoot Development in Phalaenopsis Orchid Nuzlan Rasjid; Febri Yuda Kurniawan; Saifa Usni Putri; Aviesta Linggabuwana; Ireneus Seno Prasojo; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83934

Abstract

The most favorite ornamental crop in Indonesia is orchid which benefited as floriculture. Therefore, the quality of this crop must be improved.  Biotechnology is appropriate to be used to improve the quality and quantity of orchid plants. To conduct this method, researchers must know what genes function in plant development. In Phalaenopsis orchids, the gene has been identified as homeobox genes called Phalaenopsis Orchid Homeobox1 (POH1). This research aims to conduct in silico analysis of the gene. The materials were retrieved from mRNA and amino acid databases. Then, the materials are aligned, visualized, motif location analysis, motif function discovery, phylogenetic construction, and protein 3D structural modelling. Based on mRNA and amino acid alignment, there are 4 domain regions that are conserved in POH1 and other homologous genes, such as KNOX1, KNOX2, ELK Domain, and Homeobox KN Domain, which roles as a transcription factor involved in plant development. SWISS-MODEL and ColabFold were used in protein modelling of the protein. By ColabbFold modelling, the modelling prediction uses 325 residues, higher than SWISS-MODEL in 59 residues. ColabFold validation by Ramachandra Plot depicts having the most favourite regions is 68.6%, while SWISS-MODEL is 92.3%. Another validation parameter is overall quality factor and QMEAN Score. Protein modelling by ColabFold has overall quality factor 89.252 and QMEAN Score 0.41 ± 0.05. However, SWISS-MODEL 3D prediction has overall quality factor 98.039 and QMEAN score of 0.71 ± 0.11. 
Notes on The Current Distribution and Abundance of The Frog Genus Leptophryne spp. (Anura: Bufonidae) in Gede Pangrango National Park Mohamad Isnin Noer; Ratih Tryas Intani; Priya Yuga Prasetya; Alvita Dwi Lestari; Boby Darmawan
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): In progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84031

Abstract

We reported the current distribution of Leptophryne spp. in Gede Pangrango National Park. Leptophryne cruentata was recorded in Cibodas (Cikundul waterfall and Goa Lalay), Selabintana (Cibeureum Waterfall), and Goalpara (Rasta Waterfall), whereas Leptophryne borbonica was only reported from a creek at lowland forest of Bodogol. Goa Lalay and Rasta waterfall were explicitly reported as new distribution locations of Leptophryne cruentata. It was strictly found at a higher elevation usually within reach of waterfalls that generate substantial background noise, except in Cibodas in which Leptophryne cruentata can also be found in two noisy creeks located at a fairly great distance from waterfalls (100 – 170 meters). 

Page 24 of 24 | Total Record : 240

 20   21   22   23   24 

Filter by Year

2016 2023


Reset
Filter By Issues
All Issue Vol 8, No 3 (2023): In progress Vol 8, No 2 (2023): August Vol 8, No 1 (2023): April Vol 7, No 3 (2022): December Vol 7, No 2 (2022): August Vol 7, No 1 (2022): April Vol 6, No 3 (2021): December Vol 6, No 2 (2021): August Vol 6, No 1 (2021): April Vol 5, No 3 (2020): December Vol 5, No 2 (2020): August Vol 5, No 1 (2020): April Vol 4, No 3 (2019): Desember Vol 4, No 2 (2019): August Vol 4, No 1 (2019): April Vol 3, No 3 (2018): December Vol 3, No 2 (2018): August Vol 3, No 1 (2018): April Vol 2, No 2 (2017): December Vol 2, No 1 (2017): June Vol 1, No 2 (2016): December Vol 1, No 1 (2016): June More Issue