Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

INDONESIAN JOURNAL OF PHARMACY

3
Website

Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

Arjuna Subject : -

Articles 6 Documents

Search results for , issue "Vol 13 No 3, 2002" : 6 Documents clear

GENERAL ASPECTS OF PHOTOCHEMISTRY TO HUMAN Mursyidi, Achmad
Indonesian Journal of Pharmacy Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Photochemistry, the study of chemical reaction initiated by light plays an important role to human lifes. Photochemical reaction is intiated by the absorption of light giving excited molecules. The excited molecules, depending upon their structures and environment, may undergo various chemical reactions. Some very important and beneficial photochemical reactions occur in human body such as photochemical reaction of vision, the formation of vitamin D, and psoriasis medication. On the other hand, some photochemical reactions result in harmful effect to human, for examples, skin ageing, skin cancer, and cornea damage. Photosynthesis and photodegradation of pesticides are examples of photochemical reaction in the environment which are beneficial to human ienterset. In addition photochemical concept has been employed to synthesise drugs, nylon, and alkane-sulphates (detergents). However, some photochemical reactions create problem in the environment, i.e., formation of photochemical smogs, ozon layer depletion, and photodegaradion of drugs All those aspects of photochemistry are illustrated in this article.Key words: photochemistry, excited molecule, photodegradation, photooxidation, photosynthesis.

PRELIMINARY STUDY ON BIOSYNTHESIS, ISOLATION AND PURIFICATION OF L-35S-METHIONINE LABELLED COMPOUNDS Oekar, Nanny Kartini
Indonesian Journal of Pharmacy Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The preliminary study of biosynthesis sulphur-35 amino acids labelled compounds has been done using wild strain of Baker's yeast Saccharomyces cerevisiae as producer mycroorganism and the solution of Na35SO4 (45 mCi/60 L) as tracer source was added into the salt media, in which the incorporation of S-35 radioisotope to the yeast cells was synthesized. Maximum incorporation of 93,5 % was obtained after 10 hours incubation at 30 C. Hydrolysis was carried out using HCl 6N and heating at 110 C for 18 hours to destroy the yeast cells, therefore L-35-methionine and the other amino acids would dissolve in the solution. Hydrolisate isolation was exhibited using two step of coloumn chromatographic using Dowex-cation exchanger resin as stationary phase and the demineralized steril water containing 0, 1 % -mercapto ethanol (ME) and the mixing of water: HCl 4N which contains 0, 1% ME as mobile phase. The ratio volume of these both mobile phases were gradually adjusted. Purified amino acids the was analysized using paper chromatography method. The result shows that the 7th fraction of eluate contained L.35S-methionine biosynthesized by yeast Saccharomyces cerevisiae, that had high radiochemical purity (100 %) and the radioactive concentration of L-35S-methionine was 28 mCi/ml.Key-words: amino acids, biosynthesis, sulphur-35, L-35-S-methionine. Pertanian Kerjasama Dengan Kanwil Deptan dan Disbun Sumbar, Riau dan Jambi di Bukit Tinggi 15 - 17

COBALT INDUCTION ON BIOSYNTHESIS OF CYANOCOBALAMIN by Streptomyces olivaceus IFO 3409. Kasanah, Noer; Tunjung Pratiwi, Silvia Utami
Indonesian Journal of Pharmacy Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The structure of cyanocobalamin containing an essensial cobalt is as part the compound. The aim of this study was to investigate the influence of cobalt on biosynthesis of cyanocobalamin done by Streptomyces olivaceus IFO 3409. This experiment was carried out by cultivating S. olivaceus in fermentation medium contained glucose 1,5%, pepton 1%, yeast extract 0,25%, trace mineral and each of medium was added cobalt 5, 10, 20 ppm. Cyanocobalamin synthesized by the cells was harvested on the last exponential phase. The amount of cyanocobalamin was analyzed by spectrophotometer. The results show that no cyanocobalamin was produced by S. olivaceus culture without kobalt or treated with 5 ppm cobalt on medium. However cyanocobalamin was produced if the culture medium was treated with 10 and 20 ppm cobalt respectively. It was concluded that cobalt induced on cyanocobalamin biosyntesis, therefore optimation of adding kobalt might be examined on getting maximal cyanocobalamin produced by Streptomyces olivaceus IFO 3409.Key words: cyanocobalamin, cobalt, Streptomyces olivaceus IFO 3409.

THE INFLUENCE OF DISTILATION TIME, POWDER SIZE, ON THE CINAMYLALDEHYDE CONTENT AND VOLATILE OIL Cinnamomum burmanii Nees ex. Bl. BARK Lisawati, Yovita; Sulianti, Sri Budi; ., Chairul
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The content of volatile oil in samples depends on the harvest time, distilation method, sample powder size, and location of sample cultured. The aim of this study was to investigate the influences of interval time of distillation and mesh size of bark powder, upon the concentration of major component (cinamylaldehyde). The distillate was collected with various interval times 1, 2, 3 and 4 hours and it was counted after the first drop of distillate. The mesh size of bark powder (4, 8 and 20 mesh) was set after the interval time of distillation had been studied. The major component was analysed using Gas Chromatography (GC). The results showed that the interval time of distillation at the second hour gave the highest volatile oil and cinamylaldehyde contents in the relative prosentage 0.19 % and 29.36 % respectively, compared with the first hour distilate, (0.16 %, 24.88 %), third hours distilate (0.14 %, 23.29 %) and fourth distilate (0.08 %, 17.65 %). The powder size of bark (8 mesh) gave the highest volatile oil and cinamylaldehyde contents (0.22 %, 32.81 %), compared to powder size of 4 mesh( 0.17 %, 20.45 %) and 20 mesh (0.19 %, 21.32 %). The results is still lower than standard of cinnamon oil, (cinamylaldehyde content 60 %). The lowering cinamylaldehyde content could be caused by low quality of material, collected from marketing method, early harvesting time, and storing for long.time.Key words: C. burmanii, bark, distilation time, cinamylaldehide

ANTIINFLAMMATORY EFFECTS OF Cinnamyl tiglate CONTAIN IN VOLATILE OIL OF KUNYIT (Curcuma domestica Val.) Oktavia Salasia, Sri Isrina; ., Rochmadiyanto; Fatimah, Oktarina
Indonesian Journal of Pharmacy Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The aim of this study was to evaluate the antiinflammatory effects of the cinnamyl tiglate contain in the volatile oil of kunyit (Curcuma domestica Val.) on the carrageenin induced inflammatory in Wistar albino rats (Rattus norvegicus). Cinnamyl tiglate was isolated using thin layer chromatography with the silica gel GF as stationary phase and the hexane with toluene as a mobile phase. Cinnamyl tiglate could be isolated using gas chromatography and mass spectrometry (GCMS). Inflammation of rats were induced by 1% carrageenin subplantar followed with oral administration of the cinnamyl tiglate at various doses of 1,1%, 4,4%, and 17,6%/kg bw. As controle were used rats treated orally with aspirin 450 mg/kg bw and volatile oil of kunyit 1,2 ml/kg bw. The volume of inflammations were measured with pletismograph. The analysis of GCMS showed that cinnamyl tiglate could be isolated from volatyle oil of kunyit in the second fraction with the concentration of 63,62%. Cinnamyl tiglate with dose of 1,1%/kg bw had no effects on the inflammatory reactions (P≥0,05). Cinnamyl tiglate with dose of 4,4%/kg bw could decrease the inflammatory reactions (P≤0,05) and with dose of 17,6%/kg bw the antiinflammatory effects were more significant (P≤0,01).Key words: Cinnamyl tiglate, Curcuma domestica Val., antiinflammatory

GREEN FLUORESCENT PROTEIN (GFP), SUATU SIGNAL PENANDA QUANTITATIF UNTUK MEMONITOR PROLIFERASI SEL Puji Astuti; Leonore de Boer; Noelle-Anne Sunstrom; Peter P. Gray
Indonesian Journal of Pharmacy Vol 13 No 3, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Banyak penelitian sel kultur yang melibatkan sejumlah besar sampel untuk dianalisis, baik yang berhubungan dengan pertumbuhan sel atau toksisitas senyawa terhadap sel. Green Fluorescent Protein (GFP) adalah suatu protein yang secara alami dapat berfluorescence dan banyak digunakan pada berbagai aplikasi seperti penanda untuk ekspresi gena, produksi heterologous protein atau monitoring efisiensi transfeksi. Penelitian ini bertujuan untuk membandingkan tingkat akurasi, taraf kepercayaan dan reprodusibilitas GFP untuk memonitor pertumbuhan sel.Kurva baku dibuat dengan serial dilusi sel CHO-K1-EGFP dalam media 10% FCS di 96 well plate. Jumlah sel dalam tiap sumuran dihubungkan dengan signal fluorescence. Untuk memonitor pertumbuhan sel, signal fluoresensi dibandingkan dengan metode Trypan Blue Exclusion yang jumlah sel dalam tiap sumuran dihitung selama periode waktu tertentu (n=3). Untuk monitoring pertumbuhan sel, signal dari GFP memperlihatkan korelasi yang baik dengan jumlah sel dengan tingkat linieritas 0,9866 dalam kisaran jumlah sel 1250 – 1 x 105 sell/sumuran (standar error maksimum 11%). Metode ini terbukti memungkinkan pengukuran langsung signal fluoresensi sehingga mampu menurunkan kemungkinan kesalahan yang terjadi pada saat preparasi sel yang dapat mempengaruhi akurasi data yang diperoleh. Sekali klones permanen (stable clones) diperoleh klons ini dapat digunakan untuk banyak aplikasi.Kata Kunci: Green Fluorescent Protein (GFP), Fluorescence, proliferasi sel, Trypan Blue Exclusion

Page 1 of 1 | Total Record : 6

Filter by Year

2002 2002

Filter By Issues

All Issue Vol 31 No 2, 2020 Vol 31 No 1, 2020 In Press Vol 31 No 1, 2020 Vol 30 No 4, 2019 Vol 30 No 3, 2019 Vol 30 No 2, 2019 Vol 30 No 2, 2019 Vol 30 No 1, 2019 Vol 30 No 1, 2019 Vol 29 No 4, 2018 Vol 29 No 4, 2018 Vol 29 No 3, 2018 Vol 29 No 3, 2018 Vol 29 No 2, 2018 Vol 29 No 1, 2018 Vol 28 No 4, 2017 Vol 28 No 4, 2017 Vol 28 No 3, 2017 Vol 28 No 3, 2017 Vol 28 No 2, 2017 Vol 28 No 2, 2017 Vol 28 No 1, 2017 Vol 27 No 4, 2016 Vol 27 No 4, 2016 Vol 27 No 3, 2016 Vol 27 No 3, 2016 Vol 27 No 2, 2016 Vol 27 No 2, 2016 Vol 27 No 1, 2016 Vol 27 No 1, 2016 Vol 26 No 4, 2015 Vol 26 No 4, 2015 Vol 26 No 3, 2015 Vol 26 No 3, 2015 Vol 26 No 2, 2015 Vol 26 No 1, 2015 Vol 26 No 1, 2015 Vol 25 No 4, 2014 Vol 25 No 4, 2014 Vol 25 No 3, 2014 Vol 25 No 3, 2014 Vol 25 No 2, 2014 Vol 25 No 1, 2014 Vol 25 No 1, 2014 Vol 24 No 4, 2013 Vol 24 No 4, 2013 Vol 24 No 3, 2013 Vol 24 No 3, 2013 Vol 24 No 2, 2013 Vol 24 No 2, 2013 Vol 24 No 1, 2013 Vol 24 No 1, 2013 Vol 23 No 4, 2012 Vol 23 No 3, 2012 Vol 23 No 2, 2012 Vol 23 No 2, 2012 Vol 23 No 1, 2012 Vol 23 No 1, 2012 Vol 22 No 4, 2011 Vol 22 No 4, 2011 Vol 22 No 3, 2011 Vol 22 No 3, 2011 Vol 22 No 2, 2011 Vol 22 No 2, 2011 Vol 22 No 1, 2011 Vol 21 No 4, 2010 Vol 21 No 4, 2010 Vol 21 No 3, 2010 Vol 21 No 2, 2010 Vol 21 No 2, 2010 Vol 21 No 1, 2010 Vol 21 No 1, 2010 Vol 20 No 4, 2009 Vol 20 No 4, 2009 Vol 20 No 3, 2009 Vol 20 No 3, 2009 Vol 20 No 2, 2009 Vol 20 No 1, 2009 Vol 20 No 1, 2009 Vol 19 No 4, 2008 Vol 19 No 3, 2008 Vol 19 No 3, 2008 Vol 19 No 2, 2008 Vol 19 No 1, 2008 Vol 19 No 1, 2008 Vol 18 No 4, 2007 Vol 18 No 3, 2007 Vol 18 No 3, 2007 Vol 18 No 2, 2007 Vol 18 No 1, 2007 Vol 17 No 4, 2006 Vol 17 No 3, 2006 Vol 17 No 3, 2006 Vol 17 No 2, 2006 Vol 17 No 2, 2006 Vol 17 No 1, 2006 Vol 17 No 1, 2006 Vol 16 No 4, 2005 Vol 16 No 4, 2005 Vol 16 No 3, 2005 Vol 16 No 2, 2005 Vol 16 No 2, 2005 Vol 16 No 1, 2005 Vol 16 No 1, 2005 Vol 15 No 4, 2004 Vol 15 No 4, 2004 Vol 15 No 3, 2004 Vol 15 No 2, 2004 Vol 15 No 2, 2004 Vol 15 No 1, 2004 Vol 15 No 1, 2004 Vol 14 No 4, 2003 Vol 14 No 3, 2003 Vol 14 No 2, 2003 Vol 14 No 1, 2003 Vol 14 No 1, 2003 Vol 13 No 4, 2002 Vol 13 No 4, 2002 Vol 13 No 3, 2002 Vol 13 No 3, 2002 Vol 13 No 2, 2002 Vol 13 No 2, 2002 Vol 13 No 1, 2002 Vol 12 No 4, 2001 Vol 12 No 4, 2001 Vol 12 No 3, 2001 Vol 12 No 2, 2001 Vol 12 No 2, 2001 Vol 12 No 1, 2001 Vol 12 No 1, 2001 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA