Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

INDONESIAN JOURNAL OF PHARMACY

3
Website

Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

Arjuna Subject : -

Articles 10 Documents

Search results for , issue "Vol 25 No 3, 2014" : 10 Documents clear

OPTIMIZATION OF EXTRACTION CONDITIONS FOR ANDROGRAPHOLIDE USING FRACTIONAL FACTORIAL DESIGN Mohamad Rafi
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Andrographolide is a major bioctive compound found in king of bitter (Andrographis paniculata). In this study, the extraction method and its condition were investigated in order to get an extract with maximum amount of andrographolide by comparing three other extraction methods, i.e. maceration, soxhletation and ultrasonication and also determination for the optimum condition of the selected extraction method. The highest andrographolide amount was found by maceration, so this method was choosen for further optimization of extraction condition. The optimum condition based on the prediction amount from 27 factor combinations was obtained in 360 times of extraction time, 2g/100mL of sample to solvent ratio, and 3fold of extraction frequency with prediction of andrographolide amount was 3.50%. While by using prediction profile, the optimum condition was obtained in 360min of extraction time, 2 g/100mL of sample and solvent ratio, and 4 times of extraction frequency with the amount was 3.47-3.74%.

ASSESSMENT OF ANTIMICROBIAL ACTIVITY OF NOVEL DISINFECTANT BASED ON PEROXYGEN/BIGUANIDE/ALCOHOL COMBINATION Mostafa Essam Eissa
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A new disinfectant formula based on combination of Hydrogen peroxide 0.6g%, Chlorohexidine gluconate 0.5g% and Isopropanol 70.5g% was investigated to be used as broad spectrum disinfectant in an attempt to make better control over microbial bioburden in clean area during critical processes. A proper neutralization method was first implemented using combi-nation of dilution 1:10 (v/v) and chemical inactivation method using LBC3T then disinfectant efficacy study was conducted using surface challenge test and finally compatibility with other disinfectants was performed to ensure that there is no adverse interaction between them. This new product demonstrated more than 3 log reduction (LR) in less than one minute against tested vegetative bacteria and yeast Bacillus subtilis (>4.68, >4.81, >3.85 and >4.88), Pseudomonas aeruginosa (>4.00, >4.34, >3.85 and >4.04) Candida albicans (>4.11, >4.18, >4.95 and >4.48), Micrococcus lylae (>5.56, >5.56, >5.65 and 5.74) and Leifsonia aquaticum (>5.82, 5.79, >5.75 and >5.74) but about 15min were needed to achieve high log reduction against Aspergillus niger (3.02, 2.94, 2.91 and 3.10) and no remarkable log reduction of bacterial spores of Bacillus subtilis and Bacillus pumilus even after 30min of contact time on coupons inoculated with microorganisms. There is no interaction between this new formula and any other commonly used disinfectants in pharma-ceutical facility. The new disinfectant may be used as sanitizer with good activity but not as sporicidal agent for up to 30min contact time.

ANTICANCER ACTIVITY OF MANGOSTEEN PERICARP DRY EXTRACT AGAINST MCF-7 BREAST CANCER CELL LINE THROUGH ESTROGEN RECEPTOR -α Setiawati, Agustina
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Breast cancer has very complex morphological and molecular characteristic. Estrogen receptor is one of biomarker in breast cancer progression, more than 60% breast cancer overexpress estrogen receptor α (ERα). Xanthone in Garcinia mangostana was investigated whether to have anticancer activity on colorectal, prostate, lung, blood and breast cancer. This research was focused on molecular mechanism of anticancer activity of mangosteen pericarp extract (MPE) on ER-α. This study used MCF-7 cells as a model of ER-α overexpressed breast cancer cells. Cytotoxic study towards MCF-7 cells was designed by using MTT test, further apoptotic induction assay was determined by double staining method using acridine orange and ethidium bromide. Extract molecular mechanism against breast cancer was assayed by immunocytochemistry. The MTT data was analyze using probit analysis to get IC50 then apoptosis and immunocytochemistry data were analysis qualitative analysis. MPE had strong cytotoxic activity on MCF-7 cells with IC50 of 45μg/mL and morphological changes passed through apoptosis induction. The expression of ER-α in MPE treated cells was same as untreated cells. MPE did not suppress ER-α in both nucleus and cytoplasm. Anticancer activity of MPE misht be mediated by other gene involved in ER-α signaling pathway in breast cancer cells.

SUBSTANTIATION ON SHORT TERM EFFICACY AND SAFETY OF INSULIN ANALOGUES IN NORTH INDIAN SUPERSPECIALITY HOSPITAL Koladi, Mohammad Ali
INDONESIAN JOURNAL OF PHARMACY Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Diabetes mellitus is associated with high morbidity and mortality among patients and its prevalence is increasing at an alarming rate worldwide. Insulin analogues are reported to have better efficacy and safety as compared to conventional insulin therapy, however, substantiation of data in different geographical areas with genomic variation is yet to be established. The study was aimed to evaluate and compare the effectiveness and clinical safety profile of insulin analogues with regular insulin. In this prospective, randomized, observational study conducted at a Superspeciality hospital in India,78 diabetic patients on insulin therapy were recruited. The efficacy and safety markers of 24 patients on biphasic insulin analogue, 33 on recombinant insulin analogue and 21 on regular insulin were observed for 13 weeks. The collected data was statistically analyzed by using Instat software.The efficacy markers such as glycosylated hemoglobin, fasting and postprandial glucose values showed superior improvement with the insulin analogues at the end of 13 weeks study. Insulin analogues produced significantly fewer incidents of minor hypoglycemia without any significant alteration in BMI and weight gain. The results of our studies suggest that insulin analogues are safer and effective with regards to glycemic control and in the event of hypoglycemia over regular insulin.

APPLICATION OF MONTMORILLONITE, ZEOLITE AND HYDROTALCITE NANOCOMPOSITE CLAYS-DRUG AS DRUG CARRIER OF SUSTAINED RELEASE TABLET DOSAGE FORM Ainurofiq, Ahmad
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Captopril is an angiotensin converting enzyme (ACE) inhibitor asÂ antihypertensive treatment with half-life about 2h. Development of sustained-release dosage form can maintenance the drug concentration at therapeutic window in long period of time with constant release. Montmorillonite, zeolite and hydrotalcite nano-composites were used as drug carrier as sustained release dosage form. This study aimed to determine the drug release from nanocomposite of montmorillonite-drug, zeolite-drug and hydro-talcite-drug. Nanocomposite drug and carriers were made with the model drug was dispersed in carrier with matrix system. Matrices used montmorillonite, zeolite and hydrotalcite with concentrations of 20%, 30% and 40%. Characterization of matrices were done by testing the physical properties of the granules and drug release. Dissolution test using apparatus II USP model with speed rotation of 50rpm of, 900mL of HCl 0.1N as medium. The results were compared statistically with one way ANOVA 95% of interval confidence. The results showed that the difference of matrices and concentrations gave the difference effect in flow time, compact-tibility, DE360, initial burst release and maintenance release (p<0.05). Nanocomposites between drug and nanoclays occurred after 60min were shown with decreasing the drug release rate. Nanocomposite was formed with the drug molecules adsorb on nanoporous of carrier material. Increasing of clays concentration improved the fluidity and compactibility, reduced the drug release.

CONTROLLED RELEASE OF GABAPENTIN THROUGH RETICULATED CHITOSAN AND HYDROXYETHYL CELLULOSE HYDROGEL MATRIX TABLETS Narasimha S. Managoli
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The aim of the present work was to prepare hydrogel matrix tablets using crosslinked chitosan and hydroxyethyl cellulose (HEC) for controlled release of gabapentin. The chitosan was crosslinked with acetaldehyde and used for the preparation of hydrogel matrix tablets along with hydroxylethyl cellulose by wet granulation method. The samples were characterized by FTIR, DSC, TGA, XRD, SEM and evaluated drug content, swelling pattern and drug release. The matrix tablets were capable of releasing the drug for 24h depending upon the formulation variables. It was observed that as the concentration of HEC increased in the tablets, the drug release was also increased. On the other hand, as the crosslinking of chitosan was increased, the drug release was decreased. Drug release mechanism followed non-Fickian transport. This study demonstrated that the blend hydrogel matrix tablets of crosslinked chitosan and HEC could be a versatile drug delivery system for controlled release of gabapentin.

DEVELOPMENT AND CHARACTERIZATION OF POLYCLONAL ANTIBODY OF RECOMBINANT HUMAN INTERFERON Α2B IN NEW ZEALAND WHITE RABBIT Heni Rachmawati
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

We have developed recombinant wild type and mutant human interferon α2b (rhIFNα2b) from synthetic gene in Escherichia coli. To identify the successful product of the proteins, immunology-based assay was suggested due to specificity for characterization. This work was aimed to develop and characterize rhIFNα2b polyclonal antibody generated in White New Zealand rabbits. The rhIFNα2b was overproduced in Escherichia coli BL21 containing rhIFNα2b synthetic gene in pET32b.The protein was obtained as inclusion bodies, refolded, purified using nickel affinity chromatography, and characterized using polyacrylamide gel electrophoresis. The purified rhIFNα2b protein was injected into rabbits for 21 days. Absorption of E.coli antibody was done using total E. coli protein to remove antibody againts host cell. The generation of antibody was monitored using dot blot and Western blot methods and quantified using Enzyme Linked Immunsorbant Assay (ELISA). To do so, rhIFNa2b was used as an antigen. The result showed that the rhIFNα2b was produced as a His-tag protein fusion of 33kDa in size. The results of dot blot and Western blot analyses strongly indicated that antibody against rhIFNα2b was generated and specifically recognized rhIFNα2b. ELISA showed that the titer of the polyclonal anti-rhIFNα2b was 1:10.000. In conclusion, polyclonal antibody spesifically against rhIFNα2b protein was successfully detected with high titer after 21 days after rabbit immunization.

FORMULATION AND EVALUATION OF DEXTROMETHORPHAN HYDROBROMIDE CONTROLLED RELEASE HOLLOW MICROSPHERES USING NATURAL POLYMER C, Sai Sathavahana
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The objective of this study was to formulate and evaluate gastric floating dosage forms of hollow microspheres for controlled delivery of dextromethorphan hydrobromide (DBM). Hollow microspheres were prepared by emulsion polymerization method using natural polymer gelatin as release retardant and coating agent. Hollow microspheres were characterized by FT-IR, DSC, SEM, and micromeritic properties. FT-IR and DSC studies showed no chemical interaction between the drug and polymers. Hollow microspheres were evaluated for percentage yield, encapsulation efficiency and in vitro release studies. The obtained angle of repose, % Carrâ€™s index, Hausner ratio, and tapped density values were within the limits indicating good flow properties. The surface morphology revealed that microspheres were spherical with minute pores and invert dents on the surface. Among all the formulations, F-3 of hollow microspheres showed highest drug release of 79.69Â±0.12% at the end of 12h which was considered as the best formulation. The release data was fitted to various mathematical models such as, Higuchi, Korsmeyer-Peppas, First-order, and Zero order to evaluate the release kinetics and mechanism of the drug release. The release kinetics indicated that drug release followed non-fickian diffusion mechanism. Results of the stability studies showed that there were no significant changes in the drug content and physical appearance.Â

BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT Jutti Levita
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Andrographis paniculata or sambiloto is one of the most widely used medicinal herbs in Indonesia. The main bioactive chemical constituent, andrographolide, has been reported to have various pharmacological activities. Besides its function for medical purposes, the sambiloto herbs infusion is frequently taken to maintain health. This study was conducted to determine the bioavailability of sambiloto herbs infusion in rabbit plasma, stomach, and liver, calculated as total andrographolide. Fourteen male New Zealand white rabbits were used in this study. Sambiloto herbs infusion were administered orally at the dose 7.04mL/kg body weight to each rabbit. Blood samples were taken at intervals 0.0; 0.5; 1.5; 2.0; 3.0; and 5.0h after infusion administration. Sambiloto herbs infusion, which are calculated as andrographolide, levels in plasma, stomach, and liver were analyzed by high performance liquid chromatography using C-18 column as stationary phase and a mixture of methanol-double distilled water (60:40) as mobile phase. Bioavailability parameters obtained were Cmax 0.5549µg/mL (in stomach), 0.2136µg/mL (in plasma), 0.0051µg/mL (in liver); while tmax 1h (in stomach), 1.5h (in plasma), 2h (in liver); and AUC 1.7451µg.h/mL (in stomach), 0.434µg.h/mL (in plasma), 0.0038µg.h/mL (in liver). These data showed that in healthy animals, sambiloto herbs infusion was fastly absorbed from the stomach, distributed in the circulation system, and metabolized in the liver, in subsequent process. Sambiloto herbs infusion showed good bioavailability in rabbit.

IMPROVEMENT OF DISSOLUTION RATE OF INDOMETHACIN FROM FAST DISSOLVING TABLETS Parhi, Rabinarayan
INDONESIAN JOURNAL OF PHARMACY Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

In the current study Indomethacin (IM) fast dissolving tablets (FDTs) were prepared by direct compression technique in order to enhance its dissolution rate. The tablets were formulated using two different approaches; super-disintegration and efferves-cence. A combination formulation of above approaches was also developed to further improve its properties. The super-disintegrants used in the formulae were sodium starch glycolate (Primogel), cross-povidone (Kollidon) and cross-carmellose (Ac-di-sol). Sodium bicarbonate and citric acid combination was employed as effervescent ingredients. The prepared powder mixtures of IM were subjected to evaluation of various pre-compression parameters and tablets were evaluated for weight variation, dimension, hardness, friability, drug content, disintegration, wetting time, uniformity of dispersion, in vitro drug release and stability studies. The FT-IR spectra shown there are no interaction between of IM with excipient. The results of pre-compression studies indicate acceptable flow property for all the powder mixtures. The data of weight variation, dimension, hardness, friability, uniformity of dispersion and drug content studies were within the official limits. The wetting time and disintegration time decreases considerably with the increase in super-disintegrants amount. By using the combination approach, the disintegration and wetting time further decreased. In vitro dissolution studies were carried out using phosphate buffer pH 6.8 as dissolution medium for 60min and observed that formulation IF9, among super-disintegration approach, released highest percentage (97.13Â±2.09) of IM. In vitro drug release was highest (98.54Â±2.89) at 60 min for formulation IF11, when all the formulations were taken into consideration. The stability study was performed on the promised formulation IF11 at 40Â±2oC/ 75Â±5% RH for 3 months and the results indicated that there were no significant changes in aforesaid tablet properties.

Page 1 of 1 | Total Record : 10

Filter by Year

2014 2014

Filter By Issues

All Issue Vol 31 No 2, 2020 Vol 31 No 1, 2020 Vol 31 No 1, 2020 In Press Vol 30 No 4, 2019 Vol 30 No 3, 2019 Vol 30 No 2, 2019 Vol 30 No 2, 2019 Vol 30 No 1, 2019 Vol 30 No 1, 2019 Vol 29 No 4, 2018 Vol 29 No 4, 2018 Vol 29 No 3, 2018 Vol 29 No 3, 2018 Vol 29 No 2, 2018 Vol 29 No 1, 2018 Vol 28 No 4, 2017 Vol 28 No 4, 2017 Vol 28 No 3, 2017 Vol 28 No 3, 2017 Vol 28 No 2, 2017 Vol 28 No 2, 2017 Vol 28 No 1, 2017 Vol 27 No 4, 2016 Vol 27 No 4, 2016 Vol 27 No 3, 2016 Vol 27 No 3, 2016 Vol 27 No 2, 2016 Vol 27 No 2, 2016 Vol 27 No 1, 2016 Vol 27 No 1, 2016 Vol 26 No 4, 2015 Vol 26 No 4, 2015 Vol 26 No 3, 2015 Vol 26 No 3, 2015 Vol 26 No 2, 2015 Vol 26 No 1, 2015 Vol 26 No 1, 2015 Vol 25 No 4, 2014 Vol 25 No 4, 2014 Vol 25 No 3, 2014 Vol 25 No 3, 2014 Vol 25 No 2, 2014 Vol 25 No 1, 2014 Vol 25 No 1, 2014 Vol 24 No 4, 2013 Vol 24 No 4, 2013 Vol 24 No 3, 2013 Vol 24 No 3, 2013 Vol 24 No 2, 2013 Vol 24 No 2, 2013 Vol 24 No 1, 2013 Vol 24 No 1, 2013 Vol 23 No 4, 2012 Vol 23 No 3, 2012 Vol 23 No 2, 2012 Vol 23 No 2, 2012 Vol 23 No 1, 2012 Vol 23 No 1, 2012 Vol 22 No 4, 2011 Vol 22 No 4, 2011 Vol 22 No 3, 2011 Vol 22 No 3, 2011 Vol 22 No 2, 2011 Vol 22 No 2, 2011 Vol 22 No 1, 2011 Vol 21 No 4, 2010 Vol 21 No 4, 2010 Vol 21 No 3, 2010 Vol 21 No 2, 2010 Vol 21 No 2, 2010 Vol 21 No 1, 2010 Vol 21 No 1, 2010 Vol 20 No 4, 2009 Vol 20 No 4, 2009 Vol 20 No 3, 2009 Vol 20 No 3, 2009 Vol 20 No 2, 2009 Vol 20 No 1, 2009 Vol 20 No 1, 2009 Vol 19 No 4, 2008 Vol 19 No 3, 2008 Vol 19 No 3, 2008 Vol 19 No 2, 2008 Vol 19 No 1, 2008 Vol 19 No 1, 2008 Vol 18 No 4, 2007 Vol 18 No 3, 2007 Vol 18 No 3, 2007 Vol 18 No 2, 2007 Vol 18 No 1, 2007 Vol 17 No 4, 2006 Vol 17 No 3, 2006 Vol 17 No 3, 2006 Vol 17 No 2, 2006 Vol 17 No 2, 2006 Vol 17 No 1, 2006 Vol 17 No 1, 2006 Vol 16 No 4, 2005 Vol 16 No 4, 2005 Vol 16 No 3, 2005 Vol 16 No 2, 2005 Vol 16 No 2, 2005 Vol 16 No 1, 2005 Vol 16 No 1, 2005 Vol 15 No 4, 2004 Vol 15 No 4, 2004 Vol 15 No 3, 2004 Vol 15 No 2, 2004 Vol 15 No 2, 2004 Vol 15 No 1, 2004 Vol 15 No 1, 2004 Vol 14 No 4, 2003 Vol 14 No 3, 2003 Vol 14 No 2, 2003 Vol 14 No 1, 2003 Vol 14 No 1, 2003 Vol 13 No 4, 2002 Vol 13 No 4, 2002 Vol 13 No 3, 2002 Vol 13 No 3, 2002 Vol 13 No 2, 2002 Vol 13 No 2, 2002 Vol 13 No 1, 2002 Vol 12 No 4, 2001 Vol 12 No 4, 2001 Vol 12 No 3, 2001 Vol 12 No 2, 2001 Vol 12 No 2, 2001 Vol 12 No 1, 2001 Vol 12 No 1, 2001 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA