Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

0.659

P-Index

This Author published in this journals

All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Cancer Chemoprevention INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas (JFSK) Indonesian Journal of Chemistry International Journal of Community Service Learning JFIOnline

Agustina Setiawati

Faculty Of Pharmacy, Universitas Sanata Dharma, Yogyakarta

Author-ID : 377205

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Veterinary

Published : 11 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual Setiawati, Agustina
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 3 (2015)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Resistensi antibiotik masih menjadi perhatian dalam pengobatan penyakit infeksi. Penggunaan antibiotik yang tidak sesuai meningkatkan kasus terjadinya resistensi antibiotik. Penelitian ini didesain untuk membuat metode yang dapat digunakan untuk membuat kultur Staphycoccus aureus (S.aureus) yang resisten terhadap amoxicillin. Selanjutnya, bakteri tersebut dapat digunakan sebagai model bakteri resisten adaptif dalam penelitian mikrobiologi. Penelitian ini menggunakan metode adaptif gradual dengan sub kultur bakteri pada media mengandung amoxicillin sub-MIC. Konsentrasi sub-MIC ditingkatkan setiap minggu selama tiga minggu. MIC amoxicillin diuji kembali setiap akhir sub kultur sub-MIC pada satu konsentrasi. Amoxicillin bersifat bakterisidal terhadap S.aureus dengan MIC 0,25µg/mL. Konsentrasi amoxicillin sub MIC yang digunakan adalah 0,10; 0,15 dan 0,20 µg/mL. Metode ini berhasil membuat S.aureus resisten dengan meningkatkan MIC 300x pada akhir sub kultur 0,10 µg/mL dan meningkatkan MIC 400x pada akhir sub kultur 0,15 µg/mL MIC tidak meningkat. Metode adaptif gradual berhasil meningkatkan resistensi S.aureus terhadap amoxicillin.

Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual Setiawati, Agustina
Jurnal Farmasi Indonesia Vol 7, No 3 (2015)
Publisher : Jurnal Farmasi Indonesia

Resistensi antibiotik masih menjadi perhatian dalam pengobatan penyakit infeksi. Penggunaan antibiotik yang tidak sesuai meningkatkan kasus terjadinya resistensi antibiotik. Penelitian ini didesain untuk membuat metode yang dapat digunakan untuk membuat kultur Staphycoccus aureus (S.aureus) yang resisten terhadap amoxicillin. Selanjutnya, bakteri tersebut dapat digunakan sebagai model bakteri resisten adaptif dalam penelitian mikrobiologi. Penelitian ini menggunakan metode adaptif gradual dengan sub kultur bakteri pada media mengandung amoxicillin sub-MIC. Konsentrasi sub-MIC ditingkatkan setiap minggu selama tiga minggu. MIC amoxicillin diuji kembali setiap akhir sub kultur sub-MIC pada satu konsentrasi. Amoxicillin bersifat bakterisidal terhadap S.aureus dengan MIC 0,25Âµg/mL. Konsentrasi amoxicillin sub MIC yang digunakan adalah 0,10; 0,15 dan 0,20 Âµg/mL. Metode ini berhasil membuat S.aureus resisten dengan meningkatkan MIC 300x pada akhir sub kultur 0,10 Âµg/mL Â dan meningkatkan MIC 400x pada akhir sub kultur 0,15 Âµg/mL MIC tidak meningkat. Metode adaptif gradual berhasil meningkatkan resistensi S.aureus terhadap amoxicillin.

Cytotoxic of Ganoderma lucidum in Colon Cancer through Cyclooxygenase 2 (COX-2) as Its Molecular Target Setiawati, Agustina
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.14

Many studies were designed explore chemopreventive activity of natural products on colon cancer especially addressing COX-2 as molecular target. Another promising source of natural product that potentially exhibit anticancer activity on colon cancer is Ganoderma lucidum. This study assessed selectivity of cytotoxic effect of G. lucidum extract on WiDr to Vero cells and investigated molecular mechanism on COX-2. G. lucidum ex-tract was prepared by reflux extraction method; in vitro anticancer was assayed by MTT method on WiDr and Vero cell line. This study applied apoptosis induction assay to observe cell death mechanism using double staining method; further COX-2 expression was stained by immunocytochemistry method. G. lucidum extract has cytotoxic effect on WiDr cells with IC50 135 Âµg/mL. However, the cytotoxic effect had low selectivity to-wards Vero cells with Selectivity Index (SI) 3.66. The extract induced apoptosis and suppressed COX-2 ex-pression in WiDr cells. G. lucidum extract was potential to be developed as anticancer agent towards colon cancer.

ANTICANCER ACTIVITY OF MANGOSTEEN PERICARP DRY EXTRACT AGAINST MCF-7 BREAST CANCER CELL LINE THROUGH ESTROGEN RECEPTOR -α Setiawati, Agustina
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Breast cancer has very complex morphological and molecular characteristic. Estrogen receptor is one of biomarker in breast cancer progression, more than 60% breast cancer overexpress estrogen receptor α (ERα). Xanthone in Garcinia mangostana was investigated whether to have anticancer activity on colorectal, prostate, lung, blood and breast cancer. This research was focused on molecular mechanism of anticancer activity of mangosteen pericarp extract (MPE) on ER-α. This study used MCF-7 cells as a model of ER-α overexpressed breast cancer cells. Cytotoxic study towards MCF-7 cells was designed by using MTT test, further apoptotic induction assay was determined by double staining method using acridine orange and ethidium bromide. Extract molecular mechanism against breast cancer was assayed by immunocytochemistry. The MTT data was analyze using probit analysis to get IC50 then apoptosis and immunocytochemistry data were analysis qualitative analysis. MPE had strong cytotoxic activity on MCF-7 cells with IC50 of 45μg/mL and morphological changes passed through apoptosis induction. The expression of ER-α in MPE treated cells was same as untreated cells. MPE did not suppress ER-α in both nucleus and cytoplasm. Anticancer activity of MPE misht be mediated by other gene involved in ER-α signaling pathway in breast cancer cells.

SUATU KAJIAN MOLEKULER KETERGANTUNGAN NIKOTIN Agustina Setiawati
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 10, No 2 (2013)
Publisher : Sanata Dharma University

Abstract: Nicotine, a simple chemical substance from tobacco, stimulates addiction byneuroadaptation inducing rewards system in human brain. Molecular mechanism of nicotineaddiction in rewards system are mediated by nicotinic acetylcholine receptor (nAChR) andactivate dopaminergic neuron. Thus, nicotine addiction are also induce other neurottransmitterand hormone release: norephinefrin, serotonin, opioid, glutamat and mono amine oxidase.Keywords: nicotine, addiction, rewards system.

ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI Agustina Setiawati; Sri Hartati Yuliani; Enade Perdana Istyastono; Michael Raharja Gani; Evy Fenny Veronica; Dina Christin Ayuning Putri; Reza Eka Putra; David Chandra Putra; Agnes Mutiara Kurniawan
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 11, No 1 (2014)
Publisher : Sanata Dharma University

Rapid and simple quantitative analysis isoflavones tempe using densitometric TLChas been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distanceof 1 ?L sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linearrelationship obtained at 0.08 - 2 ?g/spot with r= 0.9986. The LOD and LOQ of isoflavone were0.014 ?g/spot and 0.048 ?g/spot. Genistein contained in tempe was 0.151 0.005 % b/b.

CYTOTOXIC ACTIVITY OF Anredera cordifolia LEAF EXTRACT ON HELA CELLS Dita Maria Virginia; Roni Permana Saputra; Agustina Setiawati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 1 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.1.863

Cervical cancer is the second most frequently occurring cancer among females all over the world. Since the best strategy against cancer is to kill the cancer cells without endangering the normal cells, discovering a potentially selective anticancer agent from plants has become very challenging for researchers worldwide. A previous study on Anredera cordifolia, known as binahong in Indonesia, revealed its cytotoxic activity on HeLa cervical cancer cells with IC50 75 Âµg/mL. However, the selectivity of the chemical agent and its molecular target was not investigated. Thus, this study was aimed at determining the selectivity of ethanolic extract of Anredera cordifolia leaf (EAC) on Vero cells and its molecular target on HeLa cells. The extracts were prepared by macerating A. cordifolia leaf powder in 70% ethanol. The viability of Vero cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. While the cell cycle of HeLa cells was analyzed using the flow cytometry, the molecular target of the extract was investigated by using the immunocytochemical staining. The results exhibited the selective cytotoxicity of EAC on HeLa cells compared to Vero cells with a Selectivity Index (SI) of 17.36. It arrested the G1/S phase of the cell cycle and suppressed the Bclâ€“2 expression, the anti-apoptotic protein, which also regulates the cell cycle. These findings confirmed the use of A. cordifolia leaf extract as a promising anticancer agent against cervical cancer, particularly the HeLa cells.

The Doxorubicin-Induced G2/M Arrest in Breast Cancer Cells Modulated by Natural Compounds Naringenin and Hesperidin Sendy Junedi; Adam Hermawan; Aditya Fitriasari; Agustina Setiawati; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 12, No 2 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss2pp83-89

Doxorubicin as the common drug for breast cancer has been widely proposed to use in combine with a natural compound in order to overcome its side effects such as cardiotoxicity and resistance. Previously, we reported that naringenin and hesperidin, the abundant flavanons in citrus fruit peel, increased cytotoxic and apoptosis activities of doxorubicin in doxorubicin resistant breast cancer cells (T47D and MCF-7 cells). Since doxorubicin arrests G2/M phase in most cancer cells, both flavanons are speculated to affect the similar phase in breast cancer cells. Cell cycle distributions were determined by flowcytometry using propidium iodide (PI) to stain DNA of the cells. Combination of naringenin or hesperidin with doxorubicin increased accumulation of T47D cells in G2/M phase, while in MCF-7 cells, accumulated cells in G2/M phase were decreased, accompanying with slightly increased in G1 phase. Naringenin itself had no effect on cell cycle of both cells. Whereas, hesperidin arrested G2/M and G1 phases in T47D and MCF-7 cells, respectively. The different effect of naringenin and hesperidin in T47D and MCF-7 cells is most likely caused by difference of p53 status. In p53 mutant, T47D cells, naringenin and hesperidin supported mechanism of doxorubicin to arrest at G2/M that to be considered via p53-independent pathway. Whereas, in p53 wild-type MCF-7 cells, naringenin and hesperidin decreased G2/M arrest, suggesting that both flavanons do not utilize cell cycle arrest for their anticancer activity with doxorucibin. This study revealed that potential co-chemoterapeutic agents, naringenin and hesperidin distinctly modulated cell cycle arrest induced by doxorubicin according to the characteristic of breast cancer cells.Keywords: naringenin, hesperidin, doxorubicin, cell cycle, breast cancer cells.

Retrospective Validation of a Structure-Based Virtual Screening Protocol to Identify Ligands for Estrogen Receptor Alpha and Its Application to Identify the Alpha-Mangostin Binding Pose Agustina Setiawati; Florentinus Dika Octa Riswanto; Sri Hartati Yuliani; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 14, No 2 (2014)
Publisher : Universitas Gadjah Mada

The publicly available enhanced data of ligands and decoys for estrogen receptor alpha (ERα) which were recently published has made the retrospective validation of a structure-based virtual screening (SBVS) protocol to identify ligands for ERα possible. In this article, we present the retrospective validation of an SBVS protocol using PLANTS molecular docking software version 1.2 (PLANTS1.2) as the backbone software. The protocol shows better enrichment factor at 1% false positives (EF1%) value and the Area Under Curve (AUC) value of the Receiver Operator Characteristic (ROC) compared to the original published protocol. Moreover, in all 1000 iterative attempts the protocol could reproduce the co-crystal pose of 4-hydroxitamoxifen in ERα binding pocket. It shows that the protocol is not only able to identify potent ligands for ERα but also able to be employed in examining binding pose of known ligand. Thence, the protocol was successfully employed to examine the binding poses of α-mangostin, an ERα ligand found in the Garcinia mangostana, L. pericarp.

EFEK SITOTOKSIK EKSTRAK DIETIL ETER HERBA PEGAGAN EMBUN (Hydrocotyle sibthorpioides Lmk.) TERHADAP SEL KANKER PAYUDARA MCF-7 Agustina Setiawati; Yohanes Dwiatmaka
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 12, No 1 (2015)
Publisher : Sanata Dharma University

Abstract: Triterpenoid saponin, hydrocosaponin A-F, isolated compound from Hydrocotyle sibthorpioides Lmk. performed cytotoxic activity on KB, Daoy and WiDr cancer cells. Methanolic extract of this herb was also investigated in inhibiting cancer development on liver and cervical cancer. Therefore, it is potential to be developed as chemopreventive agent. The objective of this study is to determine cytotoxic activity and compound in diethyl ether extract of Hydrocotyle sibthorpioides Lmk. herbs. The cytotoxic effect of extract on MCF-7 breast cancer cell line was assayed using MTT method while phytochemical analysis was observed using normal phase Thin Layer Chromatoraphy (TLC). Diethyl ether extract of Hydrocotyle sibthorpioides had cytotoxic effect on MCF-7 cells with IC50 365 g/mL. TLC analysis observed that the extract contain flavonoid compounds. Keywords : Hydrocotyle sibthorpioides, MCF-7, cytotoxic, flavonoid

Co-Authors Adam Hermawan Aditya Fitriasari Agnes Mutiara Kurniawan David Chandra Putra Dina Christin Ayuning Putri Dita Maria Virginia Edy Meiyanto Enade Perdana Istyastono Enade Perdana Istyastono Evy Fenny Veronica Fizkha Hanindita Flavia Domitilla Erika Setyajati Florentinus Dika Octa Riswanto Michael Raharja Gani Nyoman Bayu Wisnu Kencana Ratna Asmah Susidarti reza eka putra Roni Permana Saputra Sendy Junedi Sri Hartati Yuliani Yohanes Dwiatmaka

Title

Found 11 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 11 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 11 Documents
Search