cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol. 10 No. 2 (2020)" : 10 Documents clear
First record of the genus Metacirolana sp. and Cymodoce tribullis (Crustacea; Isopoda) from South Andaman, Andaman Islands, India Pathan Anil; Ameen Ummath; Bijin Mohammed PS; Kaderambil Arjunan Jayaraj
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.08

Abstract

Isopoda are one of the most poorly known crustaceans in Andaman and Nicobar Islands. The present study has recorded genus Metacirolana sp. and Cymodoce tribullis, for the first time from South Andaman. This recent sighting provides a new distribution of this two species in the Indian province. The specimens were collected from the seaweeds of intertidal regions of Kodiyaghat, South Andaman, Andaman Islands. The morphological characteristics of these two species were described. Furthermore, this study adds two more species to the isopoda check list of Andaman and Nicobar Islands. The present study also provides additional information on epifaunal communities associated with the seaweeds in the intertidal region of Kodiyaghat, South Andaman.
Assessment of Biological Spectrum and Phenological Behaviours of Coastal Vegetation in East Midnapore District, Eastern India Uday Kumar Sen; Ram Kumar Bhakat
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.05

Abstract

The open coastlines of the world are dominated by sand dunes and salt marshes. They are the primary areas for human recreation and provide a wide range of frequently overlooked ecosystem services. Their intrinsic ecological significance lies in having unique biological assemblies and supporting a range of ecological processes, such as water filtration and nutrient cycling. In addition, in the coastal zone, these areas link marine and terrestrial systems. The objective of this paper is to check the psammophytic species diversity, conservation status and threat; and also prepare a biological spectrum m in the Costal East Midnapore (CEM) district's sand dune and salt marsh areas. A part of floristic inventories in the CEM, various field trips in different season were carried out, quadrate method is done to examine the ecological impact and richness. Specimens were collected, prepared voucher specimen and enlisted according APG IV classification system to prepare biological spectrum. CEM vegetation was diverse and composed of 162 species of 132 genera distributed among 52 families of 28 orders according to APG IV classification, of which 37 species were exotics. Fabales (19.14%) and Fabaceae (19.14%) were the dominant order and family. Biological spectrum shows the study area was classified as “thero-chamaephytic” type of phytoclimate. Mesophyll (24.07%) and lanceolate as well as oblong (19.14%) were found to be high in the leaf size spectrum and lamina. The area of study was highly ecotonal potential and fairly undisturbed. This study is useful in comparing and contrasting the adjacent natural vegetation along the gradients of the environment; and recommends effective long-term conservation and better management of the coastal area.
A Comparative Genomics Pipeline for In Silico Characterization and Functional Annotation of Short Hypothetical Proteins Soumyajit Guha; Shuvam Das; Sayak Ganguli
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.06

Abstract

Hypothetical proteins are the proteins whose existence has been anticipated, but for which there are certain scarcities of experimental evidences about its structure, function or linkage to any known genes. Sequencing of several genomes has resulted in numerous predicted open reading frames to which structure or function(s) cannot be readily assigned and sometimes they can make up a significant portion of a genome. In this study, we designed a pipeline for the study and efficient functional annotation of short hypothetical proteins (only which were < 400 amino acids) comparing two case studies, using amino acid sequence informations retrieved from two different protein databases. The investigation and in-silico analysis of likely functional aspects of hypothetical proteins were performed employing various computational methods and tools based on sequence similarity, identification of targeting signals, presence of known protein domains, physicochemical characterization, etc. Our annotation pipeline was able to annotate 90 hypothetical proteins out of 100 compared to evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) databases' annotation of 82 proteins, which is about 8% more compared to eggNOG for case study 1 and 78 hypothetical proteins out of 96 compared to eggNOG’s annotation of 58 proteins, which is about 20.83% more compared to eggNOG for case study 2. It was also seen that some hypothetical proteins had a high aliphatic index, indicating higher thermostability in extreme environments. From this study subcellular localization involving cytoplasmic proteins and membrane proteins were also predicted with higher accuracies than other proteins. Hypothetical proteins can provide an insight of different unknown structures and functions of proteins and can be an important area for further research.
A Review of Pharmaceutical Recombinant Proteins and Gene Transformation Approaches in Transgenic Poultry Sepideh Fallahi; Reza Mohammadhassan
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.09

Abstract

Transgenic animals are employed to synthesize pharmaceutical recombinant proteins for three decades.  There are many problems to use farm mammalian animals for recombinant production such as high cost of production and maintenance, long generation interval, toxic effects of some human-source pharmaceutical proteins in other mammalian and incompatibility of human-source glycosylation with the other mammalian' glycosylation that all lead to low efficiency. Thus, transgenic poultry has been considered as the bioreactor of recombinant protein production. Increasing demand for pharmaceutical human proteins caused to make considerable efforts to develop transgenic poultry producing eggs contain recombinant protein. In the present review, at the first, transgenic animal and poultry are compared for their benefits and limitations. Then, the protein content of the egg, the features, gene and promoter of the egg are studied. After that, the recent achievements of the producing pharmaceutical recombinant proteins are considered. In the following, there are some explanations about gene transformation approaches in poultry, including sperm-, testis-, PGCs, and blastocyst-mediated methods depending on CRISPR/Cas9, Retroviral vectors, and DNA microinjection techniques, and embryonic manipulation approaches such as windowing and Ex ovo for introducing and injecting transformed cells into eggs.
Antibacterial Screening of Mangrove Extract Library Showed Potential Activity against Escherichia coli and Staphylococcus aureus Kholis Abdurachim Audah; Razethy Batubara; Julkipli Julkipli; Elza Wijaya; Evi Kurniawaty; Irmanida Batubara
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.03

Abstract

Humans are at a continuous battle against different types of diseases, so that extraordinary effort to accelerate drug discovery has become a necessity. Indonesian biodiversity is abundant natural resources that can be utilized as potential drug sources. Mangroves are among potential plant medicines that grow nearly at all Indonesian coastlines. The aim of this study was to evaluate the potential of mangrove extracts (extract library) as antibacterial agents. In this study, eight mangroves species with 16 samples from different parts of the plants such as leaf, bark or root were collected. Four types of solvents with different polarity, namely water, ethanol, ethyl acetate and hexane were used in maceration of samples producing 64 extracts. Disk diffusion method was used for antibacterial screening using five bacterial strains. There were 37 extracts showed antibacterial potential with the lowest and the highest inhibition indexes were 0.0283 and 1.8983, respectively. The highest inhibition index was recorded for ethyl acetate extract of Bruguiera gymnorrhiza root (BgR (Ea)) against Escherichia coli. The second highest inhibition index was 0.7867 recorded water extract of Avicennia marina leaf (AmL (A)) against Staphylococcus aureus. Almost all of extract showed saponin and tannin in considerable amount. This supported the data that mangrove extracts were potential as antibacterial agents.
Species Richness and Endemism of Zingiberaceae in Cinchona Forest Reserve, Lantapan, Bukidnon, Philippines John Austin Lennox Faro Jayme; Noe Polo Mendez; Rainear Auxtero Mendez; Daniel F Somera; Alma B Mohagan
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.10

Abstract

This study was carried out to provide information on species richness and endemism of Zingiberaceae in Cinchona Forest Reserve, Kaatuan, Lantapan, Bukidnon, Philippines. Transect walks, opportunistic sampling and collection within the sampling quadrats were conducted along established forest trails to collect ginger species. A total of 11 species of Zingiberaceae were documented belonging to two subfamilies (Alpinioideae and Zingiberoideae) and three tribes (Alpinieae, Hedychieae, and Zingibereae). The species recorded include Adelmeria alpina Elmer, Alpinia haenkei C.Presl, A. rufa C.Presl, Etlingera fimbriobracteata (K.Schum.) R.M.Sm., E. pubimarginata (Elmer) A.D.Poulsen, Hedychium philippinense K.Schum., Hornstedtia conoidea Ridl., H. lophophora Ridl., Meistera muricarpa (Elmer) Škorničk. & M.F.Newman, Zingiber banahaoense Mood & Theilade, and Zingiber sp. Of these, H. philippinense is the only threatened species recorded. All species are endemic to the Philippines except for E. fimbriobracteata which is native to Borneo and Zingiber sp. which is unidentified to the species level. These species represent 41% of the total genera and 9% of the total species of Zingiberaceae in the Philippines. The high endemicity (82%) in the total collected species in this study and the presence of a threatened species in this area calls for protection and conservation by the stakeholders.
Growth and Development of Tristaniopsis merguensis Seedling Inoculated by Natural Ectomycorrhiza Triadiati Triadiati; Anastastia Raditya Hidayanti; Nampiah Sukarno
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.01

Abstract

Tristaniopsis merguensis (pelawan tree) is a potential plant, however, it has not cultivated and conserved optimally due to slow growth and lack of information about the cultivation. The existence of T. merguensis in the forest is important because pelawan fungi grow under the tree, particularly in the above of the roots. Information about how to grow the T. merguensis seedling is very limited; therefore, research in growth and development of T. merguensis seedling was important. The aims of this study were to determine the appropriate planting medium for T. merguensis seed germination and to stimulate growth and development of T. merguensis seedling by using its fungi. Status of T. merguensis fungi was studied by analyzing root morphology. Fungi isolation was carried out from colonized root and fungi fruit body. The stimulated the growth of T. merguensis seedling was done by using fungi isolated from colonization root in laboratory scale, as well as treated at a different phosphate concentration. The result showed that T. merguensis was associated with ectomycorrhizal fungi. Planting medium consists of sawdust and T. merguensis fine root resulted the highest percentage of germination. Seedlings were treated with ectomycorrhizal fungi grew better than without ectomycorrhizal fungi. In addition, seedlings treatment with ectomycorrhizal fungi and phosphate 25% showed the highest growth rate. 
Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) Afifah Nur Aini; Rodiyati Azrianingsih; Irfan Mustafa
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.02

Abstract

Tuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their potency to cause rotting on porang tubers. Porang tubers were immersed in bacterial suspension and incubated for 14 days. The physical properties of the tubers such as texture and color, and the chemical properties such as reducing sugar and ethanol content were determined at the end of incubation period. All infected tubers were soft, slimy and darker. Two isolates, T4 and T9A, had the highest potency to cause tuber rot indicated by the significantly higher reducing sugar content (14.38 and 15.32 mg/ml) and higher ethanol content (32.17 and 35.05%) compared to control tubers which were not infected with bacteria. Both isolates were identified as Bacillus altitudinis and Pseudomonas stutzeri, respectively, based on 16S rDNA sequence analysis.
Evaluation of the Quality of Coastal Ecosystems in the Spermonde Archipelago Using Macroalgae as Indicators Rispah Hamzah; Luchman Hakim; Catur Retnaningdyah
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.04

Abstract

The quality of water in Spermonde Archipelago, South Sulawesi has decreased as a result of high human activities and land use, this can have an impact on the life of marine biota. Macroalgae is suitable for bioindicators because they live in a sessile manner, can accumulate metals and distributed widely. This research aimed to determine coastal ecosystem quality by using macroalgae as indicators on five islands that had different anthropogenic stresses. The research was carried out in April 2019 on Barrang Caddi, Bonebatang, Barrang Lompo, Kodingareng Keke, and Badi Islands. Community structures observed included taxa richness, diversity, evenness, dominance of macroalgae and physicochemical parameters (temperature, turbidity, pH, salinity, DO, BOD, H2S, oil, grease, total Phosphate, and nitrate) as well as the measurement of the ecological condition using Quality of Rocky Bottoms (CFR) index. The results showed that physicochemical parameters (temperature, turbidity, pH, salinity, DO, H2S, oil, grease, total Phosphate) of the water still met the quality standards of sea water based on Decree of the State Minister for Environment No. 51/2004 for marine biota and other standards for macroalgae growth. BOD and nitrate have exceeded sea water quality standards for marine biota (20 mg/L for BOD and 0.008 mg/L for nitrates). A total of nine species of macroalgae were found in Barrang Caddi and Badi Island, three species in Bonebatang, and five species in Kodingareng Keke and Barrang Lompo Island. The result of Principal Component Analysis (PCA) and Biplot showed that physicochemical water quality has an influence on the macroalgae community structure. While the CFR index shows Barrang Caddi and Badi Island have poor ecological conditions and Bonebatang, Barrang Lompo, and Kodingareng Keke have bad ecological condition.
Application of Atmospheric Pressure Argon Plasma Jet (APAPJ) in Biomedical Science and Engineering Jyoti Prakash Gurung; Deepak Prasad Subedi; Rajendra Shrestha; Bhupal Govinda Shrestha
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.07

Abstract

In this paper, we demonstrated the application of atmospheric pressure argon plasma jet (APAPJ) in biomedical science such as cancer therapy and biomedical engineering such as surface modification of polystyrene Petri dishes for animal cell culture. We observed that APAPJ, when exposed to breast cancer cell line (MDA-MB-231) for 60 seconds at a distance of ~ 2.5 cm, reduced the cancer cells by 57%. This reduction in cancer cell concentration demonstrated the cancer-killing property of APAPJ. Further optimization of APAPJ's various operational parameters and in-depth biochemical study is required to figure out the mechanism responsible for killing cancer cells. Also, we investigated cell adhesion and proliferation due to APAPJ treatment by Crystal Violet (CV) staining and MTT Assay. We observed that APAPJ treatment of polystyrene Petri dishes for 60 - 240 s at a distance of ~ 3.5 cm exhibited enhancement in adhesion of breast cancer cell line (MDA-MB-231). We also demonstrated the increase in hydrophilicity by a reduction in contact angle to 30.3°±3 after 240 seconds of APAPJ treatment of polystyrene samples. The hydrophilicity of the substrate has proven to enhance cell adhesion. Consequently, APAPJ could be employed as a simple, inexpensive and environmentally friendly method for surface modification of polystyrene plates for animal cell culture.

Page 1 of 1 | Total Record : 10

 1 

Filter by Year

2020 2020


Reset
Filter By Issues
All Issue Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol 11, No 3 (2021) Vol. 11 No. 3 (2021) Vol 11, No 2 (2021) Vol. 11 No. 2 (2021) Vol. 11 No. 1 (2021) Vol 11, No 1 (2021) Vol. 10 No. 3 (2020) Vol 10, No 3 (2020) Vol. 10 No. 2 (2020) Vol 10, No 2 (2020) Vol 10, No 1 (2020) Vol. 10 No. 1 (2020) Vol 9, No 3 (2019) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol 9, No 2 (2019) Vol. 9 No. 1 (2019) Vol 9, No 1 (2019) Vol 8, No 3 (2018) Vol. 8 No. 3 (2018) Vol 8, No 2 (2018) Vol. 8 No. 2 (2018) Vol 8, No 1 (2018) Vol. 8 No. 1 (2018) Vol 7, No 3 (2017) Vol. 7 No. 3 (2017) Vol 7, No 2 (2017) Vol. 7 No. 2 (2017) Vol. 7 No. 1 (2017) Vol 7, No 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol 6, No 2 (2016) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol 6, No 1 (2016) Vol 5, No 3 (2015) Vol. 5 No. 3 (2015) Vol. 5 No. 2 (2015) Vol 5, No 2 (2015) Vol. 5 No. 1 (2015) Vol 5, No 1 (2015) Vol 4, No 3 (2014) Vol. 4 No. 3 (2014) Vol. 4 No. 2 (2014) Vol 4, No 2 (2014) Vol 4, No 1 (2014) Vol. 4 No. 1 (2014) Vol 3, No 3 (2013) Vol. 3 No. 3 (2013) Vol. 3 No. 2 (2013) Vol 3, No 2 (2013) Vol 3, No 1 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol. 2 No. 2 (2012) Vol 2, No 2 (2012) Vol 2, No 1 (2012) Vol. 2 No. 1 (2012) Vol 1, No 2 (2011) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2010) Vol 1, No 1 (2010) More Issue