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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol. 5 No. 2 (2015)" : 9 Documents clear
Partial Characterization of Salmonella gallinarum Clinical Isolate and Expression of Its Antigenic Outer Membrane Protein C (ompC) Gene in planta Ee Leen Pang; Hwei-San Loh
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.02

Abstract

Instigating approximately 100% of mortality and morbidity rates, fowl typhoids epidemiology and disease intervention have been extensively studied since 1950s. Even up-to-date, outbreaks are incessantly haunting poultry industries of major continents. Salmonella gallinarum, the etiologic agent of fowl typhoid, was used to develop a series of vaccination regime. However, treatments are gradually losing effectiveness due to residual virulence from mutated strains and rapid evolution of multi-drug resistance isolates. Hence, in planta subunit vaccine production is proposed to surpass current limitations. The homotrimeric osmoporin (Outer Membrane Protein C) is a potent candidate antigen that confers momentous stimulation of humoral and cell-mediated immune responses in broilers. This research signified the potential development of plant-expressed ompC immunogen, with the scope embarked on identification of S. gallinarum clinical isolate; construction of expression cassette; generation of recombinant vector and delivery of constructs into Nicotiana benthamiana for transient expression via agroinfiltration. The ompC transcripts and proteins were detected successfully at the molecular weights of 1.002 kbp and ~35 kDa, respectively. These preliminary findings pave the feasibility of biomanufacturing a safe and cost-effective fowl typhoid vaccine that would confer multi-protection against other significant Salmonella infections attributed to high sequence homology of ompC gene. Speed improvement is demonstrated and transient expression appears to outperform conventional platforms in expediting vaccine production for an emerging pandemic strain.
Saras Cranes in Palwal District in Southern Haryana Are Asking For Immediate Attention for Their Last Rescue Efforts Tirshem Kumar Kaushik; Rohtash Chand Gupta; Parveen Kumar Vats
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.04

Abstract

Saras Cranes Grus antigone are endangered birds of open wetlands with highly worrying depletion trends being witnessed related with disappearance of marshy and shallow perennial, expansive wetlands throughout northern India. Alongside, massive hunting in 18th, 19th and 20th centuries and even today is another serious cause for their worrisome deterioration. Also, destruction of nests, eggs, fledglings and adults by aboriginals indeliberately or deliberately is causing these cranes to perish sooner than latter, completely.Now, Saras Cranes are found in limited number and domain as four populations in the entire world including India, China, Burma, South East Asia and northern Australia. In so far as Indian Saras Crane population is concerned, it is pitiably restricted to only few hundred in all, in Etawa, Mainpuri districts of Uttar Pradesh, Kanha National Park near Bharatpur in Rajasthan, some parts of Gujarat in the west and Assam in the east.It is interesting to note that few pairs have been seen in Faridabad and Palwal districts in southern Haryana, India. These need to be protected and conserved
Anti-Lipid Peroxidation Activities of Three Selected Fruits Juices Against Cadmium Induced Liver Damage In Vitro Eko Suhartono; June Astri Nijka; Vini Yulia Anhar; Rizki Amalia Sari; Edyson Edyson; Dona Marisa
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.03

Abstract

Cadmium (Cd) is one of the most toxic heavy metal. Numerous tissues both in vitro and in vivo suggest that Cd induced lipid peroxidation. The presence of antioxidant can inhbit this process. This present study was designed to determine the effects of mango, papaya, and banana juices on on malondi aldehyde levels (MDA, product of cellular lipid peroxidation) during Cd exposure in vitro. Significant differences was observed between three selected fruits juices and MDA levels and also between concentration of fruits juices and MDA levels. The highest correlation was found between banana juices and MDA levels with IC50 1,12, and followed by papaya and mango juices with IC 50 1,37 and 3,87 respectively. The findings of this study suggest that the three selected fruits juices may exert its protective action against Cd-induced lipid peroxidation in liver homogenate in vitro possibly through its antioxidant mechanism.Key WordCadmium, Lipid Peroxidation, Antioxidant Activity and Fruits Juices
Intraperitoneal Injection of High Tumor Necrosis Factor (TNF-α) Serum Increase Soluble Fms-like Tyrosine Kinase-1 (sFlt-1) and Blood Pressure of Pregnant Mice Bramantyo Aji Wicaksono; Siti Candra Windu Baktiyani; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.01

Abstract

Preeclampsia has major symptoms of hypertension and proteinuria and is a cause of significant maternal and infant mortality in the world. The slow development of preeclampsia research possibility caused by the difficulty in acquiring animal preeclampsia. Many existing animal model have been developed, but most of them are expensive to do. The purpose of this study was to determine the effects of intraperitoneal injection of pregnant patients serum with high TNF-α levels toward sFlt-1 serum concentration and blood pressure of pregnant mice. Pregnant patients serum with high TNF-α levels (>20 pg/mL) was injected intraperitoneally to pregnant mice at gestational age 13 and 14 days. At 18 days of gestation, the blood pressure was measured, then the mice were dissected and the serum was taken to measure serum sFlt-1 concentration using ELISA kit(Bioassay Technology Laboratory, E0611Mo). The results showed there was a significant increase in blood pressure (p = 0.000) and the sFlt-1 levels (p = 0.002) of pregnant mice group that injected by pregnant patient serum with high TNF-α levels compare to control group. From these data it can be concluded that intraperitoneal injection of pregnant patients serum with high TNF-α levels to pregnant mice can increase blood pressure and sFlt-1 serum concentration of mice.
Oxidative Stress on Buccal Mucosa Wound in Rats and Rule of Topical Application of Ethanolic Extracts of Mauli Banana (Musa acuminata) Stem Wenda Fitriati Noor; Novie Aprianti; Saldy Rizky Saputra; Maharani Laillyza Apriasari; Eko Suhartono
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.05

Abstract

The present study was undertaken to evaluate the effect of a topical application of ethanolic extracts of Mauli banana stem on buccal mucosa wounds in rats (Rattus novergicus). Three sets of experiments with 3 groups male rats each consisted of 9 animals were used for studying wound oxidative stress status. Group 1 (P0) as the negative control was left to heal spontaneously; group 2 (P1) as the postive control were treated with alocair topically; and group 3 (P2) as treatment group were treated with ethanloic extracts of Mauli banana stem topically, respectively, 24h after wound creation for 3 days. The oxidative stress status was evaluated by monitoring the SOD, CAT activity, MDA and CC levels. The effect of ethanolic extracts of Mauli banana stem on oxidative stress status revealed significant increased in SOD activity, decreased in MDA levels, and no significant change both in CAT activity and CC levels compared to negative control. These results showed that The ethanolic extracts of Mauli banana stem might affect the oxidative stress status during wound healing process. Key Word : Antioxidant, Free Radicals, Mauli banana, Oxidative Stress, Wound Healing.
Food characterization of the spotted pimelodid fish Pimelodus maculatus from a polluted urban river in Argentina David Kuczynski; Juan Carlos Musa; Carla Lorraine Mejías; Maria Florencia D'Alessandro
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.5.2.%x

Abstract

 On the present study, the nourishment and the digestive system of a population of the spotted pimelodid Pimelodus maculatus Lacepède 1803 from a polluted urban river in Argentina were analyzed (Reconquista River, Buenos Aires province). The specimens are characterized by having benthic habits and an omnivore diet. In their intestinal content, a diversity of components was identified. Such components were grouped into ten alimentary items, with dietary variations according to body size while the longitude of the intestine remained proportional to standard length. Microbiological analysis in the gills, stomach and intestine showed the occurrence of Staphylococcus aureus, Escherichia coli and Salmonella sp. Fungi and yeast were also detected. Bacterial accounts were very high both in the analyzed organs as well in water samples. The ecological and sanitary significance of the identified bacteria is discussed.
Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L. Nina Artanti; Muhammad Hanafi; Rina Andriyani; Vienna Saraswaty; Linar Zalinar Udin; Puspa D Lotulung; Ken Ichi Fujita; Yoshinosuke Usuki
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.06

Abstract

Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.
Numerical Study of Predator-Prey Model with Beddington-DeAngelis Functional Response and Prey Harvesting Fira Fitriah; Agus Suryanto; Noor Hidayat
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.09

Abstract

A modified Leslie-Gower predator-prey model with Beddington-DeAngelis functional response and Michaelis-Menten type prey harvesting is studied. The equilibrium points of the system are investigated. To see the stability of each equilibrium point, we perform some numerical simulations. Our numerical simulations show that the extinction of prey or survival of both prey and predator are conditionally stable.
Gametophyte Morphology and Development of Six Species of Pteris (Pteridaceae) from Java Island Indonesia Dwi Sunarti Puspitasari; Tatik Chikmawati; Titien Ngatinem Praptosuwiryo
Journal of Tropical Life Science Vol. 5 No. 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.08

Abstract

The morphology of sporophyte, the type of reproduction, and cytology of Pteris had been reported, while the gametophyte morphology of Pteris in Java island has not been studied yet. The objective of this study was to describe the gametophyte morphology and development of P. biaurita, P. ensiformis,  P.exelsa, P.longipinnula, P. tripartita, and   P. vittata in Java island. Spores were obtained from fertile leaves of Pteris plants originated from several locations in Java island. The number of spores per sporangium were counted from fresh fertile leaves with mature sporangia. As much as 0.002 g spores was sown in transparent box with sterile medium contain of vermiculite, spaghnum moss, and perlite with ratio 2:2:1. The gametophyte development of each species was observed under a microscope every 7 days. The spores of P. ensiformis were germinated faster, 10 days after sowing, while the spores of P.longipinnula were germinated slower, 18 days after sowing.  The pattern of spore germination is Vittaria-type. The development of gametophyte is Ceratopteris-type in common, but in a few cases is the Adiantum-type. The gametophyte development of observed Pteris spesies are varied in six characters including the number of filament cell, germinated time, formation time of notch and gametangia, margin shape, and development type.

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