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Klinikal Sains (Jurnal Analis Kesehatan)
Published by Universitas Abdurrab
ISSN : 23384921     EISSN : 26141515     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology
Jurnal klinikal sains program studi analis kesehatan adalah jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik. Jurnal klinikal sains akan terbit setiap enam bulan, dalam satu periode setiap tahun ajaran akan terbit dua kali yakni pada bulan Juni dan Desember.
Arjuna Subject : -
Articles 119 Documents
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PENGARUH PENGGUNAAN MEDIA ALTERNATIF BEKATUL BERAS MERAH (ORYZA NIVARA) DAN BERAS PUTIH (ORYZA SATIVA L) TERHADAP JUMLAH KOLONI JAMUR CANDIDA ALBICANS DENGAN KONTROL MEDIA SABORAUD DEXTROSE AGAR Bastian Bastian; Gina Septia Rizki; Aristoteles Aristoteles
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3673

Abstract

Jamur Candida albicans membutuhkan sumber nutrisi terutama karbohidrat untuk tumbuh dan berksembang-biak. Identifikasi, isolasi dan konfirmasi hasil pemeriksaan secara mikroskopis memerlukan media kultur. Bekatul beras merah (Oryza nivara) dan bekatul beras putih (Oryza sativa L), bekatul mempunyai karbohidrat sebanyak 84,36%, vitamin, dan nutrisi lainnya. Tujuan penelitian ini untuk mengetahui perbedaan jumlah koloni jamur Candida albicans pada media SDA (Sabouraud Dextrosa Agar), media alternatif bekatul beras merah (Oryza nivara) dan media alternatif bekatul beras putih (Oryza sativa L). Metode penelitian yang digunakan adalah eksperimen murni (true experiment). Penelitian ini dilakukan di laboratorium mikrobiologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang pada tanggal 13 desember 2021 - 17 desember 2021 dengan jumlah sampel sebanyak 30 sampel. Hasil penelitian dengan nilai rata-rata dari hasil pemeriksaan perbedaan jumlah koloni jamur Candida albicans pada media SDA (Sabouraud Dextrosa Agar) 111 CFU/mL, media alternatif bekatul beras merah (Oryza nivara) 146 CFU/mL dan media alternatif bekatul beras putih (Oryza sativa L). Uji ANOVA didapatkan nilai p = < 0,001. Kesimpulan hasil penelitian bahwa Bekatul beras putih dapat digunakan sebagai media alternatif pertumbuhan jamur Candida albicans.
POTENSI FILTRAT UMBI TALAS (Colocasia esculenta (L.) Schott) SEBAGAI ANTIKOAGULAN Maya Herliana Sasmitha; Shabrina Dewi Ramadhani; Muhammad Arsyad; Nafila Nafila; Dian Nurmansyah
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3719

Abstract

A Clinical laboratory is a health laboratory that carries out clinical specimen examination services with the type of blood test material. Blood that is used as an examination material sometimes requires additional substances that can inhibit blood clotting such as anticoagulants. Natural ingredients that are thought to have anticoagulant content are taro tuber filtrate (Colocasia esculenta (L.) Schott). This study aimed to determine the potential of taro tuber filtrate (Colocasia esculenta (L.) Schott) as an alternative anticoagulant. The method used in testing anticoagulant activity is Clotting Time in Vitro. Taro tubers are grated and filtered to obtain filtrate from taro tubers. Taro tuber filtrate test samples are divided into 5 concentrations of 10%, 30%, 50%, 70%, and 90%. Each concentration was carried out 4 times with each treatment given a blood sample of 1 mL. The results of the phytochemical screening test of taro tuber filtrate contain secondary metabolite compounds of flavonoids, alkaloids, and saponins that have anticoagulant activity. Based on the results of clotting time testing that 1 mL of blood mixed with 10% taro tuber filtrate clotted after the 15th hour, while concentrations of 30%, 50%, 70%, and 90% did not occur blood clots after observation for 6 days. Based on the results of research that has been done, it can be concluded that taro tuber filtrate (Colocasia esculenta (L.) Schott) has the potential as an alternative anticoagulant
PROFIL KADAR C-Reactive Protein (CRP), Rheumatoid Factor (RF) DAN LIMFOSIT SEBAGAI INDIKATOR RESPON IMUNITAS PADA LANSIA Anintya Dwi Marrena; Yusuf Eko Nugroho; Meka Faisal Farabi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3759

Abstract

C-Reactive Protein (CRP) is an acute phase protein that is present in normal serum in very small amounts. There are many factors that can affect CRP levels including age, gender, and smoking status. Increasing age allows the emergence of several groups of rheumatic diseases. One of the rheumatism groups that often accompanies the elderly is Rheumatoid Arthritis (RA). Elderly is an age that has a greater chance of experiencing autoimmunity. RA sufferers in the elderly around the world have reached 355 million people, meaning that 1 in 6 elderly people in the world suffer from rheumatism. The resulting human immune response depends on lymphocytes, where systemic inflammation significantly suppresses cellular immunity. Based on the Central Java Health Profile (2019) it shows that the number of elderly people in Cilacap Regency is 174,143 people. This study aims to analyze the number of lymphocytes as an indicator of the immune response in the elderly against levels of CRP, RF. The method used is descriptive qualitative method. From the results of a study of 30 samples in residents of RW 03, Donan Cilacap Village, the results of qualitative examination of CRP and RF levels showed normal results (100%), and the lymphocyte count results obtained for a number of 12 samples showed low results.
DENTIFIKASI CEMARAN BAKTERI Escherichia coli PADA AIR MINUM DI KELURAHAN TLOGOSARI WETAN KOTA SEMARANG Roni Afriansya; Sulistia Istiqomah; Iin Desmiany Duri
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3774

Abstract

Pada tahun 2021 telah dilakukan pemeriksaan bakteri Escherichia coli pada air sumur gali di Kelurahan Tlogosari Wetan hasil menunjukkan bahwa air sumur gali positif mengandung bakteri Escherichia coli. Dengan keberadaan bakteri Escherichia coli pada air sumur gali yang digunakan sebagai sumber air minum, memungkinkan tercemarnya air minum tersebut dengan bakteri Escherichia coli sehingga tidak sesuai dengan Peraturan Menteri Kesehatan Nomor 492/MENKES/Per/IV/2010 yang menyatakan bahwa air minum tidak boleh mengandung bakteri Escherichia coli per 100 ml sampel. Tujuan dari penelitian ini yaitu untuk mengetahui cemaran bakteri Escherichia coli pada air minum di Kelurahan Tlogosari Wetan Kota Semarang. Penelitian ini merupakan penelitian observasional dengan kriteria deskriptif melalui pendekatan cross sectional menggunakan metode membran filter. Berdasarkan pemeriksaan bakteri Escherichia coli metode membran filter yang dilakukan pada 10 sampel air minum diperoleh 3 sampel positif mengandung bakteri Escherichia coli, sedangkan 7 sampel lainnya negatif. Kesimpulan dari penelitian ini yaitu Dari 10 sampel air minum yang diperiksa, ditemukan 3 sampel terkontaminasi bakteri Escherichia coli dan 7 sampel lainnya tidak mengandung bakteri Escherichia coli.
PENGARUH VARIASI WAKTU PENYIMPANAN TERHADAP STABILITAS REAGEN KERJA PADA HASIL PEMERIKSAAN KADAR KREATININ Silvi Marshela; Suryanata Kesuma; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3809

Abstract

Kidney is one of the organs of the body whose role is very important to maintain the stability of volume, osmolarity of extracellular fluid, and electrolyte composition. One of the parameters to determine kidney function that is usually done is creatinine examination. Preparation of working reagents is very important for accurate results in an examination. The purpose of this study was to determine the effect of storage on the stability of working reagents on creatinine examination. This type of research is a pseudo-experiment, to determine the difference between working reagents that are directly used with working reagents that are stored for several days. The research variables are the length of storage of work reagents, creatinine levels and storage temperature. The sample used in this study was normal creatinine control. The results were analyzed using Microsoft Excel and using the Kruskal Wallis Non Parametric test. The results of the study showed that the average reagent immediately and 5 days was 0.8 mg/dl and at 8 days 1.2 mg/dl, the accuracy obtained was -3.2% and 0.4%, the precision value obtained was 35.1% and 31.0%, in statistical tests significant results <0.05, meaning that there are differences in the results of examinations using working reagents that are used immediately and those stored for 5 and 8 days.
ANALISIS FAKTOR PENULARAN VIRUS HEPATITIS B KONTAK SERUMAH DI DI DESA BONE KABUPATEN KUPANG Agustina Welhelmina Djuma; Diana Ayu Eiga Julianty; Wilhelmus Olin; Agustina Setia
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3818

Abstract

Virus Hepatitis B (VHB) dari famili Hepadnaviridae merupakan penyebab terjadinya penyakit Hepatitis B. Pajanan VHB menyebabkan keluaran klinis yaitu hepatitis akut dan Hepatitis kronik. Hepatitis akut akan sembuh dan membentuk kekebalan terhadap penyakit ini, sedangkan hepatitis yang berkembang menjadi kronik akan berakibat pada peradangan hati akut atau menahun. Pada beberapa kasus perkembangan peradangan hati akut ataupun menahun dapat menjadi sirosis hati. Virus Hepatitis B dtransmisikan secara vertikal dan horizontal. Penularan dari ibu kepada janinya melalui plasenta pada masa perinatal merupakan penularan vertikal, sedangkan penularan melalui cairan tubuh penderita Hepatitis B antara lain air liur, darah, atau cairan tubuh dan kontak seksual merupakan jenis penularan secara horizontal. Penelitian ini bertujuan menganalisis karakteristik, perilaku berisiko dan tingkat pengetahuan dari anggota keluarga yang kontak serumah dengan penderita Hepatitis B di Desa Bone. Jenis penelitian ini observasional analitik menggunakan rancangan cross sectional. Perilaku berisiko dan tingkat pengetahuan diukur menggunakan kuisioner. Analisis Chi-Square untuk mengkaji faktor yang berhubungan dengan penularan Hepatitis B. Responden pada penelitian ini berjumlah 40 orang. Hasil penelitian menunjukkan 37,5% responden dengan HBsAg positif terdapat pada laki-laki 20% dan 17,5% pada perempuan. Responden dengan HBsAg positif terbanyak pada kelompok usia 26-45 tahun sebesar 27,5%. Usia merupakan faktor yang berhubungan dengan Hepatitis B (p<0,05). Tidak ditemukan adanya hubungan antara beberapa kebiasaan yang dapat berisiko menularkan VHB, yaitu penggunaan alat pribadi bersama (p>0,05) dengan kejadian Hepatitis B. Meskipun tingkat pengetahuan masyarakat tentang Hepatitis B tergolong rendah (69,4%), namun tingkat pengetahuan responden di Desa Bone tidak merupakan faktor yang ada hubungannya dengan kejadian Hepatitis B.
IDENTIFIKASI BAKTERI Escherichia coli, Shigella sp. DAN Staphylococcus aureus SEBAGAI BIOMARKER CEMARAN PADA SUMBER AIR DAERAH STUNTING DI KELURAHAN BELAWAN SICANANG, KECAMATAN MEDAN BELAWAN, MEDAN Tania Regita Sari; Dinda Aulia Habib; Febri Sembiring; Gabriella Septiani Nasution; Lestari Rahmah
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3892

Abstract

Stunting is a disorder of growth and development of children due to chronic malnutrition and repeated infections, which are characterized by below standard height. Water is a kind of resource which required for community household activities,. Therefore, water, sanitation and hygiene become particular concern. Contamination by Enterotoxigenic Escherichia coli that transmitted through water can increase the risk of stunting in children with continuous diarrhea. This research was conducted to identify biomarkers in water sources for local residents with stunting in Belawan Sicanang Village, Medan. The biomarkers used were E. coli, Shigella sp., and Staphylococcus aureus. The method used in this study is the Most Probable Number (MPN) and Total Plate Count (TPC). The results of this study indicated that 2 out of 3 drinking water samples were contaminated with E. coli., and 3 out of 3 clean water samples were contaminated with E. coli. Meanwhile, the other two biomarkers were not detected in all types of samples.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli, Salmonella thypi, Staphylococcus aureus dan Staphylococcus epidermidis yuli yantika syahputri; Febri Sembiring; Gabriella Septiani Nasution; Nita Andriani Lubis
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3894

Abstract

Noni fruit (Morinda citrifolia L.) is one part of the Noni plant that contains metabolite compounds that are antibacterial. The antibacterial compounds produced are known to inhibit the growth of disease-causing bacteria. The purpose of this study was to measure the antibacterial activity of various concentrations of noni fruit extract against the growth of Escherichia coli, Salmonella thypi, Staphylococcus aureus and Staphylococcus epidermidis in vitro using the disc diffusion method. The results of this study indicate that the lowest concentration of Noni Fruit extract (60% v/v) can inhibit the growth of all types of bacteria tested. From the four test bacteria, the largest inhibition zone was found on Escherichia coli with a size of 23.05 mm at a concentration of 80% v/v Noni Fruit extract.
PENGARUH VARIASI WAKTU PENDINGINAN CARBOL FUCHSIN TERHADAP HASIL PEMERIKSAAN BASIL TAHAN ASAM Amelia Maulida; Tiara Dini Harlita; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3906

Abstract

Microscopic examination with Ziehl Neelsen staining is one way to confirm the diagnosis of tuberculosis. Ziehl Neelsen staining has 3 reagents, namely carbol fuchsin 1%, alcohol acid 3%, and methylene blue 0.1%. Based on Standard Operating Procedures, the carbol fuchsin cooling process is carried out for 5-7 minutes. The aim of this research was to determine the effect of varying carbol fuchsin cooling times of 6 minutes, 8 minutes, 10 minutes and 12 minutes on the results of BTA microscopic examination. This type of research is an experiment with a completely randomized design (CRD). The samples used were BTA (+3) sputum specimens from RSUD. Abdoel Wahab Sjahranie 3 pots. The quality assessment of BTA preparations was carried out by the East Kalimantan Provincial Health Laboratory. BTA preparations were given 4 treatments varying in carbol fuchsin cooling time, namely 6 minutes, 8 minutes, 10 minutes and 12 minutes, then repeated 6 times and analyzed using the Chi-Squared test. The results of this study were that all BTA preparations that were treated with variations in carbol fuchsin cooling time were of good quality based on 6 criteria for good preparations with an average scoring of 100% in the 6 minute treatment, 98.3% in the 8 minute treatment, 96.6% in the 8 minute treatment. 10 minutes and 95% in the 12 minute treatment. Based on the results of the Chi-Squared test analysis of cleanliness criteria, a p value of 0.000 was obtained, indicating that there was no significant influence between variations in carbol fuchsin cooling time on the results of BTA microscopic examination. Thus, during the cooling stage, carbol fuchsin can be used for a duration of 5-7 minutes to produce a good quality preparation.

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