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All Journal Pastura; Journal Of Tropical Forage Science Animal Production : Indonesian Journal of Animal Production Jurnal Entomologi Indonesia
Toar, Wisje Lusia
Fakultas Peternakan Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary Other

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Insect Crude Thoraxial Antigen-G Extracted from Apis mellifera to Enhance Serum Immunoglobulin of Goats: An Entomology Contribution in Animal Science Toar, Wisje Lusia; Rumokoy, Laurentius; Untu, Ivonne Maria; Assa, Geertruida
ANIMAL PRODUCTION Vol 20, No 2 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.925 KB) | DOI: 10.20884/1.jap.2018.20.2.608

Abstract

This research was conducted to evaluate the influence of insect crude thoraxial antigen-G (CTA) extracted from Apis mellifera L. (Hymenoptera: Apidae) to enhance goat’s serum immunoglobulin level. The first part of this study was the determination of insect CTA proportion level. The insects were collected from four different places: Tomohon, Minahasa, North-Minahasa and Manado areas. The second part of the study was the application of A. mellifera CTA substance on serum immunoglobulin level classification. In this part, twelve young goats handled with traditional maintenance.  The animals experiment were divided in two groups: control group and the other treated with 100 µg CTA extract. The proportion of serum immunoglobulins level of goats was detected at 14th days after immunization with insects CTA extract, and compared with the animals immunoglobulin levels at the starting day of treatment.  The data of CTA extract proportion level of the insects collected were subjected to statistically analysis using the general linear model (GLM) procedure of SPSS 22. Concerning the classification level of the animal treated with CTA was statistically analyzed according to Mann-Whitney test. The results showed that the proportion level of thoraxial antigens-G of A. mellifera from all areas observed were not significant different (P>0.05). This crude thoraxial antigens-G of this insect were able to increase serum antibody level of the experiment animal after 14 days of immunization. The immunoglobulin level qualification of animals in treated group were significant higher (P<0.05) than in control group. We concluded that the CTA extract of the Apis mellifera could be empowered to improve the young goat immunity against the pathogenic microbes in their environment. 
THE EFFECTS OF PAPAIN CRUDE EXTRACT ADDITION IN DIETS ON BROILERS PRODUCTION PERFORMANCES Rumokoy, Laurentius; Pudjihastuti, Endang; Untu, Ivonne Maria; Toar, Wisje Lusia
ANIMAL PRODUCTION Vol 18, No 1 (2016): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.339 KB) | DOI: 10.20884/1.anprod.2016.18.1.540

Abstract

This study was conducted to evaluate the effects of papain crude extract addition in mash and pellet feed forms on production performance of broiler chickens in order to obtain the best level of extract papain in mash or pellet form. This natural protease enzyme was extracted from unripe papaya. A complete random design was applied in this study and it was arranged with factorial 4 * 2 and three replications. The treatments were 4 levels of papain (0, 0.03, 0.05, and 0.07 %) and two physical forms of feed (mash and pellet). Broilers production parameters measured were: feed intake, body weight, feed conversion ratio (FCR) and carcass percentage. The results of analysis of variance showed that the interaction was highly significant (P <0.01) for feed intake, body weight, carcass percentage respectively while feed conversion showed significant interaction (P <0.05). The significant differences in the feed consumption described the role of papain enzyme through treatment of CEP and the physical form of feed. The results indicate that the all treatment of papain crude extract level  both in mash and pellet feed form were able to improve feed intake, body weight, FCR and carcass percentage of broiler chickens, whereas the best performance was obtained in the treatment of 0.05% papain crude extract in mash form of diets.
Antigen-G of Larva Serum of Musca domestica as an Immunoglobulin Production Promoter in Goats under an Organic Maintenance Rumokoy, Laurentius; Kiroh, Hengky; Untu, Ivonne Maria; Assa, Geertruida; Turangan, Santie; Toar, Wisje Lusia
ANIMAL PRODUCTION Vol. 22 No. 1 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.576 KB) | DOI: 10.20884/1.jap.2020.22.1.36

Abstract

This experiment aimed to reveal the effect of serum G-antigen of M. domesticainsect larvae (LAS) as promoter antigen on serum immunoglobulin production in organically managed goat livestock. This study used 12 local goats which were divided into two groups: as a control group and treated group receiving treatment. Insect rearing was conducted to obtain the larvae, the antigen-G was then extracted from the larvae to be used as promoter antigen to enhance the serum antibody production which was subcutaneously immunized in experimental goats and incubated for a period of 14 days. Blood collection of 2.5 ml was taken through the jugular vein and then carried out a quantification of serum protein level indicating the antibody proportion of goats. The data of the LSA extract proportion level were statistically analyzed with t-test, and the quality classification level of serum immunoglobulin of animal groups were statistically analysed. The results showed that the serum of animal treated with LSA of M. domesticaresulted in a higher level of immunoglobulin (P <0.01) compared to the control. We conclude that the antigen-g substance (LSA) could support the efforts to improve the production of organic goat livestock by increasing the total level of antibodies circulating in the blood.
KERAGAMAN SERANGGA PADA BUDIDAYA Brachiaria mutica DAN POTENSI REVITALISASI LAHAN DI BAWAH KANOPI KELAPA Laurentius J. M. Rumokoy; Constatyn Sumolang; Ivonne Maria Untu; Wisje Lusia Toar
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 10 No 2 (2021): Pastura Vol. 10 No. 2 Tahun 2021
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/Pastura.2021.v10.i02.p03

Abstract

Penelitian observasi ini bertujuan untuk mencari tahu kondisi lahan kelapa yang berpotensi direvitalisasi.Secara simultan penelitian ini memiliki tujuan lain untuk mencari tahu keragaman serangga di lokasi budidayaBrachiaria mutica di bawah kanopi pohon kelapa. Metode yang digunakan dalam studi ini terbagi dalam duatahap, pertama observasi keragaman serangga dilakukan pada lokasi budidaya B. mutica di bawah kanopikelapa dilakukan di Sentrum Agraris Lotta (SAL), tahap kedua adalah survei lapangan menyangkut levelkualitas lahan di bawah areal tanaman kelapa di daerah perbatasan Manado dan Minahasa Utara. Hasilpenelitian menunjukkan bahwa ada enam ordo dan 10 famili serangga dengan indeks keragaman sebesar0,323 SWI dengan pola interaksi positif antara serangga-serangga dengan B. mutica yang ditanam di bawah tanaman kelapa. Tedapat 37,5% lahan kelapa yang tergolong Q3. Kondisi kualitas lahan terbanyak pada level Q3 dan Q2 menunjukkan bahwa upaya revitalisasi dibutuhkan untuk dapat memaksimumkan lahandi bawah kanopi kelapa. Dengan demikian dapat disimpulkan bahwa upaya revitalisasi pemanfaatan lahandi bawah kanopi tanaman kelapa perlu dilakukan dengan mempertimbangkan berbagai kondisi lahan danperanan serangga yang berinteraksi dengan tanaman budidaya tersebut.Kata kunci interaksi serangga, Brachiaria mutica, revitalisasi, lahan kelapa
Efek ekstrak minyak citronela Cymbopogon nardus L. terhadap proporsi frekuensi kontak fisik Musca domestica L. (Diptera: Muscidae) pada ransum dan performa broiler Laurentius Rumokoy; Charles Kaunang; Wisje Toar
Jurnal Entomologi Indonesia Vol 14 No 2 (2017): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.566 KB) | DOI: 10.5994/jei.14.2.89

Abstract

Flies are well known as vector that has an important role in transferring pathogen microbes to broilers. Various tropical plants, as well as Cymbopogon spp. possess a large potential in controlling the activities of this flies. This research was aimed to determine the effect of citronella oils extracted from Cymbopogon nardus L on  Musca domestica L. (Diptera: Muscidae) activities in ration surface and also to study the effect of COE in ration on broiler performances. Three treatment levels of EMC were 0%, 0.25%, and 0.5% and added in broilers ration experimental. The research was designed by using randomized experimental design. The parameters measured were: proportion of physical contact frequency (PCF), feed consumption, and body weight gain. The results showed that the treatment levels of COE of C. nardus significantly influenced (F2,48 = 38,456, P=0,000) PCF of M. domestica, while the levels of COE were significantly influencing (F2,36 = 51,178, P = 0,000) the feed consumption  and  body weight gain of broilers (F2,36 = 81,969, P=0,000). This research revealed that the levels of COE of C. nardus were able to decrease PCF value of M. domestica in ration while the treatments increased feed consumption and body gain values of broilers. We concluded that EMC of C. nardus L up to 0.5% can function as bio-repellent to the M. domestica activities on broiler ration and cause a positive effect to the consumption and to the body gains of chickens broilers.
Application of Thoracic Immunogen of Musca domestica on Immunoglobulin-G Level of Goats Detected Through a Single Radial Immuno-Diffusion Test Laurentius Rumokoy; Wisje Lusia Toar
ANIMAL PRODUCTION Vol. 24 No. 1 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.1.76

Abstract

The purpose of this study was to detect the IgG level of goats treated with immunogen thoracic of Musca domestica (ITMd). We reared twelve young goats aged two months old under extensive treatments, divided into two equal groups for a comparison experimental design. The animals in the first group (R0) served as the control without immunogen injection, and the second group (R1) was the treatment group receiving a subcutaneous injection of 10 mL of thoracic antigens per head. The parameter observed was IgG (immunoglobulin-G) antibody serum level. The quantification of goat immunoglobulins was carried out using a single radial immuno-diffusion (SRID) technique. The data obtained from the two groups were analyzed with a t-student test. The results showed that the total IgG antibody serum of goats in the treatment group was significantly higher than that of the control group (P< 0.05). This difference may be due to the thoracic M. domestica immunogen response which increased antibody synthesis of experiment goats. We concluded that the treatment of thoracic immunogen of M. domestica at a level of 10 µL injected subcutaneously could significantly increase IgG antibodies in goat blood which were detected using a single radial immuno-diffusion method.
Co-Authors Assa, Geertruida Assa, Geertruida Charles Kaunang Constatyn Sumolang Endang Pudjihastuti, Endang Ivonne Maria Untu Kiroh, Hengky Laurentius J. M. Rumokoy Laurentius Rumokoy Laurentius Rumokoy Laurentius Rumokoy, Laurentius Tiene M. B.Turangan Untu, Ivonne Maria Untu, Ivonne Maria